Restriction map for
pFN21ASDA0326
(Linear) (Six-base) MAPSORT of: KIAA0326.seq Check: 1882 from: 1 to: 2607
KIAA0326.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 544 1112 2607
Size: 544 568 1495
Fragments arranged by size:
1495 568 544
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 459 627 1215 1467 1932 2607
Size: 459 168 588 252 465 675
Fragments arranged by size:
675 588 465 459 252 168
AfeI AGC'GCT
Cuts at: 0 892 2607
Size: 892 1715
AflII C'TTAA_G
Cuts at: 0 1921 2124 2607
Size: 1921 203 483
Fragments arranged by size:
1921 483 203
AgeI A'CCGG_T
Cuts at: 0 1357 2607
Size: 1357 1250
AleI CACnn'nnGTG
Cuts at: 0 71 1358 2594 2607
Size: 71 1287 1236 13
Fragments arranged by size:
1287 1236 71 13
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1768 2607
Size: 1768 839
ApaI G_GGCC'C
Cuts at: 0 28 1638 2114 2607
Size: 28 1610 476 493
Fragments arranged by size:
1610 493 476 28
ApaLI G'TGCA_C
Cuts at: 0 705 2607
Size: 705 1902
ApoI r'AATT_y
Cuts at: 0 1869 2607
Size: 1869 738
AvaI C'yCGr_G
Cuts at: 0 7 877 1465 1660 1879 2392 2533 2607
Size: 7 870 588 195 219 513 141 74
Fragments arranged by size:
870 588 513 219 195 141 74 7
AvrII C'CTAG_G
Cuts at: 0 2094 2607
Size: 2094 513
BamHI G'GATC_C
Cuts at: 0 1766 2607
Size: 1766 841
BanI G'GyrC_C
Cuts at: 0 73 500 1172 2354 2607
Size: 73 427 672 1182 253
Fragments arranged by size:
1182 672 427 253 73
BanII G_rGCy'C
Cuts at: 0 8 28 55 103 398 571 655 991
Size: 8 20 27 48 295 173 84 336
Cuts at: 991 1327 1495 1638 1661 2114 2224 2254 2284
Size: 336 168 143 23 453 110 30 30
Cuts at: 2284 2607
Size: 323
Fragments arranged by size:
453 336 336 323 295 173 168 143
110 84 48 30 30 27 23 20
8
BbsI GAAGACnn'nnnn_
Cuts at: 0 1624 2607
Size: 1624 983
BbvCI CC'TCA_GC
Cuts at: 0 2173 2607
Size: 2173 434
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1044 1078 2607
Size: 1044 34 1529
Fragments arranged by size:
1529 1044 34
BglI GCCn_nnn'nGGC
Cuts at: 0 331 2607
Size: 331 2276
Bme1580I G_kGCm'C
Cuts at: 0 28 625 709 877 1129 1213 1465 1638
Size: 28 597 84 168 252 84 252 173
Cuts at: 1638 2114 2533 2607
Size: 476 419 74
Fragments arranged by size:
597 476 419 252 252 173 168 84
84 74 28
BmgBI CAC'GTC
Cuts at: 0 1512 2607
Size: 1512 1095
BmrI ACTGGGnnnn_n'
Cuts at: 0 247 370 1123 2607
Size: 247 123 753 1484
Fragments arranged by size:
1484 753 247 123
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 213 2607
Size: 213 2394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 669 1606 2017 2287 2390 2607
Size: 208 461 937 411 270 103 217
Fragments arranged by size:
937 461 411 270 217 208 103
Bpu10I CC'TnA_GC
Cuts at: 0 1153 2173 2607
Size: 1153 1020 434
Fragments arranged by size:
1153 1020 434
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 901 2607
Size: 901 1706
BsaI GGTCTCn'nnnn_
Cuts at: 0 134 2443 2607
Size: 134 2309 164
Fragments arranged by size:
2309 164 134
BsaBI GATnn'nnATC
Cuts at: 0 1771 2607
Size: 1771 836
BsaWI w'CCGG_w
Cuts at: 0 42 1357 2607
Size: 42 1315 1250
Fragments arranged by size:
1315 1250 42
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 523 553 1975 2005 2607
Size: 523 30 1422 30 602
Fragments arranged by size:
1422 602 523 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 109 139 904 1823 2020 2607
Size: 109 30 765 919 197 587
Fragments arranged by size:
919 765 587 197 109 30
BseYI C'CCAG_C
Cuts at: 0 282 397 1001 1623 1653 2113 2241 2607
Size: 282 115 604 622 30 460 128 366
Fragments arranged by size:
622 604 460 366 282 128 115 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 601 685 942 2176 2607
Size: 436 165 84 257 1234 431
Fragments arranged by size:
1234 436 431 257 165 84
BsiEI CG_ry'CG
Cuts at: 0 2337 2607
Size: 2337 270
BsiHKAI G_wGCw'C
Cuts at: 0 571 655 709 739 991 1327 1495 1549
Size: 571 84 54 30 252 336 168 54
Cuts at: 1549 2030 2224 2254 2607
Size: 481 194 30 353
Fragments arranged by size:
571 481 353 336 252 194 168 84
54 54 30 30
BsmI GAATG_Cn'
Cuts at: 0 2158 2607
Size: 2158 449
Bsp1286I G_dGCh'C
Cuts at: 0 8 28 55 103 398 571 625 655
Size: 8 20 27 48 295 173 54 30
Cuts at: 655 709 739 877 991 1129 1213 1327 1465
Size: 54 30 138 114 138 84 114 138
Cuts at: 1465 1495 1549 1638 1661 2030 2114 2224 2254
Size: 30 54 89 23 369 84 110 30
Cuts at: 2254 2284 2533 2607
Size: 30 249 74
Fragments arranged by size:
369 295 249 173 138 138 138 114
114 110 89 84 84 74 54 54
54 48 30 30 30 30 30 27
23 20 8
BspEI T'CCGG_A
Cuts at: 0 42 2607
Size: 42 2565
BspHI T'CATG_A
Cuts at: 0 1319 2607
Size: 1319 1288
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 544 1112 2607
Size: 544 568 1495
Fragments arranged by size:
1495 568 544
BsrBI CCG'CTC
Cuts at: 0 525 1197 1281 1900 2607
Size: 525 672 84 619 707
Fragments arranged by size:
707 672 619 525 84
BsrFI r'CCGG_y
Cuts at: 0 601 769 1357 1441 2545 2607
Size: 601 168 588 84 1104 62
Fragments arranged by size:
1104 601 588 168 84 62
BssSI C'ACGA_G
Cuts at: 0 1579 1714 2607
Size: 1579 135 893
Fragments arranged by size:
1579 893 135
BstAPI GCAn_nnn'nTGC
Cuts at: 0 461 579 713 965 2607
Size: 461 118 134 252 1642
Fragments arranged by size:
1642 461 252 134 118
BstXI CCAn_nnnn'nTGG
Cuts at: 0 255 314 2607
Size: 255 59 2293
Fragments arranged by size:
2293 255 59
BstYI r'GATC_y
Cuts at: 0 30 193 266 929 1766 2607
Size: 30 163 73 663 837 841
Fragments arranged by size:
841 837 663 163 73 30
BtgI C'CryG_G
Cuts at: 0 1272 2049 2478 2607
Size: 1272 777 429 129
Fragments arranged by size:
1272 777 429 129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1794 2607
Size: 1794 813
BtsI GCAGTG_nn'
Cuts at: 0 349 1995 2607
Size: 349 1646 612
Fragments arranged by size:
1646 612 349
DraI TTT'AAA
Cuts at: 0 2142 2607
Size: 2142 465
DraIII CAC_nnn'GTG
Cuts at: 0 579 713 965 1599 2607
Size: 579 134 252 634 1008
Fragments arranged by size:
1008 634 579 252 134
DrdI GACnn_nn'nnGTC
Cuts at: 0 211 2607
Size: 211 2396
EaeI y'GGCC_r
Cuts at: 0 897 1065 2607
Size: 897 168 1542
Fragments arranged by size:
1542 897 168
EarI CTCTTCn'nnn_
Cuts at: 0 86 967 2212 2224 2242 2259 2607
Size: 86 881 1245 12 18 17 348
Fragments arranged by size:
1245 881 348 86 18 17 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 451 1327 2607
Size: 451 876 1280
Fragments arranged by size:
1280 876 451
EcoICRI GAG'CTC
Cuts at: 0 569 653 989 1325 1493 2222 2252 2607
Size: 569 84 336 336 168 729 30 355
Fragments arranged by size:
729 569 355 336 336 168 84 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 459 627 669 1215 1467 1606 1932
Size: 208 251 168 42 546 252 139 326
Cuts at: 1932 2017 2287 2390 2607
Size: 85 270 103 217
Fragments arranged by size:
546 326 270 252 251 217 208 168
139 103 85 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1108 1862 2607
Size: 1108 754 745
Fragments arranged by size:
1108 754 745
EcoO109I rG'GnC_Cy
Cuts at: 0 24 204 1113 1634 1665 1905 2110 2321
Size: 24 180 909 521 31 240 205 211
Cuts at: 2321 2397 2607
Size: 76 210
Fragments arranged by size:
909 521 240 211 210 205 180 76
31 24
EcoRI G'AATT_C
Cuts at: 0 1869 2607
Size: 1869 738
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1874 2607
Size: 1874 733
HaeII r_GCGC'y
Cuts at: 0 353 894 1160 2607
Size: 353 541 266 1447
Fragments arranged by size:
1447 541 353 266
HindIII A'AGCT_T
Cuts at: 0 2163 2607
Size: 2163 444
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 680 1268 1406 1520 1574 2607
Size: 680 588 138 114 54 1033
Fragments arranged by size:
1033 680 588 138 114 54
MslI CAynn'nnrTG
Cuts at: 0 71 1358 1522 1750 2594 2607
Size: 71 1287 164 228 844 13
Fragments arranged by size:
1287 844 228 164 71 13
MspA1I CmG'CkG
Cuts at: 0 338 647 956 1095 1274 2306 2607
Size: 338 309 309 139 179 1032 301
Fragments arranged by size:
1032 338 309 309 301 179 139
NaeI GCC'GGC
Cuts at: 0 2547 2607
Size: 2547 60
NcoI C'CATG_G
Cuts at: 0 2478 2607
Size: 2478 129
NgoMIV G'CCGG_C
Cuts at: 0 2545 2607
Size: 2545 62
PasI CC'CwG_GG
Cuts at: 0 1638 2286 2607
Size: 1638 648 321
Fragments arranged by size:
1638 648 321
PflMI CCAn_nnn'nTGG
Cuts at: 0 1584 2607
Size: 1584 1023
PfoI T'CCnGG_A
Cuts at: 0 1568 2012 2264 2607
Size: 1568 444 252 343
Fragments arranged by size:
1568 444 343 252
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1102 1134 1522 1554 2607
Size: 1102 32 388 32 1053
Fragments arranged by size:
1102 1053 388 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 204 1113 1665 2397 2607
Size: 204 909 552 732 210
Fragments arranged by size:
909 732 552 210 204
PspOMI G'GGCC_C
Cuts at: 0 24 1634 2110 2607
Size: 24 1610 476 497
Fragments arranged by size:
1610 497 476 24
PstI C_TGCA'G
Cuts at: 0 38 2607
Size: 38 2569
PvuII CAG'CTG
Cuts at: 0 338 647 2607
Size: 338 309 1960
Fragments arranged by size:
1960 338 309
SacI G_AGCT'C
Cuts at: 0 571 655 991 1327 1495 2224 2254 2607
Size: 571 84 336 336 168 729 30 353
Fragments arranged by size:
729 571 353 336 336 168 84 30
SacII CC_GC'GG
Cuts at: 0 1275 2607
Size: 1275 1332
SanDI GG'GwC_CC
Cuts at: 0 1665 2607
Size: 1665 942
SapI GCTCTTCn'nnn_
Cuts at: 0 967 2212 2242 2259 2607
Size: 967 1245 30 17 348
Fragments arranged by size:
1245 967 348 30 17
SexAI A'CCwGG_T
Cuts at: 0 575 659 2607
Size: 575 84 1948
Fragments arranged by size:
1948 575 84
SfcI C'TryA_G
Cuts at: 0 34 2607
Size: 34 2573
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 331 2607
Size: 331 2276
SgrAI Cr'CCGG_yG
Cuts at: 0 601 769 1357 1441 2607
Size: 601 168 588 84 1166
Fragments arranged by size:
1166 601 588 168 84
SmaI CCC'GGG
Cuts at: 0 1881 2607
Size: 1881 726
SmlI C'TyrA_G
Cuts at: 0 916 1921 2124 2607
Size: 916 1005 203 483
Fragments arranged by size:
1005 916 483 203
StuI AGG'CCT
Cuts at: 0 325 1564 2607
Size: 325 1239 1043
Fragments arranged by size:
1239 1043 325
StyI C'CwwG_G
Cuts at: 0 164 254 320 1849 2094 2478 2607
Size: 164 90 66 1529 245 384 129
Fragments arranged by size:
1529 384 245 164 129 90 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 745 862 2062 2386 2606 2607
Size: 745 117 1200 324 220 1
Fragments arranged by size:
1200 745 324 220 117 1
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 745 777 2607
Size: 745 32 1830
Fragments arranged by size:
1830 745 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2505 2607
Size: 2505 102
XmaI C'CCGG_G
Cuts at: 0 1879 2607
Size: 1879 728
XmnI GAAnn'nnTTC
Cuts at: 0 724 892 976 1537 1564 2607
Size: 724 168 84 561 27 1043
Fragments arranged by size:
1043 724 561 168 84 27
Enzymes that do cut:
AarI AcuI AfeI AflII AgeI AleI AlfI ApaI
ApaLI ApoI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BglI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrFI BssSI BstAPI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII HindIII MmeI
MslI MspA1I NaeI NcoI NgoMIV PasI PflMI PfoI
PpiI PpuMI PspOMI PstI PvuII SacI SacII SanDI
SapI SexAI SfcI SfiI SgrAI SmaI SmlI StuI
StyI TaqII TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflIII AhdI AloI AlwNI
AscI AseI AsiSI BaeI BbeI BciVI BclI BfrBI
BglII BlpI BmtI BsaAI BsaHI BsiWI BsmBI BsrDI
BsrGI BssHII BstBI BstEII BstZ17I Bsu36I ClaI CspCI
EagI EcoRV FseI FspI FspAI Hin4I HincII HpaI
KasI KpnI MfeI MluI MscI NarI NdeI NheI
NotI NruI NsiI NspI PacI PciI PmeI PmlI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SbfI
ScaI SfoI SgfI SnaBI SpeI SphI SrfI SspI
SwaI TatI Tth111I XbaI XhoI ZraI