Restriction map for
pFN21ASDA0218
(Linear) (Six-base) MAPSORT of: KIAA0218.seq Check: 2266 from: 1 to: 2283
KIAA0218.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 485 583 1046 2023 2283
Size: 317 168 98 463 977 260
Fragments arranged by size:
977 463 317 260 168 98
AflII C'TTAA_G
Cuts at: 0 409 1746 2283
Size: 409 1337 537
Fragments arranged by size:
1337 537 409
AflIII A'CryG_T
Cuts at: 0 2018 2283
Size: 2018 265
AleI CACnn'nnGTG
Cuts at: 0 2038 2283
Size: 2038 245
AlwNI CAG_nnn'CTG
Cuts at: 0 730 2283
Size: 730 1553
ApaI G_GGCC'C
Cuts at: 0 146 271 2283
Size: 146 125 2012
Fragments arranged by size:
2012 146 125
ApaLI G'TGCA_C
Cuts at: 0 1803 2283
Size: 1803 480
ApoI r'AATT_y
Cuts at: 0 454 1329 1559 1585 2283
Size: 454 875 230 26 698
Fragments arranged by size:
875 698 454 230 26
AvaI C'yCGr_G
Cuts at: 0 317 623 2069 2175 2283
Size: 317 306 1446 106 108
Fragments arranged by size:
1446 317 306 108 106
BamHI G'GATC_C
Cuts at: 0 340 2283
Size: 340 1943
BanI G'GyrC_C
Cuts at: 0 1751 2283
Size: 1751 532
BanII G_rGCy'C
Cuts at: 0 86 146 217 271 547 860 1997 2283
Size: 86 60 71 54 276 313 1137 286
Fragments arranged by size:
1137 313 286 276 86 71 60 54
BbsI GAAGACnn'nnnn_
Cuts at: 0 323 2283
Size: 323 1960
BbvCI CC'TCA_GC
Cuts at: 0 470 2283
Size: 470 1813
BglI GCCn_nnn'nGGC
Cuts at: 0 625 2065 2283
Size: 625 1440 218
Fragments arranged by size:
1440 625 218
BglII A'GATC_T
Cuts at: 0 1013 1908 2283
Size: 1013 895 375
Fragments arranged by size:
1013 895 375
Bme1580I G_kGCm'C
Cuts at: 0 146 271 1807 1940 2283
Size: 146 125 1536 133 343
Fragments arranged by size:
1536 343 146 133 125
BmgBI CAC'GTC
Cuts at: 0 44 2283
Size: 44 2239
BmrI ACTGGGnnnn_n'
Cuts at: 0 842 2283
Size: 842 1441
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 996 1032 2283
Size: 996 36 1251
Fragments arranged by size:
1251 996 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 87 158 1093 1275 1335 1480 2116
Size: 54 33 71 935 182 60 145 636
Cuts at: 2116 2283
Size: 167
Fragments arranged by size:
935 636 182 167 145 71 60 54
33
Bpu10I CC'TnA_GC
Cuts at: 0 470 2283
Size: 470 1813
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 821 2100 2283
Size: 821 1279 183
Fragments arranged by size:
1279 821 183
BsaI GGTCTCn'nnnn_
Cuts at: 0 530 2283
Size: 530 1753
BsaBI GATnn'nnATC
Cuts at: 0 1907 2283
Size: 1907 376
BsaHI Gr'CG_yC
Cuts at: 0 4 2283
Size: 4 2279
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 226 256 529 559 1555 1585 1636 1666
Size: 226 30 273 30 996 30 51 30
Cuts at: 1666 2106 2136 2283
Size: 440 30 147
Fragments arranged by size:
996 440 273 226 147 51 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 228 231 234 406 790 813 868 1024
Size: 228 3 3 172 384 23 55 156
Cuts at: 1024 1103 1414 1489 1660 2043 2283
Size: 79 311 75 171 383 240
Fragments arranged by size:
384 383 311 240 228 172 171 156
79 75 55 23 3 3
BseYI C'CCAG_C
Cuts at: 0 145 652 2283
Size: 145 507 1631
Fragments arranged by size:
1631 507 145
BsiHKAI G_wGCw'C
Cuts at: 0 977 1807 2283
Size: 977 830 476
Fragments arranged by size:
977 830 476
Bsp1286I G_dGCh'C
Cuts at: 0 86 146 217 271 547 860 977 1807
Size: 86 60 71 54 276 313 117 830
Cuts at: 1807 1940 1997 2283
Size: 133 57 286
Fragments arranged by size:
830 313 286 276 133 117 86 71
60 57 54
BspHI T'CATG_A
Cuts at: 0 1922 2283
Size: 1922 361
BsrBI CCG'CTC
Cuts at: 0 12 2283
Size: 12 2271
BsrDI GCAATG_nn'
Cuts at: 0 1962 2283
Size: 1962 321
BsrFI r'CCGG_y
Cuts at: 0 107 1972 2283
Size: 107 1865 311
Fragments arranged by size:
1865 311 107
BsrGI T'GTAC_A
Cuts at: 0 1121 2283
Size: 1121 1162
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1299 2283
Size: 1299 984
BstYI r'GATC_y
Cuts at: 0 340 1013 1662 1908 1952 2087 2283
Size: 340 673 649 246 44 135 196
Fragments arranged by size:
673 649 340 246 196 135 44
Bsu36I CC'TnA_GG
Cuts at: 0 1580 1757 2283
Size: 1580 177 526
Fragments arranged by size:
1580 526 177
BtgI C'CryG_G
Cuts at: 0 657 2283
Size: 657 1626
BtsI GCAGTG_nn'
Cuts at: 0 728 1887 2045 2283
Size: 728 1159 158 238
Fragments arranged by size:
1159 728 238 158
ClaI AT'CG_AT
Cuts at: 0 592 2283
Size: 592 1691
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 245 280 2283
Size: 245 35 2003
Fragments arranged by size:
2003 245 35
EaeI y'GGCC_r
Cuts at: 0 109 2283
Size: 109 2174
EarI CTCTTCn'nnn_
Cuts at: 0 514 987 1222 2283
Size: 514 473 235 1061
Fragments arranged by size:
1061 514 473 235
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 87 158 317 485 583 1046 1093
Size: 54 33 71 159 168 98 463 47
Cuts at: 1093 1275 1335 1480 2023 2116 2283
Size: 182 60 145 543 93 167
Fragments arranged by size:
543 463 182 168 167 159 145 98
93 71 60 54 47 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1421 1474 1642 2283
Size: 1421 53 168 641
Fragments arranged by size:
1421 641 168 53
EcoO109I rG'GnC_Cy
Cuts at: 0 142 268 1538 1676 2283
Size: 142 126 1270 138 607
Fragments arranged by size:
1270 607 142 138 126
EcoRI G'AATT_C
Cuts at: 0 1329 2283
Size: 1329 954
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1893 2283
Size: 1893 390
HaeII r_GCGC'y
Cuts at: 0 162 2283
Size: 162 2121
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 529 561 877 909 910 942 2283
Size: 529 32 316 32 1 32 1341
Fragments arranged by size:
1341 529 316 32 32 32 1
MfeI C'AATT_G
Cuts at: 0 488 2283
Size: 488 1795
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 2283
Size: 26 2257
MslI CAynn'nnrTG
Cuts at: 0 2023 2038 2283
Size: 2023 15 245
Fragments arranged by size:
2023 245 15
MspA1I CmG'CkG
Cuts at: 0 71 463 1848 1854 2283
Size: 71 392 1385 6 429
Fragments arranged by size:
1385 429 392 71 6
NaeI GCC'GGC
Cuts at: 0 109 2283
Size: 109 2174
NcoI C'CATG_G
Cuts at: 0 657 2283
Size: 657 1626
NgoMIV G'CCGG_C
Cuts at: 0 107 2283
Size: 107 2176
NspI r_CATG'y
Cuts at: 0 1512 2022 2283
Size: 1512 510 261
Fragments arranged by size:
1512 510 261
PciI A'CATG_T
Cuts at: 0 2018 2283
Size: 2018 265
PflMI CCAn_nnn'nTGG
Cuts at: 0 1154 2283
Size: 1154 1129
PfoI T'CCnGG_A
Cuts at: 0 77 2283
Size: 77 2206
PpuMI rG'GwC_Cy
Cuts at: 0 1538 2283
Size: 1538 745
PspOMI G'GGCC_C
Cuts at: 0 142 267 2283
Size: 142 125 2016
Fragments arranged by size:
2016 142 125
PstI C_TGCA'G
Cuts at: 0 406 702 1853 2283
Size: 406 296 1151 430
Fragments arranged by size:
1151 430 406 296
PvuII CAG'CTG
Cuts at: 0 71 463 1848 1854 2283
Size: 71 392 1385 6 429
Fragments arranged by size:
1385 429 392 71 6
RsrII CG'GwC_CG
Cuts at: 0 2196 2283
Size: 2196 87
SapI GCTCTTCn'nnn_
Cuts at: 0 1222 2283
Size: 1222 1061
ScaI AGT'ACT
Cuts at: 0 2008 2283
Size: 2008 275
SfcI C'TryA_G
Cuts at: 0 402 698 1268 1710 1849 2274 2283
Size: 402 296 570 442 139 425 9
Fragments arranged by size:
570 442 425 402 296 139 9
SmaI CCC'GGG
Cuts at: 0 2071 2177 2283
Size: 2071 106 106
Fragments arranged by size:
2071 106 106
SmlI C'TyrA_G
Cuts at: 0 409 836 1746 2079 2283
Size: 409 427 910 333 204
Fragments arranged by size:
910 427 409 333 204
StuI AGG'CCT
Cuts at: 0 1306 2283
Size: 1306 977
StyI C'CwwG_G
Cuts at: 0 657 861 1132 1533 1877 2240 2283
Size: 657 204 271 401 344 363 43
Fragments arranged by size:
657 401 363 344 271 204 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 884 2283
Size: 884 1399
TatI w'GTAC_w
Cuts at: 0 1121 2006 2283
Size: 1121 885 277
Fragments arranged by size:
1121 885 277
Tth111I GACn'n_nGTC
Cuts at: 0 1099 2283
Size: 1099 1184
XbaI T'CTAG_A
Cuts at: 0 387 2283
Size: 387 1896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 366 2022 2283
Size: 366 1656 261
Fragments arranged by size:
1656 366 261
XmaI C'CCGG_G
Cuts at: 0 2069 2175 2283
Size: 2069 106 108
Fragments arranged by size:
2069 108 106
Enzymes that do cut:
AcuI AflII AflIII AleI AlwNI ApaI ApaLI ApoI
AvaI BamHI BanI BanII BbsI BbvCI BglI BglII
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspHI
BsrBI BsrDI BsrFI BsrGI BstXI BstYI Bsu36I BtgI
BtsI ClaI CspCI EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I MfeI MmeI MslI MspA1I
NaeI NcoI NgoMIV NspI PciI PflMI PfoI PpuMI
PspOMI PstI PvuII RsrII SapI ScaI SfcI SmaI
SmlI StuI StyI TaqII TatI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AlfI AloI AscI AseI AsiSI AvrII BaeI BbeI
BcgI BciVI BclI BfrBI BlpI BmtI BsaAI BsaWI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspMI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtgZI DraI DraIII
DrdI EagI EciI EcoICRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MluI MscI NarI
NdeI NheI NotI NruI NsiI PacI PasI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI SacI
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI
XhoI XmnI ZraI