Restriction map for
pFN21ASDA0319
(Linear) (Six-base) MAPSORT of: KIAA0319.seq Check: 9942 from: 1 to: 3216
KIAA0319.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AatII G_ACGT'C
Cuts at: 0 1351 3216
Size: 1351 1865
AccI GT'mk_AC
Cuts at: 0 164 1825 2227 3216
Size: 164 1661 402 989
Fragments arranged by size:
1661 989 402 164
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 713 1081 1430 1816 2669 2764 3105 3177
Size: 713 368 349 386 853 95 341 72
Cuts at: 3177 3216
Size: 39
Fragments arranged by size:
853 713 386 368 349 341 95 72
39
AfeI AGC'GCT
Cuts at: 0 2785 3216
Size: 2785 431
AflIII A'CryG_T
Cuts at: 0 1719 2178 3216
Size: 1719 459 1038
Fragments arranged by size:
1719 1038 459
AhdI GACnn_n'nnGTC
Cuts at: 0 1207 3216
Size: 1207 2009
AleI CACnn'nnGTG
Cuts at: 0 16 304 2509 3216
Size: 16 288 2205 707
Fragments arranged by size:
2205 707 288 16
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 355 387 785 817 3216
Size: 355 32 398 32 2399
Fragments arranged by size:
2399 398 355 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 339 1879 2100 2287 2407 3216
Size: 339 1540 221 187 120 809
Fragments arranged by size:
1540 809 339 221 187 120
ApaI G_GGCC'C
Cuts at: 0 283 1427 2577 3216
Size: 283 1144 1150 639
Fragments arranged by size:
1150 1144 639 283
ApoI r'AATT_y
Cuts at: 0 1116 3216
Size: 1116 2100
AvaI C'yCGr_G
Cuts at: 0 378 888 2162 3216
Size: 378 510 1274 1054
Fragments arranged by size:
1274 1054 510 378
AvrII C'CTAG_G
Cuts at: 0 2434 3216
Size: 2434 782
BamHI G'GATC_C
Cuts at: 0 2261 3216
Size: 2261 955
BanI G'GyrC_C
Cuts at: 0 3 612 3216
Size: 3 609 2604
Fragments arranged by size:
2604 609 3
BanII G_rGCy'C
Cuts at: 0 269 283 373 527 644 845 1334 1427
Size: 269 14 90 154 117 201 489 93
Cuts at: 1427 2163 2577 3216
Size: 736 414 639
Fragments arranged by size:
736 639 489 414 269 201 154 117
93 90 14
BbeI G_GCGC'C
Cuts at: 0 7 3216
Size: 7 3209
BbsI GAAGACnn'nnnn_
Cuts at: 0 814 1203 1447 1959 3216
Size: 814 389 244 512 1257
Fragments arranged by size:
1257 814 512 389 244
BciVI GTATCCnnnnn_n'
Cuts at: 0 3093 3216
Size: 3093 123
BlpI GC'TnA_GC
Cuts at: 0 844 3216
Size: 844 2372
Bme1580I G_kGCm'C
Cuts at: 0 63 283 1427 2577 3216
Size: 63 220 1144 1150 639
Fragments arranged by size:
1150 1144 639 220 63
BmgBI CAC'GTC
Cuts at: 0 1986 2111 3216
Size: 1986 125 1105
Fragments arranged by size:
1986 1105 125
BmrI ACTGGGnnnn_n'
Cuts at: 0 503 561 865 1906 1986 1991 3216
Size: 503 58 304 1041 80 5 1225
Fragments arranged by size:
1225 1041 503 304 80 58 5
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 864 3084 3216
Size: 864 2220 132
Fragments arranged by size:
2220 864 132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 751 859 1037 1856 2035 2309 3054
Size: 508 243 108 178 819 179 274 745
Cuts at: 3054 3216
Size: 162
Fragments arranged by size:
819 745 508 274 243 179 178 162
108
Bpu10I CC'TnA_GC
Cuts at: 0 638 3216
Size: 638 2578
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2736 3216
Size: 2736 480
BsaI GGTCTCn'nnnn_
Cuts at: 0 2053 3216
Size: 2053 1163
BsaHI Gr'CG_yC
Cuts at: 0 4 1348 1744 3216
Size: 4 1344 396 1472
Fragments arranged by size:
1472 1344 396 4
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 355 385 966 996 2338 2368 3216
Size: 355 30 581 30 1342 30 848
Fragments arranged by size:
1342 848 581 355 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 466 579 3216
Size: 466 113 2637
Fragments arranged by size:
2637 466 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 153 314 2021 2443 2650 3216
Size: 93 60 161 1707 422 207 566
Fragments arranged by size:
1707 566 422 207 161 93 60
BsiEI CG_ry'CG
Cuts at: 0 176 3216
Size: 176 3040
BsiHKAI G_wGCw'C
Cuts at: 0 23 311 644 845 878 905 1334 1985
Size: 23 288 333 201 33 27 429 651
Cuts at: 1985 3065 3216
Size: 1080 151
Fragments arranged by size:
1080 651 429 333 288 201 151 33
27 23
BsmBI CGTCTCn'nnnn_
Cuts at: 0 616 3216
Size: 616 2600
Bsp1286I G_dGCh'C
Cuts at: 0 23 63 269 283 311 373 527 644
Size: 23 40 206 14 28 62 154 117
Cuts at: 644 845 878 905 1334 1427 1985 2163 2577
Size: 201 33 27 429 93 558 178 414
Cuts at: 2577 3065 3216
Size: 488 151
Fragments arranged by size:
558 488 429 414 206 201 178 154
151 117 93 62 40 33 28 27
23 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 43 1301 2457 2722 3216
Size: 43 1258 1156 265 494
Fragments arranged by size:
1258 1156 494 265 43
BsrDI GCAATG_nn'
Cuts at: 0 28 1772 3216
Size: 28 1744 1444
Fragments arranged by size:
1744 1444 28
BsrFI r'CCGG_y
Cuts at: 0 1885 3216
Size: 1885 1331
BsrGI T'GTAC_A
Cuts at: 0 2354 2610 3216
Size: 2354 256 606
Fragments arranged by size:
2354 606 256
BstAPI GCAn_nnn'nTGC
Cuts at: 0 58 2936 3216
Size: 58 2878 280
Fragments arranged by size:
2878 280 58
BstXI CCAn_nnnn'nTGG
Cuts at: 0 276 2004 2211 3078 3216
Size: 276 1728 207 867 138
Fragments arranged by size:
1728 867 276 207 138
BstYI r'GATC_y
Cuts at: 0 383 2261 2583 3216
Size: 383 1878 322 633
Fragments arranged by size:
1878 633 383 322
Bsu36I CC'TnA_GG
Cuts at: 0 401 3216
Size: 401 2815
BtgI C'CryG_G
Cuts at: 0 857 1956 2757 3216
Size: 857 1099 801 459
Fragments arranged by size:
1099 857 801 459
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1964 3216
Size: 1964 1252
BtsI GCAGTG_nn'
Cuts at: 0 76 977 1665 2009 2405 2763 3216
Size: 76 901 688 344 396 358 453
Fragments arranged by size:
901 688 453 396 358 344 76
ClaI AT'CG_AT
Cuts at: 0 2300 3216
Size: 2300 916
DraIII CAC_nnn'GTG
Cuts at: 0 2319 3216
Size: 2319 897
DrdI GACnn_nn'nnGTC
Cuts at: 0 1459 2557 3216
Size: 1459 1098 659
Fragments arranged by size:
1459 1098 659
EaeI y'GGCC_r
Cuts at: 0 173 1883 2271 2754 3216
Size: 173 1710 388 483 462
Fragments arranged by size:
1710 483 462 388 173
EagI C'GGCC_G
Cuts at: 0 173 3216
Size: 173 3043
EarI CTCTTCn'nnn_
Cuts at: 0 30 1278 1430 2434 3216
Size: 30 1248 152 1004 782
Fragments arranged by size:
1248 1004 782 152 30
EcoICRI GAG'CTC
Cuts at: 0 642 843 1332 3216
Size: 642 201 489 1884
Fragments arranged by size:
1884 642 489 201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 713 751 859 1037 1081 1430 1816
Size: 508 205 38 108 178 44 349 386
Cuts at: 1816 1856 2035 2309 2669 2764 3054 3105 3177
Size: 40 179 274 360 95 290 51 72
Cuts at: 3177 3216
Size: 39
Fragments arranged by size:
508 386 360 349 290 274 205 179
178 108 95 72 51 44 40 39
38
EcoO109I rG'GnC_Cy
Cuts at: 0 280 444 495 555 578 633 1424 1699
Size: 280 164 51 60 23 55 791 275
Cuts at: 1699 1993 2060 2390 3216
Size: 294 67 330 826
Fragments arranged by size:
826 791 330 294 280 275 164 67
60 55 51 23
EcoRV GAT'ATC
Cuts at: 0 408 3216
Size: 408 2808
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 516 2712 3216
Size: 516 2196 504
Fragments arranged by size:
2196 516 504
FseI GG_CCGG'CC
Cuts at: 0 1889 3216
Size: 1889 1327
HaeII r_GCGC'y
Cuts at: 0 7 1084 2787 3216
Size: 7 1077 1703 429
Fragments arranged by size:
1703 1077 429 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 442 474 1193 1225 2282 2314 3132 3164
Size: 442 32 719 32 1057 32 818 32
Cuts at: 3164 3216
Size: 52
Fragments arranged by size:
1057 818 719 442 52 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1290 1452 1508 3216
Size: 1290 162 56 1708
Fragments arranged by size:
1708 1290 162 56
KasI G'GCGC_C
Cuts at: 0 3 3216
Size: 3 3213
MfeI C'AATT_G
Cuts at: 0 47 1189 3216
Size: 47 1142 2027
Fragments arranged by size:
2027 1142 47
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 576 722 1179 3216
Size: 576 146 457 2037
Fragments arranged by size:
2037 576 457 146
MscI TGG'CCA
Cuts at: 0 2273 2756 3216
Size: 2273 483 460
Fragments arranged by size:
2273 483 460
MslI CAynn'nnrTG
Cuts at: 0 16 139 304 2509 3216
Size: 16 123 165 2205 707
Fragments arranged by size:
2205 707 165 123 16
MspA1I CmG'CkG
Cuts at: 0 197 227 336 619 1794 2287 2484 2496
Size: 197 30 109 283 1175 493 197 12
Cuts at: 2496 2520 3216
Size: 24 696
Fragments arranged by size:
1175 696 493 283 197 197 109 30
24 12
NaeI GCC'GGC
Cuts at: 0 1887 3216
Size: 1887 1329
NarI GG'CG_CC
Cuts at: 0 4 3216
Size: 4 3212
NcoI C'CATG_G
Cuts at: 0 2757 3216
Size: 2757 459
NgoMIV G'CCGG_C
Cuts at: 0 1885 3216
Size: 1885 1331
NspI r_CATG'y
Cuts at: 0 1723 2182 2673 3216
Size: 1723 459 491 543
Fragments arranged by size:
1723 543 491 459
PasI CC'CwG_GG
Cuts at: 0 1695 3216
Size: 1695 1521
PciI A'CATG_T
Cuts at: 0 1719 2178 3216
Size: 1719 459 1038
Fragments arranged by size:
1719 1038 459
PflMI CCAn_nnn'nTGG
Cuts at: 0 2287 2319 3216
Size: 2287 32 897
Fragments arranged by size:
2287 897 32
PfoI T'CCnGG_A
Cuts at: 0 767 2264 2990 3216
Size: 767 1497 726 226
Fragments arranged by size:
1497 767 726 226
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 355 387 3216
Size: 355 32 2829
Fragments arranged by size:
2829 355 32
PpuMI rG'GwC_Cy
Cuts at: 0 495 633 1699 2060 3216
Size: 495 138 1066 361 1156
Fragments arranged by size:
1156 1066 495 361 138
PsiI TTA'TAA
Cuts at: 0 1033 3216
Size: 1033 2183
PspOMI G'GGCC_C
Cuts at: 0 279 1423 2573 3216
Size: 279 1144 1150 643
Fragments arranged by size:
1150 1144 643 279
PstI C_TGCA'G
Cuts at: 0 1551 2330 2468 3216
Size: 1551 779 138 748
Fragments arranged by size:
1551 779 748 138
PvuII CAG'CTG
Cuts at: 0 197 336 1794 2287 2484 2520 3216
Size: 197 139 1458 493 197 36 696
Fragments arranged by size:
1458 696 493 197 197 139 36
SacI G_AGCT'C
Cuts at: 0 644 845 1334 3216
Size: 644 201 489 1882
Fragments arranged by size:
1882 644 489 201
SbfI CC_TGCA'GG
Cuts at: 0 2468 3216
Size: 2468 748
SexAI A'CCwGG_T
Cuts at: 0 233 3216
Size: 233 2983
SfcI C'TryA_G
Cuts at: 0 1088 1301 1494 1547 1832 2326 2464 3216
Size: 1088 213 193 53 285 494 138 752
Fragments arranged by size:
1088 752 494 285 213 193 138 53
SfoI GGC'GCC
Cuts at: 0 5 3216
Size: 5 3211
SmaI CCC'GGG
Cuts at: 0 890 2164 3216
Size: 890 1274 1052
Fragments arranged by size:
1274 1052 890
SmlI C'TyrA_G
Cuts at: 0 2715 3216
Size: 2715 501
SrfI GCCC'GGGC
Cuts at: 0 2164 3216
Size: 2164 1052
SspI AAT'ATT
Cuts at: 0 2019 3216
Size: 2019 1197
StuI AGG'CCT
Cuts at: 0 327 1066 3216
Size: 327 739 2150
Fragments arranged by size:
2150 739 327
StyI C'CwwG_G
Cuts at: 0 1143 2434 2757 3216
Size: 1143 1291 323 459
Fragments arranged by size:
1291 1143 459 323
TatI w'GTAC_w
Cuts at: 0 542 895 1375 1737 2354 2610 2981 3216
Size: 542 353 480 362 617 256 371 235
Fragments arranged by size:
617 542 480 371 362 353 256 235
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 201 2121 3216
Size: 201 1920 1095
Fragments arranged by size:
1920 1095 201
XmaI C'CCGG_G
Cuts at: 0 888 2162 3216
Size: 888 1274 1054
Fragments arranged by size:
1274 1054 888
ZraI GAC'GTC
Cuts at: 0 1349 3216
Size: 1349 1867
Enzymes that do cut:
AatII AccI AcuI AfeI AflIII AhdI AleI AloI
AlwNI ApaI ApoI AvaI AvrII BamHI BanI BanII
BbeI BbsI BciVI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BsrGI
BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI EcoO109I
EcoRV FalI FseI HaeII Hin4I HincII KasI MfeI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PpiI PpuMI PsiI
PspOMI PstI PvuII SacI SbfI SexAI SfcI SfoI
SmaI SmlI SrfI SspI StuI StyI TatI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AgeI AlfI ApaLI AscI
AseI AsiSI BaeI BbvCI BcgI BclI BfrBI BglI
BglII BmtI BsaAI BsaBI BsaWI BseYI BsiWI BsmI
BspEI BspHI BsrBI BssHII BssSI BstBI BstEII BstZ17I
CspCI DraI EciI EcoNI EcoRI FspI FspAI HindIII
HpaI KpnI MluI NdeI NheI NotI NruI NsiI
PacI PmeI PmlI PshAI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI ScaI SfiI SgfI SgrAI
SnaBI SpeI SphI SwaI TaqII TstI Tth111I XbaI
XhoI XmnI