(Circular) (Six-base) MAPSORT of: pFN21ASDA0264.seq Check: 1342 from: 1 to: 5971 pFN21ASDA0264 5971 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3781 278 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2127 3314 1937 Size: 190 1187 4594 Fragments arranged by size: 4594 1187 190 Acc65I G'GTAC_C Cuts at: 2328 3292 2328 Size: 964 5007 AclI AA'CG_TT Cuts at: 1563 4098 4471 1563 Size: 2535 373 3063 Fragments arranged by size: 3063 2535 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2100 2593 2880 2895 2903 4034 5418 5686 Size: 468 493 287 15 8 1131 1384 268 Cuts at: 5686 1632 Size: 1917 Fragments arranged by size: 1917 1384 1131 493 468 287 268 15 8 AfeI AGC'GCT Cuts at: 2859 2859 Size: 5971 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5774 AflIII A'CryG_T Cuts at: 1203 4870 5847 1203 Size: 3667 977 1327 Fragments arranged by size: 3667 1327 977 AgeI A'CCGG_T Cuts at: 4802 4802 Size: 5971 AhdI GACnn_n'nnGTC Cuts at: 4700 4700 Size: 5971 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5971 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5971 AlwNI CAG_nnn'CTG Cuts at: 3157 3714 5286 3157 Size: 557 1572 3842 Fragments arranged by size: 3842 1572 557 ApaLI G'TGCA_C Cuts at: 4028 5184 4028 Size: 1156 4815 ApoI r'AATT_y Cuts at: 1493 2430 3280 3506 3658 5767 1493 Size: 937 850 226 152 2109 1697 Fragments arranged by size: 2109 1697 937 850 226 152 AseI AT'TA_AT Cuts at: 160 1032 4525 160 Size: 872 3493 1606 Fragments arranged by size: 3493 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 AvaI C'yCGr_G Cuts at: 1870 1943 2265 3296 1870 Size: 73 322 1031 4545 Fragments arranged by size: 4545 1031 322 73 BamHI G'GATC_C Cuts at: 3301 3668 3301 Size: 367 5604 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2328 3292 4747 618 Size: 325 217 627 541 964 1455 1842 Fragments arranged by size: 1842 1455 964 627 541 325 217 BanII G_rGCy'C Cuts at: 1391 2800 3290 1391 Size: 1409 490 4072 Fragments arranged by size: 4072 1409 490 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2606 2841 2943 928 Size: 971 707 235 102 3956 Fragments arranged by size: 3956 971 707 235 102 BbvCI CC'TCA_GC Cuts at: 2079 2079 Size: 5971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4162 4196 1903 Size: 34 2225 34 3678 Fragments arranged by size: 3678 2225 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3863 5073 3863 Size: 1210 4761 BclI T'GATC_A Cuts at: 1548 1548 Size: 5971 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4582 136 Size: 107 122 71 631 605 2910 1525 Fragments arranged by size: 2910 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5966 5966 Size: 5971 BlpI GC'TnA_GC Cuts at: 3388 3388 Size: 5971 Bme1580I G_kGCm'C Cuts at: 1790 4032 5188 1790 Size: 2242 1156 2573 Fragments arranged by size: 2573 2242 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5971 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2686 3702 4660 Size: 416 53 83 399 24 1269 1016 958 Cuts at: 4660 442 Size: 1753 Fragments arranged by size: 1753 1269 1016 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5971 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2876 2876 Size: 5971 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4631 1239 Size: 3392 2579 Bpu10I CC'TnA_GC Cuts at: 2079 2079 Size: 5971 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4096 4961 5259 4096 Size: 865 298 4808 Fragments arranged by size: 4808 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4634 882 Size: 617 3135 2219 Fragments arranged by size: 3135 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3268 3762 493 Size: 711 2064 494 2702 Fragments arranged by size: 2702 2064 711 494 BsaBI GATnn'nnATC Cuts at: 5965 5965 Size: 5971 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3778 4160 4795 275 Size: 53 83 186 936 2245 382 635 1451 Fragments arranged by size: 2245 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4404 4802 5076 5223 5634 4404 Size: 398 274 147 411 4741 Fragments arranged by size: 4741 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5746 Fragments arranged by size: 5746 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2636 2930 1186 Size: 1450 294 4227 Fragments arranged by size: 4227 1450 294 BseYI C'CCAG_C Cuts at: 3093 5174 5670 3093 Size: 2081 496 3394 Fragments arranged by size: 3394 2081 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5398 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3421 4182 5210 1540 Size: 275 186 1420 761 1028 2301 Fragments arranged by size: 2301 1420 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4032 4117 5188 4032 Size: 85 1071 4815 Fragments arranged by size: 4815 1071 85 BsmI GAATG_Cn' Cuts at: 3482 3575 3482 Size: 93 5878 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2800 3290 4032 4117 5188 1391 Size: 399 1010 490 742 85 1071 2174 Fragments arranged by size: 2174 1071 1010 742 490 399 85 BspEI T'CCGG_A Cuts at: 5634 5634 Size: 5971 BspHI T'CATG_A Cuts at: 2523 3860 5590 2523 Size: 1337 1730 2904 Fragments arranged by size: 2904 1730 1337 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2084 2115 3326 844 Size: 1240 31 1211 3489 Fragments arranged by size: 3489 1240 1211 31 BsrBI CCG'CTC Cuts at: 1394 1823 3858 1394 Size: 429 2035 3507 Fragments arranged by size: 3507 2035 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2584 4466 4640 65 Size: 1183 1336 1882 174 1396 Fragments arranged by size: 1882 1396 1336 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4615 4802 1700 Size: 110 2805 187 2869 Fragments arranged by size: 2869 2805 187 110 BsrGI T'GTAC_A Cuts at: 96 3181 96 Size: 3085 2886 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5971 BssSI C'ACGA_G Cuts at: 4031 5043 4031 Size: 1012 4959 BstAPI GCAn_nnn'nTGC Cuts at: 794 3157 794 Size: 2363 3608 BstBI TT'CG_AA Cuts at: 2007 4775 2007 Size: 2768 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5971 BstXI CCAn_nnnn'nTGG Cuts at: 2699 2699 Size: 5971 BstYI r'GATC_y Cuts at: 1972 3301 3668 4053 4070 5522 5608 5620 5966 Size: 1329 367 385 17 1452 86 12 346 Cuts at: 5966 1972 Size: 1977 Fragments arranged by size: 1977 1452 1329 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4462 Fragments arranged by size: 4462 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3121 487 Size: 683 56 1895 3337 Fragments arranged by size: 3337 1895 683 56 BtsI GCAGTG_nn' Cuts at: 2372 2883 3170 3487 4281 4301 2372 Size: 511 287 317 794 20 4042 Fragments arranged by size: 4042 794 511 317 287 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5971 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3079 3114 529 Size: 35 2515 35 3386 Fragments arranged by size: 3386 2515 35 35 DraI TTT'AAA Cuts at: 3275 3275 Size: 5971 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4978 809 Size: 507 3662 1802 Fragments arranged by size: 3662 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3418 4307 5936 Size: 54 1425 50 275 18 1588 889 1629 Cuts at: 5936 8 Size: 43 Fragments arranged by size: 1629 1588 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3418 1537 Size: 275 1606 4090 Fragments arranged by size: 4090 1606 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2265 2613 3901 1353 Size: 912 348 1288 3423 Fragments arranged by size: 3423 1288 912 348 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4558 4932 5078 387 Size: 1064 138 2969 374 146 1280 Fragments arranged by size: 2969 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2100 2593 2880 2895 2903 4034 4631 Size: 393 468 493 287 15 8 1131 597 Cuts at: 4631 5418 5686 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1131 787 597 493 468 393 287 268 15 8 EcoNI CCTnn'n_nnAGG Cuts at: 2359 2359 Size: 5971 EcoRI G'AATT_C Cuts at: 3280 3658 3280 Size: 378 5593 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2090 2623 2090 Size: 533 5438 FspI TGC'GCA Cuts at: 4477 4477 Size: 5971 HaeII r_GCGC'y Cuts at: 1678 2861 5118 1678 Size: 1183 2257 2531 Fragments arranged by size: 2531 2257 1183 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2168 2200 2489 2521 2906 Size: 32 135 32 596 32 289 32 385 Cuts at: 2906 2938 4612 4644 4686 4718 1373 Size: 32 1674 32 42 32 2626 Fragments arranged by size: 2626 1674 596 385 289 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3315 3561 669 Size: 988 281 1377 246 3079 Fragments arranged by size: 3079 1377 988 281 246 HindIII A'AGCT_T Cuts at: 748 2999 3041 748 Size: 2251 42 3678 Fragments arranged by size: 3678 2251 42 HpaI GTT'AAC Cuts at: 3561 3561 Size: 5971 KpnI G_GTAC'C Cuts at: 2332 3296 2332 Size: 964 5007 MfeI C'AATT_G Cuts at: 3570 3570 Size: 5971 MluI A'CGCG_T Cuts at: 5847 5847 Size: 5971 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5085 5269 1305 Size: 3780 184 2007 Fragments arranged by size: 3780 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4149 Fragments arranged by size: 4149 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3929 4288 4447 518 Size: 648 2763 359 159 2042 Fragments arranged by size: 2763 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2900 3387 4064 5212 5457 1606 Size: 1294 487 677 1148 245 2120 Fragments arranged by size: 2120 1294 1148 677 487 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4462 Fragments arranged by size: 4462 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5971 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5971 NotI GC'GGCC_GC Cuts at: 3418 3418 Size: 5971 NspI r_CATG'y Cuts at: 1132 1229 2764 3329 4874 1132 Size: 97 1535 565 1545 2229 Fragments arranged by size: 2229 1545 1535 565 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5971 PciI A'CATG_T Cuts at: 4870 4870 Size: 5971 PflMI CCAn_nnn'nTGG Cuts at: 1108 2325 1108 Size: 1217 4754 PmeI GTTT'AAAC Cuts at: 3275 3275 Size: 5971 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4040 4072 5553 5585 4040 Size: 32 1481 32 4426 Fragments arranged by size: 4426 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5971 PsiI TTA'TAA Cuts at: 3541 3541 Size: 5971 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2403 2435 2403 Size: 32 5939 PstI C_TGCA'G Cuts at: 830 2095 3162 3323 830 Size: 1265 1067 161 3478 Fragments arranged by size: 3478 1265 1067 161 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5971 PvuII CAG'CTG Cuts at: 2900 2900 Size: 5971 SalI G'TCGA_C Cuts at: 1936 3313 1936 Size: 1377 4594 SbfI CC_TGCA'GG Cuts at: 2095 3323 2095 Size: 1228 4743 ScaI AGT'ACT Cuts at: 4219 5931 4219 Size: 1712 4259 SfcI C'TryA_G Cuts at: 826 1045 2091 3158 3319 4454 5135 5326 826 Size: 219 1046 1067 161 1135 681 191 1471 Fragments arranged by size: 1471 1135 1067 1046 681 219 191 161 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5971 SmaI CCC'GGG Cuts at: 1872 3298 1872 Size: 1426 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4075 4976 5238 820 Size: 197 926 2132 901 262 1553 Fragments arranged by size: 2132 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3268 3762 493 Size: 2775 494 2702 Fragments arranged by size: 2775 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5971 SphI G_CATG'C Cuts at: 1132 3329 1132 Size: 2197 3774 SspI AAT'ATT Cuts at: 5 52 3895 5 Size: 47 3843 2081 Fragments arranged by size: 3843 2081 47 StuI AGG'CCT Cuts at: 1517 2658 3029 1517 Size: 1141 371 4459 Fragments arranged by size: 4459 1141 371 StyI C'CwwG_G Cuts at: 513 1065 2022 3410 513 Size: 552 957 1388 3074 Fragments arranged by size: 3074 1388 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2570 3107 3998 4015 4168 4353 4798 Size: 1255 990 537 891 17 153 185 445 Cuts at: 4798 325 Size: 1498 Fragments arranged by size: 1498 1255 990 891 537 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3181 4217 5929 Size: 274 80 33 51 1443 1204 1036 1712 Cuts at: 5929 96 Size: 138 Fragments arranged by size: 1712 1443 1204 1036 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2620 2652 5931 5963 2620 Size: 32 3279 32 2628 Fragments arranged by size: 3279 2628 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5971 XbaI T'CTAG_A Cuts at: 3172 3307 3172 Size: 135 5836 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5971 XmaI C'CCGG_G Cuts at: 1870 3296 1870 Size: 1426 4545 XmnI GAAnn'nnTTC Cuts at: 4100 4100 Size: 5971 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3779 276 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI