(Circular) (Six-base) MAPSORT of: pFN21ASDA0283.seq Check: 5067 from: 1 to: 9052 pFN21ASDA0283 9052 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4231 4231 Size: 9052 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4503 6862 278 Size: 53 83 186 936 2967 2359 2468 Fragments arranged by size: 2967 2468 2359 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2388 3042 3210 5043 6312 6395 1937 Size: 451 654 168 1833 1269 83 4594 Fragments arranged by size: 4594 1833 1269 654 451 168 83 Acc65I G'GTAC_C Cuts at: 6373 6373 Size: 9052 AclI AA'CG_TT Cuts at: 1563 7179 7552 1563 Size: 5616 373 3063 Fragments arranged by size: 5616 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2327 3008 3905 7115 8499 8767 1632 Size: 695 681 897 3210 1384 268 1917 Fragments arranged by size: 3210 1917 1384 897 695 681 268 AfeI AGC'GCT Cuts at: 6074 6074 Size: 9052 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8855 AflIII A'CryG_T Cuts at: 1203 6237 7951 8928 1203 Size: 5034 1714 977 1327 Fragments arranged by size: 5034 1714 1327 977 AgeI A'CCGG_T Cuts at: 7883 7883 Size: 9052 AhdI GACnn_n'nnGTC Cuts at: 4905 7781 4905 Size: 2876 6176 AleI CACnn'nnGTG Cuts at: 1166 2744 5274 1166 Size: 1578 2530 4944 Fragments arranged by size: 4944 2530 1578 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4911 1184 Size: 3727 5325 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4772 4804 4772 Size: 32 9020 AlwNI CAG_nnn'CTG Cuts at: 2142 2792 3482 6795 8367 2142 Size: 650 690 3313 1572 2827 Fragments arranged by size: 3313 2827 1572 690 650 ApaI G_GGCC'C Cuts at: 3513 3750 5259 5386 5916 3513 Size: 237 1509 127 530 6649 Fragments arranged by size: 6649 1509 530 237 127 ApaLI G'TGCA_C Cuts at: 5429 7109 8265 5429 Size: 1680 1156 6216 Fragments arranged by size: 6216 1680 1156 ApoI r'AATT_y Cuts at: 1493 3777 4213 5555 6305 6361 6587 6739 8848 Size: 2284 436 1342 750 56 226 152 2109 Cuts at: 8848 1493 Size: 1697 Fragments arranged by size: 2284 2109 1697 1342 750 436 226 152 56 AseI AT'TA_AT Cuts at: 160 1032 7606 160 Size: 872 6574 1606 Fragments arranged by size: 6574 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9052 AvaI C'yCGr_G Cuts at: 1870 1943 2091 3422 3628 3870 4605 4803 5594 Size: 73 148 1331 206 242 735 198 791 Cuts at: 5594 6377 1870 Size: 783 4545 Fragments arranged by size: 4545 1331 791 783 735 242 206 198 148 73 BamHI G'GATC_C Cuts at: 6382 6749 6382 Size: 367 8685 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2087 3964 4199 6373 7828 Size: 325 217 627 300 1877 235 2174 1455 Cuts at: 7828 618 Size: 1842 Fragments arranged by size: 2174 1877 1842 1455 627 325 300 235 217 BanII G_rGCy'C Cuts at: 1391 2098 2475 3223 3249 3513 3750 3880 4405 Size: 707 377 748 26 264 237 130 525 Cuts at: 4405 5082 5148 5259 5379 5386 5616 5916 6371 Size: 677 66 111 120 7 230 300 455 Cuts at: 6371 1391 Size: 4072 Fragments arranged by size: 4072 748 707 677 525 455 377 300 264 237 230 130 120 111 66 26 7 BbeI G_GCGC'C Cuts at: 2091 2091 Size: 9052 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3474 5900 928 Size: 971 1575 2426 4080 Fragments arranged by size: 4080 2426 1575 971 BbvCI CC'TCA_GC Cuts at: 2048 2048 Size: 9052 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7243 7277 1903 Size: 34 5306 34 3678 Fragments arranged by size: 5306 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4865 6944 8154 4865 Size: 2079 1210 5763 Fragments arranged by size: 5763 2079 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 9052 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5211 5460 7663 Size: 107 122 71 631 605 3539 249 2203 Cuts at: 7663 136 Size: 1525 Fragments arranged by size: 3539 2203 1525 631 605 249 122 107 71 BglII A'GATC_T Cuts at: 3525 9047 3525 Size: 5522 3530 BlpI GC'TnA_GC Cuts at: 4454 6469 4454 Size: 2015 7037 Bme1580I G_kGCm'C Cuts at: 1790 2232 3513 3750 3969 5259 5386 5433 5916 Size: 442 1281 237 219 1290 127 47 483 Cuts at: 5916 7113 8269 1790 Size: 1197 1156 2573 Fragments arranged by size: 2573 1290 1281 1197 1156 483 442 237 219 127 47 BmgBI CAC'GTC Cuts at: 1318 5653 6238 1318 Size: 4335 585 4132 Fragments arranged by size: 4335 4132 585 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5860 6783 7741 Size: 416 53 83 399 24 4443 923 958 Cuts at: 7741 442 Size: 1753 Fragments arranged by size: 4443 1753 958 923 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9052 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3122 5914 5950 3122 Size: 2792 36 6224 Fragments arranged by size: 6224 2792 36 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2335 2687 3973 5521 5824 6011 6122 7712 Size: 1096 352 1286 1548 303 187 111 1590 Cuts at: 7712 1239 Size: 2579 Fragments arranged by size: 2579 1590 1548 1286 1096 352 303 187 111 Bpu10I CC'TnA_GC Cuts at: 2048 2048 Size: 9052 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4887 7177 8042 8340 4887 Size: 2290 865 298 5599 Fragments arranged by size: 5599 2290 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2685 3023 3548 4157 4382 4824 6281 Size: 617 1186 338 525 609 225 442 1457 Cuts at: 6281 7715 882 Size: 1434 2219 Fragments arranged by size: 2219 1457 1434 1186 617 609 525 442 338 225 BsaAI yAC'GTr Cuts at: 493 1204 2392 3021 6349 6843 493 Size: 711 1188 629 3328 494 2702 Fragments arranged by size: 3328 2702 1188 711 629 494 BsaBI GATnn'nnATC Cuts at: 3530 5637 6006 9046 3530 Size: 2107 369 3040 3536 Fragments arranged by size: 3536 3040 2107 369 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2088 4500 6859 7241 Size: 53 83 186 936 555 2412 2359 382 Cuts at: 7241 7876 275 Size: 635 1451 Fragments arranged by size: 2412 2359 1451 936 635 555 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3178 3657 7485 7883 8157 8304 8715 3178 Size: 479 3828 398 274 147 411 3515 Fragments arranged by size: 3828 3515 479 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2333 2363 3867 3897 5813 Size: 30 165 30 930 30 1504 30 1916 Cuts at: 5813 5843 1178 Size: 30 4387 Fragments arranged by size: 4387 1916 1504 930 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3095 3343 3350 3458 3533 3926 4092 4274 Size: 1909 248 7 108 75 393 166 182 Cuts at: 4274 5426 5821 1186 Size: 1152 395 4417 Fragments arranged by size: 4417 1909 1152 395 393 248 182 166 108 75 7 BseYI C'CCAG_C Cuts at: 2457 2796 4050 4539 5252 5279 5285 5769 8255 Size: 339 1254 489 713 27 6 484 2486 Cuts at: 8255 8751 2457 Size: 496 2758 Fragments arranged by size: 2758 2486 1254 713 496 489 484 339 27 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3192 3314 3476 1163 Size: 573 1456 122 162 6739 Fragments arranged by size: 6739 1456 573 162 122 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6502 7263 8291 1540 Size: 275 186 4501 761 1028 2301 Fragments arranged by size: 4501 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2133 2475 3105 3880 5148 5379 5433 7113 7198 Size: 342 630 775 1268 231 54 1680 85 Cuts at: 7198 8269 2133 Size: 1071 2916 Fragments arranged by size: 2916 1680 1268 1071 775 630 342 231 85 54 BsmI GAATG_Cn' Cuts at: 2650 3988 6563 6656 2650 Size: 1338 2575 93 5046 Fragments arranged by size: 5046 2575 1338 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2530 5938 2530 Size: 3408 5644 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2098 2133 2232 2475 3105 3223 3249 Size: 399 308 35 99 243 630 118 26 Cuts at: 3249 3513 3750 3880 3969 4405 5082 5148 5259 Size: 264 237 130 89 436 677 66 111 Cuts at: 5259 5379 5386 5433 5616 5916 6371 7113 7198 Size: 120 7 47 183 300 455 742 85 Cuts at: 7198 8269 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 677 630 455 436 399 308 300 264 243 237 183 130 120 118 111 99 89 85 66 47 35 26 7 BspEI T'CCGG_A Cuts at: 3657 8715 3657 Size: 5058 3994 BspHI T'CATG_A Cuts at: 2244 3387 6941 8671 2244 Size: 1143 3554 1730 2625 Fragments arranged by size: 3554 2625 1730 1143 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4047 4231 6407 844 Size: 3203 184 2176 3489 Fragments arranged by size: 3489 3203 2176 184 BsrBI CCG'CTC Cuts at: 1394 1823 2832 3863 6939 1394 Size: 429 1009 1031 3076 3507 Fragments arranged by size: 3507 3076 1031 1009 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2661 2714 2895 4474 4893 5304 7547 Size: 1183 1413 53 181 1579 419 411 2243 Cuts at: 7547 7721 65 Size: 174 1396 Fragments arranged by size: 2243 1579 1413 1396 1183 419 411 181 174 53 BsrFI r'CCGG_y Cuts at: 1700 1810 7696 7883 1700 Size: 110 5886 187 2869 Fragments arranged by size: 5886 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9052 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9052 BssSI C'ACGA_G Cuts at: 7112 8124 7112 Size: 1012 8040 BstAPI GCAn_nnn'nTGC Cuts at: 794 2792 794 Size: 1998 7054 BstBI TT'CG_AA Cuts at: 2007 7856 2007 Size: 5849 3203 BstEII G'GTnAC_C Cuts at: 1184 2737 3262 5764 1184 Size: 1553 525 2502 4472 Fragments arranged by size: 4472 2502 1553 525 BstXI CCAn_nnnn'nTGG Cuts at: 4633 5791 5923 6035 6283 4633 Size: 1158 132 112 248 7402 Fragments arranged by size: 7402 1158 248 132 112 BstYI r'GATC_y Cuts at: 1972 2237 3093 3175 3525 5136 6382 6749 7134 Size: 265 856 82 350 1611 1246 367 385 Cuts at: 7134 7151 8603 8689 8701 9047 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1611 1452 1246 856 385 367 350 346 265 86 82 17 12 BstZ17I GTA'TAC Cuts at: 6313 6313 Size: 9052 Bsu36I CC'TnA_GG Cuts at: 2060 2060 Size: 9052 BtgI C'CryG_G Cuts at: 513 1065 2022 2556 4525 5179 5790 513 Size: 552 957 534 1969 654 611 3775 Fragments arranged by size: 3775 1969 957 654 611 552 534 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2536 487 Size: 683 56 1310 7003 Fragments arranged by size: 7003 1310 683 56 BtsI GCAGTG_nn' Cuts at: 2078 2232 2323 2790 3245 5269 5281 5466 5859 Size: 154 91 467 455 2024 12 185 393 Cuts at: 5859 6151 6205 6257 6568 7362 7382 2078 Size: 292 54 52 311 794 20 3748 Fragments arranged by size: 3748 2024 794 467 455 393 311 292 185 154 91 54 52 20 12 ClaI AT'CG_AT Cuts at: 1554 4771 1554 Size: 3217 5835 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2878 2913 3748 3783 4971 5006 529 Size: 35 2314 35 835 35 1188 35 4575 Fragments arranged by size: 4575 2314 1188 835 35 35 35 35 DraI TTT'AAA Cuts at: 6356 6356 Size: 9052 DraIII CAC_nnn'GTG Cuts at: 5585 6250 5585 Size: 665 8387 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5039 5057 8059 809 Size: 507 3723 18 3002 1802 Fragments arranged by size: 3723 3002 1802 507 18 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2275 2492 4371 Size: 54 1425 50 275 18 445 217 1879 Cuts at: 4371 5024 5331 5993 6499 7388 9017 8 Size: 653 307 662 506 889 1629 43 Fragments arranged by size: 1879 1629 1425 889 662 653 506 445 307 275 217 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6499 1537 Size: 275 4687 4090 Fragments arranged by size: 4687 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4489 4661 5159 6982 1353 Size: 3136 172 498 1823 3423 Fragments arranged by size: 3423 3136 1823 498 172 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4951 5138 5210 5305 7639 8013 Size: 1064 138 3362 187 72 95 2334 374 Cuts at: 8013 8159 387 Size: 146 1280 Fragments arranged by size: 3362 2334 1280 1064 374 187 146 138 95 72 EcoICRI GAG'CTC Cuts at: 2473 3878 5146 5377 2473 Size: 1405 1268 231 6148 Fragments arranged by size: 6148 1405 1268 231 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2327 2335 2687 3008 3905 3973 5521 Size: 393 695 8 352 321 897 68 1548 Cuts at: 5521 5824 6011 6122 7115 7712 8499 8767 1239 Size: 303 187 111 993 597 787 268 1524 Fragments arranged by size: 1548 1524 993 897 787 695 597 393 352 321 303 268 187 111 68 8 EcoO109I rG'GnC_Cy Cuts at: 2376 2572 3143 3509 3510 3747 4418 2376 Size: 196 571 366 1 237 671 7010 Fragments arranged by size: 7010 671 571 366 237 196 1 EcoRI G'AATT_C Cuts at: 4213 6361 6739 4213 Size: 2148 378 6526 Fragments arranged by size: 6526 2148 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4359 4359 Size: 9052 FspI TGC'GCA Cuts at: 7558 7558 Size: 9052 HaeII r_GCGC'y Cuts at: 1678 2091 2106 2764 4461 6076 8199 1678 Size: 413 15 658 1697 1615 2123 2531 Fragments arranged by size: 2531 2123 1697 1615 658 413 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2828 2860 3867 3899 4546 Size: 32 135 32 1256 32 1007 32 647 Cuts at: 4546 4578 4823 4855 4891 4923 5687 5719 7693 Size: 32 245 32 36 32 764 32 1974 Cuts at: 7693 7725 7767 7799 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1974 1256 1007 764 647 245 135 42 36 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2752 3043 4504 5395 6396 6642 Size: 988 281 814 291 1461 891 1001 246 Cuts at: 6642 669 Size: 3079 Fragments arranged by size: 3079 1461 1001 988 891 814 291 281 246 HindIII A'AGCT_T Cuts at: 748 2693 748 Size: 1945 7107 HpaI GTT'AAC Cuts at: 6642 6642 Size: 9052 KasI G'GCGC_C Cuts at: 2087 2087 Size: 9052 KpnI G_GTAC'C Cuts at: 6377 6377 Size: 9052 MfeI C'AATT_G Cuts at: 6651 6651 Size: 9052 MluI A'CGCG_T Cuts at: 8928 8928 Size: 9052 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2880 4642 6118 6300 8166 8350 1305 Size: 1575 1762 1476 182 1866 184 2007 Fragments arranged by size: 2007 1866 1762 1575 1476 184 182 MscI TGG'CCA Cuts at: 10 64 1489 1832 2277 2494 4373 5026 10 Size: 54 1425 343 445 217 1879 653 4036 Fragments arranged by size: 4036 1879 1425 653 445 343 217 54 MslI CAynn'nnrTG Cuts at: 518 1166 2249 2555 2656 2744 2935 3845 3848 Size: 648 1083 306 101 88 191 910 3 Cuts at: 3848 4631 4661 4973 5274 5477 5921 6236 7010 Size: 783 30 312 301 203 444 315 774 Cuts at: 7010 7369 7528 518 Size: 359 159 2042 Fragments arranged by size: 2042 1083 910 783 774 648 444 359 315 312 306 301 203 191 159 101 88 30 3 MspA1I CmG'CkG Cuts at: 1606 2071 2800 2820 3229 3270 3898 3941 4527 Size: 465 729 20 409 41 628 43 586 Cuts at: 4527 5169 5670 6468 7145 8293 8538 1606 Size: 642 501 798 677 1148 245 2120 Fragments arranged by size: 2120 1148 798 729 677 642 628 586 501 465 409 245 43 41 20 NarI GG'CG_CC Cuts at: 2088 2088 Size: 9052 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 7543 Fragments arranged by size: 7543 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9052 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9052 NotI GC'GGCC_GC Cuts at: 6499 6499 Size: 9052 NspI r_CATG'y Cuts at: 1132 1229 6410 7955 1132 Size: 97 5181 1545 2229 Fragments arranged by size: 5181 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2372 3695 1348 Size: 1024 1323 6705 Fragments arranged by size: 6705 1323 1024 PciI A'CATG_T Cuts at: 7951 7951 Size: 9052 PflMI CCAn_nnn'nTGG Cuts at: 1108 2623 6002 1108 Size: 1515 3379 4158 Fragments arranged by size: 4158 3379 1515 PfoI T'CCnGG_A Cuts at: 3475 5139 5442 3475 Size: 1664 303 7085 Fragments arranged by size: 7085 1664 303 PmeI GTTT'AAAC Cuts at: 6356 6356 Size: 9052 PmlI CAC'GTG Cuts at: 3021 3021 Size: 9052 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7121 7153 8634 8666 7121 Size: 32 1481 32 7507 Fragments arranged by size: 7507 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2572 2572 Size: 9052 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9052 PsiI TTA'TAA Cuts at: 6622 6622 Size: 9052 PspOMI G'GGCC_C Cuts at: 3509 3746 5255 5382 5912 3509 Size: 237 1509 127 530 6649 Fragments arranged by size: 6649 1509 530 237 127 PstI C_TGCA'G Cuts at: 830 2070 3240 4650 5276 5606 6200 6404 830 Size: 1240 1170 1410 626 330 594 204 3478 Fragments arranged by size: 3478 1410 1240 1170 626 594 330 204 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9052 PvuII CAG'CTG Cuts at: 2071 3229 3898 3941 5169 2071 Size: 1158 669 43 1228 5954 Fragments arranged by size: 5954 1228 1158 669 43 SacI G_AGCT'C Cuts at: 2475 3880 5148 5379 2475 Size: 1405 1268 231 6148 Fragments arranged by size: 6148 1405 1268 231 SacII CC_GC'GG Cuts at: 4528 4528 Size: 9052 SalI G'TCGA_C Cuts at: 1936 3041 6394 1936 Size: 1105 3353 4594 Fragments arranged by size: 4594 3353 1105 SbfI CC_TGCA'GG Cuts at: 6404 6404 Size: 9052 ScaI AGT'ACT Cuts at: 7300 9012 7300 Size: 1712 7340 SexAI A'CCwGG_T Cuts at: 3115 4986 5397 3115 Size: 1871 411 6770 Fragments arranged by size: 6770 1871 411 SfcI C'TryA_G Cuts at: 826 1045 2066 2134 3236 4123 4646 5272 5602 Size: 219 1021 68 1102 887 523 626 330 Cuts at: 5602 6196 6400 7535 8216 8407 826 Size: 594 204 1135 681 191 1471 Fragments arranged by size: 1471 1135 1102 1021 887 681 626 594 523 330 219 204 191 68 SfoI GGC'GCC Cuts at: 2089 2089 Size: 9052 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9052 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9052 SmaI CCC'GGG Cuts at: 1872 2093 3872 4607 4805 6379 1872 Size: 221 1779 735 198 1574 4545 Fragments arranged by size: 4545 1779 1574 735 221 198 SmlI C'TyrA_G Cuts at: 820 1017 1943 3628 4902 7156 8057 8319 820 Size: 197 926 1685 1274 2254 901 262 1553 Fragments arranged by size: 2254 1685 1553 1274 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6349 6843 493 Size: 5856 494 2702 Fragments arranged by size: 5856 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 9052 SphI G_CATG'C Cuts at: 1132 6410 1132 Size: 5278 3774 SrfI GCCC'GGGC Cuts at: 2093 2093 Size: 9052 SspI AAT'ATT Cuts at: 5 52 5970 6332 6976 5 Size: 47 5918 362 644 2081 Fragments arranged by size: 5918 2081 644 362 47 StuI AGG'CCT Cuts at: 1517 3375 4706 1517 Size: 1858 1331 5863 Fragments arranged by size: 5863 1858 1331 StyI C'CwwG_G Cuts at: 513 1065 2022 2413 3502 4743 6491 513 Size: 552 957 391 1089 1241 1748 3074 Fragments arranged by size: 3074 1748 1241 1089 957 552 391 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2336 2449 4055 4064 4223 5783 7079 Size: 1255 756 113 1606 9 159 1560 1296 Cuts at: 7079 7096 7249 7434 7879 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1606 1560 1498 1296 1255 756 445 185 159 153 113 17 9 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6030 7298 9010 Size: 274 80 33 51 1443 4053 1268 1712 Cuts at: 9010 96 Size: 138 Fragments arranged by size: 4053 1712 1443 1268 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3154 3186 6129 6161 9012 9044 3154 Size: 32 2943 32 2851 32 3162 Fragments arranged by size: 3162 2943 2851 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2564 1316 Size: 1248 7804 XbaI T'CTAG_A Cuts at: 3490 6388 3490 Size: 2898 6154 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2711 2779 4972 5206 5276 5326 6158 2711 Size: 68 2193 234 70 50 832 5605 Fragments arranged by size: 5605 2193 832 234 70 68 50 XhoI C'TCGA_G Cuts at: 1943 3628 1943 Size: 1685 7367 XmaI C'CCGG_G Cuts at: 1870 2091 3870 4605 4803 6377 1870 Size: 221 1779 735 198 1574 4545 Fragments arranged by size: 4545 1779 1574 735 221 198 XmnI GAAnn'nnTTC Cuts at: 3471 3647 7181 3471 Size: 176 3534 5342 Fragments arranged by size: 5342 3534 176 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4501 6860 276 Size: 53 83 186 936 2967 2359 2468 Fragments arranged by size: 2967 2468 2359 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SanDI SapI SfiI SwaI