Restriction map for
pFN21ASDA0245
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII Alw44I AocI ApaI
ApaLI AscI Asp700I Asp718I AspI
AviII AvrII BamHI BbeI BbsI
BbvCI BciVI BclI BfrI BlnI
BpuAI BsaBI BseAI BseRI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI EagI
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FauI FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI KspI MamI MluI
MroI MvnI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PmeI PshAI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI TfiI
Tth111I TthHB8I Van91I XhoI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AcsI 795 AAATTT [GA]^AATT[CT]
AcyI 670 GACGCC G[GA]^CG[CT]C
AfaI 214 GTAC GT^AC
AgeI 412 ACCGGT A^CCGGT
AhdI 1060 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1185 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 603 AGCGCT AGC^GCT
ApoI 795 AAATTT [GA]^AATT[CT]
AseI 1345 ATTAAT AT^TAAT
AsnI 1345 ATTAAT AT^TAAT
AspEI 1060 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 658 GAGCAC G[AT]GC[AT]^C
AvaI 1040 CCCGGG C^[CT]CG[GA]G
BalI 806 TGGCCA TGG^CCA
BbrPI 566 CACGTG CAC^GTG
BcgI 167 GCAACATGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 131 AGATCT A^GATCT
BlpI 1269 GCTGAGC GC^T[ACGT]AGC
BpmI 1379 CTGGAG CTGGAG(16/14)
Bpu1102I 1269 GCTGAGC GC^T[ACGT]AGC
BsaAI 566 CACGTG [CT]AC^GT[GA]
BsaHI 670 GACGCC G[GA]^CG[CT]C
BsaI 387 GGTCTC GGTCTC(1/5)
BsgI 571 GTGCAG GTGCAG(16/14)
BsiHKAI 658 GAGCAC G[AT]GC[AT]^C
BsmBI 499 CGTCTC CGTCTC(1/5)
BsoBI 1040 CCCGGG C^[CT]CG[GA]G
BspMI 1409 ACCTGC ACCTGC(4/8)
BsrFI 412 ACCGGT [GA]^CCGG[CT]
BstXI 930 CCAGACGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1269 GCTGAGC GC^T[ACGT]AGC
CfoI 604 GCGC GCG^C
Cfr10I 412 ACCGGT [GA]^CCGG[CT]
DsaI 1124 CCATGG C^C[GA][CT]GG
EaeI 806 TGGCCA [CT]^GGCC[GA]
Eam1105I 1060 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 603 AGCGCT AGC^GCT
EcoT22I 998 ATGCAT ATGCA^T
HaeII 603 AGCGCT [GA]GCGC^[CT]
HhaI 604 GCGC GCG^C
Hin1I 670 GACGCC G[GA]^CG[CT]C
HinP1I 604 GCGC G^CGC
MluNI 806 TGGCCA TGG^CCA
MscI 806 TGGCCA TGG^CCA
NcoI 1124 CCATGG C^CATGG
NsiI 998 ATGCAT ATGCA^T
NspI 941 GCATGT [GA]CATG^[CT]
PinAI 412 ACCGGT A^CCGGT
PmaCI 566 CACGTG CAC^GTG
PmlI 566 CACGTG CAC^GTG
PpuMI 100 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 1345 ATTAAT AT^TAAT
RsaI 214 GTAC GT^AC
SapI 264 GCTCTTC GCTCTTC(1/4)
SmaI 1040 CCCGGG CCC^GGG
SmlI 1264 CTCAAG C^T[CT][GA]AG
StuI 350 AGGCCT AGG^CCT
XbaI 345 TCTAGA T^CTAGA
XmaCI 1040 CCCGGG C^CCGGG
XmaI 1040 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BanI 280 GGTGCC G^G[CT][GA]CC
1468 GGCACC
BfaI 346 CTAG C^TAG
397 CTAG
BglI 15 GCCTGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1245 GCCCAAGCGGC
BsaWI 412 ACCGGT [AT]^CCGG[AT]
1056 ACCGGA
BsrI 1190 ACTGG ACTGG(1/-1)
60 CCAGT
BstYI 131 AGATCT [GA]^GATC[CT]
185 GGATCT
DraII 100 AGGTCCT [GA]G^G[ACGT]CC[CT]
989 GGGGCCT
EcoO109I 100 AGGTCCT [GA]G^G[ACGT]CC[CT]
989 GGGGCCT
HgaI 670 GACGC GACGC(5/10)
834 GACGC
HinfI 1382 GAGTC G^A[ACGT]TC
1417 GAGTC
MaeI 346 CTAG C^TAG
397 CTAG
MfeI 827 CAATTG C^AATTG
1116 CAATTG
MflI 131 AGATCT [GA]^GATC[CT]
185 GGATCT
MslI 564 CACACGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
618 CATTGTCATG
MunI 827 CAATTG C^AATTG
1116 CAATTG
NdeI 519 CATATG CA^TATG
1299 CATATG
PleI 1382 GAGTC GAGTC(4/5)
1417 GAGTC
XcmI 200 CCAAGGGTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
614 CCATCATTGTCATGG
XhoII 131 AGATCT [GA]^GATC[CT]
185 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AflIII AluI AlwI AvaII
BanII BbvI BcnI BmyI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PvuII Sau3AI
Sau96I ScrFI SfaNI StyI Tru9I
TseI Tsp45I Tsp509I TspRI