Restriction map for
pFN21ASDA0276
##################################
# NO CUT ENZYMES ( 176 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AfaI AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BamHI
BanI BanII BbeI BbrPI BcgI
BciVI BclI BcnI BlnI BlpI
BmyI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssKI BssSI Bst1107I
BstAPI BstBI BstEII BstNI BstPI
BstUI BstXI BstZ17I Bsu36I Cac8I
CelII Cfr10I ClaI CpoI DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRII EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MaeIII MamI MfeI
MluI MroI MspI MunI MvaI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
ScrFI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tsp45I
Tth111I Van91I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 239 GTAGAC GT^[AC][GT]AC
AcsI 379 AAATTT [GA]^AATT[CT]
AflII 546 CTTAAG C^TTAAG
AlwNI 341 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 379 AAATTT [GA]^AATT[CT]
Asp700I 491 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 621 GGACC G^G[AT]CC
BbvCI 795 CCTCAGC CCTCAGC(-5/-2)
BbvI 629 GCAGC GCAGC(8/12)
BfrI 546 CTTAAG C^TTAAG
BglI 362 GCCCTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 71 AGATCT A^GATCT
BpmI 212 CTGGAG CTGGAG(16/14)
BsaJI 187 CCAAGG C^C[ACGT][ACGT]GG
BsiYI 205 CCTGCCTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 205 CCTGCCTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 663 GGGAC GGGAC(10/14)
BspMI 204 ACCTGC ACCTGC(4/8)
BsrDI 758 GCAATG GCAATG(2/0)
BstYI 71 AGATCT [GA]^GATC[CT]
CfoI 627 GCGC GCG^C
DraI 577 TTTAAA TTT^AAA
DraII 360 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 360 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 187 CCAAGG C^C[AT][AT]GG
HhaI 627 GCGC GCG^C
HinP1I 627 GCGC G^CGC
MaeII 413 ACGT A^CGT
MflI 71 AGATCT [GA]^GATC[CT]
MslI 4 CACTCGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 362 GCCCTGAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 686 GGCCCC GG[ACGT]^[ACGT]CC
PvuII 31 CAGCTG CAG^CTG
SfcI 472 CTACAG C^T[GA][CT]AG
SmlI 546 CTTAAG C^T[CT][GA]AG
SspI 61 AATATT AAT^ATT
StyI 187 CCAAGG C^C[AT][AT]GG
TaqI 271 TCGA T^CGA
TseI 629 GCAGC G^C[AT]GC
TthHB8I 271 TCGA T^CGA
XcmI 650 CCAAGTGCATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 71 AGATCT [GA]^GATC[CT]
XmnI 491 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AseI 739 ATTAAT AT^TAAT
784 ATTAAT
AsnI 739 ATTAAT AT^TAAT
784 ATTAAT
BalI 114 TGGCCA TGG^CCA
823 TGGCCA
BstF5I 9 GGATG GGATG(2/0)
444 GGATG
EaeI 114 TGGCCA [CT]^GGCC[GA]
823 TGGCCA
FokI 9 GGATG GGATG(9/13)
444 GGATG
HinfI 268 GATTC G^A[ACGT]TC
565 GATTC
HphI 126 TCACC GGTGA(8/7)
705 TCACC
MluNI 114 TGGCCA TGG^CCA
823 TGGCCA
MnlI 209 CCTC CCTC(7/6)
795 CCTC
MscI 114 TGGCCA TGG^CCA
823 TGGCCA
MspA1I 17 CCGCTG C[AC]G^C[GT]G
31 CAGCTG
NspI 251 GCATGT [GA]CATG^[CT]
656 GCATGT
PshBI 739 ATTAAT AT^TAAT
784 ATTAAT
TfiI 268 GATTC G^A[AT]TC
565 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AluI BbsI BfaI BpuAI
BsrI Cfr13I DdeI DpnII Fnu4HI
HaeIII ItaI MaeI MboI MboII
MseI NdeII NlaIII Sau3AI Sau96I
SfaNI Tru9I Tsp509I TspRI