Restriction map for
pFN21ASDA0235
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AflIII AgeI AhdI Alw44I AocI
ApaLI AscI AspEI BbeI BbrPI
BbvCI BfrI BsaHI BsaWI BseAI
BsgI BsiWI BsmBI BspDI BspEI
BspLU11I BsrBI BsrFI BssHII BssSI
BstBI BstEII BstPI Bsu36I Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV FauI FseI
Hin1I HindIII KasI KspI MluI
MroI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI SwaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 729 GGTACC G^GTACC
Aor51HI 563 AGCGCT AGC^GCT
ApaI 570 GGGCCC GGGCC^C
AseI 2307 ATTAAT AT^TAAT
AsnI 2307 ATTAAT AT^TAAT
Asp718I 729 GGTACC G^GTACC
AspI 1625 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 35 CTCGGG C^[CT]CG[GA]G
AviII 3086 TGCGCA TGC^GCA
AvrII 3156 CCTAGG C^CTAGG
BalI 3116 TGGCCA TGG^CCA
BamHI 1933 GGATCC G^GATCC
BbsI 2800 GAAGAC GAAGAC(2/6)
BcgI 3057 GCACAAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1056 GTATCC GTATCC(6/5)
BclI 1323 TGATCA T^GATCA
BglI 1469 GCCTTACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3156 CCTAGG C^CTAGG
BpuAI 2800 GAAGAC GAAGAC(2/6)
BsaAI 3001 TACGTG [CT]AC^GT[GA]
BsaI 431 GAGACC GGTCTC(1/5)
BsiEI 2790 CGGTCG CG[GA][CT]^CG
BsmI 3185 GAATGC GAATGC(1/-1)
BsoBI 35 CTCGGG C^[CT]CG[GA]G
BspHI 6 TCATGA T^CATGA
Bst1107I 969 GTATAC GTA^TAC
BstZ17I 969 GTATAC GTA^TAC
Eco47III 563 AGCGCT AGC^GCT
EcoT22I 851 ATGCAT ATGCA^T
FbaI 1323 TGATCA T^GATCA
FspI 3086 TGCGCA TGC^GCA
HaeII 563 AGCGCT [GA]GCGC^[CT]
HgaI 1176 GCGTC GACGC(5/10)
HpaI 825 GTTAAC GTT^AAC
HphI 159 TCACC GGTGA(8/7)
KpnI 729 GGTACC GGTAC^C
MaeII 3002 ACGT A^CGT
MfeI 1502 CAATTG C^AATTG
MluNI 3116 TGGCCA TGG^CCA
MscI 3116 TGGCCA TGG^CCA
MunI 1502 CAATTG C^AATTG
NsiI 851 ATGCAT ATGCA^T
PflFI 1625 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3175 CCACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 3159 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 2307 ATTAAT AT^TAAT
RcaI 6 TCATGA T^CATGA
SacI 1373 GAGCTC GAGCT^C
SalI 2792 GTCGAC G^TCGAC
ScaI 1299 AGTACT AGT^ACT
SexAI 1626 ACCTGGT A^CC[AT]GGT
SmlI 2204 CTCAAG C^T[CT][GA]AG
Tsp45I 2586 GTCAC ^GT[GC]AC
Tth111I 1625 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3175 CCACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2221 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 969 GTATAC GT^[AC][GT]AC
2792 GTCGAC
AccII 248 CGCG CG^CG
2440 CGCG
AspHI 1373 GAGCTC G[AT]GC[AT]^C
2515 GTGCTC
BanI 729 GGTACC G^G[CT][GA]CC
1333 GGTGCC
BanII 570 GGGCCC G[GA]GC[CT]^C
1373 GAGCTC
BcnI 2078 CCCGG CC^[GC]GG
3150 CCCGG
BglII 196 AGATCT A^GATCT
1789 AGATCT
BsaBI 511 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1934 GATCCTCATC
BsiHKAI 1373 GAGCTC G[AT]GC[AT]^C
2515 GTGCTC
Bsp1407I 1781 TGTACA T^GTACA
2553 TGTACA
BsrDI 2680 CATTGC GCAATG(2/0)
2870 GCAATG
BsrGI 1781 TGTACA T^GTACA
2553 TGTACA
BstAPI 898 GCAGGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1426 GCACAAGCTGC
BstUI 248 CGCG CG^CG
2440 CGCG
BstXI 2943 CCAGAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2996 CCAATTACGTGG
DsaI 677 CCATGG C^C[GA][CT]GG
1045 CCATGG
EarI 605 CTCTTC CTCTTC(1/4)
741 CTCTTC
EcoNI 376 CCTGAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3163 CCTATTGGAGG
HapII 2079 CCGG C^CGG
3151 CCGG
HpaII 2079 CCGG C^CGG
3151 CCGG
Ksp632I 605 CTCTTC CTCTTC(1/4)
741 CTCTTC
MamI 511 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1934 GATCCTCATC
MslI 2641 CATCCTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2973 CACCATGATG
MspI 2079 CCGG C^CGG
3151 CCGG
MvnI 248 CGCG CG^CG
2440 CGCG
NciI 2078 CCCGG CC^[GC]GG
3150 CCCGG
NcoI 677 CCATGG C^CATGG
1045 CCATGG
SphI 1283 GCATGC GCATG^C
1868 GCATGC
SspBI 1781 TGTACA T^GTACA
2553 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AvaII BbvI
BfaI BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HhaI HinP1I HincII
HindII HinfI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII XmnI