Restriction map for
pFN21ASDA0290
(Linear) (Six-base) MAPSORT of: KIAA0290.seq Check: 9944 from: 1 to: 2667
KIAA0290.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 681 918 1278 1650 2667
Size: 681 237 360 372 1017
Fragments arranged by size:
1017 681 372 360 237
AclI AA'CG_TT
Cuts at: 0 843 2667
Size: 843 1824
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1757 2667
Size: 1757 910
AflIII A'CryG_T
Cuts at: 0 946 1720 2667
Size: 946 774 947
Fragments arranged by size:
947 946 774
AhdI GACnn_n'nnGTC
Cuts at: 0 1437 1828 2667
Size: 1437 391 839
Fragments arranged by size:
1437 839 391
AleI CACnn'nnGTG
Cuts at: 0 676 2338 2667
Size: 676 1662 329
Fragments arranged by size:
1662 676 329
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2282 2432 2667
Size: 2282 150 235
Fragments arranged by size:
2282 235 150
AlwNI CAG_nnn'CTG
Cuts at: 0 399 1259 2370 2667
Size: 399 860 1111 297
Fragments arranged by size:
1111 860 399 297
ApaI G_GGCC'C
Cuts at: 0 80 2525 2667
Size: 80 2445 142
Fragments arranged by size:
2445 142 80
ApaLI G'TGCA_C
Cuts at: 0 1075 2667
Size: 1075 1592
ApoI r'AATT_y
Cuts at: 0 703 2667
Size: 703 1964
AvaI C'yCGr_G
Cuts at: 0 381 554 1097 1117 1835 1946 2235 2667
Size: 381 173 543 20 718 111 289 432
Fragments arranged by size:
718 543 432 381 289 173 111 20
BanI G'GyrC_C
Cuts at: 0 1183 1449 1489 1518 1548 2329 2667
Size: 1183 266 40 29 30 781 338
Fragments arranged by size:
1183 781 338 266 40 30 29
BanII G_rGCy'C
Cuts at: 0 80 649 1016 1061 1104 1504 1756 1771
Size: 80 569 367 45 43 400 252 15
Cuts at: 1771 1849 1864 2525 2667
Size: 78 15 661 142
Fragments arranged by size:
661 569 400 367 252 142 80 78
45 43 15 15
BbeI G_GCGC'C
Cuts at: 0 1522 2667
Size: 1522 1145
BbsI GAAGACnn'nnnn_
Cuts at: 0 210 2667
Size: 210 2457
BbvCI CC'TCA_GC
Cuts at: 0 2026 2667
Size: 2026 641
BciVI GTATCCnnnnn_n'
Cuts at: 0 1552 2667
Size: 1552 1115
BglI GCCn_nnn'nGGC
Cuts at: 0 1181 2667
Size: 1181 1486
Bme1580I G_kGCm'C
Cuts at: 0 80 772 1079 1186 1551 2271 2345 2525
Size: 80 692 307 107 365 720 74 180
Cuts at: 2525 2667
Size: 142
Fragments arranged by size:
720 692 365 307 180 142 107 80
74
BmrI ACTGGGnnnn_n'
Cuts at: 0 1774 1964 2238 2667
Size: 1774 190 274 429
Fragments arranged by size:
1774 429 274 190
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 156 2308 2532 2667
Size: 156 2152 224 135
Fragments arranged by size:
2152 224 156 135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 949 1088 1339 1359 1574 1627 2452 2667
Size: 949 139 251 20 215 53 825 215
Fragments arranged by size:
949 825 251 215 215 139 53 20
Bpu10I CC'TnA_GC
Cuts at: 0 1138 1750 2026 2667
Size: 1138 612 276 641
Fragments arranged by size:
1138 641 612 276
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 2667
Size: 304 2363
BsaI GGTCTCn'nnnn_
Cuts at: 0 127 1224 1619 2110 2307 2667
Size: 127 1097 395 491 197 360
Fragments arranged by size:
1097 491 395 360 197 127
BsaAI yAC'GTr
Cuts at: 0 678 1723 2667
Size: 678 1045 944
Fragments arranged by size:
1045 944 678
BsaHI Gr'CG_yC
Cuts at: 0 386 1519 2667
Size: 386 1133 1148
Fragments arranged by size:
1148 1133 386
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 447 477 1276 1306 1816 1846 2212 2242
Size: 447 30 799 30 510 30 366 30
Cuts at: 2242 2667
Size: 425
Fragments arranged by size:
799 510 447 425 366 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 145 219 625 1297 1383 1386 1637 2449
Size: 145 74 406 672 86 3 251 812
Cuts at: 2449 2646 2667
Size: 197 21
Fragments arranged by size:
812 672 406 251 197 145 86 74
21 3
BseYI C'CCAG_C
Cuts at: 0 642 1216 1507 1801 1933 2504 2524 2533
Size: 642 574 291 294 132 571 20 9
Cuts at: 2533 2667
Size: 134
Fragments arranged by size:
642 574 571 294 291 134 132 20
9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 700 1283 1622 2531 2667
Size: 391 309 583 339 909 136
Fragments arranged by size:
909 583 391 339 309 136
BsiEI CG_ry'CG
Cuts at: 0 1010 2667
Size: 1010 1657
BsiHKAI G_wGCw'C
Cuts at: 0 358 1079 2063 2667
Size: 358 721 984 604
Fragments arranged by size:
984 721 604 358
BsmBI CGTCTCn'nnnn_
Cuts at: 0 231 1592 2667
Size: 231 1361 1075
Fragments arranged by size:
1361 1075 231
Bsp1286I G_dGCh'C
Cuts at: 0 80 358 649 772 1016 1061 1079 1104
Size: 80 278 291 123 244 45 18 25
Cuts at: 1104 1186 1504 1551 1756 1771 1849 1864 2063
Size: 82 318 47 205 15 78 15 199
Cuts at: 2063 2271 2345 2525 2667
Size: 208 74 180 142
Fragments arranged by size:
318 291 278 244 208 205 199 180
142 123 82 80 78 74 47 45
25 18 15 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 363 681 918 1278 1650 2667
Size: 363 318 237 360 372 1017
Fragments arranged by size:
1017 372 363 360 318 237
BsrBI CCG'CTC
Cuts at: 0 207 447 894 2514 2667
Size: 207 240 447 1620 153
Fragments arranged by size:
1620 447 240 207 153
BsrDI GCAATG_nn'
Cuts at: 0 1734 2667
Size: 1734 933
BsrFI r'CCGG_y
Cuts at: 0 884 2321 2412 2667
Size: 884 1437 91 255
Fragments arranged by size:
1437 884 255 91
BsrGI T'GTAC_A
Cuts at: 0 2040 2667
Size: 2040 627
BstAPI GCAn_nnn'nTGC
Cuts at: 0 576 814 2667
Size: 576 238 1853
Fragments arranged by size:
1853 576 238
BstEII G'GTnAC_C
Cuts at: 0 2608 2667
Size: 2608 59
BstXI CCAn_nnnn'nTGG
Cuts at: 0 96 1472 1598 1941 2541 2667
Size: 96 1376 126 343 600 126
Fragments arranged by size:
1376 600 343 126 126 96
BstYI r'GATC_y
Cuts at: 0 279 2366 2667
Size: 279 2087 301
Fragments arranged by size:
2087 301 279
Bsu36I CC'TnA_GG
Cuts at: 0 622 2667
Size: 622 2045
BtgI C'CryG_G
Cuts at: 0 188 605 1148 1920 2333 2540 2667
Size: 188 417 543 772 413 207 127
Fragments arranged by size:
772 543 417 413 207 188 127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 163 850 2667
Size: 163 687 1817
Fragments arranged by size:
1817 687 163
BtsI GCAGTG_nn'
Cuts at: 0 637 1784 2186 2284 2667
Size: 637 1147 402 98 383
Fragments arranged by size:
1147 637 402 383 98
DraIII CAC_nnn'GTG
Cuts at: 0 66 663 2667
Size: 66 597 2004
Fragments arranged by size:
2004 597 66
DrdI GACnn_nn'nnGTC
Cuts at: 0 2574 2667
Size: 2574 93
EaeI y'GGCC_r
Cuts at: 0 170 1007 1616 1923 2487 2667
Size: 170 837 609 307 564 180
Fragments arranged by size:
837 609 564 307 180 170
EagI C'GGCC_G
Cuts at: 0 1007 2667
Size: 1007 1660
EarI CTCTTCn'nnn_
Cuts at: 0 1049 1776 2623 2667
Size: 1049 727 847 44
Fragments arranged by size:
1049 847 727 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 115 505 2667
Size: 115 390 2162
Fragments arranged by size:
2162 390 115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 949 1088 1339 1359 1574 1627 1757 2452
Size: 949 139 251 20 215 53 130 695
Cuts at: 2452 2667
Size: 215
Fragments arranged by size:
949 695 251 215 215 139 130 53
20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 938 1175 2296 2667
Size: 938 237 1121 371
Fragments arranged by size:
1121 938 371 237
EcoO109I rG'GnC_Cy
Cuts at: 0 51 76 182 194 1476 1594 1665 1734
Size: 51 25 106 12 1282 118 71 69
Cuts at: 1734 2006 2239 2521 2667
Size: 272 233 282 146
Fragments arranged by size:
1282 282 272 233 146 118 106 71
69 51 25 12
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1320 2667
Size: 1320 1347
HaeII r_GCGC'y
Cuts at: 0 247 531 1522 2169 2667
Size: 247 284 991 647 498
Fragments arranged by size:
991 647 498 284 247
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1276 1308 1423 1455 1814 1846 2667
Size: 1276 32 115 32 359 32 821
Fragments arranged by size:
1276 821 359 115 32 32 32
KasI G'GCGC_C
Cuts at: 0 1518 2667
Size: 1518 1149
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1064 2667
Size: 1064 1603
MscI TGG'CCA
Cuts at: 0 172 1925 2667
Size: 172 1753 742
Fragments arranged by size:
1753 742 172
MslI CAynn'nnrTG
Cuts at: 0 676 2338 2647 2667
Size: 676 1662 309 20
Fragments arranged by size:
1662 676 309 20
MspA1I CmG'CkG
Cuts at: 0 916 1129 1150 1259 1511 1937 2003 2504
Size: 916 213 21 109 252 426 66 501
Cuts at: 2504 2606 2667
Size: 102 61
Fragments arranged by size:
916 501 426 252 213 109 102 66
61 21
NaeI GCC'GGC
Cuts at: 0 886 2414 2667
Size: 886 1528 253
Fragments arranged by size:
1528 886 253
NarI GG'CG_CC
Cuts at: 0 1519 2667
Size: 1519 1148
NcoI C'CATG_G
Cuts at: 0 188 605 2667
Size: 188 417 2062
Fragments arranged by size:
2062 417 188
NdeI CA'TA_TG
Cuts at: 0 651 2667
Size: 651 2016
NgoMIV G'CCGG_C
Cuts at: 0 884 2412 2667
Size: 884 1528 255
Fragments arranged by size:
1528 884 255
NspI r_CATG'y
Cuts at: 0 950 2619 2667
Size: 950 1669 48
Fragments arranged by size:
1669 950 48
PasI CC'CwG_GG
Cuts at: 0 1177 1598 2667
Size: 1177 421 1069
Fragments arranged by size:
1177 1069 421
PciI A'CATG_T
Cuts at: 0 946 2667
Size: 946 1721
PflMI CCAn_nnn'nTGG
Cuts at: 0 606 2667
Size: 606 2061
PfoI T'CCnGG_A
Cuts at: 0 110 873 926 2667
Size: 110 763 53 1741
Fragments arranged by size:
1741 763 110 53
PmlI CAC'GTG
Cuts at: 0 678 2667
Size: 678 1989
PpuMI rG'GwC_Cy
Cuts at: 0 51 182 194 1476 1734 2006 2239 2667
Size: 51 131 12 1282 258 272 233 428
Fragments arranged by size:
1282 428 272 258 233 131 51 12
PshAI GACnn'nnGTC
Cuts at: 0 1236 2398 2667
Size: 1236 1162 269
Fragments arranged by size:
1236 1162 269
PspOMI G'GGCC_C
Cuts at: 0 76 2521 2667
Size: 76 2445 146
Fragments arranged by size:
2445 146 76
PspXI vC'TCGA_Gb
Cuts at: 0 1946 2667
Size: 1946 721
PstI C_TGCA'G
Cuts at: 0 830 915 1114 2165 2261 2667
Size: 830 85 199 1051 96 406
Fragments arranged by size:
1051 830 406 199 96 85
PvuII CAG'CTG
Cuts at: 0 916 1259 1511 1937 2504 2667
Size: 916 343 252 426 567 163
Fragments arranged by size:
916 567 426 343 252 163
SacII CC_GC'GG
Cuts at: 0 1151 2667
Size: 1151 1516
SanDI GG'GwC_CC
Cuts at: 0 182 1476 2006 2239 2667
Size: 182 1294 530 233 428
Fragments arranged by size:
1294 530 428 233 182
SapI GCTCTTCn'nnn_
Cuts at: 0 1049 1776 2667
Size: 1049 727 891
Fragments arranged by size:
1049 891 727
SbfI CC_TGCA'GG
Cuts at: 0 830 2667
Size: 830 1837
SexAI A'CCwGG_T
Cuts at: 0 2654 2667
Size: 2654 13
SfcI C'TryA_G
Cuts at: 0 826 911 917 1110 1512 2161 2257 2667
Size: 826 85 6 193 402 649 96 410
Fragments arranged by size:
826 649 410 402 193 96 85 6
SfoI GGC'GCC
Cuts at: 0 1520 2667
Size: 1520 1147
SmaI CCC'GGG
Cuts at: 0 1099 1837 2237 2667
Size: 1099 738 400 430
Fragments arranged by size:
1099 738 430 400
SmlI C'TyrA_G
Cuts at: 0 319 1946 2667
Size: 319 1627 721
Fragments arranged by size:
1627 721 319
SrfI GCCC'GGGC
Cuts at: 0 1099 2667
Size: 1099 1568
StuI AGG'CCT
Cuts at: 0 862 1612 2667
Size: 862 750 1055
Fragments arranged by size:
1055 862 750
StyI C'CwwG_G
Cuts at: 0 89 188 359 605 857 1613 1790 2667
Size: 89 99 171 246 252 756 177 877
Fragments arranged by size:
877 756 252 246 177 171 99 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1226 1695 2167 2311 2547 2667
Size: 1226 469 472 144 236 120
Fragments arranged by size:
1226 472 469 236 144 120
TatI w'GTAC_w
Cuts at: 0 2040 2667
Size: 2040 627
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1816 1848 2006 2038 2667
Size: 1816 32 158 32 629
Fragments arranged by size:
1816 629 158 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 93 1450 1665 1788 2278 2427 2667
Size: 93 1357 215 123 490 149 240
Fragments arranged by size:
1357 490 240 215 149 123 93
XhoI C'TCGA_G
Cuts at: 0 1946 2667
Size: 1946 721
XmaI C'CCGG_G
Cuts at: 0 1097 1835 2235 2667
Size: 1097 738 400 432
Fragments arranged by size:
1097 738 432 400
Enzymes that do cut:
AarI AclI AcuI AflIII AhdI AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI
BbvCI BciVI BglI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PasI PciI PflMI PfoI PmlI PpuMI PshAI PspOMI
PspXI PstI PvuII SacII SanDI SapI SbfI SexAI
SfcI SfoI SmaI SmlI SrfI StuI StyI TaqII
TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AgeI AloI AscI
AseI AsiSI AvrII BaeI BamHI BcgI BclI BfrBI
BglII BlpI BmgBI BmtI BsaBI BsaWI BsiWI BsmI
BspEI BspHI BssHII BssSI BstBI BstZ17I ClaI CspCI
DraI EcoICRI EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI NheI NotI NruI
NsiI PacI PmeI PpiI PsiI PsrI PvuI RsrII
SacI SalI ScaI SfiI SgfI SgrAI SnaBI SpeI
SphI SspI SwaI Tth111I XbaI XmnI ZraI