(Circular) (Six-base) MAPSORT of: pFN21ASDA0245.seq Check: 9534 from: 1 to: 6274 pFN21ASDA0245 6274 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4084 278 Size: 53 83 186 936 2548 2468 Fragments arranged by size: 2548 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3617 1937 Size: 1680 4594 Acc65I G'GTAC_C Cuts at: 3595 3595 Size: 6274 AclI AA'CG_TT Cuts at: 1563 4401 4774 1563 Size: 2838 373 3063 Fragments arranged by size: 3063 2838 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2165 3184 3190 4337 5721 5989 1632 Size: 533 1019 6 1147 1384 268 1917 Fragments arranged by size: 1917 1384 1147 1019 533 268 6 AfeI AGC'GCT Cuts at: 2628 2628 Size: 6274 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6077 AflIII A'CryG_T Cuts at: 1203 2588 2590 2601 5173 6150 1203 Size: 1385 2 11 2572 977 1327 Fragments arranged by size: 2572 1385 1327 977 11 2 AgeI A'CCGG_T Cuts at: 2435 5105 2435 Size: 2670 3604 AhdI GACnn_n'nnGTC Cuts at: 3088 5003 3088 Size: 1915 4359 AleI CACnn'nnGTG Cuts at: 1166 2591 1166 Size: 1425 4849 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2564 2609 1184 Size: 1380 45 4849 Fragments arranged by size: 4849 1380 45 AlwNI CAG_nnn'CTG Cuts at: 3213 4017 5589 3213 Size: 804 1572 3898 Fragments arranged by size: 3898 1572 804 ApaLI G'TGCA_C Cuts at: 4331 5487 4331 Size: 1156 5118 ApoI r'AATT_y Cuts at: 1493 2818 3583 3809 3961 6070 1493 Size: 1325 765 226 152 2109 1697 Fragments arranged by size: 2109 1697 1325 765 226 152 AseI AT'TA_AT Cuts at: 160 1032 3369 4828 160 Size: 872 2337 1459 1606 Fragments arranged by size: 2337 1606 1459 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6274 AvaI C'yCGr_G Cuts at: 1870 1943 3063 3599 1870 Size: 73 1120 536 4545 Fragments arranged by size: 4545 1120 536 73 BamHI G'GATC_C Cuts at: 3604 3971 3604 Size: 367 5907 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2303 3491 3595 5050 618 Size: 325 217 627 516 1188 104 1455 1842 Fragments arranged by size: 1842 1455 1188 627 516 325 217 104 BanII G_rGCy'C Cuts at: 1391 2289 2433 3062 3270 3593 1391 Size: 898 144 629 208 323 4072 Fragments arranged by size: 4072 898 629 323 208 144 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5303 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2179 2213 4465 4499 1903 Size: 34 242 34 2252 34 3678 Fragments arranged by size: 3678 2252 242 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4166 5376 4166 Size: 1210 5064 BclI T'GATC_A Cuts at: 1548 1548 Size: 6274 BfrBI ATG'CAT Cuts at: 3023 3023 Size: 6274 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2044 3274 4885 Size: 107 122 71 631 605 372 1230 1611 Cuts at: 4885 136 Size: 1525 Fragments arranged by size: 1611 1525 1230 631 605 372 122 107 71 BglII A'GATC_T Cuts at: 2154 6269 2154 Size: 4115 2159 BlpI GC'TnA_GC Cuts at: 3293 3691 3293 Size: 398 5876 Bme1580I G_kGCm'C Cuts at: 1790 2308 2588 3351 4335 5491 1790 Size: 518 280 763 984 1156 2573 Fragments arranged by size: 2573 1156 984 763 518 280 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6274 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4005 4963 442 Size: 416 53 83 399 24 2588 958 1753 Fragments arranged by size: 2588 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6274 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2725 2725 Size: 6274 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3423 4934 1239 Size: 2184 1511 2579 Fragments arranged by size: 2579 2184 1511 Bpu10I CC'TnA_GC Cuts at: 2203 2918 2203 Size: 715 5559 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3272 4399 5264 5562 3272 Size: 1127 865 298 3984 Fragments arranged by size: 3984 1127 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2416 4937 882 Size: 617 917 2521 2219 Fragments arranged by size: 2521 2219 917 617 BsaAI yAC'GTr Cuts at: 493 1204 2591 3571 4065 493 Size: 711 1387 980 494 2702 Fragments arranged by size: 2702 1387 980 711 494 BsaBI GATnn'nnATC Cuts at: 6268 6268 Size: 6274 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2694 4081 4463 5098 Size: 53 83 186 936 1161 1387 382 635 Cuts at: 5098 275 Size: 1451 Fragments arranged by size: 1451 1387 1161 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2435 3079 4707 5105 5379 5526 5937 2435 Size: 644 1628 398 274 147 411 2772 Fragments arranged by size: 2772 1628 644 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2231 2261 1178 Size: 30 165 30 828 30 5191 Fragments arranged by size: 5191 828 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6274 BseYI C'CCAG_C Cuts at: 2323 2492 3495 5477 5973 2323 Size: 169 1003 1982 496 2624 Fragments arranged by size: 2624 1982 1003 496 169 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2615 1163 Size: 573 879 4822 Fragments arranged by size: 4822 879 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3724 4485 5513 1540 Size: 275 186 1723 761 1028 2301 Fragments arranged by size: 2301 1723 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2685 4335 4420 5491 2685 Size: 1650 85 1071 3468 Fragments arranged by size: 3468 1650 1071 85 BsmI GAATG_Cn' Cuts at: 3785 3878 3785 Size: 93 6181 BsmBI CGTCTCn'nnnn_ Cuts at: 2528 2528 Size: 6274 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2289 2308 2433 2588 2685 3062 3270 Size: 399 499 19 125 155 97 377 208 Cuts at: 3270 3351 3593 4335 4420 5491 1391 Size: 81 242 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 499 399 377 242 208 155 125 97 85 81 19 BspEI T'CCGG_A Cuts at: 5937 5937 Size: 6274 BspHI T'CATG_A Cuts at: 4163 5893 4163 Size: 1730 4544 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3441 3629 844 Size: 2597 188 3489 Fragments arranged by size: 3489 2597 188 BsrBI CCG'CTC Cuts at: 1394 1823 4161 1394 Size: 429 2338 3507 Fragments arranged by size: 3507 2338 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2388 2631 2985 4769 4943 65 Size: 1183 1140 243 354 1784 174 1396 Fragments arranged by size: 1784 1396 1183 1140 354 243 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2435 4918 5105 1700 Size: 110 625 2483 187 2869 Fragments arranged by size: 2869 2483 625 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6274 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6274 BssSI C'ACGA_G Cuts at: 4334 5346 4334 Size: 1012 5262 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6274 BstBI TT'CG_AA Cuts at: 2007 5078 2007 Size: 3071 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6274 BstXI CCAn_nnnn'nTGG Cuts at: 2960 2960 Size: 6274 BstYI r'GATC_y Cuts at: 1972 2154 2208 3604 3971 4356 4373 5825 5911 Size: 182 54 1396 367 385 17 1452 86 Cuts at: 5911 5923 6269 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1396 385 367 346 182 86 54 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 3147 513 Size: 552 957 1125 3640 Fragments arranged by size: 3640 1125 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2434 487 Size: 683 56 1208 4327 Fragments arranged by size: 4327 1208 683 56 BtsI GCAGTG_nn' Cuts at: 2241 2414 2929 3790 4584 4604 2241 Size: 173 515 861 794 20 3911 Fragments arranged by size: 3911 861 794 515 173 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6274 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6239 DraI TTT'AAA Cuts at: 3578 3578 Size: 6274 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5281 809 Size: 507 3965 1802 Fragments arranged by size: 3965 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2829 3721 4610 Size: 54 1425 50 275 18 999 892 889 Cuts at: 4610 6239 8 Size: 1629 43 Fragments arranged by size: 1629 1425 999 892 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3721 1537 Size: 275 1909 4090 Fragments arranged by size: 4090 1909 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2294 2759 3359 4204 1353 Size: 941 465 600 845 3423 Fragments arranged by size: 3423 941 845 600 465 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2390 4861 5235 5381 387 Size: 1064 138 801 2471 374 146 1280 Fragments arranged by size: 2471 1280 1064 801 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2165 3184 3190 3423 4337 4934 5721 Size: 393 533 1019 6 233 914 597 787 Cuts at: 5721 5989 1239 Size: 268 1524 Fragments arranged by size: 1524 1019 914 787 597 533 393 268 233 6 EcoO109I rG'GnC_Cy Cuts at: 2124 3013 2124 Size: 889 5385 EcoRI G'AATT_C Cuts at: 3583 3961 3583 Size: 378 5896 FspI TGC'GCA Cuts at: 4780 4780 Size: 6274 HaeII r_GCGC'y Cuts at: 1678 2630 5421 1678 Size: 952 2791 2531 Fragments arranged by size: 2791 2531 952 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2093 2125 3074 3106 4915 Size: 32 135 32 521 32 949 32 1809 Cuts at: 4915 4947 4989 5021 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1809 949 521 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3618 3864 669 Size: 988 281 1680 246 3079 Fragments arranged by size: 3079 1680 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6274 HpaI GTT'AAC Cuts at: 3864 3864 Size: 6274 KpnI G_GTAC'C Cuts at: 3599 3599 Size: 6274 MfeI C'AATT_G Cuts at: 2850 3139 3873 2850 Size: 289 734 5251 Fragments arranged by size: 5251 734 289 MluI A'CGCG_T Cuts at: 6150 6150 Size: 6274 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3220 5388 5572 1305 Size: 1915 2168 184 2007 Fragments arranged by size: 2168 2007 1915 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2831 10 Size: 54 1425 343 999 3453 Fragments arranged by size: 3453 1425 999 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2591 2645 4232 4591 4750 518 Size: 648 1425 54 1587 359 159 2042 Fragments arranged by size: 2042 1587 1425 648 359 159 54 MspA1I CmG'CkG Cuts at: 1606 2143 2496 2973 3258 3690 4367 5515 5760 Size: 537 353 477 285 432 677 1148 245 Cuts at: 5760 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 537 477 432 353 285 245 NcoI C'CATG_G Cuts at: 513 1065 2022 3147 513 Size: 552 957 1125 3640 Fragments arranged by size: 3640 1125 957 552 NdeI CA'TA_TG Cuts at: 387 2543 3323 387 Size: 2156 780 3338 Fragments arranged by size: 3338 2156 780 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6274 NotI GC'GGCC_GC Cuts at: 3721 3721 Size: 6274 NsiI A_TGCA'T Cuts at: 3025 3025 Size: 6274 NspI r_CATG'y Cuts at: 1132 1229 2968 3632 5177 1132 Size: 97 1739 664 1545 2229 Fragments arranged by size: 2229 1739 1545 664 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6274 PciI A'CATG_T Cuts at: 5173 5173 Size: 6274 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6274 PfoI T'CCnGG_A Cuts at: 3443 3443 Size: 6274 PmeI GTTT'AAAC Cuts at: 3578 3578 Size: 6274 PmlI CAC'GTG Cuts at: 2591 2591 Size: 6274 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4343 4375 5856 5888 4343 Size: 32 1481 32 4729 Fragments arranged by size: 4729 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2124 2124 Size: 6274 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6274 PsiI TTA'TAA Cuts at: 3844 3844 Size: 6274 PstI C_TGCA'G Cuts at: 830 3626 830 Size: 2796 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6274 PvuII CAG'CTG Cuts at: 2143 2496 2973 2143 Size: 353 477 5444 Fragments arranged by size: 5444 477 353 SalI G'TCGA_C Cuts at: 1936 3616 1936 Size: 1680 4594 SapI GCTCTTCn'nnn_ Cuts at: 2294 2294 Size: 6274 SbfI CC_TGCA'GG Cuts at: 3626 3626 Size: 6274 ScaI AGT'ACT Cuts at: 4522 6234 4522 Size: 1712 4562 SfcI C'TryA_G Cuts at: 826 1045 3622 4757 5438 5629 826 Size: 219 2577 1135 681 191 1471 Fragments arranged by size: 2577 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6274 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6274 SmaI CCC'GGG Cuts at: 1872 3065 3601 1872 Size: 1193 536 4545 Fragments arranged by size: 4545 1193 536 SmlI C'TyrA_G Cuts at: 820 1017 1943 3287 4378 5279 5541 820 Size: 197 926 1344 1091 901 262 1553 Fragments arranged by size: 1553 1344 1091 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3571 4065 493 Size: 3078 494 2702 Fragments arranged by size: 3078 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6274 SphI G_CATG'C Cuts at: 1132 3632 1132 Size: 2500 3774 SspI AAT'ATT Cuts at: 5 52 4198 5 Size: 47 4146 2081 Fragments arranged by size: 4146 2081 47 StuI AGG'CCT Cuts at: 1517 2375 1517 Size: 858 5416 StyI C'CwwG_G Cuts at: 513 1065 2022 2223 2616 2826 3147 3713 513 Size: 552 957 201 393 210 321 566 3074 Fragments arranged by size: 3074 957 566 552 393 321 210 201 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2068 2236 2291 3413 3509 4301 4318 Size: 1255 488 168 55 1122 96 792 17 Cuts at: 4318 4471 4656 5101 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1122 792 488 445 185 168 153 96 55 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4520 6232 96 Size: 274 80 33 51 1443 2543 1712 138 Fragments arranged by size: 2543 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2231 2263 6234 6266 2231 Size: 32 3971 32 2239 Fragments arranged by size: 3971 2239 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6274 XbaI T'CTAG_A Cuts at: 2368 3610 2368 Size: 1242 5032 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2230 2644 2230 Size: 414 5860 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6274 XmaI C'CCGG_G Cuts at: 1870 3063 3599 1870 Size: 1193 536 4545 Fragments arranged by size: 4545 1193 536 XmnI GAAnn'nnTTC Cuts at: 4403 4403 Size: 6274 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4082 276 Size: 53 83 186 936 2548 2468 Fragments arranged by size: 2548 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI