Restriction map for
pFN21ASDA0280
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspHI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiHKAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstYI BstZ17I Bsu36I CelII CfoI
ClaI CpoI DpnII DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
Ksp632I KspI MaeI MamI MboI
MfeI MflI MluI MroI MseI
MunI MvnI NaeI NarI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI Tru9I Tsp45I Tsp509I
Tth111I TthHB8I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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Acc65I 622 GGTACC G^GTACC
ApaI 689 GGGCCC GGGCC^C
Asp718I 622 GGTACC G^GTACC
AvaI 561 CCCGAG C^[CT]CG[GA]G
BbsI 216 GTCTTC GAAGAC(2/6)
BciVI 402 GTATCC GTATCC(6/5)
BcnI 260 CCGGG CC^[GC]GG
BpmI 146 CTCCAG CTGGAG(16/14)
BpuAI 216 GTCTTC GAAGAC(2/6)
BsaAI 715 TACGTG [CT]AC^GT[GA]
BsgI 22 GTGCAG GTGCAG(16/14)
BsiEI 506 CGGTCG CG[GA][CT]^CG
BsmAI 670 GTCTC GTCTC(1/5)
BsoBI 561 CCCGAG C^[CT]CG[GA]G
BspMI 619 GCAGGT ACCTGC(4/8)
BsrDI 610 GCAATG GCAATG(2/0)
BsrFI 465 GCCGGT [GA]^CCGG[CT]
BstEII 70 GGTTACC G^GT[ACGT]ACC
BstPI 70 GGTTACC G^GT[ACGT]ACC
Cac8I 595 GCAGGC GC[ACGT]^[ACGT]GC
Cfr10I 465 GCCGGT [GA]^CCGG[CT]
DdeI 50 CTAAG C^T[ACGT]AG
EcoO65I 70 GGTTACC G^GT[ACGT]ACC
FauI 313 GCGGG CCCGC(4/6)
HinfI 432 GAATC G^A[ACGT]TC
HphI 376 TCACC GGTGA(8/7)
KpnI 622 GGTACC GGTAC^C
MaeII 716 ACGT A^CGT
MboII 217 TCTTC GAAGA(8/7)
MspA1I 92 CAGCTG C[AC]G^C[GT]G
NciI 260 CCGGG CC^[GC]GG
PpuMI 329 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 92 CAGCTG CAG^CTG
SfaNI 286 GCATC GCATC(5/9)
StuI 197 AGGCCT AGG^CCT
TfiI 432 GAATC G^A[AT]TC
XcmI 581 CCATGGGCATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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BalI 202 TGGCCA TGG^CCA
578 TGGCCA
BanII 32 GGGCTC G[GA]GC[CT]^C
689 GGGCCC
BsmI 165 GCATTC GAATGC(1/-1)
524 GCATTC
BstAPI 286 GCATCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
547 GCACCTGTTGC
BstF5I 287 CATCC GGATG(2/0)
492 CATCC
BstXI 569 CCAACGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
581 CCATGGGCATGG
DraII 329 AGGTCCC [GA]G^G[ACGT]CC[CT]
688 AGGGCCC
EcoO109I 329 AGGTCCC [GA]G^G[ACGT]CC[CT]
688 AGGGCCC
FokI 287 CATCC GGATG(9/13)
492 CATCC
HapII 260 CCGG C^CGG
466 CCGG
HpaII 260 CCGG C^CGG
466 CCGG
MaeIII 71 GTTAC ^GT[ACGT]AC
347 GTAAC
MluNI 202 TGGCCA TGG^CCA
578 TGGCCA
MscI 202 TGGCCA TGG^CCA
578 TGGCCA
MspI 260 CCGG C^CGG
466 CCGG
NcoI 581 CCATGG C^CATGG
605 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BanI BbvI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstNI Cfr13I DsaI EaeI EcoRII
EcoT14I Fnu4HI HaeIII ItaI MnlI
MslI MvaI MwoI NlaIII NlaIV
RsaI Sau96I ScrFI StyI TseI
TspRI