(Circular) (Six-base) MAPSORT of: pFN21ASDA0334.seq Check: 7769 from: 1 to: 7267 pFN21ASDA0334 7267 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5077 278 Size: 53 83 186 936 3541 2468 Fragments arranged by size: 3541 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4610 1937 Size: 2673 4594 Acc65I G'GTAC_C Cuts at: 4195 4588 4195 Size: 393 6874 AclI AA'CG_TT Cuts at: 1563 5394 5767 1563 Size: 3831 373 3063 Fragments arranged by size: 3831 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2688 3059 3671 4311 5330 6714 6982 1632 Size: 1056 371 612 640 1019 1384 268 1917 Fragments arranged by size: 1917 1384 1056 1019 640 612 371 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7070 AflIII A'CryG_T Cuts at: 1203 6166 7143 1203 Size: 4963 977 1327 Fragments arranged by size: 4963 1327 977 AgeI A'CCGG_T Cuts at: 6098 6098 Size: 7267 AhdI GACnn_n'nnGTC Cuts at: 5996 5996 Size: 7267 AleI CACnn'nnGTG Cuts at: 1166 4016 1166 Size: 2850 4417 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7267 AlwNI CAG_nnn'CTG Cuts at: 3221 3709 5010 6582 3221 Size: 488 1301 1572 3906 Fragments arranged by size: 3906 1572 1301 488 ApaLI G'TGCA_C Cuts at: 2821 5324 6480 2821 Size: 2503 1156 3608 Fragments arranged by size: 3608 2503 1156 ApoI r'AATT_y Cuts at: 1493 2307 2597 2663 2674 2980 3109 3478 3532 Size: 814 290 66 11 306 129 369 54 Cuts at: 3532 3672 3745 3775 4576 4802 4954 7063 1493 Size: 140 73 30 801 226 152 2109 1697 Fragments arranged by size: 2109 1697 814 801 369 306 290 226 152 140 129 73 66 54 30 11 AseI AT'TA_AT Cuts at: 160 1032 2360 5821 160 Size: 872 1328 3461 1606 Fragments arranged by size: 3461 1606 1328 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7267 AvaI C'yCGr_G Cuts at: 1870 1943 4592 1870 Size: 73 2649 4545 Fragments arranged by size: 4545 2649 73 BamHI G'GATC_C Cuts at: 2195 4597 4964 2195 Size: 2402 367 4498 Fragments arranged by size: 4498 2402 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2899 4195 4588 6043 618 Size: 325 217 627 1112 1296 393 1455 1842 Fragments arranged by size: 1842 1455 1296 1112 627 393 325 217 BanII G_rGCy'C Cuts at: 1391 2057 4307 4586 1391 Size: 666 2250 279 4072 Fragments arranged by size: 4072 2250 666 279 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6296 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5458 5492 1903 Size: 34 3521 34 3678 Fragments arranged by size: 3678 3521 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2707 3388 5159 6369 2707 Size: 681 1771 1210 3605 Fragments arranged by size: 3605 1771 1210 681 BclI T'GATC_A Cuts at: 1548 3295 1548 Size: 1747 5520 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5878 136 Size: 107 122 71 631 605 4206 1525 Fragments arranged by size: 4206 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7262 7262 Size: 7267 BlpI GC'TnA_GC Cuts at: 4350 4684 4350 Size: 334 6933 Bme1580I G_kGCm'C Cuts at: 1790 2825 2902 3951 5328 6484 1790 Size: 1035 77 1049 1377 1156 2573 Fragments arranged by size: 2573 1377 1156 1049 1035 77 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7267 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2201 3478 3553 Size: 416 53 83 399 24 784 1277 75 Cuts at: 3553 3966 4998 5956 442 Size: 413 1032 958 1753 Fragments arranged by size: 1753 1277 1032 958 784 416 413 399 83 75 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7267 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5927 1239 Size: 4688 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3257 3507 4119 5392 6257 6555 3257 Size: 250 612 1273 865 298 3969 Fragments arranged by size: 3969 1273 865 612 298 250 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5930 882 Size: 617 4431 2219 Fragments arranged by size: 4431 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4564 5058 493 Size: 711 3360 494 2702 Fragments arranged by size: 3360 2702 711 494 BsaBI GATnn'nnATC Cuts at: 2958 7261 2958 Size: 4303 2964 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5074 5456 6091 275 Size: 53 83 186 936 3541 382 635 1451 Fragments arranged by size: 3541 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5700 6098 6372 6519 6930 5700 Size: 398 274 147 411 6037 Fragments arranged by size: 6037 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3388 3418 1178 Size: 30 165 30 1985 30 5027 Fragments arranged by size: 5027 1985 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7267 BseYI C'CCAG_C Cuts at: 4359 6470 6966 4359 Size: 2111 496 4660 Fragments arranged by size: 4660 2111 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2271 1163 Size: 573 535 6159 Fragments arranged by size: 6159 573 535 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3354 4717 5478 6506 1540 Size: 275 186 1353 1363 761 1028 2301 Fragments arranged by size: 2301 1363 1353 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2057 2825 3003 3404 3989 4307 4457 5328 5413 Size: 768 178 401 585 318 150 871 85 Cuts at: 5413 6484 2057 Size: 1071 2840 Fragments arranged by size: 2840 1071 871 768 585 401 318 178 150 85 BsmI GAATG_Cn' Cuts at: 4778 4871 4778 Size: 93 7174 BsmBI CGTCTCn'nnnn_ Cuts at: 3361 3361 Size: 7267 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2825 2902 3003 3404 3951 3989 Size: 399 267 768 77 101 401 547 38 Cuts at: 3989 4307 4457 4586 5328 5413 6484 1391 Size: 318 150 129 742 85 1071 2174 Fragments arranged by size: 2174 1071 768 742 547 401 399 318 267 150 129 101 85 77 38 BspEI T'CCGG_A Cuts at: 6930 6930 Size: 7267 BspHI T'CATG_A Cuts at: 2397 2995 3430 5156 6886 2397 Size: 598 435 1726 1730 2778 Fragments arranged by size: 2778 1730 1726 598 435 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4622 844 Size: 3778 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5154 1394 Size: 429 3331 3507 Fragments arranged by size: 3507 3331 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5762 5936 65 Size: 1183 4514 174 1396 Fragments arranged by size: 4514 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4512 5911 6098 1700 Size: 110 2702 1399 187 2869 Fragments arranged by size: 2869 2702 1399 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7267 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7267 BssSI C'ACGA_G Cuts at: 5327 6339 5327 Size: 1012 6255 BstAPI GCAn_nnn'nTGC Cuts at: 794 3007 794 Size: 2213 5054 BstBI TT'CG_AA Cuts at: 2007 6071 2007 Size: 4064 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7267 BstXI CCAn_nnnn'nTGG Cuts at: 3239 3491 3239 Size: 252 7015 BstYI r'GATC_y Cuts at: 1972 2195 3384 4597 4964 5349 5366 6818 6904 Size: 223 1189 1213 367 385 17 1452 86 Cuts at: 6904 6916 7262 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1213 1189 385 367 346 223 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3697 4399 513 Size: 552 2632 702 3381 Fragments arranged by size: 3381 2632 702 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6528 Fragments arranged by size: 6528 683 56 BtsI GCAGTG_nn' Cuts at: 2283 4783 5577 5597 2283 Size: 2500 794 20 3953 Fragments arranged by size: 3953 2500 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7267 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7232 DraI TTT'AAA Cuts at: 2577 2688 4571 2577 Size: 111 1883 5273 Fragments arranged by size: 5273 1883 111 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6274 809 Size: 507 4958 1802 Fragments arranged by size: 4958 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3351 4714 5603 Size: 54 1425 50 275 18 1521 1363 889 Cuts at: 5603 7232 8 Size: 1629 43 Fragments arranged by size: 1629 1521 1425 1363 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3351 4714 1537 Size: 275 1539 1363 4090 Fragments arranged by size: 4090 1539 1363 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2340 2707 2838 3192 3210 3325 5197 1353 Size: 987 367 131 354 18 115 1872 3423 Fragments arranged by size: 3423 1872 987 367 354 131 115 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5854 6228 6374 387 Size: 1064 138 4265 374 146 1280 Fragments arranged by size: 4265 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2055 4305 2055 Size: 2250 5017 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2688 3059 3671 4311 5330 5927 6714 Size: 393 1056 371 612 640 1019 597 787 Cuts at: 6714 6982 1239 Size: 268 1524 Fragments arranged by size: 1524 1056 1019 787 640 612 597 393 371 268 EcoNI CCTnn'n_nnAGG Cuts at: 3050 3050 Size: 7267 EcoRI G'AATT_C Cuts at: 2597 3109 4576 4954 2597 Size: 512 1467 378 4910 Fragments arranged by size: 4910 1467 512 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3221 3701 3221 Size: 480 6787 FspI TGC'GCA Cuts at: 5773 5773 Size: 7267 HaeII r_GCGC'y Cuts at: 1678 6414 1678 Size: 4736 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2837 2869 3227 3259 3353 Size: 32 135 32 1265 32 358 32 94 Cuts at: 3353 3385 4219 4251 5908 5940 5982 6014 1373 Size: 32 834 32 1657 32 42 32 2626 Fragments arranged by size: 2626 1657 1265 834 358 135 94 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2065 3929 4142 4611 4857 669 Size: 988 281 127 1864 213 469 246 3079 Fragments arranged by size: 3079 1864 988 469 281 246 213 127 HindIII A'AGCT_T Cuts at: 748 748 Size: 7267 HpaI GTT'AAC Cuts at: 4857 4857 Size: 7267 KpnI G_GTAC'C Cuts at: 4199 4592 4199 Size: 393 6874 MfeI C'AATT_G Cuts at: 3608 4866 3608 Size: 1258 6009 MluI A'CGCG_T Cuts at: 7143 7143 Size: 7267 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6381 6565 1305 Size: 5076 184 2007 Fragments arranged by size: 5076 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5445 Fragments arranged by size: 5445 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2967 4016 5225 5584 5743 518 Size: 648 1801 1049 1209 359 159 2042 Fragments arranged by size: 2042 1801 1209 1049 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3218 4354 4683 5360 6508 6753 1606 Size: 1612 1136 329 677 1148 245 2120 Fragments arranged by size: 2120 1612 1148 1136 677 329 245 NaeI GCC'GGC Cuts at: 4514 4514 Size: 7267 NcoI C'CATG_G Cuts at: 513 1065 3697 4399 513 Size: 552 2632 702 3381 Fragments arranged by size: 3381 2632 702 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7267 NgoMIV G'CCGG_C Cuts at: 4512 4512 Size: 7267 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7267 NotI GC'GGCC_GC Cuts at: 4714 4714 Size: 7267 NspI r_CATG'y Cuts at: 1132 1229 2614 3556 4625 6170 1132 Size: 97 1385 942 1069 1545 2229 Fragments arranged by size: 2229 1545 1385 1069 942 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7267 PciI A'CATG_T Cuts at: 6166 6166 Size: 7267 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7267 PmeI GTTT'AAAC Cuts at: 4571 4571 Size: 7267 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5336 5368 6849 6881 5336 Size: 32 1481 32 5722 Fragments arranged by size: 5722 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7267 PsiI TTA'TAA Cuts at: 2523 4837 2523 Size: 2314 4953 PstI C_TGCA'G Cuts at: 830 2247 3714 4036 4096 4619 830 Size: 1417 1467 322 60 523 3478 Fragments arranged by size: 3478 1467 1417 523 322 60 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7267 PvuII CAG'CTG Cuts at: 3218 4354 3218 Size: 1136 6131 SacI G_AGCT'C Cuts at: 2057 4307 2057 Size: 2250 5017 SalI G'TCGA_C Cuts at: 1936 4609 1936 Size: 2673 4594 SbfI CC_TGCA'GG Cuts at: 4619 4619 Size: 7267 ScaI AGT'ACT Cuts at: 4204 5515 7227 4204 Size: 1311 1712 4244 Fragments arranged by size: 4244 1712 1311 SfcI C'TryA_G Cuts at: 826 1045 2041 2243 3144 3710 4032 4092 4173 Size: 219 996 202 901 566 322 60 81 Cuts at: 4173 4615 5750 6431 6622 826 Size: 442 1135 681 191 1471 Fragments arranged by size: 1471 1135 996 901 681 566 442 322 219 202 191 81 60 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7267 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7267 SmaI CCC'GGG Cuts at: 1872 4594 1872 Size: 2722 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3272 3522 4134 5371 6272 6534 Size: 197 926 1329 250 612 1237 901 262 Cuts at: 6534 820 Size: 1553 Fragments arranged by size: 1553 1329 1237 926 901 612 262 250 197 SnaBI TAC'GTA Cuts at: 493 4564 5058 493 Size: 4071 494 2702 Fragments arranged by size: 4071 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7267 SphI G_CATG'C Cuts at: 1132 4625 1132 Size: 3493 3774 SspI AAT'ATT Cuts at: 5 52 2573 3643 5191 5 Size: 47 2521 1070 1548 2081 Fragments arranged by size: 2521 2081 1548 1070 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7267 StyI C'CwwG_G Cuts at: 513 1065 3697 4399 4545 4706 513 Size: 552 2632 702 146 161 3074 Fragments arranged by size: 3074 2632 702 552 161 146 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2920 3424 4097 5294 5311 5464 5649 Size: 1255 1340 504 673 1197 17 153 185 Cuts at: 5649 6094 325 Size: 445 1498 Fragments arranged by size: 1498 1340 1255 1197 673 504 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3134 3911 3937 Size: 274 80 33 51 1443 1157 777 26 Cuts at: 3937 4202 5513 7225 96 Size: 265 1311 1712 138 Fragments arranged by size: 1712 1443 1311 1157 777 274 265 138 80 51 33 26 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7227 7259 7227 Size: 32 7235 Tth111I GACn'n_nGTC Cuts at: 1316 4273 1316 Size: 2957 4310 XbaI T'CTAG_A Cuts at: 2135 2855 2887 4603 2135 Size: 720 32 1716 4799 Fragments arranged by size: 4799 1716 720 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3845 3845 Size: 7267 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7267 XmaI C'CCGG_G Cuts at: 1870 4592 1870 Size: 2722 4545 XmnI GAAnn'nnTTC Cuts at: 3684 5396 3684 Size: 1712 5555 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5075 276 Size: 53 83 186 936 3541 2468 Fragments arranged by size: 3541 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI