Restriction map for
pFN21ASDA0211
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# NO CUT ENZYMES ( 73 enzymes) #
##################################
AccI AclI AflII AgeI Aor51HI
AscI AseI AsnI Asp700I AspI
BbeI BciVI BfrI BlpI Bpu1102I
BsaBI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstZ17I CelII ClaI CpoI DraI
Eco47III EcoRI EcoRV FseI HpaI
KasI MamI MfeI MluI MunI
NarI NheI NotI NruI NspI
NspV PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SbfI ScaI
SfoI SfuI SgrAI SnaBI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 3234 GACGTC GACGT^C
AccIII 693 TCCGGA T^CCGGA
AhdI 2764 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2764 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3083 TGCGCA TGC^GCA
AvrII 808 CCTAGG C^CTAGG
BbrPI 1741 CACGTG CAC^GTG
BglII 294 AGATCT A^GATCT
BlnI 808 CCTAGG C^CTAGG
BsaI 3536 GAGACC GGTCTC(1/5)
BseAI 693 TCCGGA T^CCGGA
BsmBI 1778 GAGACG CGTCTC(1/5)
BsmI 2488 GCATTC GAATGC(1/-1)
BspEI 693 TCCGGA T^CCGGA
BsrBI 2331 CCGCTC CCGCTC(-3/-3)
BssSI 652 CACGAG CACGAG(-5/-1)
BstAPI 1850 GCACACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1220 GGTCACC G^GT[ACGT]ACC
BstPI 1220 GGTCACC G^GT[ACGT]ACC
EagI 191 CGGCCG C^GGCCG
Eam1105I 2764 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 191 CGGCCG C^GGCCG
Eco52I 191 CGGCCG C^GGCCG
EcoNI 1297 CCTGAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1220 GGTCACC G^GT[ACGT]ACC
EcoT22I 3202 ATGCAT ATGCA^T
FspI 3083 TGCGCA TGC^GCA
HaeII 1234 AGCGCC [GA]GCGC^[CT]
HincII 3749 GTCAAC GT[CT]^[GA]AC
HindII 3749 GTCAAC GT[CT]^[GA]AC
HindIII 719 AAGCTT A^AGCTT
KspI 2926 CCGCGG CCGC^GG
MroI 693 TCCGGA T^CCGGA
MseI 1139 TTAA T^TAA
NsiI 3202 ATGCAT ATGCA^T
PmaCI 1741 CACGTG CAC^GTG
PmlI 1741 CACGTG CAC^GTG
SacII 2926 CCGCGG CCGC^GG
SfiI 867 GGCCTTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 2845 ACTAGT A^CTAGT
Tru9I 1139 TTAA T^TAA
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 787 GGTACC G^GTACC
3035 GGTACC
AccII 2927 CGCG CG^CG
3378 CGCG
AcsI 325 AAATTT [GA]^AATT[CT]
1311 AAATTC
AcyI 2668 GGCGTC G[GA]^CG[CT]C
3234 GACGTC
AflIII 2727 ACACGT A^C[GA][CT]GT
2993 ACGTGT
Alw44I 1507 GTGCAC G^TGCAC
1848 GTGCAC
ApaLI 1507 GTGCAC G^TGCAC
1848 GTGCAC
ApoI 325 AAATTT [GA]^AATT[CT]
1311 AAATTC
Asp718I 787 GGTACC G^GTACC
3035 GGTACC
BcgI 3695 CGAGGAGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1922 GCAAGGGGCTCG
BclI 591 TGATCA T^GATCA
3741 TGATCA
BsaAI 1741 CACGTG [CT]AC^GT[GA]
2992 TACGTG
BsaHI 2668 GGCGTC G[GA]^CG[CT]C
3234 GACGTC
BsiEI 191 CGGCCG CG[GA][CT]^CG
3018 CGGTCG
BsrDI 1239 CATTGC GCAATG(2/0)
3326 GCAATG
BstUI 2927 CGCG CG^CG
3378 CGCG
DrdI 1557 GACAGCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2164 GACTACATGGTC
FbaI 591 TGATCA T^GATCA
3741 TGATCA
Hin1I 2668 GGCGTC G[GA]^CG[CT]C
3234 GACGTC
KpnI 787 GGTACC GGTAC^C
3035 GGTACC
MvnI 2927 CGCG CG^CG
3378 CGCG
NcoI 2498 CCATGG C^CATGG
3629 CCATGG
NdeI 135 CATATG CA^TATG
583 CATATG
PaeR7I 2819 CTCGAG C^TCGAG
3409 CTCGAG
PflMI 1947 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3292 CCAGTCAGTGG
SexAI 1625 ACCAGGT A^CC[AT]GGT
3437 ACCAGGT
SmaI 776 CCCGGG CCC^GGG
1400 CCCGGG
StuI 2893 AGGCCT AGG^CCT
3758 AGGCCT
Van91I 1947 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3292 CCAGTCAGTGG
XhoI 2819 CTCGAG C^TCGAG
3409 CTCGAG
XmaCI 776 CCCGGG C^CCGGG
1400 CCCGGG
XmaI 776 CCCGGG C^CCGGG
1400 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BalI BamHI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII