(Circular) (Six-base) MAPSORT of: pFN21ASDA0273.seq Check: 9126 from: 1 to: 5719 pFN21ASDA0273 5719 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2078 2078 Size: 5719 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2193 3529 278 Size: 53 83 186 936 657 1336 2468 Fragments arranged by size: 2468 1336 936 657 186 83 53 AccI GT'mk_AC Cuts at: 1937 2898 2954 3062 1937 Size: 961 56 108 4594 Fragments arranged by size: 4594 961 108 56 Acc65I G'GTAC_C Cuts at: 3040 3040 Size: 5719 AclI AA'CG_TT Cuts at: 1563 3846 4219 1563 Size: 2283 373 3063 Fragments arranged by size: 3063 2283 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2375 2534 2557 3782 5166 5434 1632 Size: 743 159 23 1225 1384 268 1917 Fragments arranged by size: 1917 1384 1225 743 268 159 23 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5522 AflIII A'CryG_T Cuts at: 1203 4618 5595 1203 Size: 3415 977 1327 Fragments arranged by size: 3415 1327 977 AgeI A'CCGG_T Cuts at: 4550 4550 Size: 5719 AhdI GACnn_n'nnGTC Cuts at: 4448 4448 Size: 5719 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5719 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5719 AlwNI CAG_nnn'CTG Cuts at: 3462 5034 3462 Size: 1572 4147 ApaLI G'TGCA_C Cuts at: 3776 4932 3776 Size: 1156 4563 ApoI r'AATT_y Cuts at: 1493 2164 3028 3254 3406 5515 1493 Size: 671 864 226 152 2109 1697 Fragments arranged by size: 2109 1697 864 671 226 152 AseI AT'TA_AT Cuts at: 160 1032 4273 160 Size: 872 3241 1606 Fragments arranged by size: 3241 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5719 AvaI C'yCGr_G Cuts at: 1870 1943 3044 1870 Size: 73 1101 4545 Fragments arranged by size: 4545 1101 73 BamHI G'GATC_C Cuts at: 3049 3416 3049 Size: 367 5352 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2740 2912 2934 3040 4495 Size: 325 217 627 953 172 22 106 1455 Cuts at: 4495 618 Size: 1842 Fragments arranged by size: 1842 1455 953 627 325 217 172 106 22 BanII G_rGCy'C Cuts at: 1391 2261 2895 3038 1391 Size: 870 634 143 4072 Fragments arranged by size: 4072 870 634 143 BbeI G_GCGC'C Cuts at: 2744 2744 Size: 5719 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2431 2560 2839 928 Size: 971 532 129 279 3808 Fragments arranged by size: 3808 971 532 279 129 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3910 3944 1903 Size: 34 1973 34 3678 Fragments arranged by size: 3678 1973 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3611 4821 3611 Size: 1210 4509 BclI T'GATC_A Cuts at: 1548 1548 Size: 5719 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4330 136 Size: 107 122 71 631 605 2658 1525 Fragments arranged by size: 2658 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5714 5714 Size: 5719 BlpI GC'TnA_GC Cuts at: 2385 2403 2652 3136 2385 Size: 18 249 484 4968 Fragments arranged by size: 4968 484 249 18 Bme1580I G_kGCm'C Cuts at: 1790 2915 2937 3780 4936 1790 Size: 1125 22 843 1156 2573 Fragments arranged by size: 2573 1156 1125 843 22 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5719 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2367 3450 4408 Size: 416 53 83 399 24 950 1083 958 Cuts at: 4408 442 Size: 1753 Fragments arranged by size: 1753 1083 958 950 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5719 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2135 2353 2894 4379 1239 Size: 896 218 541 1485 2579 Fragments arranged by size: 2579 1485 896 541 218 Bpu10I CC'TnA_GC Cuts at: 2255 2255 Size: 5719 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3844 4709 5007 3844 Size: 865 298 4556 Fragments arranged by size: 4556 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4382 882 Size: 617 2883 2219 Fragments arranged by size: 2883 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3016 3510 493 Size: 711 1812 494 2702 Fragments arranged by size: 2702 1812 711 494 BsaBI GATnn'nnATC Cuts at: 5713 5713 Size: 5719 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2190 2741 3526 3908 Size: 53 83 186 936 657 551 785 382 Cuts at: 3908 4543 275 Size: 635 1451 Fragments arranged by size: 1451 936 785 657 635 551 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4152 4550 4824 4971 5382 4152 Size: 398 274 147 411 4489 Fragments arranged by size: 4489 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2018 2048 1178 Size: 30 165 30 615 30 4849 Fragments arranged by size: 4849 615 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5719 BseYI C'CCAG_C Cuts at: 2647 2669 2978 4922 5418 2647 Size: 22 309 1944 496 2948 Fragments arranged by size: 2948 1944 496 309 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2306 2338 1163 Size: 573 570 32 4544 Fragments arranged by size: 4544 573 570 32 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2629 2779 3169 3930 4958 1540 Size: 275 186 628 150 390 761 1028 2301 Fragments arranged by size: 2301 1028 761 628 390 275 186 150 BsiHKAI G_wGCw'C Cuts at: 2379 3780 3865 4936 2379 Size: 1401 85 1071 3162 Fragments arranged by size: 3162 1401 1071 85 BsmI GAATG_Cn' Cuts at: 3230 3323 3230 Size: 93 5626 BsmBI CGTCTCn'nnnn_ Cuts at: 2333 2333 Size: 5719 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2261 2379 2895 2915 2937 3038 3780 Size: 399 471 118 516 20 22 101 742 Cuts at: 3780 3865 4936 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 516 471 399 118 101 85 22 20 BspEI T'CCGG_A Cuts at: 5382 5382 Size: 5719 BspHI T'CATG_A Cuts at: 2943 3608 5338 2943 Size: 665 1730 3324 Fragments arranged by size: 3324 1730 665 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2078 2828 2999 3074 844 Size: 1234 750 171 75 3489 Fragments arranged by size: 3489 1234 750 171 75 BsrBI CCG'CTC Cuts at: 1394 1823 3606 1394 Size: 429 1783 3507 Fragments arranged by size: 3507 1783 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2796 4214 4388 65 Size: 1183 1548 1418 174 1396 Fragments arranged by size: 1548 1418 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4363 4550 1700 Size: 110 2553 187 2869 Fragments arranged by size: 2869 2553 187 110 BsrGI T'GTAC_A Cuts at: 96 2706 96 Size: 2610 3109 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5719 BssSI C'ACGA_G Cuts at: 2204 3779 4791 2204 Size: 1575 1012 3132 Fragments arranged by size: 3132 1575 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2991 794 Size: 2197 3522 BstBI TT'CG_AA Cuts at: 2007 4523 2007 Size: 2516 3203 BstEII G'GTnAC_C Cuts at: 1184 2122 1184 Size: 938 4781 BstXI CCAn_nnnn'nTGG Cuts at: 2206 2206 Size: 5719 BstYI r'GATC_y Cuts at: 1972 2643 3049 3416 3801 3818 5270 5356 5368 Size: 671 406 367 385 17 1452 86 12 Cuts at: 5368 5714 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 671 406 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2097 2533 2560 513 Size: 552 957 75 436 27 3672 Fragments arranged by size: 3672 957 552 436 75 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2322 487 Size: 683 56 1096 3884 Fragments arranged by size: 3884 1096 683 56 BtsI GCAGTG_nn' Cuts at: 2222 2223 2301 2485 3235 4029 4049 2222 Size: 1 78 184 750 794 20 3892 Fragments arranged by size: 3892 794 750 184 78 20 1 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5719 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5684 DraI TTT'AAA Cuts at: 3023 3023 Size: 5719 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4726 809 Size: 507 3410 1802 Fragments arranged by size: 3410 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2208 2280 2626 Size: 54 1425 50 275 18 378 72 346 Cuts at: 2626 3166 4055 5684 8 Size: 540 889 1629 43 Fragments arranged by size: 1629 1425 889 540 378 346 275 72 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2626 3166 1537 Size: 275 814 540 4090 Fragments arranged by size: 4090 814 540 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2666 3649 1353 Size: 1313 983 3423 Fragments arranged by size: 3423 1313 983 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2840 4306 4680 4826 387 Size: 1064 138 1251 1466 374 146 1280 Fragments arranged by size: 1466 1280 1251 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2135 2353 2375 2534 2557 2894 3782 Size: 393 503 218 22 159 23 337 888 Cuts at: 3782 4379 5166 5434 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 888 787 597 503 393 337 268 218 159 23 22 EcoO109I rG'GnC_Cy Cuts at: 2481 2863 2974 2481 Size: 382 111 5226 Fragments arranged by size: 5226 382 111 EcoRI G'AATT_C Cuts at: 2164 3028 3406 2164 Size: 864 378 4477 Fragments arranged by size: 4477 864 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2482 2482 Size: 5719 FspI TGC'GCA Cuts at: 4225 4225 Size: 5719 HaeII r_GCGC'y Cuts at: 1678 2638 2659 2744 4866 1678 Size: 960 21 85 2122 2531 Fragments arranged by size: 2531 2122 960 85 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2018 2050 2635 2667 4360 Size: 32 135 32 446 32 585 32 1693 Cuts at: 4360 4392 4434 4466 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1693 585 446 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2899 3063 3309 669 Size: 988 281 961 164 246 3079 Fragments arranged by size: 3079 988 961 281 246 164 HindIII A'AGCT_T Cuts at: 748 748 Size: 5719 HpaI GTT'AAC Cuts at: 3309 3309 Size: 5719 KasI G'GCGC_C Cuts at: 2740 2740 Size: 5719 KpnI G_GTAC'C Cuts at: 3044 3044 Size: 5719 MfeI C'AATT_G Cuts at: 3318 3318 Size: 5719 MluI A'CGCG_T Cuts at: 5595 5595 Size: 5719 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2193 4833 5017 1305 Size: 888 2640 184 2007 Fragments arranged by size: 2640 2007 888 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2210 10 Size: 54 1425 343 378 3519 Fragments arranged by size: 3519 1425 378 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2234 2732 3002 3677 4036 4195 518 Size: 648 1068 498 270 675 359 159 2042 Fragments arranged by size: 2042 1068 675 648 498 359 270 159 MspA1I CmG'CkG Cuts at: 1606 2526 2550 2577 2631 2982 3135 3812 4960 Size: 920 24 27 54 351 153 677 1148 Cuts at: 4960 5205 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 920 677 351 245 153 54 27 24 NarI GG'CG_CC Cuts at: 2741 2741 Size: 5719 NcoI C'CATG_G Cuts at: 513 1065 2022 2097 513 Size: 552 957 75 4135 Fragments arranged by size: 4135 957 552 75 NdeI CA'TA_TG Cuts at: 387 387 Size: 5719 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5719 NotI GC'GGCC_GC Cuts at: 3166 3166 Size: 5719 NspI r_CATG'y Cuts at: 1132 1229 2425 2477 2500 2707 3077 4622 1132 Size: 97 1196 52 23 207 370 1545 2229 Fragments arranged by size: 2229 1545 1196 370 207 97 52 23 PasI CC'CwG_GG Cuts at: 1348 2755 2908 2917 1348 Size: 1407 153 9 4150 Fragments arranged by size: 4150 1407 153 9 PciI A'CATG_T Cuts at: 4618 4618 Size: 5719 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5719 PfoI T'CCnGG_A Cuts at: 2790 2871 2790 Size: 81 5638 PmeI GTTT'AAAC Cuts at: 3023 3023 Size: 5719 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3788 3820 5301 5333 3788 Size: 32 1481 32 4174 Fragments arranged by size: 4174 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2481 2863 2974 2481 Size: 382 111 5226 Fragments arranged by size: 5226 382 111 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5719 PsiI TTA'TAA Cuts at: 3289 3289 Size: 5719 PstI C_TGCA'G Cuts at: 830 2505 2825 3071 830 Size: 1675 320 246 3478 Fragments arranged by size: 3478 1675 320 246 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5719 PvuII CAG'CTG Cuts at: 2577 2982 2577 Size: 405 5314 SalI G'TCGA_C Cuts at: 1936 2897 3061 1936 Size: 961 164 4594 Fragments arranged by size: 4594 961 164 SbfI CC_TGCA'GG Cuts at: 3071 3071 Size: 5719 ScaI AGT'ACT Cuts at: 2465 3967 5679 2465 Size: 1502 1712 2505 Fragments arranged by size: 2505 1712 1502 SexAI A'CCwGG_T Cuts at: 2319 2319 Size: 5719 SfcI C'TryA_G Cuts at: 826 1045 2501 2821 3067 4202 4883 5074 826 Size: 219 1456 320 246 1135 681 191 1471 Fragments arranged by size: 1471 1456 1135 681 320 246 219 191 SfoI GGC'GCC Cuts at: 2742 2742 Size: 5719 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5719 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5719 SmaI CCC'GGG Cuts at: 1872 3046 1872 Size: 1174 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3823 4724 4986 820 Size: 197 926 1880 901 262 1553 Fragments arranged by size: 1880 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3016 3510 493 Size: 2523 494 2702 Fragments arranged by size: 2702 2523 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5719 SphI G_CATG'C Cuts at: 1132 2425 2477 3077 1132 Size: 1293 52 600 3774 Fragments arranged by size: 3774 1293 600 52 SspI AAT'ATT Cuts at: 5 52 3643 5 Size: 47 3591 2081 Fragments arranged by size: 3591 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5719 StyI C'CwwG_G Cuts at: 513 1065 2022 2097 2211 2370 3158 513 Size: 552 957 75 114 159 788 3074 Fragments arranged by size: 3074 957 788 552 159 114 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2141 3746 3763 3916 4101 4546 325 Size: 1255 561 1605 17 153 185 445 1498 Fragments arranged by size: 1605 1498 1255 561 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2271 2463 2706 Size: 274 80 33 51 1443 294 192 243 Cuts at: 2706 3965 5677 96 Size: 1259 1712 138 Fragments arranged by size: 1712 1443 1259 294 274 243 192 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5679 5711 5679 Size: 32 5687 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5719 XbaI T'CTAG_A Cuts at: 3055 3055 Size: 5719 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2203 2377 2734 2752 2203 Size: 174 357 18 5170 Fragments arranged by size: 5170 357 174 18 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5719 XmaI C'CCGG_G Cuts at: 1870 3044 1870 Size: 1174 4545 XmnI GAAnn'nnTTC Cuts at: 3848 3848 Size: 5719 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2191 3527 276 Size: 53 83 186 936 657 1336 2468 Fragments arranged by size: 2468 1336 936 657 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI