Restriction map for
pFN21ASDA0280
(Linear) (Six-base) MAPSORT of: KIAA0280.seq Check: 8259 from: 1 to: 732
KIAA0280.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
Acc65I G'GTAC_C
Cuts at: 0 622 732
Size: 622 110
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 732
Size: 251 481
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 202 234 732
Size: 202 32 498
Fragments arranged by size:
498 202 32
AlwNI CAG_nnn'CTG
Cuts at: 0 94 162 435 550 732
Size: 94 68 273 115 182
Fragments arranged by size:
273 182 115 94 68
ApaI G_GGCC'C
Cuts at: 0 693 732
Size: 693 39
AvaI C'yCGr_G
Cuts at: 0 561 732
Size: 561 171
BanI G'GyrC_C
Cuts at: 0 235 598 622 732
Size: 235 363 24 110
Fragments arranged by size:
363 235 110 24
BanII G_rGCy'C
Cuts at: 0 36 693 732
Size: 36 657 39
Fragments arranged by size:
657 39 36
BbsI GAAGACnn'nnnn_
Cuts at: 0 209 732
Size: 209 523
BciVI GTATCCnnnnn_n'
Cuts at: 0 413 732
Size: 413 319
Bme1580I G_kGCm'C
Cuts at: 0 663 693 722 732
Size: 663 30 29 10
Fragments arranged by size:
663 30 29 10
BmrI ACTGGGnnnn_n'
Cuts at: 0 131 244 732
Size: 131 113 488
Fragments arranged by size:
488 131 113
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 732
Size: 131 601
BsaAI yAC'GTr
Cuts at: 0 717 732
Size: 717 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 655 685 732
Size: 655 30 47
Fragments arranged by size:
655 47 30
BseYI C'CCAG_C
Cuts at: 0 112 425 479 543 641 732
Size: 112 313 54 64 98 91
Fragments arranged by size:
313 112 98 91 64 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 732
Size: 43 689
BsiEI CG_ry'CG
Cuts at: 0 509 732
Size: 509 223
BsmI GAATG_Cn'
Cuts at: 0 165 524 732
Size: 165 359 208
Fragments arranged by size:
359 208 165
Bsp1286I G_dGCh'C
Cuts at: 0 36 663 693 722 732
Size: 36 627 30 29 10
Fragments arranged by size:
627 36 30 29 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 610 732
Size: 610 122
BsrDI GCAATG_nn'
Cuts at: 0 617 732
Size: 617 115
BsrFI r'CCGG_y
Cuts at: 0 465 732
Size: 465 267
BstAPI GCAn_nnn'nTGC
Cuts at: 0 292 553 732
Size: 292 261 179
Fragments arranged by size:
292 261 179
BstEII G'GTnAC_C
Cuts at: 0 70 732
Size: 70 662
BstXI CCAn_nnnn'nTGG
Cuts at: 0 576 588 732
Size: 576 12 144
Fragments arranged by size:
576 144 12
BtgI C'CryG_G
Cuts at: 0 503 581 605 732
Size: 503 78 24 127
Fragments arranged by size:
503 127 78 24
BtsI GCAGTG_nn'
Cuts at: 0 350 732
Size: 350 382
EaeI y'GGCC_r
Cuts at: 0 202 395 578 732
Size: 202 193 183 154
Fragments arranged by size:
202 193 183 154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 251 732
Size: 131 120 481
Fragments arranged by size:
481 131 120
EcoO109I rG'GnC_Cy
Cuts at: 0 330 689 732
Size: 330 359 43
Fragments arranged by size:
359 330 43
KpnI G_GTAC'C
Cuts at: 0 626 732
Size: 626 106
MscI TGG'CCA
Cuts at: 0 204 580 732
Size: 204 376 152
Fragments arranged by size:
376 204 152
MslI CAynn'nnrTG
Cuts at: 0 586 604 610 732
Size: 586 18 6 122
Fragments arranged by size:
586 122 18 6
MspA1I CmG'CkG
Cuts at: 0 94 732
Size: 94 638
NcoI C'CATG_G
Cuts at: 0 581 605 732
Size: 581 24 127
Fragments arranged by size:
581 127 24
PasI CC'CwG_GG
Cuts at: 0 444 732
Size: 444 288
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 142 174 732
Size: 142 32 558
Fragments arranged by size:
558 142 32
PpuMI rG'GwC_Cy
Cuts at: 0 330 732
Size: 330 402
PspOMI G'GGCC_C
Cuts at: 0 689 732
Size: 689 43
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 27 46 78 262 294 732
Size: 27 19 32 184 32 438
Fragments arranged by size:
438 184 32 32 27 19
PvuII CAG'CTG
Cuts at: 0 94 732
Size: 94 638
StuI AGG'CCT
Cuts at: 0 199 732
Size: 199 533
StyI C'CwwG_G
Cuts at: 0 581 605 693 732
Size: 581 24 88 39
Fragments arranged by size:
581 88 39 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 280 393 732
Size: 280 113 339
Fragments arranged by size:
339 280 113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 69 101 117 149 558 590 655 687
Size: 69 32 16 32 409 32 65 32
Cuts at: 687 732
Size: 45
Fragments arranged by size:
409 69 65 45 32 32 32 32
16
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 588 732
Size: 588 144
Enzymes that do cut:
Acc65I AcuI AloI AlwNI ApaI AvaI BanI BanII
BbsI BciVI Bme1580I BmrI BpmI BsaAI BsaXI BseYI
BsgI BsiEI BsmI Bsp1286I BspMI BsrDI BsrFI BstAPI
BstEII BstXI BtgI BtsI EaeI Eco57MI EcoO109I KpnI
MscI MslI MspA1I NcoI PasI PpiI PpuMI PspOMI
PsrI PvuII StuI StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BseRI BsiHKAI BsiWI BsmBI
BspEI BspHI BsrBI BsrGI BssHII BssSI BstBI BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PflMI
PfoI PmeI PmlI PshAI PsiI PspXI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I
XbaI XhoI XmaI XmnI ZraI