(Circular) (Six-base) MAPSORT of: pFN21ASDA0293.seq Check: 8363 from: 1 to: 9187 pFN21ASDA0293 9187 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3173 3173 Size: 9187 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2477 6997 278 Size: 53 83 186 936 941 4520 2468 Fragments arranged by size: 4520 2468 941 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4710 6530 1937 Size: 2773 1820 4594 Fragments arranged by size: 4594 2773 1820 Acc65I G'GTAC_C Cuts at: 6508 6508 Size: 9187 AclI AA'CG_TT Cuts at: 1563 7314 7687 1563 Size: 5751 373 3063 Fragments arranged by size: 5751 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2984 3216 4670 5333 5837 7250 8634 8902 Size: 1352 232 1454 663 504 1413 1384 268 Cuts at: 8902 1632 Size: 1917 Fragments arranged by size: 1917 1454 1413 1384 1352 663 504 268 232 AfeI AGC'GCT Cuts at: 2661 2661 Size: 9187 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8990 AflIII A'CryG_T Cuts at: 1203 4695 5166 8086 9063 1203 Size: 3492 471 2920 977 1327 Fragments arranged by size: 3492 2920 1327 977 471 AgeI A'CCGG_T Cuts at: 8018 8018 Size: 9187 AhdI GACnn_n'nnGTC Cuts at: 3385 4825 5278 5455 7916 3385 Size: 1440 453 177 2461 4656 Fragments arranged by size: 4656 2461 1440 453 177 AleI CACnn'nnGTG Cuts at: 1166 6363 1166 Size: 5197 3990 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4953 6381 1184 Size: 3769 1428 3990 Fragments arranged by size: 3990 3769 1428 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6461 6493 6461 Size: 32 9155 AlwNI CAG_nnn'CTG Cuts at: 3001 3165 3409 3581 3721 3868 4153 5003 5227 Size: 164 244 172 140 147 285 850 224 Cuts at: 5227 5490 5509 6930 8502 3001 Size: 263 19 1421 1572 3686 Fragments arranged by size: 3686 1572 1421 850 285 263 244 224 172 164 147 140 19 ApaI G_GGCC'C Cuts at: 2490 2874 2912 3419 3660 4246 4283 5864 6066 Size: 384 38 507 241 586 37 1581 202 Cuts at: 6066 2490 Size: 5611 Fragments arranged by size: 5611 1581 586 507 384 241 202 38 37 ApaLI G'TGCA_C Cuts at: 7244 8400 7244 Size: 1156 8031 ApoI r'AATT_y Cuts at: 1493 2174 3242 6496 6722 6874 8983 1493 Size: 681 1068 3254 226 152 2109 1697 Fragments arranged by size: 3254 2109 1697 1068 681 226 152 AseI AT'TA_AT Cuts at: 160 1032 2309 7741 160 Size: 872 1277 5432 1606 Fragments arranged by size: 5432 1606 1277 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9187 AvaI C'yCGr_G Cuts at: 1870 1943 2438 2525 2905 3398 3659 3934 4523 Size: 73 495 87 380 493 261 275 589 Cuts at: 4523 4676 5156 5535 5552 6059 6512 1870 Size: 153 480 379 17 507 453 4545 Fragments arranged by size: 4545 589 507 495 493 480 453 380 379 275 261 153 87 73 17 BamHI G'GATC_C Cuts at: 6080 6517 6884 6080 Size: 437 367 8383 Fragments arranged by size: 8383 437 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3560 4171 4189 4291 4937 Size: 325 217 627 1773 611 18 102 646 Cuts at: 4937 5548 6299 6508 7963 618 Size: 611 751 209 1455 1842 Fragments arranged by size: 1842 1773 1455 751 646 627 611 611 325 217 209 102 18 BanII G_rGCy'C Cuts at: 1391 2445 2490 2874 2912 2990 3419 3627 3660 Size: 1054 45 384 38 78 429 208 33 Cuts at: 3660 3782 3846 4032 4246 4283 4578 4725 4875 Size: 122 64 186 214 37 295 147 150 Cuts at: 4875 5015 5264 5622 5804 5864 6066 6506 1391 Size: 140 249 358 182 60 202 440 4072 Fragments arranged by size: 4072 1054 440 429 384 358 295 249 214 208 202 186 182 150 147 140 122 78 64 60 45 38 37 33 BbeI G_GCGC'C Cuts at: 3564 4175 4295 6303 3564 Size: 611 120 2008 6448 Fragments arranged by size: 6448 2008 611 120 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3204 3291 3384 928 Size: 971 1305 87 93 6731 Fragments arranged by size: 6731 1305 971 93 87 BbvCI CC'TCA_GC Cuts at: 2447 2781 3348 4584 5378 5943 6271 2447 Size: 334 567 1236 794 565 328 5363 Fragments arranged by size: 5363 1236 794 567 565 334 328 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7378 7412 1903 Size: 34 5441 34 3678 Fragments arranged by size: 5441 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7079 8289 7079 Size: 1210 7977 BclI T'GATC_A Cuts at: 1548 2745 1548 Size: 1197 7990 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2485 2517 2930 Size: 107 122 71 631 605 813 32 413 Cuts at: 2930 3590 4946 7798 136 Size: 660 1356 2852 1525 Fragments arranged by size: 2852 1525 1356 813 660 631 605 413 122 107 71 32 BglII A'GATC_T Cuts at: 9182 9182 Size: 9187 BlpI GC'TnA_GC Cuts at: 3128 4052 4409 5306 6604 3128 Size: 924 357 897 1298 5711 Fragments arranged by size: 5711 1298 924 897 357 Bme1580I G_kGCm'C Cuts at: 1790 2490 2874 2912 3326 3419 3660 4194 4246 Size: 700 384 38 414 93 241 534 52 Cuts at: 4246 4283 4521 5483 5864 6066 7248 8404 1790 Size: 37 238 962 381 202 1182 1156 2573 Fragments arranged by size: 2573 1182 1156 962 700 534 414 384 381 241 238 202 93 52 38 37 BmgBI CAC'GTC Cuts at: 1318 2971 1318 Size: 1653 7534 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2385 2630 2868 Size: 416 53 83 399 24 968 245 238 Cuts at: 2868 3091 3329 3620 4396 6357 6918 7876 442 Size: 223 238 291 776 1961 561 958 1753 Fragments arranged by size: 1961 1753 968 958 776 561 416 399 291 245 238 238 223 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9187 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3976 5029 5092 5641 3976 Size: 1053 63 549 7522 Fragments arranged by size: 7522 1053 549 63 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2066 2780 2939 2966 3023 3056 3074 3142 Size: 827 714 159 27 57 33 18 68 Cuts at: 3142 3272 4133 4178 4739 5060 5062 5417 5867 Size: 130 861 45 561 321 2 355 450 Cuts at: 5867 6215 6369 6488 7847 1239 Size: 348 154 119 1359 2579 Fragments arranged by size: 2579 1359 861 827 714 561 450 355 348 321 159 154 130 119 68 57 45 33 27 18 2 Bpu10I CC'TnA_GC Cuts at: 2447 2781 3348 3603 3621 3896 4584 5378 5510 Size: 334 567 255 18 275 688 794 132 Cuts at: 5510 5943 6271 2447 Size: 433 328 5363 Fragments arranged by size: 5363 794 688 567 433 334 328 275 255 132 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4562 5627 7312 8177 8475 4562 Size: 1065 1685 865 298 5274 Fragments arranged by size: 5274 1685 1065 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2403 7850 882 Size: 617 904 5447 2219 Fragments arranged by size: 5447 2219 904 617 BsaAI yAC'GTr Cuts at: 493 1204 3760 4636 5169 6484 6978 493 Size: 711 2556 876 533 1315 494 2702 Fragments arranged by size: 2702 2556 1315 876 711 533 494 BsaBI GATnn'nnATC Cuts at: 9181 9181 Size: 9187 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2474 3561 3969 4095 Size: 53 83 186 936 941 1087 408 126 Cuts at: 4095 4172 4292 4365 6300 6994 7376 8011 275 Size: 77 120 73 1935 694 382 635 1451 Fragments arranged by size: 1935 1451 1087 941 936 694 635 408 382 186 126 120 83 77 73 53 BsaWI w'CCGG_w Cuts at: 7620 8018 8292 8439 8850 7620 Size: 398 274 147 411 7957 Fragments arranged by size: 7957 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2064 2094 3995 4025 4376 Size: 30 165 30 661 30 1901 30 351 Cuts at: 4376 4406 5156 5186 6461 6491 1178 Size: 30 750 30 1275 30 3874 Fragments arranged by size: 3874 1901 1275 750 661 351 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2144 2435 2648 2726 3020 3683 3686 4280 Size: 958 291 213 78 294 663 3 594 Cuts at: 4280 4438 4441 4444 4447 5074 5570 5612 5882 Size: 158 3 3 3 627 496 42 270 Cuts at: 5882 5948 6026 6217 6256 6259 6473 1186 Size: 66 78 191 39 3 214 3900 Fragments arranged by size: 3900 958 663 627 594 496 294 291 270 214 213 191 158 78 78 66 42 39 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2378 2790 3260 3392 3412 3419 3467 3502 3547 Size: 412 470 132 20 7 48 35 45 Cuts at: 3547 3763 4792 4988 5483 8390 8886 2378 Size: 216 1029 196 495 2907 496 2679 Fragments arranged by size: 2907 2679 1029 496 495 470 412 216 196 132 48 45 35 20 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2103 2993 4346 1163 Size: 573 367 890 1353 6004 Fragments arranged by size: 6004 1353 890 573 367 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4399 4486 4599 4835 6222 6637 Size: 275 186 2398 87 113 236 1387 415 Cuts at: 6637 7398 8426 1540 Size: 761 1028 2301 Fragments arranged by size: 2398 2301 1387 1028 761 415 275 236 186 113 87 BsiHKAI G_wGCw'C Cuts at: 2445 2906 2990 4578 4725 5015 5622 7248 7333 Size: 461 84 1588 147 290 607 1626 85 Cuts at: 7333 8404 2445 Size: 1071 3228 Fragments arranged by size: 3228 1626 1588 1071 607 461 290 147 85 84 BsmI GAATG_Cn' Cuts at: 2520 3981 6698 6791 2520 Size: 1461 2717 93 4916 Fragments arranged by size: 4916 2717 1461 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2445 2490 2874 2906 2912 2990 3326 Size: 399 655 45 384 32 6 78 336 Cuts at: 3326 3419 3627 3660 3782 3846 4032 4194 4246 Size: 93 208 33 122 64 186 162 52 Cuts at: 4246 4283 4521 4578 4725 4875 5015 5264 5483 Size: 37 238 57 147 150 140 249 219 Cuts at: 5483 5622 5804 5864 6066 6506 7248 7333 8404 Size: 139 182 60 202 440 742 85 1071 Cuts at: 8404 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 655 440 399 384 336 249 238 219 208 202 186 182 162 150 147 140 139 122 93 85 78 64 60 57 52 45 37 33 32 6 BspEI T'CCGG_A Cuts at: 8850 8850 Size: 9187 BspHI T'CATG_A Cuts at: 2748 6240 7076 8806 2748 Size: 3492 836 1730 3129 Fragments arranged by size: 3492 3129 1730 836 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3173 5283 6542 844 Size: 2329 2110 1259 3489 Fragments arranged by size: 3489 2329 2110 1259 BsrBI CCG'CTC Cuts at: 1394 1823 3040 3650 4185 4210 4297 5076 5094 Size: 429 1217 610 535 25 87 779 18 Cuts at: 5094 5472 6442 7074 1394 Size: 378 970 632 3507 Fragments arranged by size: 3507 1217 970 779 632 610 535 429 378 87 25 18 BsrDI GCAATG_nn' Cuts at: 65 1248 7682 7856 65 Size: 1183 6434 174 1396 Fragments arranged by size: 6434 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4367 5441 7831 8018 1700 Size: 110 2557 1074 2390 187 2869 Fragments arranged by size: 2869 2557 2390 1074 187 110 BsrGI T'GTAC_A Cuts at: 96 4692 96 Size: 4596 4591 BssHII G'CGCG_C Cuts at: 1925 4608 1925 Size: 2683 6504 BssSI C'ACGA_G Cuts at: 7247 8259 7247 Size: 1012 8175 BstAPI GCAn_nnn'nTGC Cuts at: 794 3409 3760 4153 794 Size: 2615 351 393 5828 Fragments arranged by size: 5828 2615 393 351 BstBI TT'CG_AA Cuts at: 2007 7991 2007 Size: 5984 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9187 BstXI CCAn_nnnn'nTGG Cuts at: 2848 3151 3268 4934 2848 Size: 303 117 1666 7101 Fragments arranged by size: 7101 1666 303 117 BstYI r'GATC_y Cuts at: 1972 2949 3792 4773 6080 6517 6884 7269 7286 Size: 977 843 981 1307 437 367 385 17 Cuts at: 7286 8738 8824 8836 9182 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1307 981 977 843 437 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2193 3279 5865 2193 Size: 1086 2586 5515 Fragments arranged by size: 5515 2586 1086 BtgI C'CryG_G Cuts at: 513 1065 2022 3627 4399 4498 4841 513 Size: 552 957 1605 772 99 343 4859 Fragments arranged by size: 4859 1605 957 772 552 343 99 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4042 487 Size: 683 56 2816 5632 Fragments arranged by size: 5632 2816 683 56 BtsI GCAGTG_nn' Cuts at: 2990 3203 4926 5567 6333 6703 7497 7517 2990 Size: 213 1723 641 766 370 794 20 4660 Fragments arranged by size: 4660 1723 794 766 641 370 213 20 ClaI AT'CG_AT Cuts at: 1554 2042 1554 Size: 488 8699 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9152 DraI TTT'AAA Cuts at: 6491 6491 Size: 9187 DraIII CAC_nnn'GTG Cuts at: 2424 2901 4127 2424 Size: 477 1226 7484 Fragments arranged by size: 7484 1226 477 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8194 809 Size: 507 6878 1802 Fragments arranged by size: 6878 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2613 3051 Size: 54 1425 50 275 18 195 588 438 Cuts at: 3051 3186 3270 4211 4221 4471 4483 4596 4749 Size: 135 84 941 10 250 12 113 153 Cuts at: 4749 4832 4912 5145 5463 6219 6408 6634 7523 Size: 83 80 233 318 756 189 226 889 Cuts at: 7523 9152 8 Size: 1629 43 Fragments arranged by size: 1629 1425 941 889 756 588 438 318 275 250 233 226 195 189 153 135 113 84 83 80 54 50 43 18 12 10 EagI C'GGCC_G Cuts at: 1537 1812 4483 4596 4832 6219 6634 1537 Size: 275 2671 113 236 1387 415 4090 Fragments arranged by size: 4090 2671 1387 415 275 236 113 EarI CTCTTCn'nnn_ Cuts at: 1353 2423 3075 5003 5943 7117 1353 Size: 1070 652 1928 940 1174 3423 Fragments arranged by size: 3423 1928 1174 1070 940 652 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2156 2291 2585 2713 2834 3004 Size: 1064 138 567 135 294 128 121 170 Cuts at: 3004 3660 4562 7774 8148 8294 387 Size: 656 902 3212 374 146 1280 Fragments arranged by size: 3212 1280 1064 902 656 567 374 294 170 146 138 135 128 121 EcoICRI GAG'CTC Cuts at: 2443 2988 4576 4723 5013 5620 2443 Size: 545 1588 147 290 607 6010 Fragments arranged by size: 6010 1588 607 545 290 147 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 2780 2939 2966 2984 3023 3056 Size: 393 434 714 159 27 18 39 33 Cuts at: 3056 3074 3142 3216 3272 4133 4178 4670 4739 Size: 18 68 74 56 861 45 492 69 Cuts at: 4739 5060 5062 5333 5417 5837 5867 6215 6369 Size: 321 2 271 84 420 30 348 154 Cuts at: 6369 6488 7250 7847 8634 8902 1239 Size: 119 762 597 787 268 1524 Fragments arranged by size: 1524 861 787 762 714 597 492 434 420 393 348 321 271 268 159 154 119 84 74 69 68 56 45 39 33 30 27 18 18 2 EcoNI CCTnn'n_nnAGG Cuts at: 3176 3366 3954 4115 4802 3176 Size: 190 588 161 687 7561 Fragments arranged by size: 7561 687 588 190 161 EcoO109I rG'GnC_Cy Cuts at: 2584 2870 2909 3416 3428 3656 4242 4243 4279 Size: 286 39 507 12 228 586 1 36 Cuts at: 4279 4280 4510 4968 5119 5197 5506 5682 5715 Size: 1 230 458 151 78 309 176 33 Cuts at: 5715 5750 5860 5861 5999 6063 6463 2584 Size: 35 110 1 138 64 400 5308 Fragments arranged by size: 5308 586 507 458 400 309 286 230 228 176 151 138 110 78 64 39 36 35 33 12 1 1 1 EcoRI G'AATT_C Cuts at: 6496 6874 6496 Size: 378 8809 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2908 3241 3862 2908 Size: 333 621 8233 Fragments arranged by size: 8233 621 333 FspI TGC'GCA Cuts at: 7693 7693 Size: 9187 HaeII r_GCGC'y Cuts at: 1678 2663 3564 3896 4154 4175 4295 6303 8334 Size: 985 901 332 258 21 120 2008 2031 Cuts at: 8334 1678 Size: 2531 Fragments arranged by size: 2531 2031 2008 985 901 332 258 120 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2200 2232 2459 2491 2705 Size: 32 135 32 628 32 227 32 214 Cuts at: 2705 2737 2996 3028 3371 3403 3912 3944 3995 Size: 32 259 32 343 32 509 32 51 Cuts at: 3995 4027 4199 4231 4811 4843 5156 5188 5264 Size: 32 172 32 580 32 313 32 76 Cuts at: 5264 5296 5441 5473 6083 6115 6397 6429 7828 Size: 32 145 32 610 32 282 32 1399 Cuts at: 7828 7860 7902 7934 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1399 628 610 580 509 343 313 282 259 227 214 172 145 135 76 51 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2833 6531 6777 669 Size: 988 281 895 3698 246 3079 Fragments arranged by size: 3698 3079 988 895 281 246 HindIII A'AGCT_T Cuts at: 748 2230 748 Size: 1482 7705 HpaI GTT'AAC Cuts at: 6777 6777 Size: 9187 KasI G'GCGC_C Cuts at: 3560 4171 4291 6299 3560 Size: 611 120 2008 6448 Fragments arranged by size: 6448 2008 611 120 KpnI G_GTAC'C Cuts at: 6512 6512 Size: 9187 MfeI C'AATT_G Cuts at: 6786 6786 Size: 9187 MluI A'CGCG_T Cuts at: 9063 9063 Size: 9187 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3852 4419 8301 8485 1305 Size: 2547 567 3882 184 2007 Fragments arranged by size: 3882 2547 2007 567 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3188 3272 4223 4473 4751 Size: 54 1425 343 1356 84 951 250 278 Cuts at: 4751 5147 6410 10 Size: 396 1263 2787 Fragments arranged by size: 2787 1425 1356 1263 951 396 343 278 250 84 54 MslI CAynn'nnrTG Cuts at: 518 1166 3854 3908 4883 6363 7145 7504 7663 Size: 648 2688 54 975 1480 782 359 159 Cuts at: 7663 518 Size: 2042 Fragments arranged by size: 2688 2042 1480 975 782 648 359 159 54 MspA1I CmG'CkG Cuts at: 1606 2794 2821 3629 3679 3718 3742 4051 4275 Size: 1188 27 808 50 39 24 309 224 Cuts at: 4275 4401 4500 4669 4891 5224 5344 5587 6391 Size: 126 99 169 222 333 120 243 804 Cuts at: 6391 6603 7280 8428 8673 1606 Size: 212 677 1148 245 2120 Fragments arranged by size: 2120 1188 1148 808 804 677 333 309 245 243 224 222 212 169 126 120 99 50 39 27 24 NaeI GCC'GGC Cuts at: 4369 4369 Size: 9187 NarI GG'CG_CC Cuts at: 3561 4172 4292 6300 3561 Size: 611 120 2008 6448 Fragments arranged by size: 6448 2008 611 120 NcoI C'CATG_G Cuts at: 513 1065 2022 4841 513 Size: 552 957 2819 4859 Fragments arranged by size: 4859 2819 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9187 NgoMIV G'CCGG_C Cuts at: 4367 4367 Size: 9187 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9187 NotI GC'GGCC_GC Cuts at: 6634 6634 Size: 9187 NspI r_CATG'y Cuts at: 1132 1229 4699 4825 4888 5341 6545 8090 1132 Size: 97 3470 126 63 453 1204 1545 2229 Fragments arranged by size: 3470 2229 1545 1204 453 126 97 63 PasI CC'CwG_GG Cuts at: 1348 2866 3178 4176 5123 1348 Size: 1518 312 998 947 5412 Fragments arranged by size: 5412 1518 998 947 312 PciI A'CATG_T Cuts at: 4695 8086 4695 Size: 3391 5796 PflMI CCAn_nnn'nTGG Cuts at: 1108 3184 3813 4921 5295 5368 1108 Size: 2076 629 1108 374 73 4927 Fragments arranged by size: 4927 2076 1108 629 374 73 PfoI T'CCnGG_A Cuts at: 2811 4110 5133 5684 5752 2811 Size: 1299 1023 551 68 6246 Fragments arranged by size: 6246 1299 1023 551 68 PmeI GTTT'AAAC Cuts at: 6491 6491 Size: 9187 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6200 6232 6461 6493 7256 7288 8769 8801 6200 Size: 32 229 32 763 32 1481 32 6586 Fragments arranged by size: 6586 1481 763 229 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4968 5197 5506 5682 5715 5750 5999 4968 Size: 229 309 176 33 35 249 8156 Fragments arranged by size: 8156 309 249 229 176 35 33 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9187 PsiI TTA'TAA Cuts at: 6757 6757 Size: 9187 PspOMI G'GGCC_C Cuts at: 2486 2870 2908 3415 3656 4242 4279 5860 6062 Size: 384 38 507 241 586 37 1581 202 Cuts at: 6062 2486 Size: 5611 Fragments arranged by size: 5611 1581 586 507 384 241 202 38 37 PstI C_TGCA'G Cuts at: 830 2373 2559 2997 3170 5499 5523 6191 6539 Size: 1543 186 438 173 2329 24 668 348 Cuts at: 6539 830 Size: 3478 Fragments arranged by size: 3478 2329 1543 668 438 348 186 173 24 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9187 PvuII CAG'CTG Cuts at: 2821 3679 3718 4891 5344 5587 2821 Size: 858 39 1173 453 243 6421 Fragments arranged by size: 6421 1173 858 453 243 39 SacI G_AGCT'C Cuts at: 2445 2990 4578 4725 5015 5622 2445 Size: 545 1588 147 290 607 6010 Fragments arranged by size: 6010 1588 607 545 290 147 SacII CC_GC'GG Cuts at: 3630 4402 4501 3630 Size: 772 99 8316 Fragments arranged by size: 8316 772 99 SalI G'TCGA_C Cuts at: 1936 6529 1936 Size: 4593 4594 SanDI GG'GwC_CC Cuts at: 5715 5715 Size: 9187 SapI GCTCTTCn'nnn_ Cuts at: 5003 5003 Size: 9187 SbfI CC_TGCA'GG Cuts at: 6539 6539 Size: 9187 ScaI AGT'ACT Cuts at: 7435 9147 7435 Size: 1712 7475 SfcI C'TryA_G Cuts at: 826 1045 2369 2555 2993 3166 5495 5519 5677 Size: 219 1324 186 438 173 2329 24 158 Cuts at: 5677 6187 6535 7670 8351 8542 826 Size: 510 348 1135 681 191 1471 Fragments arranged by size: 2329 1471 1324 1135 681 510 438 348 219 191 186 173 158 24 SfiI GGCCn_nnn'nGGCC Cuts at: 2930 2930 Size: 9187 SfoI GGC'GCC Cuts at: 3562 4173 4293 6301 3562 Size: 611 120 2008 6448 Fragments arranged by size: 6448 2008 611 120 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9187 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9187 SmaI CCC'GGG Cuts at: 1872 2527 3400 3661 5537 6061 6514 1872 Size: 655 873 261 1876 524 453 4545 Fragments arranged by size: 4545 1876 873 655 524 453 261 SmlI C'TyrA_G Cuts at: 820 1017 1943 4577 5642 7291 8192 8454 820 Size: 197 926 2634 1065 1649 901 262 1553 Fragments arranged by size: 2634 1649 1553 1065 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6484 6978 493 Size: 5991 494 2702 Fragments arranged by size: 5991 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 9187 SphI G_CATG'C Cuts at: 1132 4888 5341 6545 1132 Size: 3756 453 1204 3774 Fragments arranged by size: 3774 3756 1204 453 SspI AAT'ATT Cuts at: 5 52 2054 7111 5 Size: 47 2002 5057 2081 Fragments arranged by size: 5057 2081 2002 47 StuI AGG'CCT Cuts at: 1517 3224 5579 6237 1517 Size: 1707 2355 658 4467 Fragments arranged by size: 4467 2355 1707 658 StyI C'CwwG_G Cuts at: 513 1065 2022 2724 2937 4251 4841 4964 5995 Size: 552 957 702 213 1314 590 123 1031 Cuts at: 5995 6369 6626 513 Size: 374 257 3074 Fragments arranged by size: 3074 1314 1031 957 702 590 552 374 257 213 123 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2513 3772 5796 5906 7214 7231 7384 Size: 1255 933 1259 2024 110 1308 17 153 Cuts at: 7384 7569 8014 325 Size: 185 445 1498 Fragments arranged by size: 2024 1498 1308 1259 1255 933 445 185 153 110 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2596 3886 4692 Size: 274 80 33 51 1443 619 1290 806 Cuts at: 4692 5103 7433 9145 96 Size: 411 2330 1712 138 Fragments arranged by size: 2330 1712 1443 1290 806 619 411 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4376 4408 9147 9179 4376 Size: 32 4739 32 4384 Fragments arranged by size: 4739 4384 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9187 XbaI T'CTAG_A Cuts at: 3985 6523 3985 Size: 2538 6649 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2873 3073 4039 6340 2873 Size: 200 966 2301 5720 Fragments arranged by size: 5720 2301 966 200 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9187 XmaI C'CCGG_G Cuts at: 1870 2525 3398 3659 5535 6059 6512 1870 Size: 655 873 261 1876 524 453 4545 Fragments arranged by size: 4545 1876 873 655 524 453 261 XmnI GAAnn'nnTTC Cuts at: 3863 7316 3863 Size: 3453 5734 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2475 6995 276 Size: 53 83 186 936 941 4520 2468 Fragments arranged by size: 4520 2468 941 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SexAI SrfI SwaI