Restriction map for
pFN21ASDA0235
(Linear) (Six-base) MAPSORT of: KIAA0235.seq Check: 2475 from: 1 to: 3192
KIAA0235.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AccI GT'mk_AC
Cuts at: 0 970 2793 3192
Size: 970 1823 399
Fragments arranged by size:
1823 970 399
Acc65I G'GTAC_C
Cuts at: 0 729 3192
Size: 729 2463
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 728 2149 2819 3192
Size: 572 156 1421 670 373
Fragments arranged by size:
1421 670 572 373 156
AfeI AGC'GCT
Cuts at: 0 565 3192
Size: 565 2627
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 496 866 1100 1567 1891 2702 3192
Size: 496 370 234 467 324 811 490
Fragments arranged by size:
811 496 490 467 370 324 234
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1364 1396 3192
Size: 1364 32 1796
Fragments arranged by size:
1796 1364 32
AlwNI CAG_nnn'CTG
Cuts at: 0 669 1450 1999 3192
Size: 669 781 549 1193
Fragments arranged by size:
1193 781 669 549
ApaI G_GGCC'C
Cuts at: 0 574 3192
Size: 574 2618
ApoI r'AATT_y
Cuts at: 0 342 643 2285 2860 3192
Size: 342 301 1642 575 332
Fragments arranged by size:
1642 575 342 332 301
AseI AT'TA_AT
Cuts at: 0 2308 3192
Size: 2308 884
AvaI C'yCGr_G
Cuts at: 0 35 3192
Size: 35 3157
AvrII C'CTAG_G
Cuts at: 0 3156 3192
Size: 3156 36
BamHI G'GATC_C
Cuts at: 0 1933 3192
Size: 1933 1259
BanI G'GyrC_C
Cuts at: 0 729 1333 3192
Size: 729 604 1859
Fragments arranged by size:
1859 729 604
BanII G_rGCy'C
Cuts at: 0 574 1377 3192
Size: 574 803 1815
Fragments arranged by size:
1815 803 574
BbsI GAAGACnn'nnnn_
Cuts at: 0 2807 3192
Size: 2807 385
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3046 3080 3192
Size: 3046 34 112
Fragments arranged by size:
3046 112 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1067 3192
Size: 1067 2125
BclI T'GATC_A
Cuts at: 0 1323 3192
Size: 1323 1869
BfrBI ATG'CAT
Cuts at: 0 853 3192
Size: 853 2339
BglI GCCn_nnn'nGGC
Cuts at: 0 1475 3192
Size: 1475 1717
BglII A'GATC_T
Cuts at: 0 196 1789 3192
Size: 196 1593 1403
Fragments arranged by size:
1593 1403 196
BlpI GC'TnA_GC
Cuts at: 0 866 1133 1692 2261 3192
Size: 866 267 559 569 931
Fragments arranged by size:
931 866 569 559 267
Bme1580I G_kGCm'C
Cuts at: 0 574 3192
Size: 574 2618
BmrI ACTGGGnnnn_n'
Cuts at: 0 607 3192
Size: 607 2585
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1775 2270 3192
Size: 1775 495 922
Fragments arranged by size:
1775 922 495
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 893 938 1184 1361 1373 1472 1874 2021
Size: 893 45 246 177 12 99 402 147
Cuts at: 2021 2037 2240 3192
Size: 16 203 952
Fragments arranged by size:
952 893 402 246 203 177 147 99
45 16 12
Bpu10I CC'TnA_GC
Cuts at: 0 2415 3192
Size: 2415 777
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2189 3192
Size: 2189 1003
BsaI GGTCTCn'nnnn_
Cuts at: 0 425 3192
Size: 425 2767
BsaAI yAC'GTr
Cuts at: 0 3003 3192
Size: 3003 189
BsaBI GATnn'nnATC
Cuts at: 0 515 1938 3192
Size: 515 1423 1254
Fragments arranged by size:
1423 1254 515
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 740 770 1139 1169 1364 1394 3192
Size: 740 30 369 30 195 30 1798
Fragments arranged by size:
1798 740 369 195 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 430 941 1839 3192
Size: 430 511 898 1353
Fragments arranged by size:
1353 898 511 430
BseYI C'CCAG_C
Cuts at: 0 173 779 3192
Size: 173 606 2413
Fragments arranged by size:
2413 606 173
BsiEI CG_ry'CG
Cuts at: 0 2793 3192
Size: 2793 399
BsiHKAI G_wGCw'C
Cuts at: 0 1377 2519 3192
Size: 1377 1142 673
Fragments arranged by size:
1377 1142 673
BsmI GAATG_Cn'
Cuts at: 0 3191 3192
Size: 3191 1
Bsp1286I G_dGCh'C
Cuts at: 0 574 1377 2519 3192
Size: 574 803 1142 673
Fragments arranged by size:
1142 803 673 574
BspHI T'CATG_A
Cuts at: 0 6 3192
Size: 6 3186
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 997 1159 3042 3192
Size: 997 162 1883 150
Fragments arranged by size:
1883 997 162 150
BsrDI GCAATG_nn'
Cuts at: 0 2679 2877 3192
Size: 2679 198 315
Fragments arranged by size:
2679 315 198
BsrGI T'GTAC_A
Cuts at: 0 1781 2553 3192
Size: 1781 772 639
Fragments arranged by size:
1781 772 639
BstAPI GCAn_nnn'nTGC
Cuts at: 0 904 1432 3192
Size: 904 528 1760
Fragments arranged by size:
1760 904 528
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2950 3003 3192
Size: 2950 53 189
Fragments arranged by size:
2950 189 53
BstYI r'GATC_y
Cuts at: 0 196 1789 1933 3192
Size: 196 1593 144 1259
Fragments arranged by size:
1593 1259 196 144
BstZ17I GTA'TAC
Cuts at: 0 971 3192
Size: 971 2221
BtgI C'CryG_G
Cuts at: 0 677 1045 3192
Size: 677 368 2147
Fragments arranged by size:
2147 677 368
BtsI GCAGTG_nn'
Cuts at: 0 1022 2684 3192
Size: 1022 1662 508
Fragments arranged by size:
1662 1022 508
EaeI y'GGCC_r
Cuts at: 0 1498 2115 3116 3192
Size: 1498 617 1001 76
Fragments arranged by size:
1498 1001 617 76
EarI CTCTTCn'nnn_
Cuts at: 0 611 747 3192
Size: 611 136 2445
Fragments arranged by size:
2445 611 136
EcoICRI GAG'CTC
Cuts at: 0 1375 3192
Size: 1375 1817
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 728 893 938 1184 1361 1373 1472
Size: 572 156 165 45 246 177 12 99
Cuts at: 1472 1874 2021 2037 2149 2240 2819 3192
Size: 402 147 16 112 91 579 373
Fragments arranged by size:
579 572 402 373 246 177 165 156
147 112 99 91 45 16 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 380 3167 3192
Size: 380 2787 25
Fragments arranged by size:
2787 380 25
EcoO109I rG'GnC_Cy
Cuts at: 0 571 601 3160 3192
Size: 571 30 2559 32
Fragments arranged by size:
2559 571 32 30
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1598 3192
Size: 1598 1594
FspI TGC'GCA
Cuts at: 0 3088 3192
Size: 3088 104
HaeII r_GCGC'y
Cuts at: 0 567 3192
Size: 567 2625
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1747 1779 2352 2384 3054 3086 3192
Size: 1747 32 573 32 670 32 106
Fragments arranged by size:
1747 670 573 106 32 32 32
HincII GTy'rAC
Cuts at: 0 827 1633 2794 2854 3192
Size: 827 806 1161 60 338
Fragments arranged by size:
1161 827 806 338 60
HpaI GTT'AAC
Cuts at: 0 827 3192
Size: 827 2365
KpnI G_GTAC'C
Cuts at: 0 733 3192
Size: 733 2459
MfeI C'AATT_G
Cuts at: 0 1502 3192
Size: 1502 1690
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 1273 1333 1420 3104 3192
Size: 571 702 60 87 1684 88
Fragments arranged by size:
1684 702 571 88 87 60
MscI TGG'CCA
Cuts at: 0 3118 3192
Size: 3118 74
MslI CAynn'nnrTG
Cuts at: 0 2645 2977 3192
Size: 2645 332 215
Fragments arranged by size:
2645 332 215
MspA1I CmG'CkG
Cuts at: 0 913 1090 1234 1450 2260 3192
Size: 913 177 144 216 810 932
Fragments arranged by size:
932 913 810 216 177 144
NcoI C'CATG_G
Cuts at: 0 677 1045 3192
Size: 677 368 2147
Fragments arranged by size:
2147 677 368
NsiI A_TGCA'T
Cuts at: 0 855 3192
Size: 855 2337
NspI r_CATG'y
Cuts at: 0 1287 1872 2779 3192
Size: 1287 585 907 413
Fragments arranged by size:
1287 907 585 413
PflMI CCAn_nnn'nTGG
Cuts at: 0 3181 3192
Size: 3181 11
PfoI T'CCnGG_A
Cuts at: 0 525 1890 3192
Size: 525 1365 1302
Fragments arranged by size:
1365 1302 525
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1364 1396 3192
Size: 1364 32 1796
Fragments arranged by size:
1796 1364 32
PpuMI rG'GwC_Cy
Cuts at: 0 3160 3192
Size: 3160 32
PspOMI G'GGCC_C
Cuts at: 0 570 3192
Size: 570 2622
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2684 2716 3192
Size: 2684 32 476
Fragments arranged by size:
2684 476 32
PstI C_TGCA'G
Cuts at: 0 834 978 993 1089 1239 1437 1995 2656
Size: 834 144 15 96 150 198 558 661
Cuts at: 2656 2691 3192
Size: 35 501
Fragments arranged by size:
834 661 558 501 198 150 144 96
35 15
PvuII CAG'CTG
Cuts at: 0 913 1090 1234 1450 2260 3192
Size: 913 177 144 216 810 932
Fragments arranged by size:
932 913 810 216 177 144
SacI G_AGCT'C
Cuts at: 0 1377 3192
Size: 1377 1815
SalI G'TCGA_C
Cuts at: 0 2792 3192
Size: 2792 400
ScaI AGT'ACT
Cuts at: 0 1301 3192
Size: 1301 1891
SexAI A'CCwGG_T
Cuts at: 0 1626 3192
Size: 1626 1566
SfcI C'TryA_G
Cuts at: 0 617 755 830 974 989 1085 1235 1433
Size: 617 138 75 144 15 96 150 198
Cuts at: 1433 1685 1991 2590 2652 2687 3192
Size: 252 306 599 62 35 505
Fragments arranged by size:
617 599 505 306 252 198 150 144
138 96 75 62 35 15
SmlI C'TyrA_G
Cuts at: 0 2204 3192
Size: 2204 988
SphI G_CATG'C
Cuts at: 0 1287 1872 3192
Size: 1287 585 1320
Fragments arranged by size:
1320 1287 585
StuI AGG'CCT
Cuts at: 0 266 761 902 3192
Size: 266 495 141 2290
Fragments arranged by size:
2290 495 266 141
StyI C'CwwG_G
Cuts at: 0 267 677 1045 1368 3156 3192
Size: 267 410 368 323 1788 36
Fragments arranged by size:
1788 410 368 323 267 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 976 1666 1824 3192
Size: 976 690 158 1368
Fragments arranged by size:
1368 976 690 158
TatI w'GTAC_w
Cuts at: 0 1299 1642 1781 2553 2778 3192
Size: 1299 343 139 772 225 414
Fragments arranged by size:
1299 772 414 343 225 139
Tth111I GACn'n_nGTC
Cuts at: 0 1628 3192
Size: 1628 1564
XbaI T'CTAG_A
Cuts at: 0 2221 3192
Size: 2221 971
XmnI GAAnn'nnTTC
Cuts at: 0 1578 2025 2573 3192
Size: 1578 447 548 619
Fragments arranged by size:
1578 619 548 447
Enzymes that do cut:
AccI Acc65I AcuI AfeI AlfI AloI AlwNI ApaI
ApoI AseI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrDI BsrGI BstAPI BstXI BstYI BstZ17I BtgI
BtsI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I HincII HpaI KpnI MfeI MmeI
MscI MslI MspA1I NcoI NsiI NspI PflMI PfoI
PpiI PpuMI PspOMI PsrI PstI PvuII SacI SalI
ScaI SexAI SfcI SmlI SphI StuI StyI TaqII
TatI Tth111I XbaI XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AflIII AgeI AhdI AleI
ApaLI AscI AsiSI BaeI BbeI BbvCI BmgBI BmtI
BsaHI BsaWI BsgI BsiWI BsmBI BspEI BsrBI BsrFI
BssHII BssSI BstBI BstEII Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoRI EcoRV FseI
FspAI HindIII KasI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PmeI PmlI
PshAI PsiI PspXI PvuI RsrII SacII SanDI SapI
SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI TstI XcmI XhoI XmaI ZraI