Restriction map for
pFN21ASDA0290
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# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AgeI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BamHI BcgI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaWI
BseAI BsiWI BsmI BspDI BspEI
BspHI BssHII BssSI Bst1107I BstBI
BstZ17I CelII ClaI CpoI DraI
Eco47III EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI MamI MfeI MluI
MroI MunI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshBI PvuI RcaI RsrII
SacI SalI ScaI SfiI SfuI
SgrAI SnaBI SpeI SphI SspI
SwaI Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AclI 842 AACGTT AA^CGTT
AcsI 703 GAATTT [GA]^AATT[CT]
Alw44I 1075 GTGCAC G^TGCAC
AocI 621 CCTGAGG CC^T[ACGT]AGG
ApaLI 1075 GTGCAC G^TGCAC
ApoI 703 GAATTT [GA]^AATT[CT]
BbeI 1518 GGCGCC GGCGC^C
BbrPI 676 CACGTG CAC^GTG
BbsI 217 GTCTTC GAAGAC(2/6)
BbvCI 2025 CCTCAGC CCTCAGC(-5/-2)
BciVI 1541 GTATCC GTATCC(6/5)
BglI 1175 GCCCAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 217 GTCTTC GAAGAC(2/6)
BsiEI 1007 CGGCCG CG[GA][CT]^CG
Bsp1407I 2040 TGTACA T^GTACA
BspLU11I 946 ACATGT A^CATGT
BsrDI 1727 GCAATG GCAATG(2/0)
BsrGI 2040 TGTACA T^GTACA
BstEII 2608 GGTTACC G^GT[ACGT]ACC
BstPI 2608 GGTTACC G^GT[ACGT]ACC
Bsu36I 621 CCTGAGG CC^T[ACGT]AGG
DrdI 2568 GACCACTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1007 CGGCCG C^GGCCG
EclXI 1007 CGGCCG C^GGCCG
Eco52I 1007 CGGCCG C^GGCCG
Eco81I 621 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2608 GGTTACC G^GT[ACGT]ACC
KasI 1518 GGCGCC G^GCGCC
KspI 1148 CCGCGG CCGC^GG
MseI 707 TTAA T^TAA
NarI 1518 GGCGCC GG^CGCC
NdeI 650 CATATG CA^TATG
PaeR7I 1946 CTCGAG C^TCGAG
PflMI 600 CCAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 676 CACGTG CAC^GTG
PmlI 676 CACGTG CAC^GTG
Psp1406I 842 AACGTT AA^CGTT
SacII 1148 CCGCGG CCGC^GG
SbfI 825 CCTGCAGG CCTGCA^GG
SexAI 2654 ACCTGGT A^CC[AT]GGT
SfoI 1518 GGCGCC GGC^GCC
Sse8387I 825 CCTGCAGG CCTGCA^GG
SspBI 2040 TGTACA T^GTACA
Tru9I 707 TTAA T^TAA
Tsp509I 704 AATT ^AATT
Van91I 600 CCAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1946 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AcyI 385 GGCGTC G[GA]^CG[CT]C
1518 GGCGCC
AflIII 946 ACATGT A^C[GA][CT]GT
1720 ACACGT
AhdI 1432 GACTCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1823 GACACGGAGTC
AlwI 2367 GATCC GGATC(4/5)
279 GGATC
ApaI 76 GGGCCC GGGCC^C
2521 GGGCCC
AspEI 1432 GACTCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1823 GACACGGAGTC
BalI 170 TGGCCA TGG^CCA
1923 TGGCCA
BfaI 1355 CTAG C^TAG
1697 CTAG
BsaAI 676 CACGTG [CT]AC^GT[GA]
1721 CACGTA
BsaHI 385 GGCGTC G[GA]^CG[CT]C
1518 GGCGCC
BsmBI 225 CGTCTC CGTCTC(1/5)
1586 CGTCTC
BstAPI 570 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
808 GCATACAGTGC
BstYI 279 GGATCT [GA]^GATC[CT]
2366 AGATCC
DraIII 61 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
658 CACTCGGTG
Eam1105I 1432 GACTCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1823 GACACGGAGTC
Hin1I 385 GGCGTC G[GA]^CG[CT]C
1518 GGCGCC
MaeI 1355 CTAG C^TAG
1697 CTAG
MflI 279 GGATCT [GA]^GATC[CT]
2366 AGATCC
MluNI 170 TGGCCA TGG^CCA
1923 TGGCCA
MscI 170 TGGCCA TGG^CCA
1923 TGGCCA
NaeI 884 GCCGGC GCC^GGC
2412 GCCGGC
NcoI 188 CCATGG C^CATGG
605 CCATGG
NgoMIV 884 GCCGGC G^CCGGC
2412 GCCGGC
NspI 946 ACATGT [GA]CATG^[CT]
2615 GCATGT
PshAI 1232 GACCTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2394 GACCAACGTC
SapI 1054 GAAGAGC GCTCTTC(1/4)
1769 GCTCTTC
SmlI 319 CTCAAG C^T[CT][GA]AG
1946 CTCGAG
StuI 860 AGGCCT AGG^CCT
1610 AGGCCT
XhoII 279 GGATCT [GA]^GATC[CT]
2366 AGATCC
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# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
XcmI XmaCI XmaI