Restriction map for
pFN21ASDA0352
(Linear) (Six-base) MAPSORT of: KIAA0352.seq Check: 3226 from: 1 to: 2136
KIAA0352.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AatII G_ACGT'C
Cuts at: 0 95 2136
Size: 95 2041
AccI GT'mk_AC
Cuts at: 0 260 800 2136
Size: 260 540 1336
Fragments arranged by size:
1336 540 260
Acc65I G'GTAC_C
Cuts at: 0 427 2136
Size: 427 1709
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 251 747 2136
Size: 70 181 496 1389
Fragments arranged by size:
1389 496 181 70
AflIII A'CryG_T
Cuts at: 0 1208 2034 2136
Size: 1208 826 102
Fragments arranged by size:
1208 826 102
AleI CACnn'nnGTG
Cuts at: 0 100 2136
Size: 100 2036
AlwNI CAG_nnn'CTG
Cuts at: 0 454 1451 1651 2136
Size: 454 997 200 485
Fragments arranged by size:
997 485 454 200
ApaI G_GGCC'C
Cuts at: 0 1974 2136
Size: 1974 162
ApaLI G'TGCA_C
Cuts at: 0 1585 2044 2136
Size: 1585 459 92
Fragments arranged by size:
1585 459 92
ApoI r'AATT_y
Cuts at: 0 1855 2136
Size: 1855 281
AvaI C'yCGr_G
Cuts at: 0 886 1088 1157 1965 2136
Size: 886 202 69 808 171
Fragments arranged by size:
886 808 202 171 69
AvrII C'CTAG_G
Cuts at: 0 687 2136
Size: 687 1449
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 411 444 2136
Size: 411 33 1692
Fragments arranged by size:
1692 411 33
BamHI G'GATC_C
Cuts at: 0 1874 2136
Size: 1874 262
BanI G'GyrC_C
Cuts at: 0 427 2136
Size: 427 1709
BanII G_rGCy'C
Cuts at: 0 727 881 1061 1974 2136
Size: 727 154 180 913 162
Fragments arranged by size:
913 727 180 162 154
BbsI GAAGACnn'nnnn_
Cuts at: 0 635 926 1512 1607 2136
Size: 635 291 586 95 529
Fragments arranged by size:
635 586 529 291 95
BbvCI CC'TCA_GC
Cuts at: 0 929 2136
Size: 929 1207
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1069 1103 2136
Size: 1069 34 1033
Fragments arranged by size:
1069 1033 34
BclI T'GATC_A
Cuts at: 0 287 2136
Size: 287 1849
BglI GCCn_nnn'nGGC
Cuts at: 0 133 2136
Size: 133 2003
BlpI GC'TnA_GC
Cuts at: 0 317 2136
Size: 317 1819
Bme1580I G_kGCm'C
Cuts at: 0 1589 1974 2003 2048 2136
Size: 1589 385 29 45 88
Fragments arranged by size:
1589 385 88 45 29
BmgBI CAC'GTC
Cuts at: 0 656 1668 2136
Size: 656 1012 468
Fragments arranged by size:
1012 656 468
BmrI ACTGGGnnnn_n'
Cuts at: 0 194 518 821 1227 2136
Size: 194 324 303 406 909
Fragments arranged by size:
909 406 324 303 194
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 438 2136
Size: 438 1698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 394 554 865 1720 2136
Size: 328 66 160 311 855 416
Fragments arranged by size:
855 416 328 311 160 66
Bpu10I CC'TnA_GC
Cuts at: 0 929 2136
Size: 929 1207
BsaI GGTCTCn'nnnn_
Cuts at: 0 73 1126 1327 2136
Size: 73 1053 201 809
Fragments arranged by size:
1053 809 201 73
BsaHI Gr'CG_yC
Cuts at: 0 92 1604 2136
Size: 92 1512 532
Fragments arranged by size:
1512 532 92
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 630 660 1138 1168 2136
Size: 630 30 478 30 968
Fragments arranged by size:
968 630 478 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 331 964 1774 2136
Size: 331 633 810 362
Fragments arranged by size:
810 633 362 331
BseYI C'CCAG_C
Cuts at: 0 677 1317 1728 2136
Size: 677 640 411 408
Fragments arranged by size:
677 640 411 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 1647 1971 2136
Size: 170 1477 324 165
Fragments arranged by size:
1477 324 170 165
BsiHKAI G_wGCw'C
Cuts at: 0 382 727 845 881 1589 2048 2136
Size: 382 345 118 36 708 459 88
Fragments arranged by size:
708 459 382 345 118 88 36
Bsp1286I G_dGCh'C
Cuts at: 0 382 727 845 881 1061 1589 1974 2003
Size: 382 345 118 36 180 528 385 29
Cuts at: 2003 2048 2136
Size: 45 88
Fragments arranged by size:
528 385 382 345 180 118 88 45
36 29
BspHI T'CATG_A
Cuts at: 0 2024 2136
Size: 2024 112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 53 462 1112 2136
Size: 53 409 650 1024
Fragments arranged by size:
1024 650 409 53
BsrBI CCG'CTC
Cuts at: 0 116 1934 2136
Size: 116 1818 202
Fragments arranged by size:
1818 202 116
BsrDI GCAATG_nn'
Cuts at: 0 759 2136
Size: 759 1377
BsrFI r'CCGG_y
Cuts at: 0 68 1868 2136
Size: 68 1800 268
Fragments arranged by size:
1800 268 68
BsrGI T'GTAC_A
Cuts at: 0 2093 2136
Size: 2093 43
BssSI C'ACGA_G
Cuts at: 0 720 2136
Size: 720 1416
BstAPI GCAn_nnn'nTGC
Cuts at: 0 454 1127 1451 2136
Size: 454 673 324 685
Fragments arranged by size:
685 673 454 324
BstXI CCAn_nnnn'nTGG
Cuts at: 0 105 466 2136
Size: 105 361 1670
Fragments arranged by size:
1670 361 105
BstYI r'GATC_y
Cuts at: 0 852 1874 2136
Size: 852 1022 262
Fragments arranged by size:
1022 852 262
BtsI GCAGTG_nn'
Cuts at: 0 380 1452 1614 2136
Size: 380 1072 162 522
Fragments arranged by size:
1072 522 380 162
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1809 1844 2136
Size: 1809 35 292
Fragments arranged by size:
1809 292 35
EaeI y'GGCC_r
Cuts at: 0 1379 2136
Size: 1379 757
EarI CTCTTCn'nnn_
Cuts at: 0 178 280 548 1615 1769 2136
Size: 178 102 268 1067 154 367
Fragments arranged by size:
1067 367 268 178 154 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1887 2131 2136
Size: 1887 244 5
Fragments arranged by size:
1887 244 5
EcoICRI GAG'CTC
Cuts at: 0 725 879 2136
Size: 725 154 1257
Fragments arranged by size:
1257 725 154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 251 328 394 554 747 865 1720
Size: 70 181 77 66 160 193 118 855
Cuts at: 1720 2136
Size: 416
Fragments arranged by size:
855 416 193 181 160 118 77 70
66
EcoNI CCTnn'n_nnAGG
Cuts at: 0 49 343 2136
Size: 49 294 1793
Fragments arranged by size:
1793 294 49
EcoO109I rG'GnC_Cy
Cuts at: 0 203 336 891 1970 1971 2136
Size: 203 133 555 1079 1 165
Fragments arranged by size:
1079 555 203 165 133 1
EcoRI G'AATT_C
Cuts at: 0 1855 2136
Size: 1855 281
HaeII r_GCGC'y
Cuts at: 0 1786 2136
Size: 1786 350
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1138 1170 2136
Size: 1138 32 966
Fragments arranged by size:
1138 966 32
KpnI G_GTAC'C
Cuts at: 0 431 2136
Size: 431 1705
MscI TGG'CCA
Cuts at: 0 1381 2136
Size: 1381 755
MslI CAynn'nnrTG
Cuts at: 0 100 103 1582 1899 2039 2136
Size: 100 3 1479 317 140 97
Fragments arranged by size:
1479 317 140 100 97 3
MspA1I CmG'CkG
Cuts at: 0 154 1242 1985 2136
Size: 154 1088 743 151
Fragments arranged by size:
1088 743 154 151
NaeI GCC'GGC
Cuts at: 0 70 2136
Size: 70 2066
NgoMIV G'CCGG_C
Cuts at: 0 68 2136
Size: 68 2068
NspI r_CATG'y
Cuts at: 0 1087 1212 2038 2136
Size: 1087 125 826 98
Fragments arranged by size:
1087 826 125 98
PciI A'CATG_T
Cuts at: 0 1208 2034 2136
Size: 1208 826 102
Fragments arranged by size:
1208 826 102
PflMI CCAn_nnn'nTGG
Cuts at: 0 1596 2136
Size: 1596 540
PfoI T'CCnGG_A
Cuts at: 0 1142 2136
Size: 1142 994
PpuMI rG'GwC_Cy
Cuts at: 0 203 336 891 2136
Size: 203 133 555 1245
Fragments arranged by size:
1245 555 203 133
PshAI GACnn'nnGTC
Cuts at: 0 1460 2136
Size: 1460 676
PspOMI G'GGCC_C
Cuts at: 0 1970 2136
Size: 1970 166
PspXI vC'TCGA_Gb
Cuts at: 0 886 2136
Size: 886 1250
PstI C_TGCA'G
Cuts at: 0 1447 1480 1724 1790 2136
Size: 1447 33 244 66 346
Fragments arranged by size:
1447 346 244 66 33
PvuII CAG'CTG
Cuts at: 0 154 1242 2136
Size: 154 1088 894
Fragments arranged by size:
1088 894 154
SacI G_AGCT'C
Cuts at: 0 727 881 2136
Size: 727 154 1255
Fragments arranged by size:
1255 727 154
SapI GCTCTTCn'nnn_
Cuts at: 0 1769 2136
Size: 1769 367
SfcI C'TryA_G
Cuts at: 0 1443 1476 1720 1786 2004 2136
Size: 1443 33 244 66 218 132
Fragments arranged by size:
1443 244 218 132 66 33
SgrAI Cr'CCGG_yG
Cuts at: 0 1868 2136
Size: 1868 268
SmaI CCC'GGG
Cuts at: 0 1159 2136
Size: 1159 977
SmlI C'TyrA_G
Cuts at: 0 886 2136
Size: 886 1250
SphI G_CATG'C
Cuts at: 0 1087 2136
Size: 1087 1049
SspI AAT'ATT
Cuts at: 0 1268 2136
Size: 1268 868
StuI AGG'CCT
Cuts at: 0 349 1441 2136
Size: 349 1092 695
Fragments arranged by size:
1092 695 349
StyI C'CwwG_G
Cuts at: 0 465 495 687 2136
Size: 465 30 192 1449
Fragments arranged by size:
1449 465 192 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1163 1742 1759 2136
Size: 1163 579 17 377
Fragments arranged by size:
1163 579 377 17
TatI w'GTAC_w
Cuts at: 0 2093 2136
Size: 2093 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 630 645 662 677 1483 1515 1993 2025
Size: 630 15 17 15 806 32 478 32
Cuts at: 2025 2136
Size: 111
Fragments arranged by size:
806 630 478 111 32 32 17 15
15
XbaI T'CTAG_A
Cuts at: 0 1053 1566 2136
Size: 1053 513 570
Fragments arranged by size:
1053 570 513
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 207 2136
Size: 207 1929
XhoI C'TCGA_G
Cuts at: 0 886 2136
Size: 886 1250
XmaI C'CCGG_G
Cuts at: 0 1157 2136
Size: 1157 979
XmnI GAAnn'nnTTC
Cuts at: 0 172 2136
Size: 172 1964
ZraI GAC'GTC
Cuts at: 0 93 2136
Size: 93 2043
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII AleI AlwNI ApaI
ApaLI ApoI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BclI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstXI BstYI BtsI CspCI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
HaeII Hin4I KpnI MscI MslI MspA1I NaeI NgoMIV
NspI PciI PflMI PfoI PpuMI PshAI PspOMI PspXI
PstI PvuII SacI SapI SfcI SgrAI SmaI SmlI
SphI SspI StuI StyI TaqII TatI TstI XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AgeI AhdI AlfI AloI
AscI AseI AsiSI BbeI BciVI BfrBI BglII BmtI
BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BssHII BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
ClaI DraI DraIII DrdI EagI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI MfeI MluI
MmeI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PmeI PmlI PpiI PsiI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SnaBI SpeI SrfI SwaI Tth111I