(Circular) (Six-base) MAPSORT of: pFN21ASDA0218.seq Check: 4036 from: 1 to: 7012 pFN21ASDA0218 7012 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4822 278 Size: 53 83 186 936 3286 2468 Fragments arranged by size: 3286 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4355 1937 Size: 2418 4594 Acc65I G'GTAC_C Cuts at: 4333 4333 Size: 7012 AclI AA'CG_TT Cuts at: 1563 5139 5512 1563 Size: 3576 373 3063 Fragments arranged by size: 3576 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2340 2508 2606 3069 4046 5075 6459 6727 Size: 708 168 98 463 977 1029 1384 268 Cuts at: 6727 1632 Size: 1917 Fragments arranged by size: 1917 1384 1029 977 708 463 268 168 98 AflII C'TTAA_G Cuts at: 820 1017 2432 3769 820 Size: 197 1415 1337 4063 Fragments arranged by size: 4063 1415 1337 197 AflIII A'CryG_T Cuts at: 1203 4041 5911 6888 1203 Size: 2838 1870 977 1327 Fragments arranged by size: 2838 1870 1327 977 AgeI A'CCGG_T Cuts at: 5843 5843 Size: 7012 AhdI GACnn_n'nnGTC Cuts at: 5741 5741 Size: 7012 AleI CACnn'nnGTG Cuts at: 1166 4061 1166 Size: 2895 4117 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7012 AlwNI CAG_nnn'CTG Cuts at: 2753 4755 6327 2753 Size: 2002 1572 3438 Fragments arranged by size: 3438 2002 1572 ApaI G_GGCC'C Cuts at: 2169 2294 2169 Size: 125 6887 ApaLI G'TGCA_C Cuts at: 3826 5069 6225 3826 Size: 1243 1156 4613 Fragments arranged by size: 4613 1243 1156 ApoI r'AATT_y Cuts at: 1493 2477 3352 3582 3608 4321 4547 4699 6808 Size: 984 875 230 26 713 226 152 2109 Cuts at: 6808 1493 Size: 1697 Fragments arranged by size: 2109 1697 984 875 713 230 226 152 26 AseI AT'TA_AT Cuts at: 160 1032 5566 160 Size: 872 4534 1606 Fragments arranged by size: 4534 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7012 AvaI C'yCGr_G Cuts at: 1870 1943 2340 2646 4092 4198 4337 1870 Size: 73 397 306 1446 106 139 4545 Fragments arranged by size: 4545 1446 397 306 139 106 73 BamHI G'GATC_C Cuts at: 2363 4342 4709 2363 Size: 1979 367 4666 Fragments arranged by size: 4666 1979 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3774 4333 5788 618 Size: 325 217 627 1987 559 1455 1842 Fragments arranged by size: 1987 1842 1455 627 559 325 217 BanII G_rGCy'C Cuts at: 1391 2109 2169 2240 2294 2570 2883 4020 4331 Size: 718 60 71 54 276 313 1137 311 Cuts at: 4331 1391 Size: 4072 Fragments arranged by size: 4072 1137 718 313 311 276 71 60 54 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2346 928 Size: 971 447 5594 Fragments arranged by size: 5594 971 447 BbvCI CC'TCA_GC Cuts at: 2493 2493 Size: 7012 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5203 5237 1903 Size: 34 3266 34 3678 Fragments arranged by size: 3678 3266 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4904 6114 4904 Size: 1210 5802 BclI T'GATC_A Cuts at: 1548 1548 Size: 7012 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2648 4088 5623 Size: 107 122 71 631 605 976 1440 1535 Cuts at: 5623 136 Size: 1525 Fragments arranged by size: 1535 1525 1440 976 631 605 122 107 71 BglII A'GATC_T Cuts at: 3036 3931 7007 3036 Size: 895 3076 3041 Fragments arranged by size: 3076 3041 895 BlpI GC'TnA_GC Cuts at: 4429 4429 Size: 7012 Bme1580I G_kGCm'C Cuts at: 1790 2169 2294 3830 3963 5073 6229 1790 Size: 379 125 1536 133 1110 1156 2573 Fragments arranged by size: 2573 1536 1156 1110 379 133 125 BmgBI CAC'GTC Cuts at: 1318 2067 1318 Size: 749 6263 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2865 4743 5701 Size: 416 53 83 399 24 1448 1878 958 Cuts at: 5701 442 Size: 1753 Fragments arranged by size: 1878 1753 1448 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7012 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3019 3055 3019 Size: 36 6976 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2077 2110 2181 3116 3298 3358 3503 4139 Size: 838 33 71 935 182 60 145 636 Cuts at: 4139 5672 1239 Size: 1533 2579 Fragments arranged by size: 2579 1533 935 838 636 182 145 71 60 33 Bpu10I CC'TnA_GC Cuts at: 2493 2493 Size: 7012 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2844 4123 5137 6002 6300 2844 Size: 1279 1014 865 298 3556 Fragments arranged by size: 3556 1279 1014 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2553 5675 882 Size: 617 1054 3122 2219 Fragments arranged by size: 3122 2219 1054 617 BsaAI yAC'GTr Cuts at: 493 1204 4309 4803 493 Size: 711 3105 494 2702 Fragments arranged by size: 3105 2702 711 494 BsaBI GATnn'nnATC Cuts at: 3930 7006 3930 Size: 3076 3936 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2027 4819 5201 5836 Size: 53 83 186 936 494 2792 382 635 Cuts at: 5836 275 Size: 1451 Fragments arranged by size: 2792 1451 936 635 494 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5445 5843 6117 6264 6675 5445 Size: 398 274 147 411 5782 Fragments arranged by size: 5782 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2249 2279 2552 2582 3578 Size: 30 165 30 846 30 273 30 996 Cuts at: 3578 3608 3659 3689 4129 4159 1178 Size: 30 51 30 440 30 4031 Fragments arranged by size: 4031 996 846 440 273 165 51 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2251 2254 2257 2429 2813 2836 2891 3047 Size: 1065 3 3 172 384 23 55 156 Cuts at: 3047 3126 3437 3512 3683 4066 1186 Size: 79 311 75 171 383 4132 Fragments arranged by size: 4132 1065 384 383 311 172 171 156 79 75 55 23 3 3 BseYI C'CCAG_C Cuts at: 2168 2675 6215 6711 2168 Size: 507 3540 496 2469 Fragments arranged by size: 3540 2469 507 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6439 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4462 5223 6251 1540 Size: 275 186 2461 761 1028 2301 Fragments arranged by size: 2461 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3000 3830 5073 5158 6229 3000 Size: 830 1243 85 1071 3783 Fragments arranged by size: 3783 1243 1071 830 85 BsmI GAATG_Cn' Cuts at: 4523 4616 4523 Size: 93 6919 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2109 2169 2240 2294 2570 2883 3000 Size: 399 319 60 71 54 276 313 117 Cuts at: 3000 3830 3963 4020 4331 5073 5158 6229 1391 Size: 830 133 57 311 742 85 1071 2174 Fragments arranged by size: 2174 1071 830 742 399 319 313 311 276 133 117 85 71 60 57 54 BspEI T'CCGG_A Cuts at: 6675 6675 Size: 7012 BspHI T'CATG_A Cuts at: 3945 4901 6631 3945 Size: 956 1730 4326 Fragments arranged by size: 4326 1730 956 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4367 844 Size: 3523 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2035 4899 1394 Size: 429 212 2864 3507 Fragments arranged by size: 3507 2864 429 212 BsrDI GCAATG_nn' Cuts at: 65 1248 3985 5507 5681 65 Size: 1183 2737 1522 174 1396 Fragments arranged by size: 2737 1522 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2130 3995 5656 5843 1700 Size: 110 320 1865 1661 187 2869 Fragments arranged by size: 2869 1865 1661 320 187 110 BsrGI T'GTAC_A Cuts at: 96 3144 96 Size: 3048 3964 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7012 BssSI C'ACGA_G Cuts at: 5072 6084 5072 Size: 1012 6000 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7012 BstBI TT'CG_AA Cuts at: 2007 5816 2007 Size: 3809 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7012 BstXI CCAn_nnnn'nTGG Cuts at: 3322 3322 Size: 7012 BstYI r'GATC_y Cuts at: 1972 2363 3036 3685 3931 3975 4110 4342 4709 Size: 391 673 649 246 44 135 232 367 Cuts at: 4709 5094 5111 6563 6649 6661 7007 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 673 649 391 385 367 346 246 232 135 86 44 17 12 Bsu36I CC'TnA_GG Cuts at: 3603 3780 3603 Size: 177 6835 BtgI C'CryG_G Cuts at: 513 1065 2022 2680 513 Size: 552 957 658 4845 Fragments arranged by size: 4845 957 658 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6273 Fragments arranged by size: 6273 683 56 BtsI GCAGTG_nn' Cuts at: 2751 3910 4068 4528 5322 5342 2751 Size: 1159 158 460 794 20 4421 Fragments arranged by size: 4421 1159 794 460 158 20 ClaI AT'CG_AT Cuts at: 1554 2615 1554 Size: 1061 5951 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2268 2303 529 Size: 35 1704 35 5238 Fragments arranged by size: 5238 1704 35 35 DraI TTT'AAA Cuts at: 4316 4316 Size: 7012 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6019 809 Size: 507 4703 1802 Fragments arranged by size: 4703 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2132 4459 5348 Size: 54 1425 50 275 18 302 2327 889 Cuts at: 5348 6977 8 Size: 1629 43 Fragments arranged by size: 2327 1629 1425 889 302 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4459 1537 Size: 275 2647 4090 Fragments arranged by size: 4090 2647 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2537 3010 3245 4942 1353 Size: 1184 473 235 1697 3423 Fragments arranged by size: 3423 1697 1184 473 235 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5599 5973 6119 387 Size: 1064 138 4010 374 146 1280 Fragments arranged by size: 4010 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2077 2110 2181 2340 2508 2606 3069 Size: 393 445 33 71 159 168 98 463 Cuts at: 3069 3116 3298 3358 3503 4046 4139 5075 5672 Size: 47 182 60 145 543 93 936 597 Cuts at: 5672 6459 6727 1239 Size: 787 268 1524 Fragments arranged by size: 1524 936 787 597 543 463 445 393 268 182 168 159 145 98 93 71 60 47 33 EcoNI CCTnn'n_nnAGG Cuts at: 3444 3497 3665 3444 Size: 53 168 6791 Fragments arranged by size: 6791 168 53 EcoO109I rG'GnC_Cy Cuts at: 2165 2291 3561 3699 2165 Size: 126 1270 138 5478 Fragments arranged by size: 5478 1270 138 126 EcoRI G'AATT_C Cuts at: 3352 4321 4699 3352 Size: 969 378 5665 Fragments arranged by size: 5665 969 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3916 3916 Size: 7012 FspI TGC'GCA Cuts at: 5518 5518 Size: 7012 HaeII r_GCGC'y Cuts at: 1678 2185 6159 1678 Size: 507 3974 2531 Fragments arranged by size: 3974 2531 507 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2552 2584 2900 2932 2933 Size: 32 135 32 980 32 316 32 1 Cuts at: 2933 2965 5653 5685 5727 5759 1373 Size: 32 2688 32 42 32 2626 Fragments arranged by size: 2688 2626 980 316 135 42 32 32 32 32 32 32 32 1 HincII GTy'rAC Cuts at: 669 1657 1938 4356 4602 669 Size: 988 281 2418 246 3079 Fragments arranged by size: 3079 2418 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7012 HpaI GTT'AAC Cuts at: 4602 4602 Size: 7012 KpnI G_GTAC'C Cuts at: 4337 4337 Size: 7012 MfeI C'AATT_G Cuts at: 2511 4611 2511 Size: 2100 4912 MluI A'CGCG_T Cuts at: 6888 6888 Size: 7012 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2049 6126 6310 1305 Size: 744 4077 184 2007 Fragments arranged by size: 4077 2007 744 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5190 Fragments arranged by size: 5190 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4046 4061 4970 5329 5488 518 Size: 648 2880 15 909 359 159 2042 Fragments arranged by size: 2880 2042 909 648 359 159 15 MspA1I CmG'CkG Cuts at: 1606 2094 2486 3871 3877 4428 5105 6253 6498 Size: 488 392 1385 6 551 677 1148 245 Cuts at: 6498 1606 Size: 2120 Fragments arranged by size: 2120 1385 1148 677 551 488 392 245 6 NaeI GCC'GGC Cuts at: 2132 2132 Size: 7012 NcoI C'CATG_G Cuts at: 513 1065 2022 2680 513 Size: 552 957 658 4845 Fragments arranged by size: 4845 957 658 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7012 NgoMIV G'CCGG_C Cuts at: 2130 2130 Size: 7012 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7012 NotI GC'GGCC_GC Cuts at: 4459 4459 Size: 7012 NspI r_CATG'y Cuts at: 1132 1229 3535 4045 4370 5915 1132 Size: 97 2306 510 325 1545 2229 Fragments arranged by size: 2306 2229 1545 510 325 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7012 PciI A'CATG_T Cuts at: 4041 5911 4041 Size: 1870 5142 PflMI CCAn_nnn'nTGG Cuts at: 1108 3177 1108 Size: 2069 4943 PfoI T'CCnGG_A Cuts at: 2100 2100 Size: 7012 PmeI GTTT'AAAC Cuts at: 4316 4316 Size: 7012 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5081 5113 6594 6626 5081 Size: 32 1481 32 5467 Fragments arranged by size: 5467 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3561 3561 Size: 7012 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7012 PsiI TTA'TAA Cuts at: 4582 4582 Size: 7012 PspOMI G'GGCC_C Cuts at: 2165 2290 2165 Size: 125 6887 PstI C_TGCA'G Cuts at: 830 2429 2725 3876 4364 830 Size: 1599 296 1151 488 3478 Fragments arranged by size: 3478 1599 1151 488 296 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7012 PvuII CAG'CTG Cuts at: 2094 2486 3871 3877 2094 Size: 392 1385 6 5229 Fragments arranged by size: 5229 1385 392 6 RsrII CG'GwC_CG Cuts at: 4219 4219 Size: 7012 SalI G'TCGA_C Cuts at: 1936 4354 1936 Size: 2418 4594 SapI GCTCTTCn'nnn_ Cuts at: 3245 3245 Size: 7012 SbfI CC_TGCA'GG Cuts at: 4364 4364 Size: 7012 ScaI AGT'ACT Cuts at: 4031 5260 6972 4031 Size: 1229 1712 4071 Fragments arranged by size: 4071 1712 1229 SfcI C'TryA_G Cuts at: 826 1045 2425 2721 3291 3733 3872 4297 4360 Size: 219 1380 296 570 442 139 425 63 Cuts at: 4360 5495 6176 6367 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1380 1135 681 570 442 425 296 219 191 139 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7012 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7012 SmaI CCC'GGG Cuts at: 1872 4094 4200 4339 1872 Size: 2222 106 139 4545 Fragments arranged by size: 4545 2222 139 106 SmlI C'TyrA_G Cuts at: 820 1017 1943 2432 2859 3769 4102 5116 6017 Size: 197 926 489 427 910 333 1014 901 Cuts at: 6017 6279 820 Size: 262 1553 Fragments arranged by size: 1553 1014 926 910 901 489 427 333 262 197 SnaBI TAC'GTA Cuts at: 493 4309 4803 493 Size: 3816 494 2702 Fragments arranged by size: 3816 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7012 SphI G_CATG'C Cuts at: 1132 4370 1132 Size: 3238 3774 SspI AAT'ATT Cuts at: 5 52 4936 5 Size: 47 4884 2081 Fragments arranged by size: 4884 2081 47 StuI AGG'CCT Cuts at: 1517 3329 1517 Size: 1812 5200 StyI C'CwwG_G Cuts at: 513 1065 2022 2680 2884 3155 3556 3900 4263 Size: 552 957 658 204 271 401 344 363 Cuts at: 4263 4451 513 Size: 188 3074 Fragments arranged by size: 3074 957 658 552 401 363 344 271 204 188 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2907 5039 5056 5209 5394 5839 325 Size: 1255 1327 2132 17 153 185 445 1498 Fragments arranged by size: 2132 1498 1327 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3144 4029 5258 Size: 274 80 33 51 1443 1167 885 1229 Cuts at: 5258 6970 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1229 1167 885 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6972 7004 6972 Size: 32 6980 Tth111I GACn'n_nGTC Cuts at: 1316 3122 1316 Size: 1806 5206 XbaI T'CTAG_A Cuts at: 2410 4348 2410 Size: 1938 5074 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2389 4045 2389 Size: 1656 5356 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7012 XmaI C'CCGG_G Cuts at: 1870 4092 4198 4337 1870 Size: 2222 106 139 4545 Fragments arranged by size: 4545 2222 139 106 XmnI GAAnn'nnTTC Cuts at: 5141 5141 Size: 7012 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4820 276 Size: 53 83 186 936 3286 2468 Fragments arranged by size: 3286 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI