(Circular) (Six-base) MAPSORT of: pFN21ASDA0319.seq Check: 7161 from: 1 to: 7945 pFN21ASDA0319 7945 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3374 5755 278 Size: 53 83 186 936 1838 2381 2468 Fragments arranged by size: 2468 2381 1838 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2187 3848 4250 5288 1937 Size: 250 1661 402 1038 4594 Fragments arranged by size: 4594 1661 1038 402 250 Acc65I G'GTAC_C Cuts at: 5266 5266 Size: 7945 AclI AA'CG_TT Cuts at: 1563 6072 6445 1563 Size: 4509 373 3063 Fragments arranged by size: 4509 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2736 3104 3453 3839 4692 4787 5128 5200 Size: 1104 368 349 386 853 95 341 72 Cuts at: 5200 6008 7392 7660 1632 Size: 808 1384 268 1917 Fragments arranged by size: 1917 1384 1104 853 808 386 368 349 341 268 95 72 AfeI AGC'GCT Cuts at: 4808 4808 Size: 7945 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7748 AflIII A'CryG_T Cuts at: 1203 3742 4201 6844 7821 1203 Size: 2539 459 2643 977 1327 Fragments arranged by size: 2643 2539 1327 977 459 AgeI A'CCGG_T Cuts at: 6776 6776 Size: 7945 AhdI GACnn_n'nnGTC Cuts at: 3230 6674 3230 Size: 3444 4501 AleI CACnn'nnGTG Cuts at: 1166 2039 2327 4532 1166 Size: 873 288 2205 4579 Fragments arranged by size: 4579 2205 873 288 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7945 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2378 2410 2808 2840 2378 Size: 32 398 32 7483 Fragments arranged by size: 7483 398 32 32 AlwNI CAG_nnn'CTG Cuts at: 2362 3902 4123 4310 4430 5688 7260 2362 Size: 1540 221 187 120 1258 1572 3047 Fragments arranged by size: 3047 1572 1540 1258 221 187 120 ApaI G_GGCC'C Cuts at: 2306 3450 4600 2306 Size: 1144 1150 5651 Fragments arranged by size: 5651 1150 1144 ApaLI G'TGCA_C Cuts at: 6002 7158 6002 Size: 1156 6789 ApoI r'AATT_y Cuts at: 1493 3139 5254 5480 5632 7741 1493 Size: 1646 2115 226 152 2109 1697 Fragments arranged by size: 2115 2109 1697 1646 226 152 AseI AT'TA_AT Cuts at: 160 1032 6499 160 Size: 872 5467 1606 Fragments arranged by size: 5467 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7945 AvaI C'yCGr_G Cuts at: 1870 1943 2401 2911 4185 5270 1870 Size: 73 458 510 1274 1085 4545 Fragments arranged by size: 4545 1274 1085 510 458 73 AvrII C'CTAG_G Cuts at: 4457 4457 Size: 7945 BamHI G'GATC_C Cuts at: 4284 5275 5642 4284 Size: 991 367 6587 Fragments arranged by size: 6587 991 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2026 2635 5266 6721 618 Size: 325 217 627 239 609 2631 1455 1842 Fragments arranged by size: 2631 1842 1455 627 609 325 239 217 BanII G_rGCy'C Cuts at: 1391 2292 2306 2396 2550 2667 2868 3357 3450 Size: 901 14 90 154 117 201 489 93 Cuts at: 3450 4186 4600 5264 1391 Size: 736 414 664 4072 Fragments arranged by size: 4072 901 736 664 489 414 201 154 117 93 90 14 BbeI G_GCGC'C Cuts at: 2030 2030 Size: 7945 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2837 3226 3470 3982 928 Size: 971 938 389 244 512 4891 Fragments arranged by size: 4891 971 938 512 389 244 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6136 6170 1903 Size: 34 4199 34 3678 Fragments arranged by size: 4199 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5116 5837 7047 5116 Size: 721 1210 6014 Fragments arranged by size: 6014 1210 721 BclI T'GATC_A Cuts at: 1548 1548 Size: 7945 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6556 136 Size: 107 122 71 631 605 4884 1525 Fragments arranged by size: 4884 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7940 7940 Size: 7945 BlpI GC'TnA_GC Cuts at: 2867 5362 2867 Size: 2495 5450 Bme1580I G_kGCm'C Cuts at: 1790 2086 2306 3450 4600 6006 7162 1790 Size: 296 220 1144 1150 1406 1156 2573 Fragments arranged by size: 2573 1406 1156 1150 1144 296 220 BmgBI CAC'GTC Cuts at: 1318 4009 4134 1318 Size: 2691 125 5129 Fragments arranged by size: 5129 2691 125 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2526 2584 2888 Size: 416 53 83 399 24 1109 58 304 Cuts at: 2888 3929 4009 4014 5676 6634 442 Size: 1041 80 5 1662 958 1753 Fragments arranged by size: 1753 1662 1109 1041 958 416 399 304 83 80 58 53 24 5 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7945 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2887 5107 2887 Size: 2220 5725 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2531 2774 2882 3060 3879 4058 4332 5077 Size: 1292 243 108 178 819 179 274 745 Cuts at: 5077 6605 1239 Size: 1528 2579 Fragments arranged by size: 2579 1528 1292 819 745 274 243 179 178 108 Bpu10I CC'TnA_GC Cuts at: 2661 2661 Size: 7945 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4759 6070 6935 7233 4759 Size: 1311 865 298 5471 Fragments arranged by size: 5471 1311 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4076 6608 882 Size: 617 2577 2532 2219 Fragments arranged by size: 2577 2532 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5242 5736 493 Size: 711 4038 494 2702 Fragments arranged by size: 4038 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7939 7939 Size: 7945 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2027 3371 3767 5752 Size: 53 83 186 936 494 1344 396 1985 Cuts at: 5752 6134 6769 275 Size: 382 635 1451 Fragments arranged by size: 1985 1451 1344 936 635 494 396 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6378 6776 7050 7197 7608 6378 Size: 398 274 147 411 6715 Fragments arranged by size: 6715 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2378 2408 2989 3019 4361 Size: 30 165 30 975 30 581 30 1342 Cuts at: 4361 4391 1178 Size: 30 4732 Fragments arranged by size: 4732 1342 975 581 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2489 2602 1186 Size: 1303 113 6529 Fragments arranged by size: 6529 1303 113 BseYI C'CCAG_C Cuts at: 7148 7644 7148 Size: 496 7449 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2116 2176 2337 4044 4466 4673 1163 Size: 573 380 60 161 1707 422 207 4435 Fragments arranged by size: 4435 1707 573 422 380 207 161 60 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2199 5395 6156 7184 1540 Size: 275 186 198 3196 761 1028 2301 Fragments arranged by size: 3196 2301 1028 761 275 198 186 BsiHKAI G_wGCw'C Cuts at: 2046 2334 2667 2868 2901 2928 3357 4008 5088 Size: 288 333 201 33 27 429 651 1080 Cuts at: 5088 6006 6091 7162 2046 Size: 918 85 1071 2829 Fragments arranged by size: 2829 1080 1071 918 651 429 333 288 201 85 33 27 BsmI GAATG_Cn' Cuts at: 5456 5549 5456 Size: 93 7852 BsmBI CGTCTCn'nnnn_ Cuts at: 2639 2639 Size: 7945 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2086 2292 2306 2334 2396 2550 Size: 399 256 40 206 14 28 62 154 Cuts at: 2550 2667 2868 2901 2928 3357 3450 4008 4186 Size: 117 201 33 27 429 93 558 178 Cuts at: 4186 4600 5088 5264 6006 6091 7162 1391 Size: 414 488 176 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 558 488 429 414 399 256 206 201 178 176 154 117 93 85 62 40 33 28 27 14 BspEI T'CCGG_A Cuts at: 7608 7608 Size: 7945 BspHI T'CATG_A Cuts at: 5834 7564 5834 Size: 1730 6215 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2066 3324 4480 4745 5300 844 Size: 1222 1258 1156 265 555 3489 Fragments arranged by size: 3489 1258 1222 1156 555 265 BsrBI CCG'CTC Cuts at: 1394 1823 5832 1394 Size: 429 4009 3507 Fragments arranged by size: 4009 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2051 3795 6440 6614 65 Size: 1183 803 1744 2645 174 1396 Fragments arranged by size: 2645 1744 1396 1183 803 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3908 6589 6776 1700 Size: 110 2098 2681 187 2869 Fragments arranged by size: 2869 2681 2098 187 110 BsrGI T'GTAC_A Cuts at: 96 4377 4633 96 Size: 4281 256 3408 Fragments arranged by size: 4281 3408 256 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7945 BssSI C'ACGA_G Cuts at: 6005 7017 6005 Size: 1012 6933 BstAPI GCAn_nnn'nTGC Cuts at: 794 2081 4959 794 Size: 1287 2878 3780 Fragments arranged by size: 3780 2878 1287 BstBI TT'CG_AA Cuts at: 2007 6749 2007 Size: 4742 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7945 BstXI CCAn_nnnn'nTGG Cuts at: 2299 4027 4234 5101 2299 Size: 1728 207 867 5143 Fragments arranged by size: 5143 1728 867 207 BstYI r'GATC_y Cuts at: 1972 2406 4284 4606 5275 5642 6027 6044 7496 Size: 434 1878 322 669 367 385 17 1452 Cuts at: 7496 7582 7594 7940 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1878 1452 669 434 385 367 346 322 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2424 2424 Size: 7945 BtgI C'CryG_G Cuts at: 513 1065 2022 2880 3979 4780 513 Size: 552 957 858 1099 801 3678 Fragments arranged by size: 3678 1099 957 858 801 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3987 487 Size: 683 56 2761 4445 Fragments arranged by size: 4445 2761 683 56 BtsI GCAGTG_nn' Cuts at: 2099 3000 3688 4032 4428 4786 5461 6255 6275 Size: 901 688 344 396 358 675 794 20 Cuts at: 6275 2099 Size: 3769 Fragments arranged by size: 3769 901 794 688 675 396 358 344 20 ClaI AT'CG_AT Cuts at: 1554 4323 1554 Size: 2769 5176 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7910 DraI TTT'AAA Cuts at: 5249 5249 Size: 7945 DraIII CAC_nnn'GTG Cuts at: 4342 4342 Size: 7945 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3482 4580 6952 809 Size: 507 2166 1098 2372 1802 Fragments arranged by size: 2372 2166 1802 1098 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2196 3906 4294 Size: 54 1425 50 275 18 366 1710 388 Cuts at: 4294 4777 5392 6281 7910 8 Size: 483 615 889 1629 43 Fragments arranged by size: 1710 1629 1425 889 615 483 388 366 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2196 5392 1537 Size: 275 384 3196 4090 Fragments arranged by size: 4090 3196 384 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2053 3301 3453 4457 5875 1353 Size: 700 1248 152 1004 1418 3423 Fragments arranged by size: 3423 1418 1248 1004 700 152 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6532 6906 7052 387 Size: 1064 138 4943 374 146 1280 Fragments arranged by size: 4943 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2665 2866 3355 2665 Size: 201 489 7255 Fragments arranged by size: 7255 489 201 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2531 2736 2774 2882 3060 3104 3453 Size: 393 899 205 38 108 178 44 349 Cuts at: 3453 3839 3879 4058 4332 4692 4787 5077 5128 Size: 386 40 179 274 360 95 290 51 Cuts at: 5128 5200 6008 6605 7392 7660 1239 Size: 72 808 597 787 268 1524 Fragments arranged by size: 1524 899 808 787 597 393 386 360 349 290 274 268 205 179 178 108 95 72 51 44 40 38 EcoO109I rG'GnC_Cy Cuts at: 2303 2467 2518 2578 2601 2656 3447 3722 4016 Size: 164 51 60 23 55 791 275 294 Cuts at: 4016 4083 4413 2303 Size: 67 330 5835 Fragments arranged by size: 5835 791 330 294 275 164 67 60 55 51 23 EcoRI G'AATT_C Cuts at: 5254 5632 5254 Size: 378 7567 EcoRV GAT'ATC Cuts at: 2431 2431 Size: 7945 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2539 4735 2539 Size: 2196 5749 FseI GG_CCGG'CC Cuts at: 3912 3912 Size: 7945 FspI TGC'GCA Cuts at: 6451 6451 Size: 7945 HaeII r_GCGC'y Cuts at: 1678 2030 3107 4810 7092 1678 Size: 352 1077 1703 2282 2531 Fragments arranged by size: 2531 2282 1703 1077 352 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2465 2497 3216 3248 4305 Size: 32 135 32 893 32 719 32 1057 Cuts at: 4305 4337 5155 5187 6586 6618 6660 6692 1373 Size: 32 818 32 1399 32 42 32 2626 Fragments arranged by size: 2626 1399 1057 893 818 719 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3313 3475 3531 5289 5535 669 Size: 988 281 1375 162 56 1758 246 3079 Fragments arranged by size: 3079 1758 1375 988 281 246 162 56 HindIII A'AGCT_T Cuts at: 748 748 Size: 7945 HpaI GTT'AAC Cuts at: 5535 5535 Size: 7945 KasI G'GCGC_C Cuts at: 2026 2026 Size: 7945 KpnI G_GTAC'C Cuts at: 5270 5270 Size: 7945 MfeI C'AATT_G Cuts at: 2070 3212 5544 2070 Size: 1142 2332 4471 Fragments arranged by size: 4471 2332 1142 MluI A'CGCG_T Cuts at: 7821 7821 Size: 7945 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2599 2745 3202 7059 7243 1305 Size: 1294 146 457 3857 184 2007 Fragments arranged by size: 3857 2007 1294 457 184 146 MscI TGG'CCA Cuts at: 10 64 1489 1832 4296 4779 10 Size: 54 1425 343 2464 483 3176 Fragments arranged by size: 3176 2464 1425 483 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2039 2162 2327 4532 5903 6262 6421 Size: 648 873 123 165 2205 1371 359 159 Cuts at: 6421 518 Size: 2042 Fragments arranged by size: 2205 2042 1371 873 648 359 165 159 123 MspA1I CmG'CkG Cuts at: 1606 2220 2250 2359 2642 3817 4310 4507 4519 Size: 614 30 109 283 1175 493 197 12 Cuts at: 4519 4543 5361 6038 7186 7431 1606 Size: 24 818 677 1148 245 2120 Fragments arranged by size: 2120 1175 1148 818 677 614 493 283 245 197 109 30 24 12 NaeI GCC'GGC Cuts at: 3910 3910 Size: 7945 NarI GG'CG_CC Cuts at: 2027 2027 Size: 7945 NcoI C'CATG_G Cuts at: 513 1065 2022 4780 513 Size: 552 957 2758 3678 Fragments arranged by size: 3678 2758 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7945 NgoMIV G'CCGG_C Cuts at: 3908 3908 Size: 7945 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7945 NotI GC'GGCC_GC Cuts at: 5392 5392 Size: 7945 NspI r_CATG'y Cuts at: 1132 1229 3746 4205 4696 5303 6848 1132 Size: 97 2517 459 491 607 1545 2229 Fragments arranged by size: 2517 2229 1545 607 491 459 97 PasI CC'CwG_GG Cuts at: 1348 3718 1348 Size: 2370 5575 PciI A'CATG_T Cuts at: 3742 4201 6844 3742 Size: 459 2643 4843 Fragments arranged by size: 4843 2643 459 PflMI CCAn_nnn'nTGG Cuts at: 1108 4310 4342 1108 Size: 3202 32 4711 Fragments arranged by size: 4711 3202 32 PfoI T'CCnGG_A Cuts at: 2790 4287 5013 2790 Size: 1497 726 5722 Fragments arranged by size: 5722 1497 726 PmeI GTTT'AAAC Cuts at: 5249 5249 Size: 7945 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2378 2410 6014 6046 7527 7559 2378 Size: 32 3604 32 1481 32 2764 Fragments arranged by size: 3604 2764 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2518 2656 3722 4083 2518 Size: 138 1066 361 6380 Fragments arranged by size: 6380 1066 361 138 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7945 PsiI TTA'TAA Cuts at: 3056 5515 3056 Size: 2459 5486 PspOMI G'GGCC_C Cuts at: 2302 3446 4596 2302 Size: 1144 1150 5651 Fragments arranged by size: 5651 1150 1144 PstI C_TGCA'G Cuts at: 830 3574 4353 4491 5297 830 Size: 2744 779 138 806 3478 Fragments arranged by size: 3478 2744 806 779 138 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7945 PvuII CAG'CTG Cuts at: 2220 2359 3817 4310 4507 4543 2220 Size: 139 1458 493 197 36 5622 Fragments arranged by size: 5622 1458 493 197 139 36 SacI G_AGCT'C Cuts at: 2667 2868 3357 2667 Size: 201 489 7255 Fragments arranged by size: 7255 489 201 SalI G'TCGA_C Cuts at: 1936 5287 1936 Size: 3351 4594 SbfI CC_TGCA'GG Cuts at: 4491 5297 4491 Size: 806 7139 ScaI AGT'ACT Cuts at: 6193 7905 6193 Size: 1712 6233 SexAI A'CCwGG_T Cuts at: 2256 2256 Size: 7945 SfcI C'TryA_G Cuts at: 826 1045 3111 3324 3517 3570 3855 4349 4487 Size: 219 2066 213 193 53 285 494 138 Cuts at: 4487 5293 6428 7109 7300 826 Size: 806 1135 681 191 1471 Fragments arranged by size: 2066 1471 1135 806 681 494 285 219 213 193 191 138 53 SfoI GGC'GCC Cuts at: 2028 2028 Size: 7945 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7945 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7945 SmaI CCC'GGG Cuts at: 1872 2913 4187 5272 1872 Size: 1041 1274 1085 4545 Fragments arranged by size: 4545 1274 1085 1041 SmlI C'TyrA_G Cuts at: 820 1017 1943 4738 6049 6950 7212 820 Size: 197 926 2795 1311 901 262 1553 Fragments arranged by size: 2795 1553 1311 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5242 5736 493 Size: 4749 494 2702 Fragments arranged by size: 4749 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7945 SphI G_CATG'C Cuts at: 1132 5303 1132 Size: 4171 3774 SrfI GCCC'GGGC Cuts at: 4187 4187 Size: 7945 SspI AAT'ATT Cuts at: 5 52 4042 5869 5 Size: 47 3990 1827 2081 Fragments arranged by size: 3990 2081 1827 47 StuI AGG'CCT Cuts at: 1517 2350 3089 1517 Size: 833 739 6373 Fragments arranged by size: 6373 833 739 StyI C'CwwG_G Cuts at: 513 1065 2022 3166 4457 4780 5384 513 Size: 552 957 1144 1291 323 604 3074 Fragments arranged by size: 3074 1291 1144 957 604 552 323 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5972 5989 6142 6327 6772 325 Size: 1255 4392 17 153 185 445 1498 Fragments arranged by size: 4392 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2565 2918 3398 Size: 274 80 33 51 1443 588 353 480 Cuts at: 3398 3760 4377 4633 5004 6191 7903 96 Size: 362 617 256 371 1187 1712 138 Fragments arranged by size: 1712 1443 1187 617 588 480 371 362 353 274 256 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7905 7937 7905 Size: 32 7913 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7945 XbaI T'CTAG_A Cuts at: 5281 5281 Size: 7945 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2224 4144 2224 Size: 1920 6025 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7945 XmaI C'CCGG_G Cuts at: 1870 2911 4185 5270 1870 Size: 1041 1274 1085 4545 Fragments arranged by size: 4545 1274 1085 1041 XmnI GAAnn'nnTTC Cuts at: 6074 6074 Size: 7945 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3372 5753 276 Size: 53 83 186 936 1838 2381 2468 Fragments arranged by size: 2468 2381 1838 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI BaeI BbvCI BfrBI BsiWI BstZ17I EcoNI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SwaI