Restriction map for
pFN21ASDA0282
(Circular) (Six-base) MAPSORT of: pFN21ASDA0282.seq Check: 4334 from: 1 to: 6721
pFN21ASDA0282 6721 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2411 2714 2411
Size: 303 6418
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4531 278
Size: 53 83 186 936 2995 2468
Fragments arranged by size:
2995 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4064 1937
Size: 2127 4594
Acc65I G'GTAC_C
Cuts at: 4042 4042
Size: 6721
AclI AA'CG_TT
Cuts at: 1563 4848 5221 1563
Size: 3285 373 3063
Fragments arranged by size:
3285 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3080 4784 6168 6436 1632
Size: 1448 1704 1384 268 1917
Fragments arranged by size:
1917 1704 1448 1384 268
AfeI AGC'GCT
Cuts at: 3394 3394
Size: 6721
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6524
AflIII A'CryG_T
Cuts at: 1203 5620 6597 1203
Size: 4417 977 1327
Fragments arranged by size:
4417 1327 977
AgeI A'CCGG_T
Cuts at: 5552 5552
Size: 6721
AhdI GACnn_n'nnGTC
Cuts at: 3114 5450 3114
Size: 2336 4385
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6721
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6721
AlwNI CAG_nnn'CTG
Cuts at: 2153 2863 3025 4464 6036 2153
Size: 710 162 1439 1572 2838
Fragments arranged by size:
2838 1572 1439 710 162
ApaI G_GGCC'C
Cuts at: 2631 2631
Size: 6721
ApaLI G'TGCA_C
Cuts at: 3200 3514 4778 5934 3200
Size: 314 1264 1156 3987
Fragments arranged by size:
3987 1264 1156 314
ApoI r'AATT_y
Cuts at: 1493 4030 4256 4408 6517 1493
Size: 2537 226 152 2109 1697
Fragments arranged by size:
2537 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5275 160
Size: 872 4243 1606
Fragments arranged by size:
4243 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6721
AvaI C'yCGr_G
Cuts at: 1870 1943 2622 3555 4046 1870
Size: 73 679 933 491 4545
Fragments arranged by size:
4545 933 679 491 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2380 2413 2380
Size: 33 6688
BamHI G'GATC_C
Cuts at: 4051 4418 4051
Size: 367 6354
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2380 2542 2650 3430 4042
Size: 325 217 627 593 162 108 780 612
Cuts at: 4042 5497 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 780 627 612 593 325 217
162 108
BanII G_rGCy'C
Cuts at: 1391 2082 2186 2448 2631 2690 3771 4040 1391
Size: 691 104 262 183 59 1081 269 4072
Fragments arranged by size:
4072 1081 691 269 262 183 104 59
BbeI G_GCGC'C
Cuts at: 2546 2546
Size: 6721
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2575 3521 3740 928
Size: 971 676 946 219 3909
Fragments arranged by size:
3909 971 946 676 219
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4912 4946 1903
Size: 34 2975 34 3678
Fragments arranged by size:
3678 2975 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3437 4613 5823 3437
Size: 1176 1210 4335
Fragments arranged by size:
4335 1210 1176
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6721
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2626 5332 136
Size: 107 122 71 631 605 954 2706 1525
Fragments arranged by size:
2706 1525 954 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3078 6716 3078
Size: 3638 3083
BlpI GC'TnA_GC
Cuts at: 2684 4138 2684
Size: 1454 5267
Bme1580I G_kGCm'C
Cuts at: 1790 2385 2631 2655 3204 3435 3518 4782 5938
Size: 595 246 24 549 231 83 1264 1156
Cuts at: 5938 1790
Size: 2573
Fragments arranged by size:
2573 1264 1156 595 549 246 231 83
24
BmgBI CAC'GTC
Cuts at: 1318 3829 1318
Size: 2511 4210
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2404 3772 4452
Size: 416 53 83 399 24 987 1368 680
Cuts at: 4452 5410 442
Size: 958 1753
Fragments arranged by size:
1753 1368 987 958 680 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6721
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2744 2782 3321 3386 3473 5381 1239
Size: 1505 38 539 65 87 1908 2579
Fragments arranged by size:
2579 1908 1505 539 87 65 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2504 3286 4846 5711 6009 2504
Size: 782 1560 865 298 3216
Fragments arranged by size:
3216 1560 865 782 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2197 2704 3122 5384 882
Size: 617 698 507 418 2262 2219
Fragments arranged by size:
2262 2219 698 617 507 418
BsaAI yAC'GTr
Cuts at: 493 1204 4018 4512 493
Size: 711 2814 494 2702
Fragments arranged by size:
2814 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6715 6715
Size: 6721
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2316 2543 3183 4528
Size: 53 83 186 936 783 227 640 1345
Cuts at: 4528 4910 5545 275
Size: 382 635 1451
Fragments arranged by size:
1451 1345 936 783 640 635 382 227
186 83 53
BsaWI w'CCGG_w
Cuts at: 3891 5154 5552 5826 5973 6384 3891
Size: 1263 398 274 147 411 4228
Fragments arranged by size:
4228 1263 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3223 3253 3497 3527 1178
Size: 30 165 30 1820 30 244 30 4372
Fragments arranged by size:
4372 1820 244 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2054 2798 3962 1186
Size: 856 12 744 1164 3945
Fragments arranged by size:
3945 1164 856 744 12
BseYI C'CCAG_C
Cuts at: 3018 3343 5924 6420 3018
Size: 325 2581 496 3319
Fragments arranged by size:
3319 2581 496 325
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2159 2692 3073 1163
Size: 573 423 533 381 4811
Fragments arranged by size:
4811 573 533 423 381
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4171 4932 5960 1540
Size: 275 186 2170 761 1028 2301
Fragments arranged by size:
2301 2170 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3054 3204 3518 3771 4782 4867 5938 3054
Size: 150 314 253 1011 85 1071 3837
Fragments arranged by size:
3837 1071 1011 314 253 150 85
BsmI GAATG_Cn'
Cuts at: 4232 4325 4232
Size: 93 6628
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2186 2385 2448 2631 2655 2690
Size: 399 292 104 199 63 183 24 35
Cuts at: 2690 3054 3204 3435 3518 3771 4040 4782 4867
Size: 364 150 231 83 253 269 742 85
Cuts at: 4867 5938 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 364 292 269 253
231 199 183 150 104 85 83 63
35 24
BspEI T'CCGG_A
Cuts at: 6384 6384
Size: 6721
BspHI T'CATG_A
Cuts at: 4610 6340 4610
Size: 1730 4991
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2411 2714 4076 844
Size: 1567 303 1362 3489
Fragments arranged by size:
3489 1567 1362 303
BsrBI CCG'CTC
Cuts at: 1394 1823 4608 1394
Size: 429 2785 3507
Fragments arranged by size:
3507 2785 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2756 3879 5216 5390 65
Size: 1183 1508 1123 1337 174 1396
Fragments arranged by size:
1508 1396 1337 1183 1123 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2347 2365 5365 5552 1700
Size: 110 537 18 3000 187 2869
Fragments arranged by size:
3000 2869 537 187 110 18
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6721
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6721
BssSI C'ACGA_G
Cuts at: 2717 4781 5793 2717
Size: 2064 1012 3645
Fragments arranged by size:
3645 2064 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2742 794
Size: 1948 4773
BstBI TT'CG_AA
Cuts at: 2007 5525 2007
Size: 3518 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6721
BstYI r'GATC_y
Cuts at: 1972 2934 3078 4051 4418 4803 4820 6272 6358
Size: 962 144 973 367 385 17 1452 86
Cuts at: 6358 6370 6716 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 973 962 385 367 346 144
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2178 2449 2511 3252 3426 513
Size: 552 957 156 271 62 741 174 3808
Fragments arranged by size:
3808 957 741 552 271 174 156 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2592 487
Size: 683 56 1366 4616
Fragments arranged by size:
4616 1366 683 56
BtsI GCAGTG_nn'
Cuts at: 3085 4237 5031 5051 3085
Size: 1152 794 20 4755
Fragments arranged by size:
4755 1152 794 20
ClaI AT'CG_AT
Cuts at: 1554 2994 1554
Size: 1440 5281
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3241 3276 3861 3896 529
Size: 35 2677 35 585 35 3354
Fragments arranged by size:
3354 2677 585 35 35 35
DraI TTT'AAA
Cuts at: 4025 4025
Size: 6721
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2149 5728 809
Size: 507 833 3579 1802
Fragments arranged by size:
3579 1802 833 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2349 4168 5057
Size: 54 1425 50 275 18 519 1819 889
Cuts at: 5057 6686 8
Size: 1629 43
Fragments arranged by size:
1819 1629 1425 889 519 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4168 1537
Size: 275 2356 4090
Fragments arranged by size:
4090 2356 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2031 3190 4651 1353
Size: 678 1159 1461 3423
Fragments arranged by size:
3423 1461 1159 678
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2258 5308 5682 5828 387
Size: 1064 138 669 3050 374 146 1280
Fragments arranged by size:
3050 1280 1064 669 374 146 138
EcoICRI GAG'CTC
Cuts at: 3769 3769
Size: 6721
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2744 2782 3080 3321 3386 3473 4784
Size: 393 1112 38 298 241 65 87 1311
Cuts at: 4784 5381 6168 6436 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1311 1112 787 597 393 298 268
241 87 65 38
EcoNI CCTnn'n_nnAGG
Cuts at: 3209 3209
Size: 6721
EcoO109I rG'GnC_Cy
Cuts at: 2627 2628 3967 2627
Size: 1 1339 5381
Fragments arranged by size:
5381 1339 1
EcoRI G'AATT_C
Cuts at: 4030 4408 4030
Size: 378 6343
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3757 3757
Size: 6721
FspI TGC'GCA
Cuts at: 2918 5227 2918
Size: 2309 4412
HaeII r_GCGC'y
Cuts at: 1678 2283 2546 2864 3396 5868 1678
Size: 605 263 318 532 2472 2531
Fragments arranged by size:
2531 2472 605 532 318 263
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2141 2173 3063 3065 3095
Size: 32 135 32 569 32 890 2 30
Cuts at: 3095 3097 3100 3132 3199 3231 3635 3667 5362
Size: 2 3 32 67 32 404 32 1695
Cuts at: 5362 5394 5436 5468 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1695 890 569 404 135 67 42
32 32 32 32 32 32 32 32
30 3 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3037 4065 4311 669
Size: 988 281 1099 1028 246 3079
Fragments arranged by size:
3079 1099 1028 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3327 3864 748
Size: 2579 537 3605
Fragments arranged by size:
3605 2579 537
HpaI GTT'AAC
Cuts at: 4311 4311
Size: 6721
KasI G'GCGC_C
Cuts at: 2542 2542
Size: 6721
KpnI G_GTAC'C
Cuts at: 4046 4046
Size: 6721
MfeI C'AATT_G
Cuts at: 3960 4320 3960
Size: 360 6361
MluI A'CGCG_T
Cuts at: 6597 6597
Size: 6721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2220 5835 6019 1305
Size: 915 3615 184 2007
Fragments arranged by size:
3615 2007 915 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4899
Fragments arranged by size:
4899 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2135 2561 3827 4679 5038 5197 518
Size: 648 969 426 1266 852 359 159 2042
Fragments arranged by size:
2042 1266 969 852 648 426 359 159
MspA1I CmG'CkG
Cuts at: 1606 2180 2250 2451 2513 2914 3022 3307 3364
Size: 574 70 201 62 401 108 285 57
Cuts at: 3364 4137 4814 5962 6207 1606
Size: 773 677 1148 245 2120
Fragments arranged by size:
2120 1148 773 677 574 401 285 245
201 108 70 62 57
NarI GG'CG_CC
Cuts at: 2543 2543
Size: 6721
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5212
Fragments arranged by size:
5212 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6721
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6721
NotI GC'GGCC_GC
Cuts at: 4168 4168
Size: 6721
NspI r_CATG'y
Cuts at: 1132 1229 2740 4079 5624 1132
Size: 97 1511 1339 1545 2229
Fragments arranged by size:
2229 1545 1511 1339 97
PasI CC'CwG_GG
Cuts at: 1348 2662 1348
Size: 1314 5407
PciI A'CATG_T
Cuts at: 5620 5620
Size: 6721
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2722 2779 3025 3307 1108
Size: 1614 57 246 282 4522
Fragments arranged by size:
4522 1614 282 246 57
PfoI T'CCnGG_A
Cuts at: 2430 2937 3404 3483 2430
Size: 507 467 79 5668
Fragments arranged by size:
5668 507 467 79
PmeI GTTT'AAAC
Cuts at: 4025 4025
Size: 6721
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4790 4822 6303 6335 4790
Size: 32 1481 32 5176
Fragments arranged by size:
5176 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3967 3967
Size: 6721
PshAI GACnn'nnGTC
Cuts at: 1613 3267 3605 1613
Size: 1654 338 4729
Fragments arranged by size:
4729 1654 338
PsiI TTA'TAA
Cuts at: 4291 4291
Size: 6721
PspOMI G'GGCC_C
Cuts at: 2627 2627
Size: 6721
PstI C_TGCA'G
Cuts at: 830 4073 830
Size: 3243 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6721
PvuII CAG'CTG
Cuts at: 2914 3022 3307 3364 2914
Size: 108 285 57 6271
Fragments arranged by size:
6271 285 108 57
SacI G_AGCT'C
Cuts at: 3771 3771
Size: 6721
SacII CC_GC'GG
Cuts at: 2181 2452 2514 2181
Size: 271 62 6388
Fragments arranged by size:
6388 271 62
SalI G'TCGA_C
Cuts at: 1936 4063 1936
Size: 2127 4594
SbfI CC_TGCA'GG
Cuts at: 4073 4073
Size: 6721
ScaI AGT'ACT
Cuts at: 3128 4969 6681 3128
Size: 1841 1712 3168
Fragments arranged by size:
3168 1841 1712
SfcI C'TryA_G
Cuts at: 826 1045 3359 3700 4069 5204 5885 6076 826
Size: 219 2314 341 369 1135 681 191 1471
Fragments arranged by size:
2314 1471 1135 681 369 341 219 191
SfoI GGC'GCC
Cuts at: 2544 2544
Size: 6721
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6721
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6721
SmaI CCC'GGG
Cuts at: 1872 2624 4048 1872
Size: 752 1424 4545
Fragments arranged by size:
4545 1424 752
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2519 3265 4825 5726 5988 820
Size: 197 926 576 746 1560 901 262 1553
Fragments arranged by size:
1560 1553 926 901 746 576 262 197
SnaBI TAC'GTA
Cuts at: 493 4018 4512 493
Size: 3525 494 2702
Fragments arranged by size:
3525 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6721
SphI G_CATG'C
Cuts at: 1132 4079 1132
Size: 2947 3774
SspI AAT'ATT
Cuts at: 5 52 4645 5
Size: 47 4593 2081
Fragments arranged by size:
4593 2081 47
StuI AGG'CCT
Cuts at: 1517 3566 1517
Size: 2049 4672
StyI C'CwwG_G
Cuts at: 513 1065 2022 2547 2895 3242 3747 4160 513
Size: 552 957 525 348 347 505 413 3074
Fragments arranged by size:
3074 957 552 525 505 413 348 347
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2369 4748 4765 4918 5103 5548 325
Size: 1255 789 2379 17 153 185 445 1498
Fragments arranged by size:
2379 1498 1255 789 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2739 3126 4967
Size: 274 80 33 51 1443 762 387 1841
Cuts at: 4967 6679 96
Size: 1712 138
Fragments arranged by size:
1841 1712 1443 762 387 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3223 3255 3497 3529 6681 6713 3223
Size: 32 242 32 3152 32 3231
Fragments arranged by size:
3231 3152 242 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6721
XbaI T'CTAG_A
Cuts at: 4057 4057
Size: 6721
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2644 2902 3118 3304 2029
Size: 615 258 216 186 5446
Fragments arranged by size:
5446 615 258 216 186
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6721
XmaI C'CCGG_G
Cuts at: 1870 2622 4046 1870
Size: 752 1424 4545
Fragments arranged by size:
4545 1424 752
XmnI GAAnn'nnTTC
Cuts at: 4850 4850
Size: 6721
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4529 276
Size: 53 83 186 936 2995 2468
Fragments arranged by size:
2995 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbvCI BfrBI BplI Bpu10I BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SanDI SapI SexAI SfiI SrfI SwaI