(Circular) (Six-base) MAPSORT of: pFN21ASDA0269.seq Check: 1560 from: 1 to: 6406 pFN21ASDA0269 6406 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4216 278 Size: 53 83 186 936 2680 2468 Fragments arranged by size: 2680 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3749 1937 Size: 1812 4594 Acc65I G'GTAC_C Cuts at: 3727 3727 Size: 6406 AclI AA'CG_TT Cuts at: 1563 4533 4906 1563 Size: 2970 373 3063 Fragments arranged by size: 3063 2970 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2187 2197 2486 2685 3131 4469 5853 6121 Size: 555 10 289 199 446 1338 1384 268 Cuts at: 6121 1632 Size: 1917 Fragments arranged by size: 1917 1384 1338 555 446 289 268 199 10 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6209 AflIII A'CryG_T Cuts at: 1203 2236 5305 6282 1203 Size: 1033 3069 977 1327 Fragments arranged by size: 3069 1327 1033 977 AgeI A'CCGG_T Cuts at: 5237 5237 Size: 6406 AhdI GACnn_n'nnGTC Cuts at: 5135 5135 Size: 6406 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6406 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2387 1184 Size: 1203 5203 AlwNI CAG_nnn'CTG Cuts at: 2641 3104 3289 4149 5721 2641 Size: 463 185 860 1572 3326 Fragments arranged by size: 3326 1572 860 463 185 ApaLI G'TGCA_C Cuts at: 4463 5619 4463 Size: 1156 5250 ApoI r'AATT_y Cuts at: 1493 3674 3715 3941 4093 6202 1493 Size: 2181 41 226 152 2109 1697 Fragments arranged by size: 2181 2109 1697 226 152 41 AseI AT'TA_AT Cuts at: 160 1032 3428 4960 160 Size: 872 2396 1532 1606 Fragments arranged by size: 2396 1606 1532 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 AvaI C'yCGr_G Cuts at: 1870 1943 3731 1870 Size: 73 1788 4545 Fragments arranged by size: 4545 1788 73 AvrII C'CTAG_G Cuts at: 2051 2051 Size: 6406 BamHI G'GATC_C Cuts at: 3736 4103 3736 Size: 367 6039 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3727 5182 618 Size: 325 217 627 1940 1455 1842 Fragments arranged by size: 1940 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2987 3725 1391 Size: 1596 738 4072 Fragments arranged by size: 4072 1596 738 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5435 BbvCI CC'TCA_GC Cuts at: 2823 2823 Size: 6406 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4597 4631 1903 Size: 34 2660 34 3678 Fragments arranged by size: 3678 2660 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4298 5508 4298 Size: 1210 5196 BclI T'GATC_A Cuts at: 1548 1548 Size: 6406 BfrBI ATG'CAT Cuts at: 2875 2875 Size: 6406 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5017 136 Size: 107 122 71 631 605 3345 1525 Fragments arranged by size: 3345 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6401 6401 Size: 6406 BlpI GC'TnA_GC Cuts at: 3588 3823 3588 Size: 235 6171 Bme1580I G_kGCm'C Cuts at: 1790 4467 5623 1790 Size: 2677 1156 2573 Fragments arranged by size: 2677 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6406 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3230 4137 5095 Size: 416 53 83 399 24 1813 907 958 Cuts at: 5095 442 Size: 1753 Fragments arranged by size: 1813 1753 958 907 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6406 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3006 3006 Size: 6406 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3057 3081 3102 3120 3180 5066 1239 Size: 1818 24 21 18 60 1886 2579 Fragments arranged by size: 2579 1886 1818 60 24 21 18 Bpu10I CC'TnA_GC Cuts at: 2823 2823 Size: 6406 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4531 5396 5694 4531 Size: 865 298 5243 Fragments arranged by size: 5243 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5069 882 Size: 617 3570 2219 Fragments arranged by size: 3570 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2749 3703 4197 493 Size: 711 1545 954 494 2702 Fragments arranged by size: 2702 1545 954 711 494 BsaBI GATnn'nnATC Cuts at: 2676 6400 2676 Size: 3724 2682 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4213 4595 5230 275 Size: 53 83 186 936 2680 382 635 1451 Fragments arranged by size: 2680 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4839 5237 5511 5658 6069 4839 Size: 398 274 147 411 5176 Fragments arranged by size: 5176 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3046 3076 1178 Size: 30 165 30 1643 30 4508 Fragments arranged by size: 4508 1643 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2991 3054 3132 3168 3171 3186 3303 3432 Size: 1805 63 78 36 3 15 117 129 Cuts at: 3432 1186 Size: 4160 Fragments arranged by size: 4160 1805 129 117 78 63 36 15 3 BseYI C'CCAG_C Cuts at: 2713 3172 5609 6105 2713 Size: 459 2437 496 3014 Fragments arranged by size: 3014 2437 496 459 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5833 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2362 3856 4617 5645 1540 Size: 275 186 361 1494 761 1028 2301 Fragments arranged by size: 2301 1494 1028 761 361 275 186 BsiHKAI G_wGCw'C Cuts at: 2611 4467 4552 5623 2611 Size: 1856 85 1071 3394 Fragments arranged by size: 3394 1856 1071 85 BsiWI C'GTAC_G Cuts at: 2394 2394 Size: 6406 BsmI GAATG_Cn' Cuts at: 3330 3917 4010 3330 Size: 587 93 5726 Fragments arranged by size: 5726 587 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2384 2384 Size: 6406 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2611 2987 3725 4467 4552 5623 1391 Size: 399 821 376 738 742 85 1071 2174 Fragments arranged by size: 2174 1071 821 742 738 399 376 85 BspEI T'CCGG_A Cuts at: 6069 6069 Size: 6406 BspHI T'CATG_A Cuts at: 2584 2614 4295 6025 2584 Size: 30 1681 1730 2965 Fragments arranged by size: 2965 1730 1681 30 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3469 3761 844 Size: 2625 292 3489 Fragments arranged by size: 3489 2625 292 BsrBI CCG'CTC Cuts at: 1394 1823 4293 1394 Size: 429 2470 3507 Fragments arranged by size: 3507 2470 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2225 4901 5075 65 Size: 1183 977 2676 174 1396 Fragments arranged by size: 2676 1396 1183 977 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5050 5237 1700 Size: 110 3240 187 2869 Fragments arranged by size: 3240 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6406 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6406 BssSI C'ACGA_G Cuts at: 4466 5478 4466 Size: 1012 5394 BstAPI GCAn_nnn'nTGC Cuts at: 794 2888 794 Size: 2094 4312 BstBI TT'CG_AA Cuts at: 2007 5210 2007 Size: 3203 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6406 BstYI r'GATC_y Cuts at: 1972 2765 3736 4103 4488 4505 5957 6043 6055 Size: 793 971 367 385 17 1452 86 12 Cuts at: 6055 6401 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 971 793 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5854 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5667 Fragments arranged by size: 5667 683 56 BtsI GCAGTG_nn' Cuts at: 2072 3102 3922 4716 4736 2072 Size: 1030 820 794 20 3742 Fragments arranged by size: 3742 1030 820 794 20 ClaI AT'CG_AT Cuts at: 1554 2046 1554 Size: 492 5914 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6371 DraI TTT'AAA Cuts at: 3710 3710 Size: 6406 DraIII CAC_nnn'GTG Cuts at: 3275 3275 Size: 6406 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5413 809 Size: 507 4097 1802 Fragments arranged by size: 4097 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3853 4742 6371 Size: 54 1425 50 275 18 2023 889 1629 Cuts at: 6371 8 Size: 43 Fragments arranged by size: 2023 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3853 1537 Size: 275 2041 4090 Fragments arranged by size: 4090 2041 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3018 3150 3561 4336 1353 Size: 1665 132 411 775 3423 Fragments arranged by size: 3423 1665 775 411 132 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4993 5367 5513 387 Size: 1064 138 3404 374 146 1280 Fragments arranged by size: 3404 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2187 2197 2486 2685 3057 3081 3102 Size: 393 555 10 289 199 372 24 21 Cuts at: 3102 3120 3131 3180 4469 5066 5853 6121 1239 Size: 18 11 49 1289 597 787 268 1524 Fragments arranged by size: 1524 1289 787 597 555 393 372 289 268 199 49 24 21 18 11 10 EcoO109I rG'GnC_Cy Cuts at: 3413 3413 Size: 6406 EcoRI G'AATT_C Cuts at: 3715 4093 3715 Size: 378 6028 FspI TGC'GCA Cuts at: 4912 4912 Size: 6406 HaeII r_GCGC'y Cuts at: 1678 3481 5553 1678 Size: 1803 2072 2531 Fragments arranged by size: 2531 2072 1803 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2234 2266 2923 2955 3046 Size: 32 135 32 662 32 657 32 91 Cuts at: 3046 3078 5047 5079 5121 5153 1373 Size: 32 1969 32 42 32 2626 Fragments arranged by size: 2626 1969 662 657 135 91 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2221 3030 3750 3996 669 Size: 988 281 283 809 720 246 3079 Fragments arranged by size: 3079 988 809 720 283 281 246 HindIII A'AGCT_T Cuts at: 748 2319 748 Size: 1571 4835 HpaI GTT'AAC Cuts at: 3996 3996 Size: 6406 KpnI G_GTAC'C Cuts at: 3731 3731 Size: 6406 MfeI C'AATT_G Cuts at: 3186 4005 3186 Size: 819 5587 MluI A'CGCG_T Cuts at: 6282 6282 Size: 6406 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3416 3675 5520 5704 1305 Size: 2111 259 1845 184 2007 Fragments arranged by size: 2111 2007 1845 259 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4584 Fragments arranged by size: 4584 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2760 4364 4723 4882 518 Size: 648 1594 1604 359 159 2042 Fragments arranged by size: 2042 1604 1594 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3822 4499 5647 5892 1606 Size: 2216 677 1148 245 2120 Fragments arranged by size: 2216 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5854 NdeI CA'TA_TG Cuts at: 387 2757 387 Size: 2370 4036 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6406 NotI GC'GGCC_GC Cuts at: 3853 3853 Size: 6406 NsiI A_TGCA'T Cuts at: 2877 2877 Size: 6406 NspI r_CATG'y Cuts at: 1132 1229 3764 5309 1132 Size: 97 2535 1545 2229 Fragments arranged by size: 2535 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6406 PciI A'CATG_T Cuts at: 5305 5305 Size: 6406 PflMI CCAn_nnn'nTGG Cuts at: 1108 2876 1108 Size: 1768 4638 PmeI GTTT'AAAC Cuts at: 3710 3710 Size: 6406 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4475 4507 5988 6020 4475 Size: 32 1481 32 4861 Fragments arranged by size: 4861 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3413 3413 Size: 6406 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6406 PsiI TTA'TAA Cuts at: 3976 3976 Size: 6406 PstI C_TGCA'G Cuts at: 830 3758 830 Size: 2928 3478 PvuI CG_AT'CG Cuts at: 2001 2362 2001 Size: 361 6045 SalI G'TCGA_C Cuts at: 1936 3748 1936 Size: 1812 4594 SapI GCTCTTCn'nnn_ Cuts at: 3561 3561 Size: 6406 SbfI CC_TGCA'GG Cuts at: 3758 3758 Size: 6406 ScaI AGT'ACT Cuts at: 4654 6366 4654 Size: 1712 4694 SfcI C'TryA_G Cuts at: 826 1045 2922 2958 3754 4889 5570 5761 826 Size: 219 1877 36 796 1135 681 191 1471 Fragments arranged by size: 1877 1471 1135 796 681 219 191 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6406 SmaI CCC'GGG Cuts at: 1872 3733 1872 Size: 1861 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4510 5411 5673 820 Size: 197 926 2567 901 262 1553 Fragments arranged by size: 2567 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3703 4197 493 Size: 3210 494 2702 Fragments arranged by size: 3210 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6406 SphI G_CATG'C Cuts at: 1132 3764 1132 Size: 2632 3774 SspI AAT'ATT Cuts at: 5 52 2116 4330 5 Size: 47 2064 2214 2081 Fragments arranged by size: 2214 2081 2064 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6406 StyI C'CwwG_G Cuts at: 513 1065 2051 2121 2647 3845 513 Size: 552 986 70 526 1198 3074 Fragments arranged by size: 3074 1198 986 552 526 70 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3186 4433 4450 4603 4788 5233 325 Size: 1255 1606 1247 17 153 185 445 1498 Fragments arranged by size: 1606 1498 1255 1247 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3202 4652 6364 Size: 274 80 33 51 1443 1225 1450 1712 Cuts at: 6364 96 Size: 138 Fragments arranged by size: 1712 1450 1443 1225 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6366 6398 6366 Size: 32 6374 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6406 XbaI T'CTAG_A Cuts at: 2572 3742 2572 Size: 1170 5236 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2119 2119 Size: 6406 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6406 XmaI C'CCGG_G Cuts at: 1870 3731 1870 Size: 1861 4545 XmnI GAAnn'nnTTC Cuts at: 2321 4535 2321 Size: 2214 4192 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4214 276 Size: 53 83 186 936 2680 2468 Fragments arranged by size: 2680 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI