(Circular) (Six-base) MAPSORT of: pFN21AE3703.seq Check: 225 from: 1 to: 9742 pFN21AE3703 9742 bp With 182 enzymes: SgfI * March 28, 2012 14:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5421 7552 278 Size: 53 83 186 936 3885 2131 2468 Fragments arranged by size: 3885 2468 2131 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 7085 1937 Size: 5148 4594 Acc65I G'GTAC_C Cuts at: 7063 7063 Size: 9742 AclI AA'CG_TT Cuts at: 1563 7869 8242 1563 Size: 6306 373 3063 Fragments arranged by size: 6306 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3844 4190 5285 5324 7805 9189 9457 1632 Size: 2212 346 1095 39 2481 1384 268 1917 Fragments arranged by size: 2481 2212 1917 1384 1095 346 268 39 AfeI AGC'GCT Cuts at: 6262 6262 Size: 9742 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9545 AflIII A'CryG_T Cuts at: 1203 6543 6783 8641 9618 1203 Size: 5340 240 1858 977 1327 Fragments arranged by size: 5340 1858 1327 977 240 AgeI A'CCGG_T Cuts at: 8573 8573 Size: 9742 AhdI GACnn_n'nnGTC Cuts at: 8471 8471 Size: 9742 AleI CACnn'nnGTG Cuts at: 1166 2370 5738 6902 1166 Size: 1204 3368 1164 4006 Fragments arranged by size: 4006 3368 1204 1164 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2874 5756 1184 Size: 1690 2882 5170 Fragments arranged by size: 5170 2882 1690 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6506 6538 6506 Size: 32 9710 AlwNI CAG_nnn'CTG Cuts at: 5162 5198 5406 6034 7485 9057 5162 Size: 36 208 628 1451 1572 5847 Fragments arranged by size: 5847 1572 1451 628 208 36 ApaI G_GGCC'C Cuts at: 2035 2439 2870 3315 4488 4626 4633 4670 5292 Size: 404 431 445 1173 138 7 37 622 Cuts at: 5292 6456 6913 2035 Size: 1164 457 4864 Fragments arranged by size: 4864 1173 1164 622 457 445 431 404 138 37 7 ApaLI G'TGCA_C Cuts at: 6233 6401 7799 8955 6233 Size: 168 1398 1156 7020 Fragments arranged by size: 7020 1398 1156 168 ApoI r'AATT_y Cuts at: 1493 4055 7051 7277 7429 9538 1493 Size: 2562 2996 226 152 2109 1697 Fragments arranged by size: 2996 2562 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6387 8296 160 Size: 872 5355 1909 1606 Fragments arranged by size: 5355 1909 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9742 AvaI C'yCGr_G Cuts at: 1870 1943 3477 3908 3968 3990 4632 4663 5217 Size: 73 1534 431 60 22 642 31 554 Cuts at: 5217 5526 7067 1870 Size: 309 1541 4545 Fragments arranged by size: 4545 1541 1534 642 554 431 309 73 60 31 22 BamHI G'GATC_C Cuts at: 7072 7439 7072 Size: 367 9375 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2211 2242 3179 3864 5981 Size: 325 217 627 424 31 937 685 2117 Cuts at: 5981 6139 6816 7063 8518 618 Size: 158 677 247 1455 1842 Fragments arranged by size: 2117 1842 1455 937 685 677 627 424 325 247 217 158 31 BanII G_rGCy'C Cuts at: 1391 2035 2093 2439 2870 3315 3352 3507 3878 Size: 644 58 346 431 445 37 155 371 Cuts at: 3878 4179 4488 4574 4626 4633 4670 5292 5657 Size: 301 309 86 52 7 37 622 365 Cuts at: 5657 5807 6456 6913 6977 6999 7061 1391 Size: 150 649 457 64 22 62 4072 Fragments arranged by size: 4072 649 644 622 457 445 431 371 365 346 309 301 155 150 86 64 62 58 52 37 37 22 7 BbeI G_GCGC'C Cuts at: 2246 3868 6820 2246 Size: 1622 2952 5168 Fragments arranged by size: 5168 2952 1622 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 8771 BbvCI CC'TCA_GC Cuts at: 2672 4549 2672 Size: 1877 7865 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5971 6005 6791 6825 7933 Size: 34 50 34 3950 34 786 34 1108 Cuts at: 7933 7967 1903 Size: 34 3678 Fragments arranged by size: 3950 3678 1108 786 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7634 8844 7634 Size: 1210 8532 BclI T'GATC_A Cuts at: 1548 6600 1548 Size: 5052 4690 BfrBI ATG'CAT Cuts at: 2008 6332 2008 Size: 4324 5418 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2682 3177 3470 Size: 107 122 71 631 605 1010 495 293 Cuts at: 3470 5123 5652 6093 6837 6917 8353 136 Size: 1653 529 441 744 80 1436 1525 Fragments arranged by size: 1653 1525 1436 1010 744 631 605 529 495 441 293 122 107 80 71 BglII A'GATC_T Cuts at: 3492 5787 9737 3492 Size: 2295 3950 3497 Fragments arranged by size: 3950 3497 2295 BlpI GC'TnA_GC Cuts at: 2146 2427 6006 6083 7159 2146 Size: 281 3579 77 1076 4729 Fragments arranged by size: 4729 3579 1076 281 77 Bme1580I G_kGCm'C Cuts at: 1790 2035 2214 2439 2870 3315 3605 4488 4626 Size: 245 179 225 431 445 290 883 138 Cuts at: 4626 4633 4670 5292 6144 6237 6405 6456 6534 Size: 7 37 622 852 93 168 51 78 Cuts at: 6534 6814 6913 6943 7803 8959 1790 Size: 280 99 30 860 1156 2573 Fragments arranged by size: 2573 1156 883 860 852 622 445 431 290 280 245 225 179 168 138 99 93 78 51 37 30 7 BmgBI CAC'GTC Cuts at: 1318 7035 1318 Size: 5717 4025 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2609 4802 5479 Size: 416 53 83 399 24 1192 2193 677 Cuts at: 5479 5773 6256 6415 6953 7473 8431 442 Size: 294 483 159 538 520 958 1753 Fragments arranged by size: 2193 1753 1192 958 677 538 520 483 416 399 294 159 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9742 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2010 2107 2154 2220 2543 2700 2931 3094 Size: 771 97 47 66 323 157 231 163 Cuts at: 3094 3305 3801 4049 4448 4479 5127 5585 6872 Size: 211 496 248 399 31 648 458 1287 Cuts at: 6872 8402 1239 Size: 1530 2579 Fragments arranged by size: 2579 1530 1287 771 648 496 458 399 323 248 231 211 163 157 97 66 47 31 Bpu10I CC'TnA_GC Cuts at: 2069 2672 4549 6879 6993 2069 Size: 603 1877 2330 114 4818 Fragments arranged by size: 4818 2330 1877 603 114 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2570 7867 8732 9030 2570 Size: 5297 865 298 3282 Fragments arranged by size: 5297 3282 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3227 3295 3895 4754 5336 6245 6366 Size: 617 1728 68 600 859 582 909 121 Cuts at: 6366 8405 882 Size: 2039 2219 Fragments arranged by size: 2219 2039 1728 909 859 617 600 582 121 68 BsaAI yAC'GTr Cuts at: 493 1204 6091 6544 6786 7533 493 Size: 711 4887 453 242 747 2702 Fragments arranged by size: 4887 2702 747 711 453 242 BsaBI GATnn'nnATC Cuts at: 9736 9736 Size: 9742 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2243 3865 5418 6817 Size: 53 83 186 936 710 1622 1553 1399 Cuts at: 6817 6891 7549 7931 8566 275 Size: 74 658 382 635 1451 Fragments arranged by size: 1622 1553 1451 1399 936 710 658 635 382 186 83 74 53 BsaWI w'CCGG_w Cuts at: 8175 8573 8847 8994 9405 8175 Size: 398 274 147 411 8512 Fragments arranged by size: 8512 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2516 2546 3534 3564 4022 Size: 30 165 30 1113 30 988 30 458 Cuts at: 4022 4052 4216 4246 4452 4482 6170 6200 6363 Size: 30 164 30 206 30 1688 30 163 Cuts at: 6363 6393 1178 Size: 30 4527 Fragments arranged by size: 4527 1688 1113 988 458 206 165 164 163 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2103 2390 2678 4058 4310 4889 5087 5528 Size: 917 287 288 1380 252 579 198 441 Cuts at: 5528 5891 5912 5921 6884 6949 1186 Size: 363 21 9 963 65 3979 Fragments arranged by size: 3979 1380 963 917 579 441 363 288 287 252 198 65 21 9 BseYI C'CCAG_C Cuts at: 2078 2216 2319 2431 2463 2941 3692 3758 3763 Size: 138 103 112 32 478 751 66 5 Cuts at: 3763 3954 4388 4798 5047 5175 5552 6021 7024 Size: 191 434 410 249 128 377 469 1003 Cuts at: 7024 8945 9441 2078 Size: 1921 496 2379 Fragments arranged by size: 2379 1921 1003 751 496 478 469 434 410 377 249 191 138 128 112 103 66 32 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2025 2315 2642 5148 5762 6143 6380 Size: 573 289 290 327 2506 614 381 237 Cuts at: 6380 6431 1163 Size: 51 4474 Fragments arranged by size: 4474 2506 614 573 381 327 290 289 237 51 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6981 7192 7953 8981 1540 Size: 275 186 4980 211 761 1028 2301 Fragments arranged by size: 4980 2301 1028 761 275 211 186 BsiHKAI G_wGCw'C Cuts at: 2093 2367 2556 3507 5807 6237 6243 6405 7803 Size: 274 189 951 2300 430 6 162 1398 Cuts at: 7803 7888 8959 2093 Size: 85 1071 2876 Fragments arranged by size: 2876 2300 1398 1071 951 430 274 189 162 85 6 BsmI GAATG_Cn' Cuts at: 3249 5443 7253 7346 3249 Size: 2194 1810 93 5645 Fragments arranged by size: 5645 2194 1810 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2093 2214 2367 2439 2556 2870 Size: 399 245 58 121 153 72 117 314 Cuts at: 2870 3315 3352 3507 3605 3878 4179 4488 4574 Size: 445 37 155 98 273 301 309 86 Cuts at: 4574 4626 4633 4670 5292 5657 5807 6144 6237 Size: 52 7 37 622 365 150 337 93 Cuts at: 6237 6243 6405 6456 6534 6814 6913 6943 6977 Size: 6 162 51 78 280 99 30 34 Cuts at: 6977 6999 7061 7803 7888 8959 1391 Size: 22 62 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 622 445 399 365 337 314 309 301 280 273 245 162 155 153 150 121 117 99 98 93 86 85 78 72 62 58 52 51 37 37 34 30 22 7 6 BspEI T'CCGG_A Cuts at: 9405 9405 Size: 9742 BspHI T'CATG_A Cuts at: 4089 7631 9361 4089 Size: 3542 1730 4470 Fragments arranged by size: 4470 3542 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5070 7097 844 Size: 4226 2027 3489 Fragments arranged by size: 4226 3489 2027 BsrBI CCG'CTC Cuts at: 1394 1823 4774 5371 7629 1394 Size: 429 2951 597 2258 3507 Fragments arranged by size: 3507 2951 2258 597 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2300 5823 6435 8237 8411 65 Size: 1183 1052 3523 612 1802 174 1396 Fragments arranged by size: 3523 1802 1396 1183 1052 612 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2976 6419 7003 8386 8573 1700 Size: 110 1166 3443 584 1383 187 2869 Fragments arranged by size: 3443 2869 1383 1166 584 187 110 BsrGI T'GTAC_A Cuts at: 96 5609 6129 96 Size: 5513 520 3709 Fragments arranged by size: 5513 3709 520 BssHII G'CGCG_C Cuts at: 1925 5425 6920 1925 Size: 3500 1495 4747 Fragments arranged by size: 4747 3500 1495 BssSI C'ACGA_G Cuts at: 6236 6377 7802 8814 6236 Size: 141 1425 1012 7164 Fragments arranged by size: 7164 1425 1012 141 BstAPI GCAn_nnn'nTGC Cuts at: 794 2302 2629 2920 5171 6034 6409 6726 794 Size: 1508 327 291 2251 863 375 317 3810 Fragments arranged by size: 3810 2251 1508 863 375 327 317 291 BstBI TT'CG_AA Cuts at: 8546 8546 Size: 9742 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9742 BstXI CCAn_nnnn'nTGG Cuts at: 2331 2838 4027 5695 2331 Size: 507 1189 1668 6378 Fragments arranged by size: 6378 1668 1189 507 BstYI r'GATC_y Cuts at: 1972 3492 4062 5076 5307 5767 5787 7072 7439 Size: 1520 570 1014 231 460 20 1285 367 Cuts at: 7439 7824 7841 9293 9379 9391 9737 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1520 1452 1285 1014 570 460 385 367 346 231 86 20 17 12 Bsu36I CC'TnA_GG Cuts at: 2656 3850 4615 2656 Size: 1194 765 7783 Fragments arranged by size: 7783 1194 765 BtgI C'CryG_G Cuts at: 513 1065 2263 3034 3298 4278 5721 6069 513 Size: 552 1198 771 264 980 1443 348 4186 Fragments arranged by size: 4186 1443 1198 980 771 552 348 264 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3542 6744 487 Size: 683 56 2316 3202 3485 Fragments arranged by size: 3485 3202 2316 683 56 BtsI GCAGTG_nn' Cuts at: 2022 2738 4902 6631 6796 7258 8052 8072 2022 Size: 716 2164 1729 165 462 794 20 3692 Fragments arranged by size: 3692 2164 1729 794 716 462 165 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9742 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9707 DraI TTT'AAA Cuts at: 7046 7046 Size: 9742 DraIII CAC_nnn'GTG Cuts at: 5243 5282 5722 6283 6409 5243 Size: 39 440 561 126 8576 Fragments arranged by size: 8576 561 440 126 39 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8749 809 Size: 507 7433 1802 Fragments arranged by size: 7433 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2446 2971 3301 Size: 54 1425 50 275 18 616 525 330 Cuts at: 3301 3726 4080 4281 4395 6308 6978 7189 8078 Size: 425 354 201 114 1913 670 211 889 Cuts at: 8078 9707 8 Size: 1629 43 Fragments arranged by size: 1913 1629 1425 889 670 616 525 425 354 330 275 211 201 114 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6978 7189 1537 Size: 275 5166 211 4090 Fragments arranged by size: 5166 4090 275 211 EarI CTCTTCn'nnn_ Cuts at: 1353 3495 3512 4116 4287 4647 4734 5361 5684 Size: 2142 17 604 171 360 87 627 323 Cuts at: 5684 7672 1353 Size: 1988 3423 Fragments arranged by size: 3423 2142 1988 627 604 360 323 171 87 17 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8329 8703 8849 387 Size: 1064 138 6740 374 146 1280 Fragments arranged by size: 6740 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2091 3505 5805 2091 Size: 1414 2300 6028 Fragments arranged by size: 6028 2300 1414 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2010 2107 2154 2220 2543 2700 2931 Size: 393 378 97 47 66 323 157 231 Cuts at: 2931 3094 3305 3801 3844 4049 4190 4448 4479 Size: 163 211 496 43 205 141 258 31 Cuts at: 4479 5127 5285 5324 5585 6872 7805 8402 9189 Size: 648 158 39 261 1287 933 597 787 Cuts at: 9189 9457 1239 Size: 268 1524 Fragments arranged by size: 1524 1287 933 787 648 597 496 393 378 323 268 261 258 231 211 205 163 158 157 141 97 66 47 43 39 31 EcoNI CCTnn'n_nnAGG Cuts at: 3990 3990 Size: 9742 EcoO109I rG'GnC_Cy Cuts at: 2031 2065 2082 2435 2436 2509 2585 2651 2668 Size: 34 17 353 1 73 76 66 17 Cuts at: 2668 2866 3008 3311 3312 3524 3575 3781 3935 Size: 198 142 303 1 212 51 206 154 Cuts at: 3935 3995 4485 4623 4667 5020 5115 5288 5289 Size: 60 490 138 44 353 95 173 1 Cuts at: 5289 5644 6452 6453 6909 7021 2031 Size: 355 808 1 456 112 4752 Fragments arranged by size: 4752 808 490 456 355 353 353 303 212 206 198 173 154 142 138 112 95 76 73 66 60 51 44 34 17 17 1 1 1 1 EcoRI G'AATT_C Cuts at: 4055 7051 7429 4055 Size: 2996 378 6368 Fragments arranged by size: 6368 2996 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4195 5362 4195 Size: 1167 8575 FspI TGC'GCA Cuts at: 5381 8248 5381 Size: 2867 6875 HaeII r_GCGC'y Cuts at: 1678 2246 2403 2902 3261 3868 4566 6264 6820 Size: 568 157 499 359 607 698 1698 556 Cuts at: 6820 8889 1678 Size: 2069 2531 Fragments arranged by size: 2531 2069 1698 698 607 568 556 499 359 157 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2493 2525 5068 5100 5570 Size: 32 135 32 921 32 2543 32 470 Cuts at: 5570 5602 5764 5796 5885 5917 8383 8415 8457 Size: 32 162 32 89 32 2466 32 42 Cuts at: 8457 8489 1373 Size: 32 2626 Fragments arranged by size: 2626 2543 2466 921 470 162 135 89 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 6193 7086 7332 669 Size: 988 281 4255 893 246 3079 Fragments arranged by size: 4255 3079 988 893 281 246 HindIII A'AGCT_T Cuts at: 748 2419 3856 748 Size: 1671 1437 6634 Fragments arranged by size: 6634 1671 1437 HpaI GTT'AAC Cuts at: 7332 7332 Size: 9742 KasI G'GCGC_C Cuts at: 2242 3864 6816 2242 Size: 1622 2952 5168 Fragments arranged by size: 5168 2952 1622 KpnI G_GTAC'C Cuts at: 7067 7067 Size: 9742 MfeI C'AATT_G Cuts at: 7341 7341 Size: 9742 MluI A'CGCG_T Cuts at: 9618 9618 Size: 9742 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3311 8856 9040 1305 Size: 2006 5545 184 2007 Fragments arranged by size: 5545 2007 2006 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2973 3728 4082 6310 10 Size: 54 1425 343 1141 755 354 2228 3442 Fragments arranged by size: 3442 2228 1425 1141 755 354 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2289 2370 2856 4094 5633 5738 6137 Size: 648 1123 81 486 1238 1539 105 399 Cuts at: 6137 6902 7700 8059 8218 518 Size: 765 798 359 159 2042 Fragments arranged by size: 2042 1539 1238 1123 798 765 648 486 399 359 159 105 81 MspA1I CmG'CkG Cuts at: 1606 2911 3356 4027 4168 4466 4553 4829 4908 Size: 1305 445 671 141 298 87 276 79 Cuts at: 4908 6025 6127 6478 6931 7158 7835 8983 9228 Size: 1117 102 351 453 227 677 1148 245 Cuts at: 9228 1606 Size: 2120 Fragments arranged by size: 2120 1305 1148 1117 677 671 453 445 351 298 276 245 227 141 102 87 79 NaeI GCC'GGC Cuts at: 7005 7005 Size: 9742 NarI GG'CG_CC Cuts at: 2243 3865 6817 2243 Size: 1622 2952 5168 Fragments arranged by size: 5168 2952 1622 NcoI C'CATG_G Cuts at: 513 1065 2263 3034 3298 513 Size: 552 1198 771 264 6957 Fragments arranged by size: 6957 1198 771 552 264 NdeI CA'TA_TG Cuts at: 387 387 Size: 9742 NgoMIV G'CCGG_C Cuts at: 7003 7003 Size: 9742 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9742 NotI GC'GGCC_GC Cuts at: 7189 7189 Size: 9742 NruI TCG'CGA Cuts at: 3561 7039 3561 Size: 3478 6264 NsiI A_TGCA'T Cuts at: 2010 6334 2010 Size: 4324 5418 NspI r_CATG'y Cuts at: 1132 1229 5263 6082 7100 8645 1132 Size: 97 4034 819 1018 1545 2229 Fragments arranged by size: 4034 2229 1545 1018 819 97 PasI CC'CwG_GG Cuts at: 1348 2729 3885 4567 4626 5231 1348 Size: 1381 1156 682 59 605 5859 Fragments arranged by size: 5859 1381 1156 682 605 59 PciI A'CATG_T Cuts at: 8641 8641 Size: 9742 PflMI CCAn_nnn'nTGG Cuts at: 1108 2980 6423 1108 Size: 1872 3443 4427 Fragments arranged by size: 4427 3443 1872 PfoI T'CCnGG_A Cuts at: 2307 5859 6315 2307 Size: 3552 456 5734 Fragments arranged by size: 5734 3552 456 PmeI GTTT'AAAC Cuts at: 7046 7046 Size: 9742 PmlI CAC'GTG Cuts at: 6091 6544 6091 Size: 453 9289 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5794 5826 7811 7843 9324 9356 5794 Size: 32 1985 32 1481 32 6180 Fragments arranged by size: 6180 1985 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2509 2585 3524 3781 5020 2509 Size: 76 939 257 1239 7231 Fragments arranged by size: 7231 1239 939 257 76 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9742 PsiI TTA'TAA Cuts at: 7312 7312 Size: 9742 PspOMI G'GGCC_C Cuts at: 2031 2435 2866 3311 4484 4622 4629 4666 5288 Size: 404 431 445 1173 138 7 37 622 Cuts at: 5288 6452 6909 2031 Size: 1164 457 4864 Fragments arranged by size: 4864 1173 1164 622 457 445 431 404 138 37 7 PstI C_TGCA'G Cuts at: 830 2457 2641 4194 5067 5604 5874 6030 6621 Size: 1627 184 1553 873 537 270 156 591 Cuts at: 6621 6837 6958 7094 830 Size: 216 121 136 3478 Fragments arranged by size: 3478 1627 1553 873 591 537 270 216 184 156 136 121 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9742 PvuII CAG'CTG Cuts at: 4466 4908 6025 6127 6478 4466 Size: 442 1117 102 351 7730 Fragments arranged by size: 7730 1117 442 351 102 SacI G_AGCT'C Cuts at: 2093 3507 5807 2093 Size: 1414 2300 6028 Fragments arranged by size: 6028 2300 1414 SalI G'TCGA_C Cuts at: 1936 7084 1936 Size: 5148 4594 SanDI GG'GwC_CC Cuts at: 3524 3524 Size: 9742 SapI GCTCTTCn'nnn_ Cuts at: 3495 3512 4647 5361 3495 Size: 17 1135 714 7876 Fragments arranged by size: 7876 1135 714 17 SbfI CC_TGCA'GG Cuts at: 6837 7094 6837 Size: 257 9485 ScaI AGT'ACT Cuts at: 4442 7990 9702 4442 Size: 3548 1712 4482 Fragments arranged by size: 4482 3548 1712 SexAI A'CCwGG_T Cuts at: 6789 6789 Size: 9742 SfcI C'TryA_G Cuts at: 826 1045 2453 2637 4190 5063 5600 5870 6026 Size: 219 1408 184 1553 873 537 270 156 Cuts at: 6026 6617 6833 6954 7090 8225 8906 9097 826 Size: 591 216 121 136 1135 681 191 1471 Fragments arranged by size: 1553 1471 1408 1135 873 681 591 537 270 219 216 191 184 156 136 121 SfiI GGCCn_nnn'nGGCC Cuts at: 5123 5123 Size: 9742 SfoI GGC'GCC Cuts at: 2244 3866 6818 2244 Size: 1622 2952 5168 Fragments arranged by size: 5168 2952 1622 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9742 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9742 SmaI CCC'GGG Cuts at: 1872 3970 4634 4665 5528 7069 1872 Size: 2098 664 31 863 1541 4545 Fragments arranged by size: 4545 2098 1541 863 664 31 SmlI C'TyrA_G Cuts at: 820 1017 1943 2549 7846 8747 9009 820 Size: 197 926 606 5297 901 262 1553 Fragments arranged by size: 5297 1553 926 901 606 262 197 SnaBI TAC'GTA Cuts at: 493 7533 493 Size: 7040 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9742 SphI G_CATG'C Cuts at: 1132 7100 1132 Size: 5968 3774 SspI AAT'ATT Cuts at: 5 52 6340 7666 5 Size: 47 6288 1326 2081 Fragments arranged by size: 6288 2081 1326 47 StuI AGG'CCT Cuts at: 1517 5849 6831 6884 1517 Size: 4332 982 53 4375 Fragments arranged by size: 4375 4332 982 53 StyI C'CwwG_G Cuts at: 513 1065 2059 2263 2505 3034 3298 4413 4869 Size: 552 994 204 242 529 264 1115 456 Cuts at: 4869 5010 5016 5121 5295 5442 7181 513 Size: 141 6 105 174 147 1739 3074 Fragments arranged by size: 3074 1739 1115 994 552 529 456 264 242 204 174 147 141 105 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2943 4301 4697 5149 5566 5750 6397 Size: 1255 1363 1358 396 452 417 184 647 Cuts at: 6397 7769 7786 7939 8124 8569 325 Size: 1372 17 153 185 445 1498 Fragments arranged by size: 1498 1372 1363 1358 1255 647 452 445 417 396 185 184 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4440 5609 6129 Size: 274 80 33 51 1443 2463 1169 520 Cuts at: 6129 7988 9700 96 Size: 1859 1712 138 Fragments arranged by size: 2463 1859 1712 1443 1169 520 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2516 2548 3534 3566 4216 4248 6363 6395 9702 Size: 32 986 32 650 32 2115 32 3307 Cuts at: 9702 9734 2516 Size: 32 2524 Fragments arranged by size: 3307 2524 2115 986 650 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9742 XbaI T'CTAG_A Cuts at: 7078 7078 Size: 9742 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3444 5560 5692 5695 3444 Size: 2116 132 3 7491 Fragments arranged by size: 7491 2116 132 3 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9742 XmaI C'CCGG_G Cuts at: 1870 3968 4632 4663 5526 7067 1870 Size: 2098 664 31 863 1541 4545 Fragments arranged by size: 4545 2098 1541 863 664 31 XmnI GAAnn'nnTTC Cuts at: 3338 4110 7871 3338 Size: 772 3761 5209 Fragments arranged by size: 5209 3761 772 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5419 7550 276 Size: 53 83 186 936 3885 2131 2468 Fragments arranged by size: 3885 2468 2131 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BplI BsiWI BsmBI BstZ17I EcoRV FseI FspAI PacI PspXI PsrI RsrII SacII SrfI SwaI