(Circular) (Six-base) MAPSORT of: pFN21ASDA0258.seq Check: 1827 from: 1 to: 5902 pFN21ASDA0258 5902 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3712 278 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2427 3096 3245 1937 Size: 490 669 149 4594 Fragments arranged by size: 4594 669 490 149 Acc65I G'GTAC_C Cuts at: 3223 3223 Size: 5902 AclI AA'CG_TT Cuts at: 1563 4029 4402 1563 Size: 2466 373 3063 Fragments arranged by size: 3063 2466 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2498 3088 3965 5349 5617 1632 Size: 866 590 877 1384 268 1917 Fragments arranged by size: 1917 1384 877 866 590 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5705 AflIII A'CryG_T Cuts at: 1203 2869 4801 5778 1203 Size: 1666 1932 977 1327 Fragments arranged by size: 1932 1666 1327 977 AgeI A'CCGG_T Cuts at: 4733 4733 Size: 5902 AhdI GACnn_n'nnGTC Cuts at: 4631 4631 Size: 5902 AleI CACnn'nnGTG Cuts at: 1166 2274 1166 Size: 1108 4794 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5902 AlwNI CAG_nnn'CTG Cuts at: 3080 3645 5217 3080 Size: 565 1572 3765 Fragments arranged by size: 3765 1572 565 ApaLI G'TGCA_C Cuts at: 3959 5115 3959 Size: 1156 4746 ApoI r'AATT_y Cuts at: 1493 2313 3002 3211 3437 3589 5698 1493 Size: 820 689 209 226 152 2109 1697 Fragments arranged by size: 2109 1697 820 689 226 209 152 AseI AT'TA_AT Cuts at: 160 1032 4456 160 Size: 872 3424 1606 Fragments arranged by size: 3424 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 AvaI C'yCGr_G Cuts at: 1870 1943 3015 3227 1870 Size: 73 1072 212 4545 Fragments arranged by size: 4545 1072 212 73 BamHI G'GATC_C Cuts at: 3232 3599 3232 Size: 367 5535 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2886 3223 4678 618 Size: 325 217 627 1099 337 1455 1842 Fragments arranged by size: 1842 1455 1099 627 337 325 217 BanII G_rGCy'C Cuts at: 1391 2046 3221 1391 Size: 655 1175 4072 Fragments arranged by size: 4072 1175 655 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4931 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4093 4127 1903 Size: 34 2156 34 3678 Fragments arranged by size: 3678 2156 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2303 3794 5004 2303 Size: 1491 1210 3201 Fragments arranged by size: 3201 1491 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5902 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4513 136 Size: 107 122 71 631 605 2841 1525 Fragments arranged by size: 2841 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5897 5897 Size: 5902 BlpI GC'TnA_GC Cuts at: 2045 2607 3319 2045 Size: 562 712 4628 Fragments arranged by size: 4628 712 562 Bme1580I G_kGCm'C Cuts at: 1790 2164 3963 5119 1790 Size: 374 1799 1156 2573 Fragments arranged by size: 2573 1799 1156 374 BmgBI CAC'GTC Cuts at: 1318 2737 2840 1318 Size: 1419 103 4380 Fragments arranged by size: 4380 1419 103 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3633 4591 442 Size: 416 53 83 399 24 2216 958 1753 Fragments arranged by size: 2216 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2606 1056 Size: 1550 4352 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2102 2364 4562 1239 Size: 863 262 2198 2579 Fragments arranged by size: 2579 2198 863 262 Bpu10I CC'TnA_GC Cuts at: 3081 3081 Size: 5902 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4027 4892 5190 4027 Size: 865 298 4739 Fragments arranged by size: 4739 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4565 882 Size: 617 3066 2219 Fragments arranged by size: 3066 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3199 3693 493 Size: 711 1995 494 2702 Fragments arranged by size: 2702 1995 711 494 BsaBI GATnn'nnATC Cuts at: 5896 5896 Size: 5902 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3709 4091 4726 275 Size: 53 83 186 936 2176 382 635 1451 Fragments arranged by size: 2176 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2523 4335 4733 5007 5154 5565 2523 Size: 1812 398 274 147 411 2860 Fragments arranged by size: 2860 1812 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5677 Fragments arranged by size: 5677 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2202 2585 2822 1186 Size: 1016 383 237 4266 Fragments arranged by size: 4266 1016 383 237 BseYI C'CCAG_C Cuts at: 2069 2615 3108 3173 5105 5601 2069 Size: 546 493 65 1932 496 2370 Fragments arranged by size: 2370 1932 546 496 493 65 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5329 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3352 4113 5141 1540 Size: 275 186 1351 761 1028 2301 Fragments arranged by size: 2301 1351 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2046 3963 4048 5119 2046 Size: 1917 85 1071 2829 Fragments arranged by size: 2829 1917 1071 85 BsmI GAATG_Cn' Cuts at: 3413 3506 3413 Size: 93 5809 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2164 3221 3963 4048 5119 1391 Size: 399 256 118 1057 742 85 1071 2174 Fragments arranged by size: 2174 1071 1057 742 399 256 118 85 BspEI T'CCGG_A Cuts at: 5565 5565 Size: 5902 BspHI T'CATG_A Cuts at: 3791 5521 3791 Size: 1730 4172 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3123 3257 844 Size: 2279 134 3489 Fragments arranged by size: 3489 2279 134 BsrBI CCG'CTC Cuts at: 1394 1823 3789 1394 Size: 429 1966 3507 Fragments arranged by size: 3507 1966 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4397 4571 65 Size: 1183 3149 174 1396 Fragments arranged by size: 3149 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4546 4733 1700 Size: 110 2736 187 2869 Fragments arranged by size: 2869 2736 187 110 BsrGI T'GTAC_A Cuts at: 96 2718 2815 2963 96 Size: 2622 97 148 3035 Fragments arranged by size: 3035 2622 148 97 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5902 BssSI C'ACGA_G Cuts at: 3962 4974 3962 Size: 1012 4890 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5902 BstBI TT'CG_AA Cuts at: 2007 4706 2007 Size: 2699 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5902 BstYI r'GATC_y Cuts at: 1972 3232 3599 3984 4001 5453 5539 5551 5897 Size: 1260 367 385 17 1452 86 12 346 Cuts at: 5897 1972 Size: 1977 Fragments arranged by size: 1977 1452 1260 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2330 2330 Size: 5902 BtgI C'CryG_G Cuts at: 513 1065 2118 513 Size: 552 1053 4297 Fragments arranged by size: 4297 1053 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5163 Fragments arranged by size: 5163 683 56 BtsI GCAGTG_nn' Cuts at: 2170 2204 3418 4212 4232 2170 Size: 34 1214 794 20 3840 Fragments arranged by size: 3840 1214 794 34 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5902 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5867 DraI TTT'AAA Cuts at: 3206 3206 Size: 5902 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4909 809 Size: 507 3593 1802 Fragments arranged by size: 3593 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2121 2677 3349 Size: 54 1425 50 275 18 291 556 672 Cuts at: 3349 4238 5867 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 672 556 291 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3349 1537 Size: 275 1537 4090 Fragments arranged by size: 4090 1537 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3832 1353 Size: 2479 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2103 4489 4863 5009 387 Size: 1064 138 514 2386 374 146 1280 Fragments arranged by size: 2386 1280 1064 514 374 146 138 EcoICRI GAG'CTC Cuts at: 2044 2044 Size: 5902 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2364 2498 3088 3965 4562 5349 Size: 393 470 262 134 590 877 597 787 Cuts at: 5349 5617 1239 Size: 268 1524 Fragments arranged by size: 1524 877 787 597 590 470 393 268 262 134 EcoO109I rG'GnC_Cy Cuts at: 2054 2143 2745 3077 3112 3170 2054 Size: 89 602 332 35 58 4786 Fragments arranged by size: 4786 602 332 89 58 35 EcoRI G'AATT_C Cuts at: 3211 3589 3211 Size: 378 5524 FspI TGC'GCA Cuts at: 4408 4408 Size: 5902 HaeII r_GCGC'y Cuts at: 1678 2081 5049 1678 Size: 403 2968 2531 Fragments arranged by size: 2968 2531 403 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2323 2339 2355 2371 3018 Size: 32 135 32 751 16 16 16 647 Cuts at: 3018 3050 4543 4575 4617 4649 1373 Size: 32 1493 32 42 32 2626 Fragments arranged by size: 2626 1493 751 647 135 42 32 32 32 32 32 16 16 16 HincII GTy'rAC Cuts at: 669 1657 1938 2458 3246 3492 669 Size: 988 281 520 788 246 3079 Fragments arranged by size: 3079 988 788 520 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5902 HpaI GTT'AAC Cuts at: 3492 3492 Size: 5902 KpnI G_GTAC'C Cuts at: 3227 3227 Size: 5902 MfeI C'AATT_G Cuts at: 3501 3501 Size: 5902 MluI A'CGCG_T Cuts at: 5778 5778 Size: 5902 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5016 5200 1305 Size: 3711 184 2007 Fragments arranged by size: 3711 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4080 Fragments arranged by size: 4080 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2187 2274 3125 3860 4219 4378 518 Size: 648 1021 87 851 735 359 159 2042 Fragments arranged by size: 2042 1021 851 735 648 359 159 87 MspA1I CmG'CkG Cuts at: 1606 2833 3108 3318 3995 5143 5388 1606 Size: 1227 275 210 677 1148 245 2120 Fragments arranged by size: 2120 1227 1148 677 275 245 210 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5350 NdeI CA'TA_TG Cuts at: 387 3157 387 Size: 2770 3132 NheI G'CTAG_C Cuts at: 1052 2602 1052 Size: 1550 4352 NotI GC'GGCC_GC Cuts at: 3349 3349 Size: 5902 NspI r_CATG'y Cuts at: 1132 1229 2873 3260 4805 1132 Size: 97 1644 387 1545 2229 Fragments arranged by size: 2229 1644 1545 387 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5902 PciI A'CATG_T Cuts at: 2869 4801 2869 Size: 1932 3970 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5902 PfoI T'CCnGG_A Cuts at: 2341 2341 Size: 5902 PmeI GTTT'AAAC Cuts at: 3206 3206 Size: 5902 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3971 4003 5484 5516 3971 Size: 32 1481 32 4357 Fragments arranged by size: 4357 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2054 2745 3077 3112 2054 Size: 691 332 35 4844 Fragments arranged by size: 4844 691 332 35 PshAI GACnn'nnGTC Cuts at: 1613 2223 1613 Size: 610 5292 PsiI TTA'TAA Cuts at: 3472 3472 Size: 5902 PstI C_TGCA'G Cuts at: 830 3254 830 Size: 2424 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5902 PvuII CAG'CTG Cuts at: 3108 3108 Size: 5902 SacI G_AGCT'C Cuts at: 2046 2046 Size: 5902 SalI G'TCGA_C Cuts at: 1936 3244 1936 Size: 1308 4594 SbfI CC_TGCA'GG Cuts at: 3254 3254 Size: 5902 ScaI AGT'ACT Cuts at: 4150 5862 4150 Size: 1712 4190 SfcI C'TryA_G Cuts at: 826 1045 2365 2544 3093 3250 4385 5066 5257 Size: 219 1320 179 549 157 1135 681 191 Cuts at: 5257 826 Size: 1471 Fragments arranged by size: 1471 1320 1135 681 549 219 191 179 157 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5902 SmaI CCC'GGG Cuts at: 1872 3229 1872 Size: 1357 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4006 4907 5169 820 Size: 197 926 2063 901 262 1553 Fragments arranged by size: 2063 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3199 3693 493 Size: 2706 494 2702 Fragments arranged by size: 2706 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5902 SphI G_CATG'C Cuts at: 1132 3260 1132 Size: 2128 3774 SspI AAT'ATT Cuts at: 5 52 3826 5 Size: 47 3774 2081 Fragments arranged by size: 3774 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5902 StyI C'CwwG_G Cuts at: 513 1065 3341 513 Size: 552 2276 3074 Fragments arranged by size: 3074 2276 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2819 3929 3946 4099 4284 4729 325 Size: 1255 1239 1110 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1239 1110 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2718 2815 2963 Size: 274 80 33 51 1443 741 97 148 Cuts at: 2963 4148 5860 96 Size: 1185 1712 138 Fragments arranged by size: 1712 1443 1185 741 274 148 138 97 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2879 2911 5862 5894 2879 Size: 32 2951 32 2887 Fragments arranged by size: 2951 2887 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5902 XbaI T'CTAG_A Cuts at: 3238 3238 Size: 5902 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2029 2143 2563 2837 2029 Size: 114 420 274 5094 Fragments arranged by size: 5094 420 274 114 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5902 XmaI C'CCGG_G Cuts at: 1870 3227 1870 Size: 1357 4545 XmnI GAAnn'nnTTC Cuts at: 4031 4031 Size: 5902 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3710 276 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI