Restriction map for
pFN21ASDA0326
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# NO CUT ENZYMES ( 101 enzymes) #
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AatII Acc65I AccI AclI AcyI
AfaI AflIII AhdI AlwNI AocI
AscI AseI AsnI Asp718I AspEI
AspI AviII BalI BbeI BbrPI
BciVI BclI BglII BlpI Bpu1102I
BsaAI BsaHI BsiWI BsmBI Bsp1407I
BspDI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI EagI
Eam1105I EclXI Eco52I Eco81I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
Hin1I HincII HindII HpaI KasI
KpnI MfeI MluI MluNI MscI
MunI NarI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsaI
RsrII SalI SbfI ScaI SfoI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 42 TCCGGA T^CCGGA
AcsI 1869 GAATTC [GA]^AATT[CT]
AgeI 1357 ACCGGT A^CCGGT
Alw44I 705 GTGCAC G^TGCAC
Aor51HI 890 AGCGCT AGC^GCT
ApaLI 705 GTGCAC G^TGCAC
ApoI 1869 GAATTC [GA]^AATT[CT]
AvrII 2094 CCTAGG C^CTAGG
BamHI 1766 GGATCC G^GATCC
BbsI 1617 GAAGAC GAAGAC(2/6)
BbvCI 2172 CCTCAGC CCTCAGC(-5/-2)
BcgI 1055 GCAAGAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 325 GCCTGCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2094 CCTAGG C^CTAGG
BpuAI 1617 GAAGAC GAAGAC(2/6)
BsaBI 1767 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 42 TCCGGA T^CCGGA
BsiEI 2334 CGACCG CG[GA][CT]^CG
BsmI 2152 GAATGC GAATGC(1/-1)
BspEI 42 TCCGGA T^CCGGA
BspHI 1319 TCATGA T^CATGA
DraI 2140 TTTAAA TTT^AAA
DrdI 205 GACCCCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 890 AGCGCT AGC^GCT
EcoRI 1869 GAATTC G^AATTC
HgaI 1235 GCGTC GACGC(5/10)
HindIII 2163 AAGCTT A^AGCTT
KspI 1272 CCGCGG CCGC^GG
MamI 1767 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 42 TCCGGA T^CCGGA
NaeI 2545 GCCGGC GCC^GGC
NcoI 2478 CCATGG C^CATGG
NgoMIV 2545 GCCGGC G^CCGGC
PflMI 1578 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1357 ACCGGT A^CCGGT
PstI 34 CTGCAG CTGCA^G
RcaI 1319 TCATGA T^CATGA
SacII 1272 CCGCGG CCGC^GG
SfcI 34 CTGCAG C^T[GA][CT]AG
SfiI 324 GGCCTGCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1879 CCCGGG CCC^GGG
TaqI 2333 TCGA T^CGA
TfiI 160 GAATC G^A[AT]TC
TthHB8I 2333 TCGA T^CGA
Van91I 1578 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2498 CCAAAACCCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1879 CCCGGG C^CCGGG
XmaI 1879 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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AflII 1921 CTTAAG C^TTAAG
2124 CTTAAG
BfrI 1921 CTTAAG C^TTAAG
2124 CTTAAG
BsaI 140 GAGACC GGTCTC(1/5)
2449 GAGACC
BsaWI 42 TCCGGA [AT]^CCGG[AT]
1357 ACCGGT
BspMI 535 ACCTGC ACCTGC(4/8)
1103 ACCTGC
BssSI 1579 CACGAG CACGAG(-5/-1)
1714 CTCGTG
BstXI 248 CCAATCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
307 CCATCTGACTGG
EaeI 897 CGGCCA [CT]^GGCC[GA]
1065 CGGCCA
EcoNI 1104 CCTGCGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1858 CCTTTCAGAGG
MaeII 1162 ACGT A^CGT
1511 ACGT
PvuII 336 CAGCTG CAG^CTG
645 CAGCTG
SexAI 575 ACCTGGT A^CC[AT]GGT
659 ACCTGGT
StuI 323 AGGCCT AGG^CCT
1562 AGGCCT
Tsp45I 242 GTCAC ^GT[GC]AC
2416 GTCAC
Tsp509I 1870 AATT ^AATT
2088 AATT
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# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AluI AlwI ApaI
Asp700I AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SgrAI SmlI StyI Tru9I
TseI TspRI XhoII XmnI