Restriction map for
pFN21ASDA0239
(Circular) (Six-base) MAPSORT of: pFN21ASDA0239.seq Check: 9540 from: 1 to: 7099
pFN21ASDA0239 7099 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2281 2990 3546 2281
Size: 709 556 5834
Fragments arranged by size:
5834 709 556
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3405 4909 278
Size: 53 83 186 936 1869 1504 2468
Fragments arranged by size:
2468 1869 1504 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3294 4442 1937
Size: 1357 1148 4594
Fragments arranged by size:
4594 1357 1148
Acc65I G'GTAC_C
Cuts at: 4420 4420
Size: 7099
AclI AA'CG_TT
Cuts at: 1563 5226 5599 1563
Size: 3663 373 3063
Fragments arranged by size:
3663 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3341 3440 4203 5162 6546 6814 1632
Size: 1709 99 763 959 1384 268 1917
Fragments arranged by size:
1917 1709 1384 959 763 268 99
AflII C'TTAA_G
Cuts at: 820 1017 2483 820
Size: 197 1466 5436
Fragments arranged by size:
5436 1466 197
AflIII A'CryG_T
Cuts at: 1203 2631 2756 5998 6975 1203
Size: 1428 125 3242 977 1327
Fragments arranged by size:
3242 1428 1327 977 125
AgeI A'CCGG_T
Cuts at: 5930 5930
Size: 7099
AhdI GACnn_n'nnGTC
Cuts at: 5828 5828
Size: 7099
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7099
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2125 2313 2402 3836 1184
Size: 941 188 89 1434 4447
Fragments arranged by size:
4447 1434 941 188 89
AlwNI CAG_nnn'CTG
Cuts at: 2940 2961 3211 3349 3565 4085 4842 6414 2940
Size: 21 250 138 216 520 757 1572 3625
Fragments arranged by size:
3625 1572 757 520 250 216 138 21
ApaI G_GGCC'C
Cuts at: 3128 3128
Size: 7099
ApaLI G'TGCA_C
Cuts at: 5156 6312 5156
Size: 1156 5943
ApoI r'AATT_y
Cuts at: 1493 4408 4634 4786 6895 1493
Size: 2915 226 152 2109 1697
Fragments arranged by size:
2915 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5653 160
Size: 872 4621 1606
Fragments arranged by size:
4621 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7099
AvaI C'yCGr_G
Cuts at: 1870 1943 2374 3804 4344 4424 1870
Size: 73 431 1430 540 80 4545
Fragments arranged by size:
4545 1430 540 431 80 73
BamHI G'GATC_C
Cuts at: 2334 2718 4429 4796 2334
Size: 384 1711 367 4637
Fragments arranged by size:
4637 1711 384 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2293 2728 2978 3602 3687
Size: 325 217 627 506 435 250 624 85
Cuts at: 3687 4187 4420 5875 618
Size: 500 233 1455 1842
Fragments arranged by size:
1842 1455 627 624 506 500 435 325
250 233 217 85
BanII G_rGCy'C
Cuts at: 1391 2325 2402 2469 2907 2947 3128 3141 3153
Size: 934 77 67 438 40 181 13 12
Cuts at: 3153 4418 1391
Size: 1265 4072
Fragments arranged by size:
4072 1265 934 438 181 77 67 40
13 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2665 3908 928
Size: 971 766 1243 4119
Fragments arranged by size:
4119 1243 971 766
BbvCI CC'TCA_GC
Cuts at: 3255 3282 3255
Size: 27 7072
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2432 2466 3683 3717 5290 5324 1903
Size: 34 495 34 1217 34 1573 34 3678
Fragments arranged by size:
3678 1573 1217 495 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4991 6201 4991
Size: 1210 5889
BclI T'GATC_A
Cuts at: 1548 2193 1548
Size: 645 6454
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2413 3171 5710
Size: 107 122 71 631 605 741 758 2539
Cuts at: 5710 136
Size: 1525
Fragments arranged by size:
2539 1525 758 741 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2916 7094 2916
Size: 4178 2921
BlpI GC'TnA_GC
Cuts at: 2228 3264 3752 4516 2228
Size: 1036 488 764 4811
Fragments arranged by size:
4811 1036 764 488
Bme1580I G_kGCm'C
Cuts at: 1790 2733 2764 2860 3128 3690 5160 6316 1790
Size: 943 31 96 268 562 1470 1156 2573
Fragments arranged by size:
2573 1470 1156 943 562 268 96 31
BmgBI CAC'GTC
Cuts at: 1318 2632 2757 3968 1318
Size: 1314 125 1211 4449
Fragments arranged by size:
4449 1314 1211 125
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4830 5788 442
Size: 416 53 83 399 24 3413 958 1753
Fragments arranged by size:
3413 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7099
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4093 4093
Size: 7099
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2483 2555 2564 3503 3690 3867 5759 1239
Size: 1244 72 9 939 187 177 1892 2579
Fragments arranged by size:
2579 1892 1244 939 187 177 72 9
Bpu10I CC'TnA_GC
Cuts at: 3255 3282 4128 3255
Size: 27 846 6226
Fragments arranged by size:
6226 846 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2708 5224 6089 6387 2708
Size: 2516 865 298 3420
Fragments arranged by size:
3420 2516 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2540 4052 5762 882
Size: 617 1041 1512 1710 2219
Fragments arranged by size:
2219 1710 1512 1041 617
BsaAI yAC'GTr
Cuts at: 493 1204 3131 4396 4890 493
Size: 711 1927 1265 494 2702
Fragments arranged by size:
2702 1927 1265 711 494
BsaBI GATnn'nnATC
Cuts at: 7093 7093
Size: 7099
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3402 4906 5288 5923
Size: 53 83 186 936 1869 1504 382 635
Cuts at: 5923 275
Size: 1451
Fragments arranged by size:
1869 1504 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2181 5532 5930 6204 6351 6762 2181
Size: 3351 398 274 147 411 2518
Fragments arranged by size:
3351 2518 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2537 2567 1178
Size: 30 165 30 1134 30 5710
Fragments arranged by size:
5710 1134 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2552 2817 2820 3239 3755 4376 1186
Size: 1366 265 3 419 516 621 3909
Fragments arranged by size:
3909 1366 621 516 419 265 3
BseYI C'CCAG_C
Cuts at: 2123 2403 2596 2929 2940 3161 3387 4202 6302
Size: 280 193 333 11 221 226 815 2100
Cuts at: 6302 6798 2123
Size: 496 2424
Fragments arranged by size:
2424 2100 815 496 333 280 226 221
193 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2309 3656 4352 1163
Size: 573 573 1347 696 3910
Fragments arranged by size:
3910 1347 696 573 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4549 5310 6338 1540
Size: 275 186 2548 761 1028 2301
Fragments arranged by size:
2548 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2469 3114 5160 5245 6316 2469
Size: 645 2046 85 1071 3252
Fragments arranged by size:
3252 2046 1071 645 85
BsmI GAATG_Cn'
Cuts at: 2975 4610 4703 2975
Size: 1635 93 5371
Fragments arranged by size:
5371 1635 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2576 2576
Size: 7099
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2325 2402 2469 2733 2764 2860 2907
Size: 399 535 77 67 264 31 96 47
Cuts at: 2907 2947 3114 3128 3141 3153 3690 4418 5160
Size: 40 167 14 13 12 537 728 742
Cuts at: 5160 5245 6316 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 728 537 535 399 264
167 96 85 77 67 47 40 31
14 13 12
BspEI T'CCGG_A
Cuts at: 2181 6762 2181
Size: 4581 2518
BspHI T'CATG_A
Cuts at: 4988 6718 4988
Size: 1730 5369
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2281 2990 3546 4454 844
Size: 1437 709 556 908 3489
Fragments arranged by size:
3489 1437 908 709 556
BsrBI CCG'CTC
Cuts at: 1394 1823 3268 4986 1394
Size: 429 1445 1718 3507
Fragments arranged by size:
3507 1718 1445 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5594 5768 65
Size: 1183 4346 174 1396
Fragments arranged by size:
4346 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3250 4140 5743 5930 1700
Size: 110 1440 890 1603 187 2869
Fragments arranged by size:
2869 1603 1440 890 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7099
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7099
BssSI C'ACGA_G
Cuts at: 5159 6171 5159
Size: 1012 6087
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2886 2961 2970 3211 794
Size: 2092 75 9 241 4682
Fragments arranged by size:
4682 2092 241 75 9
BstBI TT'CG_AA
Cuts at: 2007 5903 2007
Size: 3896 3203
BstEII G'GTnAC_C
Cuts at: 1184 3190 3785 4168 1184
Size: 2006 595 383 4115
Fragments arranged by size:
4115 2006 595 383
BstXI CCAn_nnnn'nTGG
Cuts at: 4109 4109
Size: 7099
BstYI r'GATC_y
Cuts at: 1972 2208 2334 2718 2916 3562 4429 4796 5181
Size: 236 126 384 198 646 867 367 385
Cuts at: 5181 5198 6650 6736 6748 7094 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 867 646 385 384 367 346
236 198 126 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2646 2871 2646
Size: 225 6874
BtgI C'CryG_G
Cuts at: 513 1065 2022 2261 2325 2733 3043 3153 513
Size: 552 957 239 64 408 310 110 4459
Fragments arranged by size:
4459 957 552 408 310 239 110 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6360
Fragments arranged by size:
6360 683 56
BtsI GCAGTG_nn'
Cuts at: 2062 3746 3931 4615 5409 5429 2062
Size: 1684 185 684 794 20 3732
Fragments arranged by size:
3732 1684 794 684 185 20
ClaI AT'CG_AT
Cuts at: 1554 3597 1554
Size: 2043 5056
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7064
DraI TTT'AAA
Cuts at: 4403 4403
Size: 7099
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4097 6106 809
Size: 507 2781 2009 1802
Fragments arranged by size:
2781 2009 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2414 2568 3657
Size: 54 1425 50 275 18 584 154 1089
Cuts at: 3657 3671 4148 4546 5435 7064 8
Size: 14 477 398 889 1629 43
Fragments arranged by size:
1629 1425 1089 889 584 477 398 275
154 54 50 43 18 14
EagI C'GGCC_G
Cuts at: 1537 1812 4546 1537
Size: 275 2734 4090
Fragments arranged by size:
4090 2734 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2022 2027 3317 3431 5029 1353
Size: 669 5 1290 114 1598 3423
Fragments arranged by size:
3423 1598 1290 669 114 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5686 6060 6206 387
Size: 1064 138 4097 374 146 1280
Fragments arranged by size:
4097 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2467 2467
Size: 7099
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2483 2555 2564 3341 3440 3503 3690
Size: 393 851 72 9 777 99 63 187
Cuts at: 3690 3867 4203 5162 5759 6546 6814 1239
Size: 177 336 959 597 787 268 1524
Fragments arranged by size:
1524 959 851 787 777 597 393 336
268 187 177 99 72 63 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2963 3185 3281 3705 2963
Size: 222 96 424 6357
Fragments arranged by size:
6357 424 222 96
EcoO109I rG'GnC_Cy
Cuts at: 2354 3124 3178 3445 3697 3766 4340 2354
Size: 770 54 267 252 69 574 5113
Fragments arranged by size:
5113 770 574 267 252 69 54
EcoRI G'AATT_C
Cuts at: 4408 4786 4408
Size: 378 6721
FspI TGC'GCA
Cuts at: 3200 5605 3200
Size: 2405 4694
HaeII r_GCGC'y
Cuts at: 1678 6246 1678
Size: 4568 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3128 3160 3964 3996 5740
Size: 32 135 32 1556 32 804 32 1744
Cuts at: 5740 5772 5814 5846 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1744 1556 804 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3295 4443 4689 669
Size: 988 281 1357 1148 246 3079
Fragments arranged by size:
3079 1357 1148 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7099
HpaI GTT'AAC
Cuts at: 4689 4689
Size: 7099
KpnI G_GTAC'C
Cuts at: 4424 4424
Size: 7099
MfeI C'AATT_G
Cuts at: 4698 4698
Size: 7099
MluI A'CGCG_T
Cuts at: 6975 6975
Size: 7099
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3950 6213 6397 1305
Size: 2645 2263 184 2007
Fragments arranged by size:
2645 2263 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2416 2570 3659 3673 10
Size: 54 1425 343 584 154 1089 14 3436
Fragments arranged by size:
3436 1425 1089 584 343 154 54 14
MslI CAynn'nnrTG
Cuts at: 518 1166 2107 2201 3659 5057 5416 5575 518
Size: 648 941 94 1458 1398 359 159 2042
Fragments arranged by size:
2042 1458 1398 941 648 359 159 94
MspA1I CmG'CkG
Cuts at: 1606 2742 2853 2940 3208 3349 3618 4223 4336
Size: 1136 111 87 268 141 269 605 113
Cuts at: 4336 4515 5192 6340 6585 1606
Size: 179 677 1148 245 2120
Fragments arranged by size:
2120 1148 1136 677 605 269 268 245
179 141 113 111 87
NaeI GCC'GGC
Cuts at: 3252 4142 3252
Size: 890 6209
NcoI C'CATG_G
Cuts at: 513 1065 2022 2261 513
Size: 552 957 239 5351
Fragments arranged by size:
5351 957 552 239
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7099
NgoMIV G'CCGG_C
Cuts at: 3250 4140 3250
Size: 890 6209
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7099
NotI GC'GGCC_GC
Cuts at: 4546 4546
Size: 7099
NspI r_CATG'y
Cuts at: 1132 1229 2712 2849 3033 4457 6002 1132
Size: 97 1483 137 184 1424 1545 2229
Fragments arranged by size:
2229 1545 1483 1424 184 137 97
PasI CC'CwG_GG
Cuts at: 1348 2764 1348
Size: 1416 5683
PciI A'CATG_T
Cuts at: 5998 5998
Size: 7099
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2566 2940 3049 1108
Size: 1458 374 109 5158
Fragments arranged by size:
5158 1458 374 109
PfoI T'CCnGG_A
Cuts at: 2211 3519 2211
Size: 1308 5791
PmeI GTTT'AAAC
Cuts at: 4403 4403
Size: 7099
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3074 3106 5168 5200 6681 6713 3074
Size: 32 2062 32 1481 32 3460
Fragments arranged by size:
3460 2062 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3178 3445 3697 3766 4340 3178
Size: 267 252 69 574 5937
Fragments arranged by size:
5937 574 267 252 69
PshAI GACnn'nnGTC
Cuts at: 1613 2634 1613
Size: 1021 6078
PsiI TTA'TAA
Cuts at: 4669 4669
Size: 7099
PspOMI G'GGCC_C
Cuts at: 3124 3124
Size: 7099
PstI C_TGCA'G
Cuts at: 830 2957 3216 3543 3735 4003 4120 4451 830
Size: 2127 259 327 192 268 117 331 3478
Fragments arranged by size:
3478 2127 331 327 268 259 192 117
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7099
PvuII CAG'CTG
Cuts at: 2742 2853 3618 4223 2742
Size: 111 765 605 5618
Fragments arranged by size:
5618 765 605 111
SacI G_AGCT'C
Cuts at: 2469 2469
Size: 7099
SalI G'TCGA_C
Cuts at: 1936 3293 4441 1936
Size: 1357 1148 4594
Fragments arranged by size:
4594 1357 1148
SanDI GG'GwC_CC
Cuts at: 3766 4340 3766
Size: 574 6525
SapI GCTCTTCn'nnn_
Cuts at: 3431 3431
Size: 7099
SbfI CC_TGCA'GG
Cuts at: 4451 4451
Size: 7099
ScaI AGT'ACT
Cuts at: 3311 5347 7059 3311
Size: 2036 1712 3351
Fragments arranged by size:
3351 2036 1712
SexAI A'CCwGG_T
Cuts at: 3781 3781
Size: 7099
SfcI C'TryA_G
Cuts at: 826 1045 2953 3212 3539 3731 3999 4116 4447
Size: 219 1908 259 327 192 268 117 331
Cuts at: 4447 5582 6263 6454 826
Size: 1135 681 191 1471
Fragments arranged by size:
1908 1471 1135 681 331 327 268 259
219 192 191 117
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7099
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7099
SmaI CCC'GGG
Cuts at: 1872 4346 4426 1872
Size: 2474 80 4545
Fragments arranged by size:
4545 2474 80
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2483 2723 5203 6104 6366 820
Size: 197 926 540 240 2480 901 262 1553
Fragments arranged by size:
2480 1553 926 901 540 262 240 197
SnaBI TAC'GTA
Cuts at: 493 4396 4890 493
Size: 3903 494 2702
Fragments arranged by size:
3903 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7099
SphI G_CATG'C
Cuts at: 1132 2712 4457 1132
Size: 1580 1745 3774
Fragments arranged by size:
3774 1745 1580
SspI AAT'ATT
Cuts at: 5 52 5023 5
Size: 47 4971 2081
Fragments arranged by size:
4971 2081 47
StuI AGG'CCT
Cuts at: 1517 3576 4195 1517
Size: 2059 619 4421
Fragments arranged by size:
4421 2059 619
StyI C'CwwG_G
Cuts at: 513 1065 2022 2261 4239 4538 513
Size: 552 957 239 1978 299 3074
Fragments arranged by size:
3074 1978 957 552 299 239
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2756 2830 3380 3446 5126 5143 5296
Size: 1255 1176 74 550 66 1680 17 153
Cuts at: 5296 5481 5926 325
Size: 185 445 1498
Fragments arranged by size:
1680 1498 1255 1176 550 445 185 153
74 66 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3309 5345 7057
Size: 274 80 33 51 1443 1332 2036 1712
Cuts at: 7057 96
Size: 138
Fragments arranged by size:
2036 1712 1443 1332 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2537 2569 3974 4006 7059 7091 2537
Size: 32 1405 32 3053 32 2545
Fragments arranged by size:
3053 2545 1405 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7099
XbaI T'CTAG_A
Cuts at: 4435 4435
Size: 7099
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2937 2937
Size: 7099
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7099
XmaI C'CCGG_G
Cuts at: 1870 4344 4424 1870
Size: 2474 80 4545
Fragments arranged by size:
4545 2474 80
XmnI GAAnn'nnTTC
Cuts at: 3425 5228 3425
Size: 1803 5296
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3403 4907 276
Size: 53 83 186 936 1869 1504 2468
Fragments arranged by size:
2468 1869 1504 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspXI PsrI RsrII SacII
SfiI SfoI SrfI SwaI