Restriction map for
pFN21ASDA0215
(Linear) (Six-base) MAPSORT of: KIAA0215.seq Check: 2954 from: 1 to: 2469
KIAA0215.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1728 2469
Size: 1728 741
AccI GT'mk_AC
Cuts at: 0 2244 2469
Size: 2244 225
Acc65I G'GTAC_C
Cuts at: 0 243 2469
Size: 243 2226
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 814 1327 1504 1909 2469
Size: 427 387 513 177 405 560
Fragments arranged by size:
560 513 427 405 387 177
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 420 2469
Size: 420 2049
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1201 1233 2062 2094 2469
Size: 1201 32 829 32 375
Fragments arranged by size:
1201 829 375 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 367 400 1434 1905 2469
Size: 367 33 1034 471 564
Fragments arranged by size:
1034 564 471 367 33
ApoI r'AATT_y
Cuts at: 0 95 2469
Size: 95 2374
AseI AT'TA_AT
Cuts at: 0 179 2469
Size: 179 2290
AvaI C'yCGr_G
Cuts at: 0 2046 2469
Size: 2046 423
BamHI G'GATC_C
Cuts at: 0 845 2469
Size: 845 1624
BanI G'GyrC_C
Cuts at: 0 243 1740 2469
Size: 243 1497 729
Fragments arranged by size:
1497 729 243
BanII G_rGCy'C
Cuts at: 0 792 825 2224 2277 2469
Size: 792 33 1399 53 192
Fragments arranged by size:
1399 792 192 53 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 464 802 958 1045 1658 1940 2469
Size: 464 338 156 87 613 282 529
Fragments arranged by size:
613 529 464 338 282 156 87
BbvCI CC'TCA_GC
Cuts at: 0 122 2469
Size: 122 2347
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1436 1470 2469
Size: 1436 34 999
Fragments arranged by size:
1436 999 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1787 2469
Size: 1787 682
BfrBI ATG'CAT
Cuts at: 0 1419 2469
Size: 1419 1050
BglI GCCn_nnn'nGGC
Cuts at: 0 2009 2469
Size: 2009 460
BglII A'GATC_T
Cuts at: 0 896 1514 2469
Size: 896 618 955
Fragments arranged by size:
955 896 618
Bme1580I G_kGCm'C
Cuts at: 0 840 1191 2469
Size: 840 351 1278
Fragments arranged by size:
1278 840 351
BmgBI CAC'GTC
Cuts at: 0 1005 2469
Size: 1005 1464
BmrI ACTGGGnnnn_n'
Cuts at: 0 908 1915 2469
Size: 908 1007 554
Fragments arranged by size:
1007 908 554
BmtI G_CTAG'C
Cuts at: 0 1276 2469
Size: 1276 1193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 608 1228 1456 2026 2173 2469
Size: 330 278 620 228 570 147 296
Fragments arranged by size:
620 570 330 296 278 228 147
Bpu10I CC'TnA_GC
Cuts at: 0 122 2469
Size: 122 2347
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 1072 2469
Size: 688 384 1397
Fragments arranged by size:
1397 688 384
BsaI GGTCTCn'nnnn_
Cuts at: 0 626 2146 2469
Size: 626 1520 323
Fragments arranged by size:
1520 626 323
BsaBI GATnn'nnATC
Cuts at: 0 494 2469
Size: 494 1975
BsaWI w'CCGG_w
Cuts at: 0 131 2469
Size: 131 2338
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 227 257 1049 1079 1201 1231 2062 2092
Size: 227 30 792 30 122 30 831 30
Cuts at: 2092 2469
Size: 377
Fragments arranged by size:
831 792 377 227 122 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1225 1228 2469
Size: 1225 3 1241
Fragments arranged by size:
1241 1225 3
BseYI C'CCAG_C
Cuts at: 0 1257 2469
Size: 1257 1212
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1393 2469
Size: 1393 1076
BsiHKAI G_wGCw'C
Cuts at: 0 976 1025 2277 2469
Size: 976 49 1252 192
Fragments arranged by size:
1252 976 192 49
BsmI GAATG_Cn'
Cuts at: 0 1391 2469
Size: 1391 1078
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1052 2469
Size: 1052 1417
Bsp1286I G_dGCh'C
Cuts at: 0 792 825 840 976 1025 1191 2224 2277
Size: 792 33 15 136 49 166 1033 53
Cuts at: 2277 2469
Size: 192
Fragments arranged by size:
1033 792 192 166 136 53 49 33
15
BspEI T'CCGG_A
Cuts at: 0 131 2469
Size: 131 2338
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 126 1728 2469
Size: 126 1602 741
Fragments arranged by size:
1602 741 126
BsrDI GCAATG_nn'
Cuts at: 0 531 766 860 2469
Size: 531 235 94 1609
Fragments arranged by size:
1609 531 235 94
BsrFI r'CCGG_y
Cuts at: 0 617 2469
Size: 617 1852
BstAPI GCAn_nnn'nTGC
Cuts at: 0 941 2469
Size: 941 1528
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2004 2469
Size: 2004 465
BstYI r'GATC_y
Cuts at: 0 845 896 1514 2469
Size: 845 51 618 955
Fragments arranged by size:
955 845 618 51
Bsu36I CC'TnA_GG
Cuts at: 0 1745 2469
Size: 1745 724
BtgI C'CryG_G
Cuts at: 0 1938 2421 2469
Size: 1938 483 48
Fragments arranged by size:
1938 483 48
BtsI GCAGTG_nn'
Cuts at: 0 33 339 992 2469
Size: 33 306 653 1477
Fragments arranged by size:
1477 653 306 33
DraI TTT'AAA
Cuts at: 0 2148 2469
Size: 2148 321
EaeI y'GGCC_r
Cuts at: 0 1247 1817 2469
Size: 1247 570 652
Fragments arranged by size:
1247 652 570
EcoICRI GAG'CTC
Cuts at: 0 2275 2469
Size: 2275 194
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 427 608 814 1228 1327 1456 1504
Size: 330 97 181 206 414 99 129 48
Cuts at: 1504 1909 2026 2173 2469
Size: 405 117 147 296
Fragments arranged by size:
414 405 330 296 206 181 147 129
117 99 97 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1051 2469
Size: 1051 1418
EcoO109I rG'GnC_Cy
Cuts at: 0 138 708 1431 2141 2184 2469
Size: 138 570 723 710 43 285
Fragments arranged by size:
723 710 570 285 138 43
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1552 2469
Size: 1552 917
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1693 1725 2237 2269 2469
Size: 1693 32 512 32 200
Fragments arranged by size:
1693 512 200 32 32
HindIII A'AGCT_T
Cuts at: 0 1195 2469
Size: 1195 1274
KpnI G_GTAC'C
Cuts at: 0 247 2469
Size: 247 2222
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 2469
Size: 345 2124
MscI TGG'CCA
Cuts at: 0 1819 2469
Size: 1819 650
MslI CAynn'nnrTG
Cuts at: 0 536 1402 1738 2469
Size: 536 866 336 731
Fragments arranged by size:
866 731 536 336
MspA1I CmG'CkG
Cuts at: 0 722 833 2015 2469
Size: 722 111 1182 454
Fragments arranged by size:
1182 722 454 111
NcoI C'CATG_G
Cuts at: 0 1938 2421 2469
Size: 1938 483 48
Fragments arranged by size:
1938 483 48
NheI G'CTAG_C
Cuts at: 0 1272 2469
Size: 1272 1197
NsiI A_TGCA'T
Cuts at: 0 1421 2469
Size: 1421 1048
NspI r_CATG'y
Cuts at: 0 1737 2469
Size: 1737 732
PflMI CCAn_nnn'nTGG
Cuts at: 0 920 1434 2165 2469
Size: 920 514 731 304
Fragments arranged by size:
920 731 514 304
PfoI T'CCnGG_A
Cuts at: 0 521 2469
Size: 521 1948
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1201 1233 2062 2094 2469
Size: 1201 32 829 32 375
Fragments arranged by size:
1201 829 375 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 138 708 1431 2141 2184 2469
Size: 138 570 723 710 43 285
Fragments arranged by size:
723 710 570 285 138 43
PshAI GACnn'nnGTC
Cuts at: 0 44 2469
Size: 44 2425
PspXI vC'TCGA_Gb
Cuts at: 0 2046 2469
Size: 2046 423
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 293 325 2469
Size: 293 32 2144
Fragments arranged by size:
2144 293 32
PstI C_TGCA'G
Cuts at: 0 2268 2469
Size: 2268 201
PvuII CAG'CTG
Cuts at: 0 722 833 2015 2469
Size: 722 111 1182 454
Fragments arranged by size:
1182 722 454 111
SacI G_AGCT'C
Cuts at: 0 2277 2469
Size: 2277 192
SfcI C'TryA_G
Cuts at: 0 2123 2264 2469
Size: 2123 141 205
Fragments arranged by size:
2123 205 141
SmlI C'TyrA_G
Cuts at: 0 703 1087 2046 2469
Size: 703 384 959 423
Fragments arranged by size:
959 703 423 384
StuI AGG'CCT
Cuts at: 0 15 688 2469
Size: 15 673 1781
Fragments arranged by size:
1781 673 15
StyI C'CwwG_G
Cuts at: 0 1226 1938 2225 2421 2469
Size: 1226 712 287 196 48
Fragments arranged by size:
1226 712 287 196 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 927 2047 2157 2469
Size: 927 1120 110 312
Fragments arranged by size:
1120 927 312 110
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1049 1081 2176 2208 2469
Size: 1049 32 1095 32 261
Fragments arranged by size:
1095 1049 261 32 32
XbaI T'CTAG_A
Cuts at: 0 2407 2469
Size: 2407 62
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 719 917 1275 2469
Size: 719 198 358 1194
Fragments arranged by size:
1194 719 358 198
XhoI C'TCGA_G
Cuts at: 0 2046 2469
Size: 2046 423
XmnI GAAnn'nnTTC
Cuts at: 0 1391 2469
Size: 1391 1078
Enzymes that do cut:
AarI AccI Acc65I AcuI AlfI AloI AlwNI ApoI
AseI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BfrBI BglI BglII Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI
BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtsI
DraI EaeI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I
HindIII KpnI MmeI MscI MslI MspA1I NcoI NheI
NsiI NspI PflMI PfoI PpiI PpuMI PshAI PspXI
PsrI PstI PvuII SacI SfcI SmlI StuI StyI
TaqII TstI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AleI
ApaI ApaLI AscI AsiSI AvrII BaeI BbeI BclI
BlpI BplI BsaAI BsaHI BsiEI BsiWI BspHI BsrBI
BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI
CspCI DraIII DrdI EagI EarI EciI EcoRI EcoRV
FseI FspI FspAI HaeII HincII HpaI KasI MfeI
MluI NaeI NarI NdeI NgoMIV NotI NruI PacI
PasI PciI PmeI PmlI PsiI PspOMI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TatI Tth111I XmaI ZraI