(Circular) (Six-base) MAPSORT of: pFN21ASDA0215.seq Check: 3410 from: 1 to: 7198 pFN21ASDA0215 7198 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3751 3751 Size: 7198 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5008 278 Size: 53 83 186 936 3472 2468 Fragments arranged by size: 3472 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4267 4541 1937 Size: 2330 274 4594 Fragments arranged by size: 4594 2330 274 Acc65I G'GTAC_C Cuts at: 2266 4519 2266 Size: 2253 4945 AclI AA'CG_TT Cuts at: 1563 5325 5698 1563 Size: 3762 373 3063 Fragments arranged by size: 3762 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2450 2837 3350 3527 3932 5261 6645 6913 Size: 818 387 513 177 405 1329 1384 268 Cuts at: 6913 1632 Size: 1917 Fragments arranged by size: 1917 1384 1329 818 513 405 387 268 177 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7001 AflIII A'CryG_T Cuts at: 1203 6097 7074 1203 Size: 4894 977 1327 Fragments arranged by size: 4894 1327 977 AgeI A'CCGG_T Cuts at: 6029 6029 Size: 7198 AhdI GACnn_n'nnGTC Cuts at: 5927 5927 Size: 7198 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7198 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2443 1184 Size: 1259 5939 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3224 3256 4085 4117 3224 Size: 32 829 32 6305 Fragments arranged by size: 6305 829 32 32 AlwNI CAG_nnn'CTG Cuts at: 2390 2423 3457 3928 4941 6513 2390 Size: 33 1034 471 1013 1572 3075 Fragments arranged by size: 3075 1572 1034 1013 471 33 ApaLI G'TGCA_C Cuts at: 5255 6411 5255 Size: 1156 6042 ApoI r'AATT_y Cuts at: 1493 2118 4507 4733 4885 6994 1493 Size: 625 2389 226 152 2109 1697 Fragments arranged by size: 2389 2109 1697 625 226 152 AseI AT'TA_AT Cuts at: 160 1032 2202 5752 160 Size: 872 1170 3550 1606 Fragments arranged by size: 3550 1606 1170 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7198 AvaI C'yCGr_G Cuts at: 1870 1943 4069 4523 1870 Size: 73 2126 454 4545 Fragments arranged by size: 4545 2126 454 73 BamHI G'GATC_C Cuts at: 2868 4528 4895 2868 Size: 1660 367 5171 Fragments arranged by size: 5171 1660 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2266 3763 4519 5974 618 Size: 325 217 627 479 1497 756 1455 1842 Fragments arranged by size: 1842 1497 1455 756 627 479 325 217 BanII G_rGCy'C Cuts at: 1391 2815 2848 4247 4300 4517 1391 Size: 1424 33 1399 53 217 4072 Fragments arranged by size: 4072 1424 1399 217 53 33 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2487 2825 2981 3068 3681 3963 928 Size: 971 588 338 156 87 613 282 4163 Fragments arranged by size: 4163 971 613 588 338 282 156 87 BbvCI CC'TCA_GC Cuts at: 2145 2145 Size: 7198 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3459 3493 5389 5423 1903 Size: 34 1522 34 1896 34 3678 Fragments arranged by size: 3678 1896 1522 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3810 5090 6300 3810 Size: 1280 1210 4708 Fragments arranged by size: 4708 1280 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7198 BfrBI ATG'CAT Cuts at: 3442 3442 Size: 7198 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4032 5809 136 Size: 107 122 71 631 605 2360 1777 1525 Fragments arranged by size: 2360 1777 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2919 3537 7193 2919 Size: 618 3656 2924 Fragments arranged by size: 3656 2924 618 BlpI GC'TnA_GC Cuts at: 4615 4615 Size: 7198 Bme1580I G_kGCm'C Cuts at: 1790 2863 3214 5259 6415 1790 Size: 1073 351 2045 1156 2573 Fragments arranged by size: 2573 2045 1156 1073 351 BmgBI CAC'GTC Cuts at: 1318 3028 1318 Size: 1710 5488 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2931 3938 4929 Size: 416 53 83 399 24 1514 1007 991 Cuts at: 4929 5887 442 Size: 958 1753 Fragments arranged by size: 1753 1514 1007 991 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3299 1056 Size: 2243 4955 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2353 2631 3251 3479 4049 4196 5858 1239 Size: 1114 278 620 228 570 147 1662 2579 Fragments arranged by size: 2579 1662 1114 620 570 278 228 147 Bpu10I CC'TnA_GC Cuts at: 2145 2145 Size: 7198 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2711 3095 5323 6188 6486 2711 Size: 384 2228 865 298 3423 Fragments arranged by size: 3423 2228 865 384 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2649 4169 5861 882 Size: 617 1150 1520 1692 2219 Fragments arranged by size: 2219 1692 1520 1150 617 BsaAI yAC'GTr Cuts at: 493 1204 4495 4989 493 Size: 711 3291 494 2702 Fragments arranged by size: 3291 2702 711 494 BsaBI GATnn'nnATC Cuts at: 2517 7192 2517 Size: 4675 2523 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5005 5387 6022 275 Size: 53 83 186 936 3472 382 635 1451 Fragments arranged by size: 3472 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2154 5631 6029 6303 6450 6861 2154 Size: 3477 398 274 147 411 2491 Fragments arranged by size: 3477 2491 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2250 2280 3072 3102 3224 Size: 30 165 30 847 30 792 30 122 Cuts at: 3224 3254 4085 4115 1178 Size: 30 831 30 4261 Fragments arranged by size: 4261 847 831 792 165 122 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3248 3251 1186 Size: 2062 3 5133 Fragments arranged by size: 5133 2062 3 BseYI C'CCAG_C Cuts at: 3280 6401 6897 3280 Size: 3121 496 3581 Fragments arranged by size: 3581 3121 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3416 1163 Size: 573 1680 4945 Fragments arranged by size: 4945 1680 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4648 5409 6437 1540 Size: 275 186 2647 761 1028 2301 Fragments arranged by size: 2647 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2999 3048 4300 5259 5344 6415 2999 Size: 49 1252 959 85 1071 3782 Fragments arranged by size: 3782 1252 1071 959 85 49 BsmI GAATG_Cn' Cuts at: 3414 4709 4802 3414 Size: 1295 93 5810 Fragments arranged by size: 5810 1295 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3075 3075 Size: 7198 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2815 2848 2863 2999 3048 3214 4247 Size: 399 1025 33 15 136 49 166 1033 Cuts at: 4247 4300 4517 5259 5344 6415 1391 Size: 53 217 742 85 1071 2174 Fragments arranged by size: 2174 1071 1033 1025 742 399 217 166 136 85 53 49 33 15 BspEI T'CCGG_A Cuts at: 2154 6861 2154 Size: 4707 2491 BspHI T'CATG_A Cuts at: 5087 6817 5087 Size: 1730 5468 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2149 3751 4553 844 Size: 1305 1602 802 3489 Fragments arranged by size: 3489 1602 1305 802 BsrBI CCG'CTC Cuts at: 1394 1823 5085 1394 Size: 429 3262 3507 Fragments arranged by size: 3507 3262 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2554 2789 2883 5693 5867 65 Size: 1183 1306 235 94 2810 174 1396 Fragments arranged by size: 2810 1396 1306 1183 235 174 94 BsrFI r'CCGG_y Cuts at: 1700 1810 2640 5842 6029 1700 Size: 110 830 3202 187 2869 Fragments arranged by size: 3202 2869 830 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7198 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7198 BssSI C'ACGA_G Cuts at: 5258 6270 5258 Size: 1012 6186 BstAPI GCAn_nnn'nTGC Cuts at: 794 2964 794 Size: 2170 5028 BstBI TT'CG_AA Cuts at: 2007 6002 2007 Size: 3995 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7198 BstXI CCAn_nnnn'nTGG Cuts at: 4027 4027 Size: 7198 BstYI r'GATC_y Cuts at: 1972 2868 2919 3537 4528 4895 5280 5297 6749 Size: 896 51 618 991 367 385 17 1452 Cuts at: 6749 6835 6847 7193 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 991 896 618 385 367 346 86 51 17 12 Bsu36I CC'TnA_GG Cuts at: 3768 3768 Size: 7198 BtgI C'CryG_G Cuts at: 513 1065 3961 4444 513 Size: 552 2896 483 3267 Fragments arranged by size: 3267 2896 552 483 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6459 Fragments arranged by size: 6459 683 56 BtsI GCAGTG_nn' Cuts at: 2056 2362 3015 4714 5508 5528 2056 Size: 306 653 1699 794 20 3726 Fragments arranged by size: 3726 1699 794 653 306 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7198 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7163 DraI TTT'AAA Cuts at: 4171 4502 4171 Size: 331 6867 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6205 809 Size: 507 4889 1802 Fragments arranged by size: 4889 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3270 3840 4645 Size: 54 1425 50 275 18 1440 570 805 Cuts at: 4645 5534 7163 8 Size: 889 1629 43 Fragments arranged by size: 1629 1440 1425 889 805 570 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4645 1537 Size: 275 2833 4090 Fragments arranged by size: 4090 2833 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5128 1353 Size: 3775 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5785 6159 6305 387 Size: 1064 138 4196 374 146 1280 Fragments arranged by size: 4196 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4298 4298 Size: 7198 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2353 2450 2631 2837 3251 3350 3479 Size: 393 721 97 181 206 414 99 129 Cuts at: 3479 3527 3932 4049 4196 5261 5858 6645 6913 Size: 48 405 117 147 1065 597 787 268 Cuts at: 6913 1239 Size: 1524 Fragments arranged by size: 1524 1065 787 721 597 414 405 393 268 206 181 147 129 117 99 97 48 EcoNI CCTnn'n_nnAGG Cuts at: 3074 3074 Size: 7198 EcoO109I rG'GnC_Cy Cuts at: 2161 2731 3454 4164 4207 2161 Size: 570 723 710 43 5152 Fragments arranged by size: 5152 723 710 570 43 EcoRI G'AATT_C Cuts at: 4507 4885 4507 Size: 378 6820 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3575 3575 Size: 7198 FspI TGC'GCA Cuts at: 5704 5704 Size: 7198 HaeII r_GCGC'y Cuts at: 1678 6345 1678 Size: 4667 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3716 3748 4260 4292 5839 Size: 32 135 32 2144 32 512 32 1547 Cuts at: 5839 5871 5913 5945 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2144 1547 512 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4542 4788 669 Size: 988 281 2604 246 3079 Fragments arranged by size: 3079 2604 988 281 246 HindIII A'AGCT_T Cuts at: 748 3218 748 Size: 2470 4728 HpaI GTT'AAC Cuts at: 4788 4788 Size: 7198 KpnI G_GTAC'C Cuts at: 2270 4523 2270 Size: 2253 4945 MfeI C'AATT_G Cuts at: 4797 4797 Size: 7198 MluI A'CGCG_T Cuts at: 7074 7074 Size: 7198 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2368 6312 6496 1305 Size: 1063 3944 184 2007 Fragments arranged by size: 3944 2007 1063 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3842 10 Size: 54 1425 343 2010 3366 Fragments arranged by size: 3366 2010 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2559 3425 3761 5156 5515 5674 518 Size: 648 1393 866 336 1395 359 159 2042 Fragments arranged by size: 2042 1395 1393 866 648 359 336 159 MspA1I CmG'CkG Cuts at: 1606 2745 2856 4038 4614 5291 6439 6684 1606 Size: 1139 111 1182 576 677 1148 245 2120 Fragments arranged by size: 2120 1182 1148 1139 677 576 245 111 NcoI C'CATG_G Cuts at: 513 1065 3961 4444 513 Size: 552 2896 483 3267 Fragments arranged by size: 3267 2896 552 483 NdeI CA'TA_TG Cuts at: 387 387 Size: 7198 NheI G'CTAG_C Cuts at: 1052 3295 1052 Size: 2243 4955 NotI GC'GGCC_GC Cuts at: 4645 4645 Size: 7198 NsiI A_TGCA'T Cuts at: 3444 3444 Size: 7198 NspI r_CATG'y Cuts at: 1132 1229 3760 4556 6101 1132 Size: 97 2531 796 1545 2229 Fragments arranged by size: 2531 2229 1545 796 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7198 PciI A'CATG_T Cuts at: 6097 6097 Size: 7198 PflMI CCAn_nnn'nTGG Cuts at: 1108 2943 3457 4188 1108 Size: 1835 514 731 4118 Fragments arranged by size: 4118 1835 731 514 PfoI T'CCnGG_A Cuts at: 2544 2544 Size: 7198 PmeI GTTT'AAAC Cuts at: 4502 4502 Size: 7198 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3224 3256 4085 4117 5267 5299 6780 6812 3224 Size: 32 829 32 1150 32 1481 32 3610 Fragments arranged by size: 3610 1481 1150 829 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2161 2731 3454 4164 4207 2161 Size: 570 723 710 43 5152 Fragments arranged by size: 5152 723 710 570 43 PshAI GACnn'nnGTC Cuts at: 1613 2067 1613 Size: 454 6744 PsiI TTA'TAA Cuts at: 4768 4768 Size: 7198 PspXI vC'TCGA_Gb Cuts at: 4069 4069 Size: 7198 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2316 2348 2316 Size: 32 7166 PstI C_TGCA'G Cuts at: 830 4291 4550 830 Size: 3461 259 3478 Fragments arranged by size: 3478 3461 259 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7198 PvuII CAG'CTG Cuts at: 2745 2856 4038 2745 Size: 111 1182 5905 Fragments arranged by size: 5905 1182 111 SacI G_AGCT'C Cuts at: 4300 4300 Size: 7198 SalI G'TCGA_C Cuts at: 1936 4540 1936 Size: 2604 4594 SbfI CC_TGCA'GG Cuts at: 4550 4550 Size: 7198 ScaI AGT'ACT Cuts at: 5446 7158 5446 Size: 1712 5486 SfcI C'TryA_G Cuts at: 826 1045 4146 4287 4546 5681 6362 6553 826 Size: 219 3101 141 259 1135 681 191 1471 Fragments arranged by size: 3101 1471 1135 681 259 219 191 141 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7198 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7198 SmaI CCC'GGG Cuts at: 1872 4525 1872 Size: 2653 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2726 3110 4069 5302 6203 6465 Size: 197 926 783 384 959 1233 901 262 Cuts at: 6465 820 Size: 1553 Fragments arranged by size: 1553 1233 959 926 901 783 384 262 197 SnaBI TAC'GTA Cuts at: 493 4495 4989 493 Size: 4002 494 2702 Fragments arranged by size: 4002 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7198 SphI G_CATG'C Cuts at: 1132 4556 1132 Size: 3424 3774 SspI AAT'ATT Cuts at: 5 52 5122 5 Size: 47 5070 2081 Fragments arranged by size: 5070 2081 47 StuI AGG'CCT Cuts at: 1517 2038 2711 1517 Size: 521 673 6004 Fragments arranged by size: 6004 673 521 StyI C'CwwG_G Cuts at: 513 1065 3249 3961 4248 4444 4637 513 Size: 552 2184 712 287 196 193 3074 Fragments arranged by size: 3074 2184 712 552 287 196 193 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2950 4070 4180 5225 5242 5395 5580 Size: 1255 1370 1120 110 1045 17 153 185 Cuts at: 5580 6025 325 Size: 445 1498 Fragments arranged by size: 1498 1370 1255 1120 1045 445 185 153 110 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5444 7156 96 Size: 274 80 33 51 1443 3467 1712 138 Fragments arranged by size: 3467 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3072 3104 4199 4231 7158 7190 3072 Size: 32 1095 32 2927 32 3080 Fragments arranged by size: 3080 2927 1095 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7198 XbaI T'CTAG_A Cuts at: 4430 4534 4430 Size: 104 7094 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2742 2940 3298 2742 Size: 198 358 6642 Fragments arranged by size: 6642 358 198 XhoI C'TCGA_G Cuts at: 1943 4069 1943 Size: 2126 5072 XmaI C'CCGG_G Cuts at: 1870 4523 1870 Size: 2653 4545 XmnI GAAnn'nnTTC Cuts at: 3414 5327 3414 Size: 1913 5285 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5006 276 Size: 53 83 186 936 3472 2468 Fragments arranged by size: 3472 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI