(Circular) (Six-base) MAPSORT of: pFN21ASDA0235.seq Check: 2408 from: 1 to: 7921 pFN21ASDA0235 7921 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5731 278 Size: 53 83 186 936 4195 2468 Fragments arranged by size: 4195 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2993 4816 5264 1937 Size: 1056 1823 448 4594 Fragments arranged by size: 4594 1823 1056 448 Acc65I G'GTAC_C Cuts at: 2752 5242 2752 Size: 2490 5431 AclI AA'CG_TT Cuts at: 1563 6048 6421 1563 Size: 4485 373 3063 Fragments arranged by size: 4485 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2595 2751 4172 4842 5984 7368 7636 1632 Size: 963 156 1421 670 1142 1384 268 1917 Fragments arranged by size: 1917 1421 1384 1142 963 670 268 156 AfeI AGC'GCT Cuts at: 2588 2588 Size: 7921 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7724 AflIII A'CryG_T Cuts at: 1203 6820 7797 1203 Size: 5617 977 1327 Fragments arranged by size: 5617 1327 977 AgeI A'CCGG_T Cuts at: 6752 6752 Size: 7921 AhdI GACnn_n'nnGTC Cuts at: 6650 6650 Size: 7921 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7921 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2519 2889 3123 3590 3914 4725 1184 Size: 1335 370 234 467 324 811 4380 Fragments arranged by size: 4380 1335 811 467 370 324 234 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3387 3419 3387 Size: 32 7889 AlwNI CAG_nnn'CTG Cuts at: 2692 3473 4022 5664 7236 2692 Size: 781 549 1642 1572 3377 Fragments arranged by size: 3377 1642 1572 781 549 ApaI G_GGCC'C Cuts at: 2597 2597 Size: 7921 ApaLI G'TGCA_C Cuts at: 5978 7134 5978 Size: 1156 6765 ApoI r'AATT_y Cuts at: 1493 2365 2666 4308 4883 5230 5456 5608 7717 Size: 872 301 1642 575 347 226 152 2109 Cuts at: 7717 1493 Size: 1697 Fragments arranged by size: 2109 1697 1642 872 575 347 301 226 152 AseI AT'TA_AT Cuts at: 160 1032 4331 6475 160 Size: 872 3299 2144 1606 Fragments arranged by size: 3299 2144 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7921 AvaI C'yCGr_G Cuts at: 1870 1943 2058 5246 1870 Size: 73 115 3188 4545 Fragments arranged by size: 4545 3188 115 73 AvrII C'CTAG_G Cuts at: 5179 5179 Size: 7921 BamHI G'GATC_C Cuts at: 3956 5251 5618 3956 Size: 1295 367 6259 Fragments arranged by size: 6259 1295 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2752 3356 5242 6697 618 Size: 325 217 627 965 604 1886 1455 1842 Fragments arranged by size: 1886 1842 1455 965 627 604 325 217 BanII G_rGCy'C Cuts at: 1391 2597 3400 5240 1391 Size: 1206 803 1840 4072 Fragments arranged by size: 4072 1840 1206 803 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4830 928 Size: 971 2931 4019 Fragments arranged by size: 4019 2931 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5069 5103 6112 6146 1903 Size: 34 3132 34 1009 34 3678 Fragments arranged by size: 3678 3132 1009 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3090 5813 7023 3090 Size: 2723 1210 3988 Fragments arranged by size: 3988 2723 1210 BclI T'GATC_A Cuts at: 1548 3346 1548 Size: 1798 6123 BfrBI ATG'CAT Cuts at: 2876 2876 Size: 7921 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3498 6532 136 Size: 107 122 71 631 605 1826 3034 1525 Fragments arranged by size: 3034 1826 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2219 3812 7916 2219 Size: 1593 4104 2224 Fragments arranged by size: 4104 2224 1593 BlpI GC'TnA_GC Cuts at: 2889 3156 3715 4284 5338 2889 Size: 267 559 569 1054 5472 Fragments arranged by size: 5472 1054 569 559 267 Bme1580I G_kGCm'C Cuts at: 1790 2597 5982 7138 1790 Size: 807 3385 1156 2573 Fragments arranged by size: 3385 2573 1156 807 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7921 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2630 5652 6610 Size: 416 53 83 399 24 1213 3022 958 Cuts at: 6610 442 Size: 1753 Fragments arranged by size: 3022 1753 1213 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7921 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3798 4293 3798 Size: 495 7426 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2916 2961 3207 3384 3396 3495 3897 4044 Size: 1677 45 246 177 12 99 402 147 Cuts at: 4044 4060 4263 6581 1239 Size: 16 203 2318 2579 Fragments arranged by size: 2579 2318 1677 402 246 203 177 147 99 45 16 12 Bpu10I CC'TnA_GC Cuts at: 4438 4438 Size: 7921 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4212 6046 6911 7209 4212 Size: 1834 865 298 4924 Fragments arranged by size: 4924 1834 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2448 6584 882 Size: 617 949 4136 2219 Fragments arranged by size: 4136 2219 949 617 BsaAI yAC'GTr Cuts at: 493 1204 5026 5218 5712 493 Size: 711 3822 192 494 2702 Fragments arranged by size: 3822 2702 711 494 192 BsaBI GATnn'nnATC Cuts at: 2538 3961 7915 2538 Size: 1423 3954 2544 Fragments arranged by size: 3954 2544 1423 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5728 6110 6745 275 Size: 53 83 186 936 4195 382 635 1451 Fragments arranged by size: 4195 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6354 6752 7026 7173 7584 6354 Size: 398 274 147 411 6691 Fragments arranged by size: 6691 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2763 2793 3162 3192 3387 Size: 30 165 30 1360 30 369 30 195 Cuts at: 3387 3417 1178 Size: 30 5682 Fragments arranged by size: 5682 1360 369 195 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2453 2964 3862 1186 Size: 1267 511 898 5245 Fragments arranged by size: 5245 1267 898 511 BseYI C'CCAG_C Cuts at: 2196 2802 7124 7620 2196 Size: 606 4322 496 2497 Fragments arranged by size: 4322 2497 606 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7348 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4816 5371 6132 7160 1540 Size: 275 186 2815 555 761 1028 2301 Fragments arranged by size: 2815 2301 1028 761 555 275 186 BsiHKAI G_wGCw'C Cuts at: 3400 4542 5982 6067 7138 3400 Size: 1142 1440 85 1071 4183 Fragments arranged by size: 4183 1440 1142 1071 85 BsmI GAATG_Cn' Cuts at: 5214 5432 5525 5214 Size: 218 93 7610 Fragments arranged by size: 7610 218 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2597 3400 4542 5240 5982 6067 7138 Size: 399 807 803 1142 698 742 85 1071 Cuts at: 7138 1391 Size: 2174 Fragments arranged by size: 2174 1142 1071 807 803 742 698 399 85 BspEI T'CCGG_A Cuts at: 7584 7584 Size: 7921 BspHI T'CATG_A Cuts at: 2029 5810 7540 2029 Size: 3781 1730 2410 Fragments arranged by size: 3781 2410 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3020 3182 5065 5276 844 Size: 2176 162 1883 211 3489 Fragments arranged by size: 3489 2176 1883 211 162 BsrBI CCG'CTC Cuts at: 1394 1823 5808 1394 Size: 429 3985 3507 Fragments arranged by size: 3985 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4702 4900 6416 6590 65 Size: 1183 3454 198 1516 174 1396 Fragments arranged by size: 3454 1516 1396 1183 198 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6565 6752 1700 Size: 110 4755 187 2869 Fragments arranged by size: 4755 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3804 4576 96 Size: 3708 772 3441 Fragments arranged by size: 3708 3441 772 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7921 BssSI C'ACGA_G Cuts at: 5981 6993 5981 Size: 1012 6909 BstAPI GCAn_nnn'nTGC Cuts at: 794 2927 3455 794 Size: 2133 528 5260 Fragments arranged by size: 5260 2133 528 BstBI TT'CG_AA Cuts at: 2007 6725 2007 Size: 4718 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7921 BstXI CCAn_nnnn'nTGG Cuts at: 4973 5026 4973 Size: 53 7868 BstYI r'GATC_y Cuts at: 1972 2219 3812 3956 5251 5618 6003 6020 7472 Size: 247 1593 144 1295 367 385 17 1452 Cuts at: 7472 7558 7570 7916 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1593 1452 1295 385 367 346 247 144 86 17 12 BstZ17I GTA'TAC Cuts at: 2994 2994 Size: 7921 BtgI C'CryG_G Cuts at: 513 1065 2700 3068 513 Size: 552 1635 368 5366 Fragments arranged by size: 5366 1635 552 368 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7182 Fragments arranged by size: 7182 683 56 BtsI GCAGTG_nn' Cuts at: 3045 4707 5437 6231 6251 3045 Size: 1662 730 794 20 4715 Fragments arranged by size: 4715 1662 794 730 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7921 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7886 DraI TTT'AAA Cuts at: 5225 5225 Size: 7921 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6928 809 Size: 507 5612 1802 Fragments arranged by size: 5612 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3521 4138 5139 Size: 54 1425 50 275 18 1691 617 1001 Cuts at: 5139 5368 6257 7886 8 Size: 229 889 1629 43 Fragments arranged by size: 1691 1629 1425 1001 889 617 275 229 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5368 1537 Size: 275 3556 4090 Fragments arranged by size: 4090 3556 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2634 2770 5851 1353 Size: 1281 136 3081 3423 Fragments arranged by size: 3423 3081 1281 136 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6508 6882 7028 387 Size: 1064 138 4919 374 146 1280 Fragments arranged by size: 4919 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3398 3398 Size: 7921 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2595 2751 2916 2961 3207 3384 3396 Size: 393 963 156 165 45 246 177 12 Cuts at: 3396 3495 3897 4044 4060 4172 4263 4842 5984 Size: 99 402 147 16 112 91 579 1142 Cuts at: 5984 6581 7368 7636 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1142 963 787 597 579 402 393 268 246 177 165 156 147 112 99 91 45 16 12 EcoNI CCTnn'n_nnAGG Cuts at: 2403 5190 2403 Size: 2787 5134 EcoO109I rG'GnC_Cy Cuts at: 2594 2624 5183 2594 Size: 30 2559 5332 Fragments arranged by size: 5332 2559 30 EcoRI G'AATT_C Cuts at: 5230 5608 5230 Size: 378 7543 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3621 3621 Size: 7921 FspI TGC'GCA Cuts at: 5111 6427 5111 Size: 1316 6605 HaeII r_GCGC'y Cuts at: 1678 2590 7068 1678 Size: 912 4478 2531 Fragments arranged by size: 4478 2531 912 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3770 3802 4375 4407 5077 Size: 32 135 32 2198 32 573 32 670 Cuts at: 5077 5109 6562 6594 6636 6668 1373 Size: 32 1453 32 42 32 2626 Fragments arranged by size: 2626 2198 1453 670 573 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2850 3656 4817 4877 5265 5511 Size: 988 281 912 806 1161 60 388 246 Cuts at: 5511 669 Size: 3079 Fragments arranged by size: 3079 1161 988 912 806 388 281 246 60 HindIII A'AGCT_T Cuts at: 748 748 Size: 7921 HpaI GTT'AAC Cuts at: 2850 5511 2850 Size: 2661 5260 KpnI G_GTAC'C Cuts at: 2756 5246 2756 Size: 2490 5431 MfeI C'AATT_G Cuts at: 3525 5520 3525 Size: 1995 5926 MluI A'CGCG_T Cuts at: 7797 7797 Size: 7921 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2594 3296 3356 3443 5127 7035 7219 1305 Size: 1289 702 60 87 1684 1908 184 2007 Fragments arranged by size: 2007 1908 1684 1289 702 184 87 60 MscI TGG'CCA Cuts at: 10 64 1489 1832 5141 10 Size: 54 1425 343 3309 2790 Fragments arranged by size: 3309 2790 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4668 5000 5879 6238 6397 518 Size: 648 3502 332 879 359 159 2042 Fragments arranged by size: 3502 2042 879 648 359 332 159 MspA1I CmG'CkG Cuts at: 1606 2936 3113 3257 3473 4283 5337 6014 7162 Size: 1330 177 144 216 810 1054 677 1148 Cuts at: 7162 7407 1606 Size: 245 2120 Fragments arranged by size: 2120 1330 1148 1054 810 677 245 216 177 144 NcoI C'CATG_G Cuts at: 513 1065 2700 3068 513 Size: 552 1635 368 5366 Fragments arranged by size: 5366 1635 552 368 NdeI CA'TA_TG Cuts at: 387 387 Size: 7921 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7921 NotI GC'GGCC_GC Cuts at: 5368 5368 Size: 7921 NsiI A_TGCA'T Cuts at: 2878 2878 Size: 7921 NspI r_CATG'y Cuts at: 1132 1229 3310 3895 4802 5279 6824 1132 Size: 97 2081 585 907 477 1545 2229 Fragments arranged by size: 2229 2081 1545 907 585 477 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7921 PciI A'CATG_T Cuts at: 6820 6820 Size: 7921 PflMI CCAn_nnn'nTGG Cuts at: 1108 5204 1108 Size: 4096 3825 PfoI T'CCnGG_A Cuts at: 2548 3913 2548 Size: 1365 6556 PmeI GTTT'AAAC Cuts at: 5225 5225 Size: 7921 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3387 3419 5990 6022 7503 7535 3387 Size: 32 2571 32 1481 32 3773 Fragments arranged by size: 3773 2571 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 5183 5183 Size: 7921 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7921 PsiI TTA'TAA Cuts at: 5491 5491 Size: 7921 PspOMI G'GGCC_C Cuts at: 2593 2593 Size: 7921 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4707 4739 4707 Size: 32 7889 PstI C_TGCA'G Cuts at: 830 2857 3001 3016 3112 3262 3460 4018 4679 Size: 2027 144 15 96 150 198 558 661 Cuts at: 4679 4714 5273 830 Size: 35 559 3478 Fragments arranged by size: 3478 2027 661 559 558 198 150 144 96 35 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7921 PvuII CAG'CTG Cuts at: 2936 3113 3257 3473 4283 2936 Size: 177 144 216 810 6574 Fragments arranged by size: 6574 810 216 177 144 SacI G_AGCT'C Cuts at: 3400 3400 Size: 7921 SalI G'TCGA_C Cuts at: 1936 4815 5263 1936 Size: 2879 448 4594 Fragments arranged by size: 4594 2879 448 SbfI CC_TGCA'GG Cuts at: 5273 5273 Size: 7921 ScaI AGT'ACT Cuts at: 3324 6169 7881 3324 Size: 2845 1712 3364 Fragments arranged by size: 3364 2845 1712 SexAI A'CCwGG_T Cuts at: 3649 3649 Size: 7921 SfcI C'TryA_G Cuts at: 826 1045 2640 2778 2853 2997 3012 3108 3258 Size: 219 1595 138 75 144 15 96 150 Cuts at: 3258 3456 3708 4014 4613 4675 4710 5269 6404 Size: 198 252 306 599 62 35 559 1135 Cuts at: 6404 7085 7276 826 Size: 681 191 1471 Fragments arranged by size: 1595 1471 1135 681 599 559 306 252 219 198 191 150 144 138 96 75 62 35 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7921 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7921 SmaI CCC'GGG Cuts at: 1872 5248 1872 Size: 3376 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4227 6025 6926 7188 820 Size: 197 926 2284 1798 901 262 1553 Fragments arranged by size: 2284 1798 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5218 5712 493 Size: 4725 494 2702 Fragments arranged by size: 4725 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7921 SphI G_CATG'C Cuts at: 1132 3310 3895 5279 1132 Size: 2178 585 1384 3774 Fragments arranged by size: 3774 2178 1384 585 SspI AAT'ATT Cuts at: 5 52 5845 5 Size: 47 5793 2081 Fragments arranged by size: 5793 2081 47 StuI AGG'CCT Cuts at: 1517 2289 2784 2925 1517 Size: 772 495 141 6513 Fragments arranged by size: 6513 772 495 141 StyI C'CwwG_G Cuts at: 513 1065 2290 2700 3068 3391 5179 5360 513 Size: 552 1225 410 368 323 1788 181 3074 Fragments arranged by size: 3074 1788 1225 552 410 368 323 181 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2999 3689 3847 5948 5965 6118 6303 Size: 1255 1419 690 158 2101 17 153 185 Cuts at: 6303 6748 325 Size: 445 1498 Fragments arranged by size: 2101 1498 1419 1255 690 445 185 158 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3322 3665 3804 Size: 274 80 33 51 1443 1345 343 139 Cuts at: 3804 4576 4801 6167 7879 96 Size: 772 225 1366 1712 138 Fragments arranged by size: 1712 1443 1366 1345 772 343 274 225 139 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7881 7913 7881 Size: 32 7889 Tth111I GACn'n_nGTC Cuts at: 1316 3651 1316 Size: 2335 5586 XbaI T'CTAG_A Cuts at: 4244 5257 4244 Size: 1013 6908 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7921 XmaI C'CCGG_G Cuts at: 1870 5246 1870 Size: 3376 4545 XmnI GAAnn'nnTTC Cuts at: 3601 4048 4596 6050 3601 Size: 447 548 1454 5472 Fragments arranged by size: 5472 1454 548 447 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5729 276 Size: 53 83 186 936 4195 2468 Fragments arranged by size: 4195 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI BaeI BbeI BbvCI BsiWI BsmBI Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI