(Circular) (Six-base) MAPSORT of: pFN21ASDA0252.seq Check: 7921 from: 1 to: 6739 pFN21ASDA0252 6739 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2195 4549 278 Size: 53 83 186 936 659 2354 2468 Fragments arranged by size: 2468 2354 936 659 186 83 53 AccI GT'mk_AC Cuts at: 1937 2835 4082 1937 Size: 898 1247 4594 Fragments arranged by size: 4594 1247 898 Acc65I G'GTAC_C Cuts at: 4060 4060 Size: 6739 AclI AA'CG_TT Cuts at: 1563 4866 5239 1563 Size: 3303 373 3063 Fragments arranged by size: 3303 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2991 3473 4802 6186 6454 1632 Size: 1359 482 1329 1384 268 1917 Fragments arranged by size: 1917 1384 1359 1329 482 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6542 AflIII A'CryG_T Cuts at: 1203 3205 5638 6615 1203 Size: 2002 2433 977 1327 Fragments arranged by size: 2433 2002 1327 977 AgeI A'CCGG_T Cuts at: 5570 5570 Size: 6739 AhdI GACnn_n'nnGTC Cuts at: 5468 5468 Size: 6739 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6739 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3314 1184 Size: 2130 4609 AlwNI CAG_nnn'CTG Cuts at: 2351 2375 3451 4482 6054 2351 Size: 24 1076 1031 1572 3036 Fragments arranged by size: 3036 1572 1076 1031 24 ApaI G_GGCC'C Cuts at: 2485 2485 Size: 6739 ApaLI G'TGCA_C Cuts at: 4796 5952 4796 Size: 1156 5583 ApoI r'AATT_y Cuts at: 1493 3239 3359 3841 4048 4274 4426 6535 1493 Size: 1746 120 482 207 226 152 2109 1697 Fragments arranged by size: 2109 1746 1697 482 226 207 152 120 AseI AT'TA_AT Cuts at: 160 1032 5293 160 Size: 872 4261 1606 Fragments arranged by size: 4261 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6739 AvaI C'yCGr_G Cuts at: 1870 1943 4064 1870 Size: 73 2121 4545 Fragments arranged by size: 4545 2121 73 BamHI G'GATC_C Cuts at: 3676 4069 4436 3676 Size: 393 367 5979 Fragments arranged by size: 5979 393 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3158 4060 5515 618 Size: 325 217 627 1371 902 1455 1842 Fragments arranged by size: 1842 1455 1371 902 627 325 217 BanII G_rGCy'C Cuts at: 1391 2106 2345 2485 2799 3982 4058 1391 Size: 715 239 140 314 1183 76 4072 Fragments arranged by size: 4072 1183 715 314 239 140 76 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2460 2481 3446 4011 928 Size: 971 561 21 965 565 3656 Fragments arranged by size: 3656 971 965 565 561 21 BbvCI CC'TCA_GC Cuts at: 3471 3471 Size: 6739 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4930 4964 1903 Size: 34 2993 34 3678 Fragments arranged by size: 3678 2993 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4631 5841 4631 Size: 1210 5529 BclI T'GATC_A Cuts at: 1548 3367 1548 Size: 1819 4920 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3641 3752 5350 Size: 107 122 71 631 605 1969 111 1598 Cuts at: 5350 136 Size: 1525 Fragments arranged by size: 1969 1598 1525 631 605 122 111 107 71 BglII A'GATC_T Cuts at: 3871 3989 6734 3871 Size: 118 2745 3876 Fragments arranged by size: 3876 2745 118 BlpI GC'TnA_GC Cuts at: 2344 2793 4156 2344 Size: 449 1363 4927 Fragments arranged by size: 4927 1363 449 Bme1580I G_kGCm'C Cuts at: 1790 2485 4800 5956 1790 Size: 695 2315 1156 2573 Fragments arranged by size: 2573 2315 1156 695 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6739 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4470 5428 442 Size: 416 53 83 399 24 3053 958 1753 Fragments arranged by size: 3053 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6739 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2474 2854 3244 2474 Size: 380 390 5969 Fragments arranged by size: 5969 390 380 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3548 3801 5399 1239 Size: 2309 253 1598 2579 Fragments arranged by size: 2579 2309 1598 253 Bpu10I CC'TnA_GC Cuts at: 3471 3471 Size: 6739 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2724 4864 5729 6027 2724 Size: 2140 865 298 3436 Fragments arranged by size: 3436 2140 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3720 5402 882 Size: 617 2221 1682 2219 Fragments arranged by size: 2221 2219 1682 617 BsaAI yAC'GTr Cuts at: 493 1204 4036 4530 493 Size: 711 2832 494 2702 Fragments arranged by size: 2832 2702 711 494 BsaBI GATnn'nnATC Cuts at: 3071 6733 3071 Size: 3662 3077 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2192 4546 4928 5563 Size: 53 83 186 936 659 2354 382 635 Cuts at: 5563 275 Size: 1451 Fragments arranged by size: 2354 1451 936 659 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5172 5570 5844 5991 6402 5172 Size: 398 274 147 411 5509 Fragments arranged by size: 5509 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2093 2123 1178 Size: 30 165 30 690 30 5794 Fragments arranged by size: 5794 690 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2156 2296 2380 2651 2765 2867 2939 1186 Size: 970 140 84 271 114 102 72 4986 Fragments arranged by size: 4986 970 271 140 114 102 84 72 BseYI C'CCAG_C Cuts at: 3730 3756 3920 5942 6438 3730 Size: 26 164 2022 496 4031 Fragments arranged by size: 4031 2022 496 164 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6166 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4189 4950 5978 1540 Size: 275 186 2188 761 1028 2301 Fragments arranged by size: 2301 2188 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2106 2345 2799 4800 4885 5956 2106 Size: 239 454 2001 85 1071 2889 Fragments arranged by size: 2889 2001 1071 454 239 85 BsmI GAATG_Cn' Cuts at: 3769 4250 4343 3769 Size: 481 93 6165 Fragments arranged by size: 6165 481 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2183 2183 Size: 6739 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2345 2485 2799 3982 4058 4800 Size: 399 316 239 140 314 1183 76 742 Cuts at: 4800 4885 5956 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1183 1071 742 399 316 314 239 140 85 76 BspEI T'CCGG_A Cuts at: 6402 6402 Size: 6739 BspHI T'CATG_A Cuts at: 4628 6358 4628 Size: 1730 5009 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4094 844 Size: 3250 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2892 4626 1394 Size: 429 1069 1734 3507 Fragments arranged by size: 3507 1734 1069 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3202 5234 5408 65 Size: 1183 1954 2032 174 1396 Fragments arranged by size: 2032 1954 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5383 5570 1700 Size: 110 3573 187 2869 Fragments arranged by size: 3573 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6739 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6739 BssSI C'ACGA_G Cuts at: 4799 5811 4799 Size: 1012 5727 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6739 BstBI TT'CG_AA Cuts at: 2007 5543 2007 Size: 3536 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6739 BstXI CCAn_nnnn'nTGG Cuts at: 3155 3155 Size: 6739 BstYI r'GATC_y Cuts at: 1972 2937 3676 3871 3989 4069 4436 4821 4838 Size: 965 739 195 118 80 367 385 17 Cuts at: 4838 6290 6376 6388 6734 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 965 739 385 367 346 195 118 86 80 17 12 Bsu36I CC'TnA_GG Cuts at: 2308 2352 2308 Size: 44 6695 BtgI C'CryG_G Cuts at: 513 1065 2022 2745 3465 513 Size: 552 957 723 720 3787 Fragments arranged by size: 3787 957 723 720 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3290 487 Size: 683 56 2064 3936 Fragments arranged by size: 3936 2064 683 56 BtsI GCAGTG_nn' Cuts at: 2137 3701 4255 5049 5069 2137 Size: 1564 554 794 20 3807 Fragments arranged by size: 3807 1564 794 554 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6739 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6704 DraI TTT'AAA Cuts at: 4043 4043 Size: 6739 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5746 809 Size: 507 4430 1802 Fragments arranged by size: 4430 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4186 5075 6704 Size: 54 1425 50 275 18 2356 889 1629 Cuts at: 6704 8 Size: 43 Fragments arranged by size: 2356 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4186 1537 Size: 275 2374 4090 Fragments arranged by size: 4090 2374 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2398 2419 2424 2537 2628 2844 2853 2916 Size: 1045 21 5 113 91 216 9 63 Cuts at: 2916 2928 3375 4669 1353 Size: 12 447 1294 3423 Fragments arranged by size: 3423 1294 1045 447 216 113 91 63 21 12 9 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3951 5326 5700 5846 387 Size: 1064 138 2362 1375 374 146 1280 Fragments arranged by size: 2362 1375 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2104 2343 2797 2104 Size: 239 454 6046 Fragments arranged by size: 6046 454 239 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2991 3473 3548 3801 4802 5399 6186 Size: 393 1359 482 75 253 1001 597 787 Cuts at: 6186 6454 1239 Size: 268 1524 Fragments arranged by size: 1524 1359 1001 787 597 482 393 268 253 75 EcoNI CCTnn'n_nnAGG Cuts at: 2096 2355 2096 Size: 259 6480 EcoO109I rG'GnC_Cy Cuts at: 2481 3475 3544 3634 2481 Size: 994 69 90 5586 Fragments arranged by size: 5586 994 90 69 EcoRI G'AATT_C Cuts at: 3239 3841 4048 4426 3239 Size: 602 207 378 5552 Fragments arranged by size: 5552 602 378 207 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3365 3925 3365 Size: 560 6179 FspI TGC'GCA Cuts at: 5245 5245 Size: 6739 HaeII r_GCGC'y Cuts at: 1678 5886 1678 Size: 4208 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2093 2125 2396 2402 2428 Size: 32 135 32 521 32 271 6 26 Cuts at: 2428 2434 2556 2588 2720 2752 2855 2879 2887 Size: 6 122 32 132 32 103 24 8 Cuts at: 2887 2911 3802 3834 5380 5412 5454 5486 1373 Size: 24 891 32 1546 32 42 32 2626 Fragments arranged by size: 2626 1546 891 521 271 135 132 122 103 42 32 32 32 32 32 32 32 32 26 24 24 8 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 4083 4329 669 Size: 988 281 2145 246 3079 Fragments arranged by size: 3079 2145 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6739 HpaI GTT'AAC Cuts at: 4329 4329 Size: 6739 KpnI G_GTAC'C Cuts at: 4064 4064 Size: 6739 MfeI C'AATT_G Cuts at: 4338 4338 Size: 6739 MluI A'CGCG_T Cuts at: 3205 6615 3205 Size: 3410 3329 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5853 6037 1305 Size: 4548 184 2007 Fragments arranged by size: 4548 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4917 Fragments arranged by size: 4917 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3195 4697 5056 5215 518 Size: 648 2029 1502 359 159 2042 Fragments arranged by size: 2042 2029 1502 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2076 2336 2792 3559 3598 3734 3760 4155 Size: 470 260 456 767 39 136 26 395 Cuts at: 4155 4832 5980 6225 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 767 677 470 456 395 260 245 136 39 26 NcoI C'CATG_G Cuts at: 513 1065 2022 2745 3465 513 Size: 552 957 723 720 3787 Fragments arranged by size: 3787 957 723 720 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6739 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6739 NotI GC'GGCC_GC Cuts at: 4186 4186 Size: 6739 NspI r_CATG'y Cuts at: 1132 1229 3028 3295 4097 5642 1132 Size: 97 1799 267 802 1545 2229 Fragments arranged by size: 2229 1799 1545 802 267 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6739 PciI A'CATG_T Cuts at: 5638 5638 Size: 6739 PflMI CCAn_nnn'nTGG Cuts at: 1108 3673 1108 Size: 2565 4174 PmeI GTTT'AAAC Cuts at: 4043 4043 Size: 6739 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4808 4840 6321 6353 4808 Size: 32 1481 32 5194 Fragments arranged by size: 5194 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3475 3475 Size: 6739 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6739 PsiI TTA'TAA Cuts at: 3357 4309 3357 Size: 952 5787 PspOMI G'GGCC_C Cuts at: 2481 2481 Size: 6739 PstI C_TGCA'G Cuts at: 830 2173 4091 830 Size: 1343 1918 3478 Fragments arranged by size: 3478 1918 1343 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6739 PvuII CAG'CTG Cuts at: 2336 2792 3734 3760 2336 Size: 456 942 26 5315 Fragments arranged by size: 5315 942 456 26 SacI G_AGCT'C Cuts at: 2106 2345 2799 2106 Size: 239 454 6046 Fragments arranged by size: 6046 454 239 SalI G'TCGA_C Cuts at: 1936 4081 1936 Size: 2145 4594 SbfI CC_TGCA'GG Cuts at: 4091 4091 Size: 6739 ScaI AGT'ACT Cuts at: 4987 6699 4987 Size: 1712 5027 SfcI C'TryA_G Cuts at: 826 1045 2169 4087 5222 5903 6094 826 Size: 219 1124 1918 1135 681 191 1471 Fragments arranged by size: 1918 1471 1135 1124 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6739 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6739 SmaI CCC'GGG Cuts at: 1872 4066 1872 Size: 2194 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2739 4843 5744 6006 820 Size: 197 926 796 2104 901 262 1553 Fragments arranged by size: 2104 1553 926 901 796 262 197 SnaBI TAC'GTA Cuts at: 493 4036 4530 493 Size: 3543 494 2702 Fragments arranged by size: 3543 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6739 SphI G_CATG'C Cuts at: 1132 3295 4097 1132 Size: 2163 802 3774 Fragments arranged by size: 3774 2163 802 SspI AAT'ATT Cuts at: 5 52 4663 5 Size: 47 4611 2081 Fragments arranged by size: 4611 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6739 StyI C'CwwG_G Cuts at: 513 1065 2022 2745 3465 3954 3969 3983 4178 Size: 552 957 723 720 489 15 14 195 Cuts at: 4178 513 Size: 3074 Fragments arranged by size: 3074 957 723 720 552 489 195 15 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3436 4766 4783 4936 5121 5566 325 Size: 1255 1856 1330 17 153 185 445 1498 Fragments arranged by size: 1856 1498 1330 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3883 4985 6697 Size: 274 80 33 51 1443 1906 1102 1712 Cuts at: 6697 96 Size: 138 Fragments arranged by size: 1906 1712 1443 1102 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3045 3077 6699 6731 3045 Size: 32 3622 32 3053 Fragments arranged by size: 3622 3053 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6739 XbaI T'CTAG_A Cuts at: 3307 4075 3307 Size: 768 5971 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2444 2444 Size: 6739 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6739 XmaI C'CCGG_G Cuts at: 1870 4064 1870 Size: 2194 4545 XmnI GAAnn'nnTTC Cuts at: 4868 4868 Size: 6739 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2193 4547 276 Size: 53 83 186 936 659 2354 2468 Fragments arranged by size: 2468 2354 936 659 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI