Restriction map for
pFN21ASDA0282
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AocI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AvrII BalI BamHI
BbrPI BbvCI BcgI BclI BfrI
BlnI BsaAI BsaBI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CpoI DraI DraIII
EagI EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HpaI KpnI MamI MluI MluNI
MroI MscI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI RcaI RsrII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AhdI 1086 GACCACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1369 AGCGCT AGC^GCT
ApaI 604 GGGCCC GGGCC^C
AspEI 1086 GACCACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 893 TGCGCA TGC^GCA
BbeI 519 GGCGCC GGCGC^C
BciVI 1420 GGATAC GTATCC(6/5)
BglI 597 GCCCCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1055 AGATCT A^GATCT
BlpI 660 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 660 GCTGAGC GC^T[ACGT]AGC
BsaWI 1868 ACCGGA [AT]^CCGG[AT]
BspDI 970 ATCGAT AT^CGAT
BssSI 694 CACGAG CACGAG(-5/-1)
BstAPI 713 GCATGTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 660 GCTGAGC GC^T[ACGT]AGC
ClaI 970 ATCGAT AT^CGAT
DrdI 120 GACCCCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 326 CGGCCA [CT]^GGCC[GA]
Eam1105I 1086 GACCACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1369 AGCGCT AGC^GCT
EcoNI 1182 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 893 TGCGCA TGC^GCA
HincII 1012 GTCAAC GT[CT]^[GA]AC
HindII 1012 GTCAAC GT[CT]^[GA]AC
KasI 519 GGCGCC G^GCGCC
MfeI 1937 CAATTG C^AATTG
MseI 1121 TTAA T^TAA
MunI 1937 CAATTG C^AATTG
NarI 519 GGCGCC GG^CGCC
NspI 713 GCATGT [GA]CATG^[CT]
PpuMI 1943 GGGTCCT [GA]G^G[AT]CC[CT]
SacI 1744 GAGCTC GAGCT^C
ScaI 1103 AGTACT AGT^ACT
SfoI 519 GGCGCC GGC^GCC
SmaI 599 CCCGGG CCC^GGG
StuI 1541 AGGCCT AGG^CCT
Tru9I 1121 TTAA T^TAA
XmaCI 599 CCCGGG C^CCGGG
XmaI 599 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Alw44I 1177 GTGCAC G^TGCAC
1491 GTGCAC
ApaLI 1177 GTGCAC G^TGCAC
1491 GTGCAC
AvaI 599 CCCGGG C^[CT]CG[GA]G
1532 CTCGGG
BsmFI 282 GTCCC GGGAC(10/14)
1318 GTCCC
BsoBI 599 CCCGGG C^[CT]CG[GA]G
1532 CTCGGG
BspMI 379 ACCTGC ACCTGC(4/8)
682 ACCTGC
BsrDI 726 GCAATG GCAATG(2/0)
1849 GCAATG
BsrFI 324 ACCGGC [GA]^CCGG[CT]
342 ACCGGC
BstYI 911 AGATCC [GA]^GATC[CT]
1055 AGATCT
Cfr10I 324 ACCGGC [GA]^CCGG[CT]
342 ACCGGC
EarI 13 GAAGAG CTCTTC(1/4)
1172 GAAGAG
HindIII 1304 AAGCTT A^AGCTT
1841 AAGCTT
Ksp632I 13 GAAGAG CTCTTC(1/4)
1172 GAAGAG
MflI 911 AGATCC [GA]^GATC[CT]
1055 AGATCT
PshAI 1240 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1578 GACCCTGGTC
SfcI 1336 CTACAG C^T[GA][CT]AG
1677 CTGTAG
SmlI 496 CTCAAG C^T[CT][GA]AG
1242 CTTGAG
TfiI 133 GAATC G^A[AT]TC
441 GAATC
XhoII 911 AGATCC [GA]^GATC[CT]
1055 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KspI MaeI MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI