(Circular) (Six-base) MAPSORT of: pFN21ASDA0262.seq Check: 4686 from: 1 to: 7162 pFN21ASDA0262 7162 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3028 3028 Size: 7162 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4972 278 Size: 53 83 186 936 3436 2468 Fragments arranged by size: 3436 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2967 4505 1937 Size: 1030 1538 4594 Fragments arranged by size: 4594 1538 1030 Acc65I G'GTAC_C Cuts at: 4483 4483 Size: 7162 AclI AA'CG_TT Cuts at: 1563 5289 5662 1563 Size: 3726 373 3063 Fragments arranged by size: 3726 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2616 4300 4350 4401 5225 6609 6877 1632 Size: 984 1684 50 51 824 1384 268 1917 Fragments arranged by size: 1917 1684 1384 984 824 268 51 50 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6965 AflIII A'CryG_T Cuts at: 1203 6061 7038 1203 Size: 4858 977 1327 Fragments arranged by size: 4858 1327 977 AgeI A'CCGG_T Cuts at: 5993 5993 Size: 7162 AhdI GACnn_n'nnGTC Cuts at: 5891 5891 Size: 7162 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7162 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3966 1184 Size: 2782 4380 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2066 2098 2066 Size: 32 7130 AlwNI CAG_nnn'CTG Cuts at: 3043 3088 3389 3401 3653 3747 4168 4905 6477 Size: 45 301 12 252 94 421 737 1572 Cuts at: 6477 3043 Size: 3728 Fragments arranged by size: 3728 1572 737 421 301 252 94 45 12 ApaI G_GGCC'C Cuts at: 4021 4021 Size: 7162 ApaLI G'TGCA_C Cuts at: 5219 6375 5219 Size: 1156 6006 ApoI r'AATT_y Cuts at: 1493 2455 4309 4471 4697 4849 6958 1493 Size: 962 1854 162 226 152 2109 1697 Fragments arranged by size: 2109 1854 1697 962 226 162 152 AseI AT'TA_AT Cuts at: 160 1032 5716 160 Size: 872 4684 1606 Fragments arranged by size: 4684 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7162 AvaI C'yCGr_G Cuts at: 1870 1943 3110 3153 3635 3846 3929 4487 1870 Size: 73 1167 43 482 211 83 558 4545 Fragments arranged by size: 4545 1167 558 482 211 83 73 43 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2625 2658 2625 Size: 33 7129 BamHI G'GATC_C Cuts at: 2198 4492 4859 2198 Size: 2294 367 4501 Fragments arranged by size: 4501 2294 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4483 5938 618 Size: 325 217 627 2696 1455 1842 Fragments arranged by size: 2696 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2036 3147 3635 4021 4481 1391 Size: 645 1111 488 386 460 4072 Fragments arranged by size: 4072 1111 645 488 460 386 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2371 2604 2801 4143 928 Size: 971 472 233 197 1342 3947 Fragments arranged by size: 3947 1342 971 472 233 197 BbvCI CC'TCA_GC Cuts at: 3521 4169 3521 Size: 648 6514 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4423 4457 5353 5387 1903 Size: 34 2486 34 896 34 3678 Fragments arranged by size: 3678 2486 896 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5054 6264 5054 Size: 1210 5952 BclI T'GATC_A Cuts at: 1548 1548 Size: 7162 BfrBI ATG'CAT Cuts at: 2766 2766 Size: 7162 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5773 136 Size: 107 122 71 631 605 4101 1525 Fragments arranged by size: 4101 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3642 7157 3642 Size: 3515 3647 BlpI GC'TnA_GC Cuts at: 2030 4579 2030 Size: 2549 4613 Bme1580I G_kGCm'C Cuts at: 1790 4021 4299 5223 6379 1790 Size: 2231 278 924 1156 2573 Fragments arranged by size: 2573 2231 1156 924 278 BmgBI CAC'GTC Cuts at: 1318 2799 1318 Size: 1481 5681 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2407 2656 4293 Size: 416 53 83 399 24 990 249 1637 Cuts at: 4293 4893 5851 442 Size: 600 958 1753 Fragments arranged by size: 1753 1637 990 958 600 416 399 249 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7162 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3661 3817 3661 Size: 156 7006 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2176 2257 3080 3305 3644 5822 1239 Size: 937 81 823 225 339 2178 2579 Fragments arranged by size: 2579 2178 937 823 339 225 81 Bpu10I CC'TnA_GC Cuts at: 3521 4169 3521 Size: 648 6514 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2831 3131 5287 6152 6450 2831 Size: 300 2156 865 298 3543 Fragments arranged by size: 3543 2156 865 300 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3781 5825 882 Size: 617 2282 2044 2219 Fragments arranged by size: 2282 2219 2044 617 BsaAI yAC'GTr Cuts at: 493 1204 4459 4953 493 Size: 711 3255 494 2702 Fragments arranged by size: 3255 2702 711 494 BsaBI GATnn'nnATC Cuts at: 3447 7156 3447 Size: 3709 3453 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4969 5351 5986 275 Size: 53 83 186 936 3436 382 635 1451 Fragments arranged by size: 3436 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5595 5993 6267 6414 6825 5595 Size: 398 274 147 411 5932 Fragments arranged by size: 5932 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2066 2078 2096 2108 2794 Size: 30 165 30 663 12 18 12 686 Cuts at: 2794 2824 3278 3308 4407 4437 1178 Size: 30 454 30 1099 30 3903 Fragments arranged by size: 3903 1099 686 663 454 165 30 30 30 30 30 18 12 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2083 2301 3077 3110 3389 3643 3899 3964 Size: 897 218 776 33 279 254 256 65 Cuts at: 3964 4023 1186 Size: 59 4325 Fragments arranged by size: 4325 897 776 279 256 254 218 65 59 33 BseYI C'CCAG_C Cuts at: 2514 2757 6365 6861 2514 Size: 243 3608 496 2815 Fragments arranged by size: 3608 2815 496 243 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2756 3029 3563 1163 Size: 573 1020 273 534 4762 Fragments arranged by size: 4762 1020 573 534 273 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4612 5373 6401 1540 Size: 275 186 2611 761 1028 2301 Fragments arranged by size: 2611 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2036 3147 5223 5308 6379 2036 Size: 1111 2076 85 1071 2819 Fragments arranged by size: 2819 2076 1111 1071 85 BsmI GAATG_Cn' Cuts at: 4673 4766 4673 Size: 93 7069 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 3147 3635 4021 4299 4481 5223 Size: 399 246 1111 488 386 278 182 742 Cuts at: 5223 5308 6379 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1111 1071 742 488 399 386 278 246 182 85 BspEI T'CCGG_A Cuts at: 6825 6825 Size: 7162 BspHI T'CATG_A Cuts at: 4341 5051 6781 4341 Size: 710 1730 4722 Fragments arranged by size: 4722 1730 710 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3028 3977 4517 844 Size: 2184 949 540 3489 Fragments arranged by size: 3489 2184 949 540 BsrBI CCG'CTC Cuts at: 1394 1823 2136 5049 1394 Size: 429 313 2913 3507 Fragments arranged by size: 3507 2913 429 313 BsrDI GCAATG_nn' Cuts at: 65 1248 5657 5831 65 Size: 1183 4409 174 1396 Fragments arranged by size: 4409 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2156 5806 5993 1700 Size: 110 346 3650 187 2869 Fragments arranged by size: 3650 2869 346 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7162 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7162 BssSI C'ACGA_G Cuts at: 3602 5222 6234 3602 Size: 1620 1012 4530 Fragments arranged by size: 4530 1620 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3043 794 Size: 2249 4913 BstBI TT'CG_AA Cuts at: 2007 5966 2007 Size: 3959 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7162 BstXI CCAn_nnnn'nTGG Cuts at: 2420 2420 Size: 7162 BstYI r'GATC_y Cuts at: 1972 2198 2842 3642 4369 4492 4859 5244 5261 Size: 226 644 800 727 123 367 385 17 Cuts at: 5261 6713 6799 6811 7157 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 800 727 644 385 367 346 226 123 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3402 3402 Size: 7162 BtgI C'CryG_G Cuts at: 513 1065 2473 3315 4012 513 Size: 552 1408 842 697 3663 Fragments arranged by size: 3663 1408 842 697 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2193 487 Size: 683 56 967 5456 Fragments arranged by size: 5456 967 683 56 BtsI GCAGTG_nn' Cuts at: 2506 3390 4089 4678 5472 5492 2506 Size: 884 699 589 794 20 4176 Fragments arranged by size: 4176 884 794 699 589 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7162 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3639 3674 529 Size: 35 3075 35 4017 Fragments arranged by size: 4017 3075 35 35 DraI TTT'AAA Cuts at: 4466 4466 Size: 7162 DraIII CAC_nnn'GTG Cuts at: 2789 3586 2789 Size: 797 6365 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4081 6169 809 Size: 507 2765 2088 1802 Fragments arranged by size: 2765 2088 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2476 3177 4609 Size: 54 1425 50 275 18 646 701 1432 Cuts at: 4609 5498 7127 8 Size: 889 1629 43 Fragments arranged by size: 1629 1432 1425 889 701 646 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4609 1537 Size: 275 2797 4090 Fragments arranged by size: 4090 2797 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2107 2353 2909 3153 3366 3812 4377 5092 Size: 754 246 556 244 213 446 565 715 Cuts at: 5092 1353 Size: 3423 Fragments arranged by size: 3423 754 715 565 556 446 246 244 213 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2086 2128 5749 6123 6269 387 Size: 1064 138 497 42 3621 374 146 1280 Fragments arranged by size: 3621 1280 1064 497 374 146 138 42 EcoICRI GAG'CTC Cuts at: 2034 3145 2034 Size: 1111 6051 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2176 2257 2616 3080 3305 3644 4300 Size: 393 544 81 359 464 225 339 656 Cuts at: 4300 4350 4401 5225 5822 6609 6877 1239 Size: 50 51 824 597 787 268 1524 Fragments arranged by size: 1524 824 787 656 597 544 464 393 359 339 268 225 81 51 50 EcoO109I rG'GnC_Cy Cuts at: 3992 4017 4018 3992 Size: 25 1 7136 Fragments arranged by size: 7136 25 1 EcoRI G'AATT_C Cuts at: 4471 4849 4471 Size: 378 6784 EcoRV GAT'ATC Cuts at: 3710 3710 Size: 7162 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2310 2578 3526 2310 Size: 268 948 5946 Fragments arranged by size: 5946 948 268 FspI TGC'GCA Cuts at: 2669 5668 2669 Size: 2999 4163 FspAI rTGC'GCAy Cuts at: 2669 2669 Size: 7162 HaeII r_GCGC'y Cuts at: 1678 3835 6309 1678 Size: 2157 2474 2531 Fragments arranged by size: 2531 2474 2157 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2296 2328 2794 2826 3152 Size: 32 135 32 724 32 466 32 326 Cuts at: 3152 3184 3278 3310 3811 3843 4361 4393 5803 Size: 32 94 32 501 32 518 32 1410 Cuts at: 5803 5835 5877 5909 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1410 724 518 501 466 326 135 94 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2644 4506 4752 669 Size: 988 281 706 1862 246 3079 Fragments arranged by size: 3079 1862 988 706 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7162 HpaI GTT'AAC Cuts at: 2644 4752 2644 Size: 2108 5054 KpnI G_GTAC'C Cuts at: 4487 4487 Size: 7162 MfeI C'AATT_G Cuts at: 4761 4761 Size: 7162 MluI A'CGCG_T Cuts at: 7038 7038 Size: 7162 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2085 2109 4156 6276 6460 1305 Size: 780 24 2047 2120 184 2007 Fragments arranged by size: 2120 2047 2007 780 184 24 MscI TGG'CCA Cuts at: 10 64 1489 1832 2478 10 Size: 54 1425 343 646 4694 Fragments arranged by size: 4694 1425 646 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2877 2900 5120 5479 5638 518 Size: 648 1711 23 2220 359 159 2042 Fragments arranged by size: 2220 2042 1711 648 359 159 23 MspA1I CmG'CkG Cuts at: 1606 2029 2079 2908 3088 4578 5255 6403 6648 Size: 423 50 829 180 1490 677 1148 245 Cuts at: 6648 1606 Size: 2120 Fragments arranged by size: 2120 1490 1148 829 677 423 245 180 50 NaeI GCC'GGC Cuts at: 2158 2158 Size: 7162 NcoI C'CATG_G Cuts at: 513 1065 4012 513 Size: 552 2947 3663 Fragments arranged by size: 3663 2947 552 NdeI CA'TA_TG Cuts at: 387 3570 387 Size: 3183 3979 NgoMIV G'CCGG_C Cuts at: 2156 2156 Size: 7162 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7162 NotI GC'GGCC_GC Cuts at: 4609 4609 Size: 7162 NsiI A_TGCA'T Cuts at: 2768 2768 Size: 7162 NspI r_CATG'y Cuts at: 1132 1229 2766 4520 6065 1132 Size: 97 1537 1754 1545 2229 Fragments arranged by size: 2229 1754 1545 1537 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7162 PciI A'CATG_T Cuts at: 6061 6061 Size: 7162 PflMI CCAn_nnn'nTGG Cuts at: 1108 2587 1108 Size: 1479 5683 PfoI T'CCnGG_A Cuts at: 3138 3138 Size: 7162 PmeI GTTT'AAAC Cuts at: 4466 4466 Size: 7162 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2066 2098 5231 5263 6744 6776 2066 Size: 32 3133 32 1481 32 2452 Fragments arranged by size: 3133 2452 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3992 3992 Size: 7162 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7162 PsiI TTA'TAA Cuts at: 2211 4732 2211 Size: 2521 4641 PspOMI G'GGCC_C Cuts at: 4017 4017 Size: 7162 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2550 2582 2550 Size: 32 7130 PstI C_TGCA'G Cuts at: 830 2725 4514 830 Size: 1895 1789 3478 Fragments arranged by size: 3478 1895 1789 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7162 PvuII CAG'CTG Cuts at: 2908 3088 2908 Size: 180 6982 SacI G_AGCT'C Cuts at: 2036 3147 2036 Size: 1111 6051 SalI G'TCGA_C Cuts at: 1936 4504 1936 Size: 2568 4594 SanDI GG'GwC_CC Cuts at: 3992 3992 Size: 7162 SapI GCTCTTCn'nnn_ Cuts at: 2353 2353 Size: 7162 SbfI CC_TGCA'GG Cuts at: 4514 4514 Size: 7162 ScaI AGT'ACT Cuts at: 3008 5410 7122 3008 Size: 2402 1712 3048 Fragments arranged by size: 3048 2402 1712 SfcI C'TryA_G Cuts at: 826 1045 2721 3938 4510 5645 6326 6517 826 Size: 219 1676 1217 572 1135 681 191 1471 Fragments arranged by size: 1676 1471 1217 1135 681 572 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7162 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7162 SmaI CCC'GGG Cuts at: 1872 4489 1872 Size: 2617 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2846 3146 3929 5266 6167 6429 Size: 197 926 903 300 783 1337 901 262 Cuts at: 6429 820 Size: 1553 Fragments arranged by size: 1553 1337 926 903 901 783 300 262 197 SnaBI TAC'GTA Cuts at: 493 4459 4953 493 Size: 3966 494 2702 Fragments arranged by size: 3966 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7162 SphI G_CATG'C Cuts at: 1132 2766 4520 1132 Size: 1634 1754 3774 Fragments arranged by size: 3774 1754 1634 SspI AAT'ATT Cuts at: 5 52 5086 5 Size: 47 5034 2081 Fragments arranged by size: 5034 2081 47 StuI AGG'CCT Cuts at: 1517 3419 1517 Size: 1902 5260 StyI C'CwwG_G Cuts at: 513 1065 3243 4012 4601 513 Size: 552 2178 769 589 3074 Fragments arranged by size: 3074 2178 769 589 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4102 5189 5206 5359 5544 5989 325 Size: 1255 2522 1087 17 153 185 445 1498 Fragments arranged by size: 2522 1498 1255 1087 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3006 5408 7120 Size: 274 80 33 51 1443 1029 2402 1712 Cuts at: 7120 96 Size: 138 Fragments arranged by size: 2402 1712 1443 1029 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7122 7154 7122 Size: 32 7130 Tth111I GACn'n_nGTC Cuts at: 1316 3699 1316 Size: 2383 4779 XbaI T'CTAG_A Cuts at: 3344 4498 3344 Size: 1154 6008 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3019 3557 3019 Size: 538 6624 XhoI C'TCGA_G Cuts at: 1943 3929 1943 Size: 1986 5176 XmaI C'CCGG_G Cuts at: 1870 4487 1870 Size: 2617 4545 XmnI GAAnn'nnTTC Cuts at: 5291 5291 Size: 7162 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4970 276 Size: 53 83 186 936 3436 2468 Fragments arranged by size: 3436 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BbeI BsiWI BsmBI BstZ17I EcoNI FseI KasI NarI NruI PacI PmlI PspXI RsrII SacII SexAI SfiI SfoI SrfI SwaI