(Circular) (Six-base) MAPSORT of: pFN21ASDA0323.seq Check: 1813 from: 1 to: 6886 pFN21ASDA0323 6886 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3238 3238 Size: 6886 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4696 278 Size: 53 83 186 936 3160 2468 Fragments arranged by size: 3160 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4229 1937 Size: 2292 4594 Acc65I G'GTAC_C Cuts at: 3868 4207 3868 Size: 339 6547 AclI AA'CG_TT Cuts at: 1563 5013 5386 1563 Size: 3450 373 3063 Fragments arranged by size: 3450 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2051 2233 2375 2541 3586 3762 3938 4949 Size: 419 182 142 166 1045 176 176 1011 Cuts at: 4949 6333 6601 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1045 1011 419 268 182 176 176 166 142 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6689 AflIII A'CryG_T Cuts at: 1203 5785 6762 1203 Size: 4582 977 1327 Fragments arranged by size: 4582 1327 977 AgeI A'CCGG_T Cuts at: 5717 5717 Size: 6886 AhdI GACnn_n'nnGTC Cuts at: 5615 5615 Size: 6886 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6886 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6886 AlwNI CAG_nnn'CTG Cuts at: 4072 4629 6201 4072 Size: 557 1572 4757 Fragments arranged by size: 4757 1572 557 ApaI G_GGCC'C Cuts at: 2164 2321 2164 Size: 157 6729 ApaLI G'TGCA_C Cuts at: 4943 6099 4943 Size: 1156 5730 ApoI r'AATT_y Cuts at: 1493 3911 4195 4421 4573 6682 1493 Size: 2418 284 226 152 2109 1697 Fragments arranged by size: 2418 2109 1697 284 226 152 AseI AT'TA_AT Cuts at: 160 1032 5440 160 Size: 872 4408 1606 Fragments arranged by size: 4408 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6886 AvaI C'yCGr_G Cuts at: 1870 1943 2656 3159 3357 4041 4211 1870 Size: 73 713 503 198 684 170 4545 Fragments arranged by size: 4545 713 684 503 198 170 73 BamHI G'GATC_C Cuts at: 3409 4216 4583 3409 Size: 807 367 5712 Fragments arranged by size: 5712 807 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2479 2488 3125 3214 3353 Size: 325 217 627 692 9 637 89 139 Cuts at: 3353 3868 4207 5662 618 Size: 515 339 1455 1842 Fragments arranged by size: 1842 1455 692 637 627 515 339 325 217 139 89 9 BanII G_rGCy'C Cuts at: 1391 2164 2321 2431 2786 2824 4205 1391 Size: 773 157 110 355 38 1381 4072 Fragments arranged by size: 4072 1381 773 355 157 110 38 BbeI G_GCGC'C Cuts at: 2492 3357 2492 Size: 865 6021 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3936 4043 928 Size: 971 2037 107 3771 Fragments arranged by size: 3771 2037 971 107 BbvCI CC'TCA_GC Cuts at: 3656 3656 Size: 6886 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5077 5111 1903 Size: 34 3140 34 3678 Fragments arranged by size: 3678 3140 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4778 5988 4778 Size: 1210 5676 BclI T'GATC_A Cuts at: 1548 2505 1548 Size: 957 5929 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3595 5497 136 Size: 107 122 71 631 605 1923 1902 1525 Fragments arranged by size: 1923 1902 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6881 6881 Size: 6886 BlpI GC'TnA_GC Cuts at: 2570 2785 3954 4303 2570 Size: 215 1169 349 5153 Fragments arranged by size: 5153 1169 349 215 Bme1580I G_kGCm'C Cuts at: 1790 2164 2321 2484 3130 3219 4947 6103 1790 Size: 374 157 163 646 89 1728 1156 2573 Fragments arranged by size: 2573 1728 1156 646 374 163 157 89 BmgBI CAC'GTC Cuts at: 1318 2466 3289 1318 Size: 1148 823 4915 Fragments arranged by size: 4915 1148 823 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3893 4617 5575 Size: 416 53 83 399 24 2476 724 958 Cuts at: 5575 442 Size: 1753 Fragments arranged by size: 2476 1753 958 724 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6886 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3188 3188 Size: 6886 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2333 2479 2640 3161 3488 3502 4094 5546 Size: 1094 146 161 521 327 14 592 1452 Cuts at: 5546 1239 Size: 2579 Fragments arranged by size: 2579 1452 1094 592 521 327 161 146 14 Bpu10I CC'TnA_GC Cuts at: 3656 3656 Size: 6886 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5011 5876 6174 5011 Size: 865 298 5723 Fragments arranged by size: 5723 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3252 3270 5549 882 Size: 617 1753 18 2279 2219 Fragments arranged by size: 2279 2219 1753 617 18 BsaAI yAC'GTr Cuts at: 493 1204 4183 4677 493 Size: 711 2979 494 2702 Fragments arranged by size: 2979 2702 711 494 BsaBI GATnn'nnATC Cuts at: 3815 6880 3815 Size: 3065 3821 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2489 2735 3354 4693 Size: 53 83 186 936 956 246 619 1339 Cuts at: 4693 5075 5710 275 Size: 382 635 1451 Fragments arranged by size: 1451 1339 956 936 635 619 382 246 186 83 53 BsaWI w'CCGG_w Cuts at: 2284 2600 3235 5319 5717 5991 6138 6549 2284 Size: 316 635 2084 398 274 147 411 2621 Fragments arranged by size: 2621 2084 635 411 398 316 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3500 3530 1178 Size: 30 165 30 2097 30 4534 Fragments arranged by size: 4534 2097 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2723 3089 3105 3108 3164 3177 3195 3697 Size: 1537 366 16 3 56 13 18 502 Cuts at: 3697 3809 1186 Size: 112 4263 Fragments arranged by size: 4263 1537 502 366 112 56 18 16 13 3 BseYI C'CCAG_C Cuts at: 2131 2574 2794 3218 6089 6585 2131 Size: 443 220 424 2871 496 2432 Fragments arranged by size: 2871 2432 496 443 424 220 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2393 2747 3216 3563 1163 Size: 573 657 354 469 347 4486 Fragments arranged by size: 4486 657 573 469 354 347 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4336 5097 6125 1540 Size: 275 186 2335 761 1028 2301 Fragments arranged by size: 2335 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2465 2761 2786 2803 2824 4947 5032 6103 2465 Size: 296 25 17 21 2123 85 1071 3248 Fragments arranged by size: 3248 2123 1071 296 85 25 21 17 BsmI GAATG_Cn' Cuts at: 3202 4029 4397 4490 3202 Size: 827 368 93 5598 Fragments arranged by size: 5598 827 368 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3279 3279 Size: 6886 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2164 2321 2431 2465 2484 2761 2786 Size: 399 374 157 110 34 19 277 25 Cuts at: 2786 2803 2824 3130 3219 4205 4947 5032 6103 Size: 17 21 306 89 986 742 85 1071 Cuts at: 6103 1391 Size: 2174 Fragments arranged by size: 2174 1071 986 742 399 374 306 277 157 110 89 85 34 25 21 19 17 BspEI T'CCGG_A Cuts at: 2284 2600 6549 2284 Size: 316 3949 2621 Fragments arranged by size: 3949 2621 316 BspHI T'CATG_A Cuts at: 4775 6505 4775 Size: 1730 5156 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3238 4241 844 Size: 2394 1003 3489 Fragments arranged by size: 3489 2394 1003 BsrBI CCG'CTC Cuts at: 1394 1823 2566 4054 4773 1394 Size: 429 743 1488 719 3507 Fragments arranged by size: 3507 1488 743 719 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3528 3534 5381 5555 65 Size: 1183 2280 6 1847 174 1396 Fragments arranged by size: 2280 1847 1396 1183 174 6 BsrFI r'CCGG_y Cuts at: 1700 1810 5530 5717 1700 Size: 110 3720 187 2869 Fragments arranged by size: 3720 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6886 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6886 BssSI C'ACGA_G Cuts at: 3681 4946 5958 3681 Size: 1265 1012 4609 Fragments arranged by size: 4609 1265 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6886 BstBI TT'CG_AA Cuts at: 2007 5690 2007 Size: 3683 3203 BstEII G'GTnAC_C Cuts at: 1184 3563 1184 Size: 2379 4507 BstXI CCAn_nnnn'nTGG Cuts at: 2947 2947 Size: 6886 BstYI r'GATC_y Cuts at: 1972 2858 3409 3702 4216 4583 4968 4985 6437 Size: 886 551 293 514 367 385 17 1452 Cuts at: 6437 6523 6535 6881 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 886 551 514 385 367 346 293 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3005 3005 Size: 6886 BtgI C'CryG_G Cuts at: 513 1065 3130 3245 3489 3559 513 Size: 552 2065 115 244 70 3840 Fragments arranged by size: 3840 2065 552 244 115 70 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6147 Fragments arranged by size: 6147 683 56 BtsI GCAGTG_nn' Cuts at: 2410 4402 5196 5216 2410 Size: 1992 794 20 4080 Fragments arranged by size: 4080 1992 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6886 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6851 DraI TTT'AAA Cuts at: 4190 4190 Size: 6886 DraIII CAC_nnn'GTG Cuts at: 2933 3423 4102 2933 Size: 490 679 5717 Fragments arranged by size: 5717 679 490 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3578 3940 5893 809 Size: 507 2262 362 1953 1802 Fragments arranged by size: 2262 1953 1802 507 362 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3442 3486 4333 Size: 54 1425 50 275 18 1612 44 847 Cuts at: 4333 5222 6851 8 Size: 889 1629 43 Fragments arranged by size: 1629 1612 1425 889 847 275 54 50 44 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4333 1537 Size: 275 2521 4090 Fragments arranged by size: 4090 2521 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2547 2973 3738 3866 4002 4816 1353 Size: 1194 426 765 128 136 814 3423 Fragments arranged by size: 3423 1194 814 765 426 136 128 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2794 3446 3806 5473 5847 5993 Size: 1064 138 1205 652 360 1667 374 146 Cuts at: 5993 387 Size: 1280 Fragments arranged by size: 1667 1280 1205 1064 652 374 360 146 138 EcoICRI GAG'CTC Cuts at: 2784 2822 2784 Size: 38 6848 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2233 2333 2375 2479 2541 2640 Size: 393 419 182 100 42 104 62 99 Cuts at: 2640 3161 3488 3502 3586 3762 3938 4094 4949 Size: 521 327 14 84 176 176 156 855 Cuts at: 4949 5546 6333 6601 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 855 787 597 521 419 393 327 268 182 176 176 156 104 100 99 84 62 42 14 EcoNI CCTnn'n_nnAGG Cuts at: 2273 3068 3142 3791 2273 Size: 795 74 649 5368 Fragments arranged by size: 5368 795 649 74 EcoO109I rG'GnC_Cy Cuts at: 2160 2317 2318 2359 2867 3061 3335 3400 3784 Size: 157 1 41 508 194 274 65 384 Cuts at: 3784 4159 2160 Size: 375 4887 Fragments arranged by size: 4887 508 384 375 274 194 157 65 41 1 EcoRI G'AATT_C Cuts at: 4195 4573 4195 Size: 378 6508 FspI TGC'GCA Cuts at: 5392 5392 Size: 6886 HaeII r_GCGC'y Cuts at: 1678 2492 3357 3601 6033 1678 Size: 814 865 244 2432 2531 Fragments arranged by size: 2531 2432 865 814 244 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2843 2875 5527 5559 5601 Size: 32 135 32 1271 32 2652 32 42 Cuts at: 5601 5633 1373 Size: 32 2626 Fragments arranged by size: 2652 2626 1271 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4230 4476 669 Size: 988 281 2292 246 3079 Fragments arranged by size: 3079 2292 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6886 HpaI GTT'AAC Cuts at: 4476 4476 Size: 6886 KasI G'GCGC_C Cuts at: 2488 3353 2488 Size: 865 6021 KpnI G_GTAC'C Cuts at: 3872 4211 3872 Size: 339 6547 MfeI C'AATT_G Cuts at: 4485 4485 Size: 6886 MluI A'CGCG_T Cuts at: 6762 6762 Size: 6886 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6000 6184 1305 Size: 4695 184 2007 Fragments arranged by size: 4695 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3444 3488 10 Size: 54 1425 343 1612 44 3408 Fragments arranged by size: 3408 1612 1425 343 54 44 MslI CAynn'nnrTG Cuts at: 518 1166 3539 4844 5203 5362 518 Size: 648 2373 1305 359 159 2042 Fragments arranged by size: 2373 2042 1305 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2311 3181 4069 4120 4302 4979 6127 6372 Size: 705 870 888 51 182 677 1148 245 Cuts at: 6372 1606 Size: 2120 Fragments arranged by size: 2120 1148 888 870 705 677 245 182 51 NarI GG'CG_CC Cuts at: 2489 3354 2489 Size: 865 6021 NcoI C'CATG_G Cuts at: 513 1065 3130 3245 3489 513 Size: 552 2065 115 244 3910 Fragments arranged by size: 3910 2065 552 244 115 NdeI CA'TA_TG Cuts at: 387 387 Size: 6886 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6886 NotI GC'GGCC_GC Cuts at: 4333 4333 Size: 6886 NspI r_CATG'y Cuts at: 1132 1229 4244 5789 1132 Size: 97 3015 1545 2229 Fragments arranged by size: 3015 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2431 2484 2940 3064 1348 Size: 1083 53 456 124 5170 Fragments arranged by size: 5170 1083 456 124 53 PciI A'CATG_T Cuts at: 5785 5785 Size: 6886 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6886 PfoI T'CCnGG_A Cuts at: 2703 3780 2703 Size: 1077 5809 PmeI GTTT'AAAC Cuts at: 4190 4190 Size: 6886 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4955 4987 6468 6500 4955 Size: 32 1481 32 5341 Fragments arranged by size: 5341 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2867 3784 2867 Size: 917 5969 PshAI GACnn'nnGTC Cuts at: 1613 3561 1613 Size: 1948 4938 PsiI TTA'TAA Cuts at: 4456 4456 Size: 6886 PspOMI G'GGCC_C Cuts at: 2160 2317 2160 Size: 157 6729 PstI C_TGCA'G Cuts at: 830 2058 2310 2369 2382 2652 3408 4119 4238 Size: 1228 252 59 13 270 756 711 119 Cuts at: 4238 830 Size: 3478 Fragments arranged by size: 3478 1228 756 711 270 252 119 59 13 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6886 PvuII CAG'CTG Cuts at: 2311 4069 2311 Size: 1758 5128 SacI G_AGCT'C Cuts at: 2786 2824 2786 Size: 38 6848 SalI G'TCGA_C Cuts at: 1936 4228 1936 Size: 2292 4594 SanDI GG'GwC_CC Cuts at: 2867 2867 Size: 6886 SapI GCTCTTCn'nnn_ Cuts at: 2547 2547 Size: 6886 SbfI CC_TGCA'GG Cuts at: 3408 4238 3408 Size: 830 6056 ScaI AGT'ACT Cuts at: 3548 5134 6846 3548 Size: 1586 1712 3588 Fragments arranged by size: 3588 1712 1586 SfcI C'TryA_G Cuts at: 826 1045 2054 2306 2365 2378 2648 3404 4115 Size: 219 1009 252 59 13 270 756 711 Cuts at: 4115 4234 5369 6050 6241 826 Size: 119 1135 681 191 1471 Fragments arranged by size: 1471 1135 1009 756 711 681 270 252 219 191 119 59 13 SfoI GGC'GCC Cuts at: 2490 3355 2490 Size: 865 6021 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6886 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6886 SmaI CCC'GGG Cuts at: 1872 2658 4043 4213 1872 Size: 786 1385 170 4545 Fragments arranged by size: 4545 1385 786 170 SmlI C'TyrA_G Cuts at: 820 1017 1943 4990 5891 6153 820 Size: 197 926 3047 901 262 1553 Fragments arranged by size: 3047 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4183 4677 493 Size: 3690 494 2702 Fragments arranged by size: 3690 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6886 SphI G_CATG'C Cuts at: 1132 4244 1132 Size: 3112 3774 SspI AAT'ATT Cuts at: 5 52 4810 5 Size: 47 4758 2081 Fragments arranged by size: 4758 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6886 StyI C'CwwG_G Cuts at: 513 1065 2322 2343 2809 3130 3245 3489 4325 Size: 552 1257 21 466 321 115 244 836 Cuts at: 4325 513 Size: 3074 Fragments arranged by size: 3074 1257 836 552 466 321 244 115 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2786 3011 4913 4930 5083 5268 5713 Size: 1255 1206 225 1902 17 153 185 445 Cuts at: 5713 325 Size: 1498 Fragments arranged by size: 1902 1498 1255 1206 445 225 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2615 3546 5132 Size: 274 80 33 51 1443 638 931 1586 Cuts at: 5132 6844 96 Size: 1712 138 Fragments arranged by size: 1712 1586 1443 931 638 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2917 2942 2949 2969 2974 3001 3500 3532 6846 Size: 25 7 20 5 27 499 32 3314 Cuts at: 6846 6878 2917 Size: 32 2925 Fragments arranged by size: 3314 2925 499 32 32 27 25 20 7 5 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6886 XbaI T'CTAG_A Cuts at: 4222 4222 Size: 6886 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6886 XmaI C'CCGG_G Cuts at: 1870 2656 4041 4211 1870 Size: 786 1385 170 4545 Fragments arranged by size: 4545 1385 786 170 XmnI GAAnn'nnTTC Cuts at: 5015 5015 Size: 6886 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4694 276 Size: 53 83 186 936 3160 2468 Fragments arranged by size: 3160 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI XcmI