(Circular) (Six-base) MAPSORT of: pFN21ASDA0275.seq Check: 1216 from: 1 to: 6001 pFN21ASDA0275 6001 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3811 278 Size: 53 83 186 936 2275 2468 Fragments arranged by size: 2468 2275 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3344 1937 Size: 1407 4594 Acc65I G'GTAC_C Cuts at: 3322 3322 Size: 6001 AclI AA'CG_TT Cuts at: 1563 4128 4501 1563 Size: 2565 373 3063 Fragments arranged by size: 3063 2565 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4064 5448 5716 1632 Size: 2432 1384 268 1917 Fragments arranged by size: 2432 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5804 AflIII A'CryG_T Cuts at: 1203 4900 5877 1203 Size: 3697 977 1327 Fragments arranged by size: 3697 1327 977 AgeI A'CCGG_T Cuts at: 2621 4832 2621 Size: 2211 3790 AhdI GACnn_n'nnGTC Cuts at: 4730 4730 Size: 6001 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6001 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6001 AlwNI CAG_nnn'CTG Cuts at: 2632 3744 5316 2632 Size: 1112 1572 3317 Fragments arranged by size: 3317 1572 1112 ApaI G_GGCC'C Cuts at: 2558 2558 Size: 6001 ApaLI G'TGCA_C Cuts at: 4058 5214 4058 Size: 1156 4845 ApoI r'AATT_y Cuts at: 1493 3310 3536 3688 5797 1493 Size: 1817 226 152 2109 1697 Fragments arranged by size: 2109 1817 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4555 160 Size: 872 3523 1606 Fragments arranged by size: 3523 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6001 AvaI C'yCGr_G Cuts at: 1870 1943 2032 3025 3169 3326 1870 Size: 73 89 993 144 157 4545 Fragments arranged by size: 4545 993 157 144 89 73 BamHI G'GATC_C Cuts at: 3331 3698 3331 Size: 367 5634 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3322 4777 618 Size: 325 217 627 1535 1455 1842 Fragments arranged by size: 1842 1535 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2109 2558 3143 3320 1391 Size: 718 449 585 177 4072 Fragments arranged by size: 4072 718 585 449 177 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5030 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4192 4226 1903 Size: 34 2255 34 3678 Fragments arranged by size: 3678 2255 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2266 3893 5103 2266 Size: 1627 1210 3164 Fragments arranged by size: 3164 1627 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6001 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2723 4612 136 Size: 107 122 71 631 605 1051 1889 1525 Fragments arranged by size: 1889 1525 1051 631 605 122 107 71 BglII A'GATC_T Cuts at: 5996 5996 Size: 6001 BlpI GC'TnA_GC Cuts at: 2701 3418 2701 Size: 717 5284 Bme1580I G_kGCm'C Cuts at: 1790 2558 4062 5218 1790 Size: 768 1504 1156 2573 Fragments arranged by size: 2573 1504 1156 768 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6001 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3732 4690 442 Size: 416 53 83 399 24 2315 958 1753 Fragments arranged by size: 2315 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6001 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2393 2525 2962 2993 3131 4661 1239 Size: 1154 132 437 31 138 1530 2579 Fragments arranged by size: 2579 1530 1154 437 138 132 31 Bpu10I CC'TnA_GC Cuts at: 2522 2522 Size: 6001 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2093 4126 4991 5289 2093 Size: 2033 865 298 2805 Fragments arranged by size: 2805 2033 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2114 2917 4664 882 Size: 617 615 803 1747 2219 Fragments arranged by size: 2219 1747 803 617 615 BsaAI yAC'GTr Cuts at: 493 1204 3298 3792 493 Size: 711 2094 494 2702 Fragments arranged by size: 2702 2094 711 494 BsaBI GATnn'nnATC Cuts at: 5995 5995 Size: 6001 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2097 3808 4190 4825 Size: 53 83 186 936 564 1711 382 635 Cuts at: 4825 275 Size: 1451 Fragments arranged by size: 1711 1451 936 635 564 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2621 3051 4434 4832 5106 5253 5664 2621 Size: 430 1383 398 274 147 411 2958 Fragments arranged by size: 2958 1383 430 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2405 2435 1178 Size: 30 165 30 1002 30 4744 Fragments arranged by size: 4744 1002 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3221 3224 3251 3260 3263 3266 1186 Size: 2035 3 27 9 3 3 3921 Fragments arranged by size: 3921 2035 27 9 3 3 3 BseYI C'CCAG_C Cuts at: 2238 2448 3103 3195 5204 5700 2238 Size: 210 655 92 2009 496 2539 Fragments arranged by size: 2539 2009 655 496 210 92 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2326 1163 Size: 573 590 4838 Fragments arranged by size: 4838 590 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2397 3451 4212 5240 1540 Size: 275 186 396 1054 761 1028 2301 Fragments arranged by size: 2301 1054 1028 761 396 275 186 BsiHKAI G_wGCw'C Cuts at: 2379 4062 4147 5218 2379 Size: 1683 85 1071 3162 Fragments arranged by size: 3162 1683 1071 85 BsmI GAATG_Cn' Cuts at: 3512 3605 3512 Size: 93 5908 BsmBI CGTCTCn'nnnn_ Cuts at: 2202 3212 2202 Size: 1010 4991 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2109 2379 2558 3143 3320 4062 4147 Size: 399 319 270 179 585 177 742 85 Cuts at: 4147 5218 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 585 399 319 270 179 177 85 BspEI T'CCGG_A Cuts at: 5664 5664 Size: 6001 BspHI T'CATG_A Cuts at: 2677 3890 5620 2677 Size: 1213 1730 3058 Fragments arranged by size: 3058 1730 1213 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3356 844 Size: 2512 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3888 1394 Size: 429 2065 3507 Fragments arranged by size: 3507 2065 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2156 2325 4496 4670 65 Size: 1183 908 169 2171 174 1396 Fragments arranged by size: 2171 1396 1183 908 174 169 BsrFI r'CCGG_y Cuts at: 1700 1810 2621 2717 4645 4832 1700 Size: 110 811 96 1928 187 2869 Fragments arranged by size: 2869 1928 811 187 110 96 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6001 BssHII G'CGCG_C Cuts at: 1925 2026 1925 Size: 101 5900 BssSI C'ACGA_G Cuts at: 4061 5073 4061 Size: 1012 4989 BstAPI GCAn_nnn'nTGC Cuts at: 794 2436 794 Size: 1642 4359 BstBI TT'CG_AA Cuts at: 2007 4805 2007 Size: 2798 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6001 BstXI CCAn_nnnn'nTGG Cuts at: 2734 2845 2734 Size: 111 5890 BstYI r'GATC_y Cuts at: 1972 2985 3331 3698 4083 4100 5552 5638 5650 Size: 1013 346 367 385 17 1452 86 12 Cuts at: 5650 5996 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1013 385 367 346 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2073 2407 2571 513 Size: 552 1008 334 164 3943 Fragments arranged by size: 3943 1008 552 334 164 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2560 3165 487 Size: 683 56 1334 605 3323 Fragments arranged by size: 3323 1334 683 605 56 BtsI GCAGTG_nn' Cuts at: 2716 3068 3517 4311 4331 2716 Size: 352 449 794 20 4386 Fragments arranged by size: 4386 794 449 352 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6001 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5966 DraI TTT'AAA Cuts at: 3305 3305 Size: 6001 DraIII CAC_nnn'GTG Cuts at: 2320 2320 Size: 6001 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5008 809 Size: 507 3692 1802 Fragments arranged by size: 3692 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2070 2085 2476 Size: 54 1425 50 275 18 240 15 391 Cuts at: 2476 2724 2832 2905 3448 4337 5966 8 Size: 248 108 73 543 889 1629 43 Fragments arranged by size: 1629 1425 889 543 391 275 248 240 108 73 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 3448 1537 Size: 275 1636 4090 Fragments arranged by size: 4090 1636 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2816 3931 1353 Size: 1463 1115 3423 Fragments arranged by size: 3423 1463 1115 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4588 4962 5108 387 Size: 1064 138 2999 374 146 1280 Fragments arranged by size: 2999 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2393 2525 2962 2993 3131 4064 4661 Size: 393 761 132 437 31 138 933 597 Cuts at: 4661 5448 5716 1239 Size: 787 268 1524 Fragments arranged by size: 1524 933 787 761 597 437 393 268 138 132 31 EcoO109I rG'GnC_Cy Cuts at: 2284 2554 2555 2629 2284 Size: 270 1 74 5656 Fragments arranged by size: 5656 270 74 1 EcoRI G'AATT_C Cuts at: 3310 3688 3310 Size: 378 5623 FspI TGC'GCA Cuts at: 4507 4507 Size: 6001 HaeII r_GCGC'y Cuts at: 1678 5148 1678 Size: 3470 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4642 4674 4716 4748 1373 Size: 32 135 32 3070 32 42 32 2626 Fragments arranged by size: 3070 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3345 3591 669 Size: 988 281 1407 246 3079 Fragments arranged by size: 3079 1407 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6001 HpaI GTT'AAC Cuts at: 3591 3591 Size: 6001 KpnI G_GTAC'C Cuts at: 3326 3326 Size: 6001 MfeI C'AATT_G Cuts at: 3600 3600 Size: 6001 MluI A'CGCG_T Cuts at: 5877 5877 Size: 6001 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5115 5299 1305 Size: 3810 184 2007 Fragments arranged by size: 3810 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2478 10 Size: 54 1425 343 646 3533 Fragments arranged by size: 3533 1425 646 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3959 4318 4477 518 Size: 648 2793 359 159 2042 Fragments arranged by size: 2793 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2059 2075 2178 2344 2536 2730 2757 3078 Size: 453 16 103 166 192 194 27 321 Cuts at: 3078 3103 3417 4094 5242 5487 1606 Size: 25 314 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 453 321 314 245 194 192 166 103 27 25 16 NaeI GCC'GGC Cuts at: 2719 2719 Size: 6001 NcoI C'CATG_G Cuts at: 513 1065 2407 513 Size: 552 1342 4107 Fragments arranged by size: 4107 1342 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6001 NgoMIV G'CCGG_C Cuts at: 2717 2717 Size: 6001 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6001 NotI GC'GGCC_GC Cuts at: 3448 3448 Size: 6001 NspI r_CATG'y Cuts at: 1132 1229 3359 4904 1132 Size: 97 2130 1545 2229 Fragments arranged by size: 2229 2130 1545 97 PasI CC'CwG_GG Cuts at: 1348 2332 1348 Size: 984 5017 PciI A'CATG_T Cuts at: 4900 4900 Size: 6001 PflMI CCAn_nnn'nTGG Cuts at: 1108 2775 1108 Size: 1667 4334 PfoI T'CCnGG_A Cuts at: 2814 2988 2814 Size: 174 5827 PmeI GTTT'AAAC Cuts at: 3305 3305 Size: 6001 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4070 4102 5583 5615 4070 Size: 32 1481 32 4456 Fragments arranged by size: 4456 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2284 2629 2284 Size: 345 5656 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6001 PsiI TTA'TAA Cuts at: 3571 3571 Size: 6001 PspOMI G'GGCC_C Cuts at: 2554 2554 Size: 6001 PstI C_TGCA'G Cuts at: 830 3353 830 Size: 2523 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6001 PvuII CAG'CTG Cuts at: 2536 2757 3103 2536 Size: 221 346 5434 Fragments arranged by size: 5434 346 221 SacII CC_GC'GG Cuts at: 2076 2076 Size: 6001 SalI G'TCGA_C Cuts at: 1936 3343 1936 Size: 1407 4594 SbfI CC_TGCA'GG Cuts at: 3353 3353 Size: 6001 ScaI AGT'ACT Cuts at: 4249 5961 4249 Size: 1712 4289 SfcI C'TryA_G Cuts at: 826 1045 3349 4484 5165 5356 826 Size: 219 2304 1135 681 191 1471 Fragments arranged by size: 2304 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6001 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6001 SmaI CCC'GGG Cuts at: 1872 2034 3328 1872 Size: 162 1294 4545 Fragments arranged by size: 4545 1294 162 SmlI C'TyrA_G Cuts at: 820 1017 1943 2108 4105 5006 5268 820 Size: 197 926 165 1997 901 262 1553 Fragments arranged by size: 1997 1553 926 901 262 197 165 SnaBI TAC'GTA Cuts at: 493 3298 3792 493 Size: 2805 494 2702 Fragments arranged by size: 2805 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6001 SphI G_CATG'C Cuts at: 1132 3359 1132 Size: 2227 3774 SspI AAT'ATT Cuts at: 5 52 3925 5 Size: 47 3873 2081 Fragments arranged by size: 3873 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6001 StyI C'CwwG_G Cuts at: 513 1065 2100 2280 2407 3440 513 Size: 552 1035 180 127 1033 3074 Fragments arranged by size: 3074 1035 1033 552 180 127 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4028 4045 4198 4383 4828 325 Size: 1255 2448 17 153 185 445 1498 Fragments arranged by size: 2448 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2172 4247 5959 Size: 274 80 33 51 1443 195 2075 1712 Cuts at: 5959 96 Size: 138 Fragments arranged by size: 2075 1712 1443 274 195 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5961 5993 5961 Size: 32 5969 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6001 XbaI T'CTAG_A Cuts at: 3337 3337 Size: 6001 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6001 XmaI C'CCGG_G Cuts at: 1870 2032 3326 1870 Size: 162 1294 4545 Fragments arranged by size: 4545 1294 162 XmnI GAAnn'nnTTC Cuts at: 2201 4130 2201 Size: 1929 4072 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3809 276 Size: 53 83 186 936 2275 2468 Fragments arranged by size: 2468 2275 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI