Restriction map for
pFN21ASDA0239
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# NO CUT ENZYMES ( 90 enzymes) #
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Acc65I AclI AcsI AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BbeI BbrPI BciVI BlnI
BsaBI BsiEI BsiWI Bsp1407I BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FseI
HaeII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
SbfI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tsp509I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 1378 GACGTC GACGT^C
AccI 1270 GTCGAC GT^[AC][GT]AC
AccIII 158 TCCGGA T^CCGGA
AcyI 1378 GACGTC G[GA]^CG[CT]C
AflII 460 CTTAAG C^TTAAG
ApaI 1101 GGGCCC GGGCC^C
Asp700I 1398 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1175 TGCGCA TGC^GCA
BclI 170 TGATCA T^GATCA
BfrI 460 CTTAAG C^TTAAG
BglII 893 AGATCT A^GATCT
BsaAI 1106 CACGTA [CT]AC^GT[GA]
BsaHI 1378 GACGTC G[GA]^CG[CT]C
BsaWI 158 TCCGGA [AT]^CCGG[AT]
BseAI 158 TCCGGA T^CCGGA
BsmBI 559 GAGACG CGTCTC(1/5)
BsmI 946 GAATGC GAATGC(1/-1)
BspDI 1573 ATCGAT AT^CGAT
BspEI 158 TCCGGA T^CCGGA
BsrBI 1243 GAGCGG CCGCTC(-3/-3)
BstXI 2079 CCAAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 1573 ATCGAT AT^CGAT
DrdI 2068 GACTCAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 170 TGATCA T^GATCA
FspI 1175 TGCGCA TGC^GCA
Hin1I 1378 GACGTC G[GA]^CG[CT]C
HincII 1270 GTCGAC GT[CT]^[GA]AC
HindII 1270 GTCGAC GT[CT]^[GA]AC
MroI 158 TCCGGA T^CCGGA
MseI 461 TTAA T^TAA
NcoI 238 CCATGG C^CATGG
PshAI 607 GACGTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 442 GAGCTC GAGCT^C
SalI 1270 GTCGAC G^TCGAC
SapI 1401 GCTCTTC GCTCTTC(1/4)
ScaI 1286 AGTACT AGT^ACT
SexAI 1758 ACCTGGT A^CC[AT]GGT
SmaI 2321 CCCGGG CCC^GGG
SphI 685 GCATGC GCATG^C
TfiI 843 GATTC G^A[AT]TC
Tru9I 461 TTAA T^TAA
XcmI 907 CCAGCCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2321 CCCGGG C^CCGGG
XmaI 2321 CCCGGG C^CCGGG
XmnI 1398 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 608 ACGTGT A^C[GA][CT]GT
733 ACGTGT
AocI 622 CCTGAGG CC^T[ACGT]AGG
847 CCTGAGG
AspHI 442 GAGCTC G[AT]GC[AT]^C
1087 GAGCAC
BamHI 311 GGATCC G^GATCC
695 GGATCC
BbsI 1878 GAAGAC GAAGAC(2/6)
649 GTCTTC
BbvCI 1231 GCTGAGG CCTCAGC(-5/-2)
1258 GCTGAGG
BcgI 420 CGAGATTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1671 CGAGGACCCTGC
BglI 384 GCCTGATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1142 GCCAGGCTGGC
BpuAI 1878 GAAGAC GAAGAC(2/6)
649 GTCTTC
BsaI 523 GAGACC GGTCTC(1/5)
2035 GAGACC
BsiHKAI 442 GAGCTC G[AT]GC[AT]^C
1087 GAGCAC
BsrFI 1227 GCCGGC [GA]^CCGG[CT]
2117 GCCGGC
Bsu36I 622 CCTGAGG CC^T[ACGT]AGG
847 CCTGAGG
Cfr10I 1227 GCCGGC [GA]^CCGG[CT]
2117 GCCGGC
Eco81I 622 CCTGAGG CC^T[ACGT]AGG
847 CCTGAGG
EcoT14I 238 CCATGG C^C[AT][AT]GG
2216 CCAAGG
HgaI 561 GACGC GACGC(5/10)
986 GCGTC
NaeI 1227 GCCGGC GCC^GGC
2117 GCCGGC
NgoMIV 1227 GCCGGC G^CCGGC
2117 GCCGGC
SmlI 460 CTTAAG C^T[CT][GA]AG
700 CTCAAG
StuI 1551 AGGCCT AGG^CCT
2170 AGGCCT
StyI 238 CCATGG C^C[AT][AT]GG
2216 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoO65I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XhoII