Restriction map for
pFN21ASDA0215
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII AccII AclI AcyI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AspEI AspI
AviII AvrII BbeI BbrPI BclI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaHI BsiEI BsiWI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CelII CfoI ClaI
CpoI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI KasI Ksp632I
KspI MfeI MluI MunI MvnI
NaeI NarI NdeI NgoMIV NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 243 GGTACC G^GTACC
AccI 2243 GTAGAC GT^[AC][GT]AC
AccIII 131 TCCGGA T^CCGGA
AcsI 95 AAATTC [GA]^AATT[CT]
AocI 1744 CCTCAGG CC^T[ACGT]AGG
ApoI 95 AAATTC [GA]^AATT[CT]
AseI 178 ATTAAT AT^TAAT
AsnI 178 ATTAAT AT^TAAT
Asp700I 1387 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 243 GGTACC G^GTACC
AvaI 2046 CTCGAG C^[CT]CG[GA]G
BalI 1817 TGGCCA TGG^CCA
BamHI 845 GGATCC G^GATCC
BbvCI 121 GCTGAGG CCTCAGC(-5/-2)
BcgI 1447 CGAAATCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1776 GTATCC GTATCC(6/5)
BcnI 326 CCCGG CC^[GC]GG
BglI 2003 GCCTGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 490 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 131 TCCGGA [AT]^CCGG[AT]
BseAI 131 TCCGGA T^CCGGA
BsgI 1372 GTGCAG GTGCAG(16/14)
BsmBI 1058 GAGACG CGTCTC(1/5)
BsmI 1391 GCATTC GAATGC(1/-1)
BsoBI 2046 CTCGAG C^[CT]CG[GA]G
BspEI 131 TCCGGA T^CCGGA
BsrFI 617 GCCGGT [GA]^CCGG[CT]
BstAPI 935 GCAACTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1997 CCAATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1744 CCTCAGG CC^T[ACGT]AGG
Cfr10I 617 GCCGGT [GA]^CCGG[CT]
DraI 2146 TTTAAA TTT^AAA
Eco81I 1744 CCTCAGG CC^T[ACGT]AGG
EcoNI 1047 CCTAGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1417 ATGCAT ATGCA^T
FauI 1185 GCGGG CCCGC(4/6)
HindIII 1195 AAGCTT A^AGCTT
KpnI 243 GGTACC GGTAC^C
MamI 490 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1817 TGGCCA TGG^CCA
MroI 131 TCCGGA T^CCGGA
MscI 1817 TGGCCA TGG^CCA
NciI 326 CCCGG CC^[GC]GG
NheI 1272 GCTAGC G^CTAGC
NsiI 1417 ATGCAT ATGCA^T
NspI 1733 ACATGC [GA]CATG^[CT]
PaeR7I 2046 CTCGAG C^TCGAG
PshAI 40 GACGAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 178 ATTAAT AT^TAAT
PstI 2264 CTGCAG CTGCA^G
SacI 2273 GAGCTC GAGCT^C
XbaI 2407 TCTAGA T^CTAGA
XhoI 2046 CTCGAG C^TCGAG
XmnI 1387 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
BanI 243 GGTACC G^G[CT][GA]CC
1740 GGTGCC
BglII 896 AGATCT A^GATCT
1514 AGATCT
BsaI 2152 GAGACC GGTCTC(1/5)
620 GGTCTC
BseRI 1210 GAGGAG GAGGAG(10/8)
1213 GAGGAG
BsmFI 811 GGGAC GGGAC(10/14)
709 GTCCC
BspMI 117 ACCTGC ACCTGC(4/8)
1737 GCAGGT
DsaI 1938 CCATGG C^C[GA][CT]GG
2421 CCATGG
EaeI 1247 TGGCCG [CT]^GGCC[GA]
1817 TGGCCA
NcoI 1938 CCATGG C^CATGG
2421 CCATGG
SfcI 2123 CTGTAG C^T[GA][CT]AG
2264 CTGCAG
StuI 13 AGGCCT AGG^CCT
686 AGGCCT
TaqI 1401 TCGA T^CGA
2047 TCGA
TthHB8I 1401 TCGA T^CGA
2047 TCGA
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XcmI XhoII