(Circular) (Six-base) MAPSORT of: pFN21ASDA0341.seq Check: 8534 from: 1 to: 6361 pFN21ASDA0341 6361 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2863 2863 Size: 6361 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4171 278 Size: 53 83 186 936 2635 2468 Fragments arranged by size: 2635 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3704 1937 Size: 1767 4594 Acc65I G'GTAC_C Cuts at: 3682 3682 Size: 6361 AclI AA'CG_TT Cuts at: 1563 4488 4861 1563 Size: 2925 373 3063 Fragments arranged by size: 3063 2925 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2121 2296 2629 2933 3202 3245 4424 5808 Size: 489 175 333 304 269 43 1179 1384 Cuts at: 5808 6076 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1179 489 333 304 269 268 175 43 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6164 AflIII A'CryG_T Cuts at: 1203 3561 5260 6237 1203 Size: 2358 1699 977 1327 Fragments arranged by size: 2358 1699 1327 977 AgeI A'CCGG_T Cuts at: 5192 5192 Size: 6361 AhdI GACnn_n'nnGTC Cuts at: 5090 5090 Size: 6361 AleI CACnn'nnGTG Cuts at: 1166 2873 1166 Size: 1707 4654 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3047 3392 1184 Size: 1863 345 4153 Fragments arranged by size: 4153 1863 345 AlwNI CAG_nnn'CTG Cuts at: 2042 2446 2536 2878 2896 3049 3089 3172 3205 Size: 404 90 342 18 153 40 83 33 Cuts at: 3205 3472 3583 3595 4104 5676 2042 Size: 267 111 12 509 1572 2727 Fragments arranged by size: 2727 1572 509 404 342 267 153 111 90 83 40 33 18 12 ApaI G_GGCC'C Cuts at: 2248 2508 3070 2248 Size: 260 562 5539 Fragments arranged by size: 5539 562 260 ApaLI G'TGCA_C Cuts at: 3099 4418 5574 3099 Size: 1319 1156 3886 Fragments arranged by size: 3886 1319 1156 ApoI r'AATT_y Cuts at: 1493 3670 3896 4048 6157 1493 Size: 2177 226 152 2109 1697 Fragments arranged by size: 2177 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4915 160 Size: 872 3883 1606 Fragments arranged by size: 3883 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6361 AvaI C'yCGr_G Cuts at: 1870 1943 2130 2501 2508 2523 2573 2675 2747 Size: 73 187 371 7 15 50 102 72 Cuts at: 2747 2810 3063 3324 3638 3686 1870 Size: 63 253 261 314 48 4545 Fragments arranged by size: 4545 371 314 261 253 187 102 73 72 63 50 48 15 7 BamHI G'GATC_C Cuts at: 3691 4058 3691 Size: 367 5994 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3015 3074 3682 5137 618 Size: 325 217 627 1228 59 608 1455 1842 Fragments arranged by size: 1842 1455 1228 627 608 325 217 59 BanII G_rGCy'C Cuts at: 1391 2138 2157 2248 2501 2508 2574 2580 2684 Size: 747 19 91 253 7 66 6 104 Cuts at: 2684 2829 3040 3070 3615 3680 1391 Size: 145 211 30 545 65 4072 Fragments arranged by size: 4072 747 545 253 211 145 104 91 66 65 30 19 7 6 BbeI G_GCGC'C Cuts at: 3019 3019 Size: 6361 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3146 3233 928 Size: 971 1247 87 4056 Fragments arranged by size: 4056 1247 971 87 BbvCI CC'TCA_GC Cuts at: 2189 2546 2189 Size: 357 6004 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3305 3339 4552 4586 1903 Size: 34 1368 34 1213 34 3678 Fragments arranged by size: 3678 1368 1213 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4253 5463 4253 Size: 1210 5151 BclI T'GATC_A Cuts at: 1548 1548 Size: 6361 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2046 2152 2519 Size: 107 122 71 631 605 374 106 367 Cuts at: 2519 4972 136 Size: 2453 1525 Fragments arranged by size: 2453 1525 631 605 374 367 122 107 106 71 BglII A'GATC_T Cuts at: 3213 3651 6356 3213 Size: 438 2705 3218 Fragments arranged by size: 3218 2705 438 BlpI GC'TnA_GC Cuts at: 2735 2907 2958 3000 3039 3417 3432 3778 2735 Size: 172 51 42 39 378 15 346 5318 Fragments arranged by size: 5318 378 346 172 51 42 39 15 Bme1580I G_kGCm'C Cuts at: 1790 2248 2508 2530 3070 3103 3253 3604 4422 Size: 458 260 22 540 33 150 351 818 Cuts at: 4422 5578 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 818 540 458 351 260 150 33 22 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6361 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3090 4092 5050 Size: 416 53 83 399 24 1673 1002 958 Cuts at: 5050 442 Size: 1753 Fragments arranged by size: 1753 1673 1002 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6361 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2872 2872 Size: 6361 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2581 2900 2963 3605 3646 5021 1239 Size: 1342 319 63 642 41 1375 2579 Fragments arranged by size: 2579 1375 1342 642 319 63 41 Bpu10I CC'TnA_GC Cuts at: 2189 2495 2546 2568 3264 2189 Size: 306 51 22 696 5286 Fragments arranged by size: 5286 696 306 51 22 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2685 2813 4486 5351 5649 2685 Size: 128 1673 865 298 3397 Fragments arranged by size: 3397 1673 865 298 128 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2133 2807 5024 882 Size: 617 634 674 2217 2219 Fragments arranged by size: 2219 2217 674 634 617 BsaAI yAC'GTr Cuts at: 493 1204 3658 4152 493 Size: 711 2454 494 2702 Fragments arranged by size: 2702 2454 711 494 BsaBI GATnn'nnATC Cuts at: 6355 6355 Size: 6361 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2426 3016 4168 4550 Size: 53 83 186 936 893 590 1152 382 Cuts at: 4550 5185 275 Size: 635 1451 Fragments arranged by size: 1451 1152 936 893 635 590 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2160 3055 4794 5192 5466 5613 6024 2160 Size: 895 1739 398 274 147 411 2497 Fragments arranged by size: 2497 1739 895 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2756 2786 1178 Size: 30 165 30 1353 30 4753 Fragments arranged by size: 4753 1353 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2147 2584 2641 2770 2837 2897 3125 3521 Size: 961 437 57 129 67 60 228 396 Cuts at: 3521 1186 Size: 4026 Fragments arranged by size: 4026 961 437 396 228 129 67 60 57 BseYI C'CCAG_C Cuts at: 2579 2717 5564 6060 2579 Size: 138 2847 496 2880 Fragments arranged by size: 2880 2847 496 138 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2183 2453 2522 2921 1163 Size: 573 447 270 69 399 4603 Fragments arranged by size: 4603 573 447 399 270 69 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3811 4572 5600 1540 Size: 275 186 1810 761 1028 2301 Fragments arranged by size: 2301 1810 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2228 2829 3103 4422 4507 5578 2228 Size: 601 274 1319 85 1071 3011 Fragments arranged by size: 3011 1319 1071 601 274 85 BsmI GAATG_Cn' Cuts at: 2358 3872 3965 2358 Size: 1514 93 4754 Fragments arranged by size: 4754 1514 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2138 2157 2228 2248 2501 2508 2530 Size: 399 348 19 71 20 253 7 22 Cuts at: 2530 2574 2580 2684 2829 3040 3070 3103 3253 Size: 44 6 104 145 211 30 33 150 Cuts at: 3253 3604 3615 3680 4422 4507 5578 1391 Size: 351 11 65 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 351 348 253 211 150 145 104 85 71 65 44 33 30 22 20 19 11 7 6 BspEI T'CCGG_A Cuts at: 2160 3055 6024 2160 Size: 895 2969 2497 Fragments arranged by size: 2969 2497 895 BspHI T'CATG_A Cuts at: 4250 5980 4250 Size: 1730 4631 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2205 2863 3716 844 Size: 1361 658 853 3489 Fragments arranged by size: 3489 1361 853 658 BsrBI CCG'CTC Cuts at: 1394 1823 2932 3490 4248 1394 Size: 429 1109 558 758 3507 Fragments arranged by size: 3507 1109 758 558 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2049 2464 4856 5030 65 Size: 1183 801 415 2392 174 1396 Fragments arranged by size: 2392 1396 1183 801 415 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5005 5192 1700 Size: 110 3195 187 2869 Fragments arranged by size: 3195 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6361 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6361 BssSI C'ACGA_G Cuts at: 2939 4421 5433 2939 Size: 1482 1012 3867 Fragments arranged by size: 3867 1482 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3172 3472 3595 794 Size: 2378 300 123 3560 Fragments arranged by size: 3560 2378 300 123 BstBI TT'CG_AA Cuts at: 2007 5165 2007 Size: 3158 3203 BstEII G'GTnAC_C Cuts at: 1184 2944 1184 Size: 1760 4601 BstXI CCAn_nnnn'nTGG Cuts at: 2150 2150 Size: 6361 BstYI r'GATC_y Cuts at: 1972 3213 3540 3651 3691 4058 4443 4460 5912 Size: 1241 327 111 40 367 385 17 1452 Cuts at: 5912 5998 6010 6356 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1241 385 367 346 327 111 86 40 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2055 2108 513 Size: 552 957 33 53 4766 Fragments arranged by size: 4766 957 552 53 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2859 487 Size: 683 56 1633 3989 Fragments arranged by size: 3989 1633 683 56 BtsI GCAGTG_nn' Cuts at: 3602 3877 4671 4691 3602 Size: 275 794 20 5272 Fragments arranged by size: 5272 794 275 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6361 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6326 DraI TTT'AAA Cuts at: 3665 3665 Size: 6361 DraIII CAC_nnn'GTG Cuts at: 2944 2944 Size: 6361 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5368 809 Size: 507 4052 1802 Fragments arranged by size: 4052 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2659 3808 Size: 54 1425 50 275 18 195 634 1149 Cuts at: 3808 4697 6326 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1149 889 634 275 195 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3808 1537 Size: 275 1996 4090 Fragments arranged by size: 4090 1996 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2097 4291 1353 Size: 744 2194 3423 Fragments arranged by size: 3423 2194 744 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3203 3224 3508 4948 5322 5468 Size: 1064 138 1614 21 284 1440 374 146 Cuts at: 5468 387 Size: 1280 Fragments arranged by size: 1614 1440 1280 1064 374 284 146 138 21 EcoICRI GAG'CTC Cuts at: 2827 2827 Size: 6361 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2121 2296 2581 2629 2900 2933 2963 Size: 393 489 175 285 48 271 33 30 Cuts at: 2963 3202 3245 3605 3646 4424 5021 5808 6076 Size: 239 43 360 41 778 597 787 268 Cuts at: 6076 1239 Size: 1524 Fragments arranged by size: 1524 787 778 597 489 393 360 285 271 268 239 175 48 43 41 33 30 EcoNI CCTnn'n_nnAGG Cuts at: 2162 2555 3023 3083 2162 Size: 393 468 60 5440 Fragments arranged by size: 5440 468 393 60 EcoO109I rG'GnC_Cy Cuts at: 2039 2245 2505 2705 2836 3046 3067 3319 3373 Size: 206 260 200 131 210 21 252 54 Cuts at: 3373 2039 Size: 5027 Fragments arranged by size: 5027 260 252 210 206 200 131 54 21 EcoRI G'AATT_C Cuts at: 3670 4048 3670 Size: 378 5983 FspI TGC'GCA Cuts at: 3023 3302 4867 3023 Size: 279 1565 4517 Fragments arranged by size: 4517 1565 279 HaeII r_GCGC'y Cuts at: 1678 2890 2920 3019 3473 5508 1678 Size: 1212 30 99 454 2035 2531 Fragments arranged by size: 2531 2035 1212 454 99 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2681 2713 3205 3237 5002 Size: 32 135 32 1109 32 492 32 1765 Cuts at: 5002 5034 5076 5108 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1765 1109 492 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3705 3951 669 Size: 988 281 1767 246 3079 Fragments arranged by size: 3079 1767 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6361 HpaI GTT'AAC Cuts at: 3951 3951 Size: 6361 KasI G'GCGC_C Cuts at: 3015 3015 Size: 6361 KpnI G_GTAC'C Cuts at: 3686 3686 Size: 6361 MfeI C'AATT_G Cuts at: 3960 3960 Size: 6361 MluI A'CGCG_T Cuts at: 6237 6237 Size: 6361 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2058 2613 2883 5475 5659 1305 Size: 753 555 270 2592 184 2007 Fragments arranged by size: 2592 2007 753 555 270 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2027 2661 10 Size: 54 1425 343 195 634 3710 Fragments arranged by size: 3710 1425 634 343 195 54 MslI CAynn'nnrTG Cuts at: 518 1166 2054 2873 3560 4319 4678 4837 518 Size: 648 888 819 687 759 359 159 2042 Fragments arranged by size: 2042 888 819 759 687 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2110 2533 2729 2893 2983 2989 3013 3172 Size: 504 423 196 164 90 6 24 159 Cuts at: 3172 3345 3424 3535 3777 4454 5602 5847 1606 Size: 173 79 111 242 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 504 423 245 242 196 173 164 159 111 90 79 24 6 NarI GG'CG_CC Cuts at: 3016 3016 Size: 6361 NcoI C'CATG_G Cuts at: 513 1065 2022 2055 513 Size: 552 957 33 4819 Fragments arranged by size: 4819 957 552 33 NdeI CA'TA_TG Cuts at: 387 387 Size: 6361 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6361 NotI GC'GGCC_GC Cuts at: 3808 3808 Size: 6361 NspI r_CATG'y Cuts at: 1132 1229 3565 3719 5264 1132 Size: 97 2336 154 1545 2229 Fragments arranged by size: 2336 2229 1545 154 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6361 PciI A'CATG_T Cuts at: 3561 5260 3561 Size: 1699 4662 PflMI CCAn_nnn'nTGG Cuts at: 1108 3205 3583 3624 1108 Size: 2097 378 41 3845 Fragments arranged by size: 3845 2097 378 41 PmeI GTTT'AAAC Cuts at: 3665 3665 Size: 6361 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4430 4462 5943 5975 4430 Size: 32 1481 32 4816 Fragments arranged by size: 4816 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2705 2705 Size: 6361 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6361 PsiI TTA'TAA Cuts at: 3931 3931 Size: 6361 PspOMI G'GGCC_C Cuts at: 2244 2504 3066 2244 Size: 260 562 5539 Fragments arranged by size: 5539 562 260 PspXI vC'TCGA_Gb Cuts at: 3638 3638 Size: 6361 PstI C_TGCA'G Cuts at: 830 2988 3312 3444 3713 830 Size: 2158 324 132 269 3478 Fragments arranged by size: 3478 2158 324 269 132 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6361 PvuII CAG'CTG Cuts at: 2533 2729 2983 2989 3172 3345 3424 2533 Size: 196 254 6 183 173 79 5470 Fragments arranged by size: 5470 254 196 183 173 79 6 SacI G_AGCT'C Cuts at: 2829 2829 Size: 6361 SacII CC_GC'GG Cuts at: 2111 2111 Size: 6361 SalI G'TCGA_C Cuts at: 1936 3703 1936 Size: 1767 4594 SapI GCTCTTCn'nnn_ Cuts at: 2097 2097 Size: 6361 SbfI CC_TGCA'GG Cuts at: 3312 3713 3312 Size: 401 5960 ScaI AGT'ACT Cuts at: 4609 6321 4609 Size: 1712 4649 SfcI C'TryA_G Cuts at: 826 1045 2984 3308 3440 3709 4844 5525 5716 Size: 219 1939 324 132 269 1135 681 191 Cuts at: 5716 826 Size: 1471 Fragments arranged by size: 1939 1471 1135 681 324 269 219 191 132 SfiI GGCCn_nnn'nGGCC Cuts at: 2519 2519 Size: 6361 SfoI GGC'GCC Cuts at: 3017 3017 Size: 6361 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6361 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6361 SmaI CCC'GGG Cuts at: 1872 2503 2510 2525 3688 1872 Size: 631 7 15 1163 4545 Fragments arranged by size: 4545 1163 631 15 7 SmlI C'TyrA_G Cuts at: 820 1017 1943 2700 2828 3638 4465 5366 5628 Size: 197 926 757 128 810 827 901 262 Cuts at: 5628 820 Size: 1553 Fragments arranged by size: 1553 926 901 827 810 757 262 197 128 SnaBI TAC'GTA Cuts at: 493 3658 4152 493 Size: 3165 494 2702 Fragments arranged by size: 3165 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6361 SphI G_CATG'C Cuts at: 1132 3719 1132 Size: 2587 3774 SrfI GCCC'GGGC Cuts at: 2525 2525 Size: 6361 SspI AAT'ATT Cuts at: 5 52 4285 5 Size: 47 4233 2081 Fragments arranged by size: 4233 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6361 StyI C'CwwG_G Cuts at: 513 1065 2022 2055 2656 2709 2839 3800 513 Size: 552 957 33 601 53 130 961 3074 Fragments arranged by size: 3074 961 957 601 552 130 53 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4388 4405 4558 4743 5188 325 Size: 1255 2808 17 153 185 445 1498 Fragments arranged by size: 2808 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4607 6319 96 Size: 274 80 33 51 1443 2630 1712 138 Fragments arranged by size: 2630 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6321 6353 6321 Size: 32 6329 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6361 XbaI T'CTAG_A Cuts at: 3697 3697 Size: 6361 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2062 2419 2062 Size: 357 6004 XhoI C'TCGA_G Cuts at: 1943 3638 1943 Size: 1695 4666 XmaI C'CCGG_G Cuts at: 1870 2501 2508 2523 3686 1870 Size: 631 7 15 1163 4545 Fragments arranged by size: 4545 1163 631 15 7 XmnI GAAnn'nnTTC Cuts at: 2402 4490 2402 Size: 2088 4273 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4169 276 Size: 53 83 186 936 2635 2468 Fragments arranged by size: 2635 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PsrI RsrII SanDI SexAI SwaI