(Circular) (Six-base) MAPSORT of: pFN21ASDA0211.seq Check: 948 from: 1 to: 8530 pFN21ASDA0211 8530 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4675 5162 4675 Size: 487 8043 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5261 6340 278 Size: 53 83 186 936 3725 1079 2468 Fragments arranged by size: 3725 2468 1079 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5873 1937 Size: 3936 4594 Acc65I G'GTAC_C Cuts at: 2810 5058 5851 2810 Size: 2248 793 5489 Fragments arranged by size: 5489 2248 793 AclI AA'CG_TT Cuts at: 1563 6657 7030 1563 Size: 5094 373 3063 Fragments arranged by size: 5094 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3576 4794 4941 6593 7977 8245 1632 Size: 1944 1218 147 1652 1384 268 1917 Fragments arranged by size: 1944 1917 1652 1384 1218 268 147 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8333 AflIII A'CryG_T Cuts at: 1203 4750 5016 7429 8406 1203 Size: 3547 266 2413 977 1327 Fragments arranged by size: 3547 2413 1327 977 266 AgeI A'CCGG_T Cuts at: 7361 7361 Size: 8530 AhdI GACnn_n'nnGTC Cuts at: 4792 7259 4792 Size: 2467 6063 AleI CACnn'nnGTG Cuts at: 1166 2340 3930 4434 4506 4871 1166 Size: 1174 1590 504 72 365 4825 Fragments arranged by size: 4825 1590 1174 504 365 72 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3500 4910 1184 Size: 2316 1410 4804 Fragments arranged by size: 4804 2316 1410 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3989 4021 5070 5102 3989 Size: 32 1049 32 7417 Fragments arranged by size: 7417 1049 32 32 AlwNI CAG_nnn'CTG Cuts at: 2468 3215 3976 4130 4264 4287 6273 7845 2468 Size: 747 761 154 134 23 1986 1572 3153 Fragments arranged by size: 3153 1986 1572 761 747 154 134 23 ApaI G_GGCC'C Cuts at: 2964 3423 5735 2964 Size: 459 2312 5759 Fragments arranged by size: 5759 2312 459 ApaLI G'TGCA_C Cuts at: 3530 3871 6587 7743 3530 Size: 341 2716 1156 4317 Fragments arranged by size: 4317 2716 1156 341 ApoI r'AATT_y Cuts at: 1493 2348 3334 5839 6065 6217 8326 1493 Size: 855 986 2505 226 152 2109 1697 Fragments arranged by size: 2505 2109 1697 986 855 226 152 AseI AT'TA_AT Cuts at: 160 1032 7084 160 Size: 872 6052 1606 Fragments arranged by size: 6052 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8530 AvaI C'yCGr_G Cuts at: 1870 1943 2799 3423 4842 5425 5432 5855 1870 Size: 73 856 624 1419 583 7 423 4545 Fragments arranged by size: 4545 1419 856 624 583 423 73 7 AvrII C'CTAG_G Cuts at: 2831 2831 Size: 8530 BamHI G'GATC_C Cuts at: 2473 2713 3201 3759 5860 6227 2473 Size: 240 488 558 2101 367 4776 Fragments arranged by size: 4776 2101 558 488 367 240 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2810 3298 3634 4033 5058 Size: 325 217 627 1023 488 336 399 1025 Cuts at: 5058 5342 5647 5851 7306 618 Size: 284 305 204 1455 1842 Fragments arranged by size: 1842 1455 1025 1023 627 488 399 336 325 305 284 217 204 BanII G_rGCy'C Cuts at: 1391 2315 2964 3026 3347 3423 3954 5433 5636 Size: 924 649 62 321 76 531 1479 203 Cuts at: 5636 5735 5849 1391 Size: 99 114 4072 Fragments arranged by size: 4072 1479 924 649 531 321 203 114 99 76 62 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3582 3676 4621 4687 4800 928 Size: 971 1683 94 945 66 113 4658 Fragments arranged by size: 4658 1683 971 945 113 94 66 BbvCI CC'TCA_GC Cuts at: 4955 5454 5525 4955 Size: 499 71 7960 Fragments arranged by size: 7960 499 71 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3934 3968 5707 5741 6721 6755 1903 Size: 34 1997 34 1739 34 980 34 3678 Fragments arranged by size: 3678 1997 1739 980 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6422 7632 6422 Size: 1210 7320 BclI T'GATC_A Cuts at: 1548 2614 5764 1548 Size: 1066 3150 4314 Fragments arranged by size: 4314 3150 1066 BfrBI ATG'CAT Cuts at: 5227 5227 Size: 8530 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2105 2888 2897 Size: 107 122 71 631 605 433 783 9 Cuts at: 2897 4836 7141 136 Size: 1939 2305 1525 Fragments arranged by size: 2305 1939 1525 783 631 605 433 122 107 71 9 BglII A'GATC_T Cuts at: 2317 8525 2317 Size: 6208 2322 BlpI GC'TnA_GC Cuts at: 5947 5947 Size: 8530 Bme1580I G_kGCm'C Cuts at: 1790 2964 3423 3534 3639 3720 3875 4294 4706 Size: 1174 459 111 105 81 155 419 412 Cuts at: 4706 4715 5652 5735 6591 7747 1790 Size: 9 937 83 856 1156 2573 Fragments arranged by size: 2573 1174 1156 937 856 459 419 412 155 111 105 83 81 9 BmgBI CAC'GTC Cuts at: 1318 4753 1318 Size: 3435 5095 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2115 2280 2558 Size: 416 53 83 399 24 698 165 278 Cuts at: 2558 6261 7219 442 Size: 3703 958 1753 Fragments arranged by size: 3703 1753 958 698 416 399 278 165 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8530 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5650 5650 Size: 8530 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2136 2310 2423 2440 2591 3050 3812 4089 Size: 897 174 113 17 151 459 762 277 Cuts at: 4089 4787 5225 5322 7190 1239 Size: 698 438 97 1868 2579 Fragments arranged by size: 2579 1868 897 762 698 459 438 277 174 151 113 97 17 Bpu10I CC'TnA_GC Cuts at: 3827 4955 5454 5525 3827 Size: 1128 499 71 6832 Fragments arranged by size: 6832 1128 499 71 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4810 6655 7520 7818 4810 Size: 1845 865 298 5522 Fragments arranged by size: 5522 1845 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5553 7193 882 Size: 617 4054 1640 2219 Fragments arranged by size: 4054 2219 1640 617 BsaAI yAC'GTr Cuts at: 493 1204 3766 5017 5827 6321 493 Size: 711 2562 1251 810 494 2702 Fragments arranged by size: 2702 2562 1251 810 711 494 BsaBI GATnn'nnATC Cuts at: 8524 8524 Size: 8530 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4692 5258 6337 6719 Size: 53 83 186 936 3159 566 1079 382 Cuts at: 6719 7354 275 Size: 635 1451 Fragments arranged by size: 3159 1451 1079 936 635 566 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2590 2716 4143 6963 7361 7635 7782 8193 2590 Size: 126 1427 2820 398 274 147 411 2927 Fragments arranged by size: 2927 2820 1427 411 398 274 147 126 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2550 2580 4577 4607 1178 Size: 30 165 30 1147 30 1997 30 5101 Fragments arranged by size: 5101 1997 1147 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2141 4092 4241 5480 5582 5585 5588 5597 Size: 955 1951 149 1239 102 3 3 9 Cuts at: 5597 5642 5657 5734 1186 Size: 45 15 77 3982 Fragments arranged by size: 3982 1951 1239 955 149 102 77 45 15 9 3 3 BseYI C'CCAG_C Cuts at: 3275 3404 3969 4045 4566 4578 4596 4908 5180 Size: 129 565 76 521 12 18 312 272 Cuts at: 5180 5728 7733 8229 3275 Size: 548 2005 496 3576 Fragments arranged by size: 3576 2005 565 548 521 496 312 272 129 76 18 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3931 4188 4300 4379 4748 5149 1163 Size: 573 2195 257 112 79 369 401 4544 Fragments arranged by size: 4544 2195 573 401 369 257 112 79 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2217 5044 5980 6741 7769 1540 Size: 275 186 216 2827 936 761 1028 2301 Fragments arranged by size: 2827 2301 1028 936 761 275 216 186 BsiHKAI G_wGCw'C Cuts at: 3315 3479 3534 3875 3884 4616 4763 6591 6676 Size: 164 55 341 9 732 147 1828 85 Cuts at: 6676 7747 3315 Size: 1071 4098 Fragments arranged by size: 4098 1828 1071 732 341 164 147 85 55 9 BsmI GAATG_Cn' Cuts at: 4511 6041 6134 4511 Size: 1530 93 6907 Fragments arranged by size: 6907 1530 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3795 3795 Size: 8530 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2315 2964 3026 3315 3347 3423 3479 Size: 399 525 649 62 289 32 76 56 Cuts at: 3479 3534 3639 3720 3875 3884 3954 4294 4616 Size: 55 105 81 155 9 70 340 322 Cuts at: 4616 4706 4715 4763 5433 5636 5652 5735 5849 Size: 90 9 48 670 203 16 83 114 Cuts at: 5849 6591 6676 7747 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 670 649 525 399 340 322 289 203 155 114 105 90 85 83 81 76 70 62 56 55 48 32 16 9 9 BspEI T'CCGG_A Cuts at: 2716 8193 2716 Size: 5477 3053 BspHI T'CATG_A Cuts at: 6419 8149 6419 Size: 1730 6800 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2822 4253 4675 4982 5162 5885 844 Size: 1978 1431 422 307 180 723 3489 Fragments arranged by size: 3489 1978 1431 723 422 307 180 BsrBI CCG'CTC Cuts at: 1394 1823 4356 6417 1394 Size: 429 2533 2061 3507 Fragments arranged by size: 3507 2533 2061 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3261 5356 7025 7199 65 Size: 1183 2013 2095 1669 174 1396 Fragments arranged by size: 2095 2013 1669 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2212 3769 3843 5067 5687 7174 7361 Size: 110 402 1557 74 1224 620 1487 187 Cuts at: 7361 1700 Size: 2869 Fragments arranged by size: 2869 1557 1487 1224 620 402 187 110 74 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8530 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8530 BssSI C'ACGA_G Cuts at: 2675 6590 7602 2675 Size: 3915 1012 3603 Fragments arranged by size: 3915 3603 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3879 794 Size: 3085 5445 BstBI TT'CG_AA Cuts at: 2007 7334 2007 Size: 5327 3203 BstEII G'GTnAC_C Cuts at: 1184 3243 1184 Size: 2059 6471 BstXI CCAn_nnnn'nTGG Cuts at: 2342 2908 3839 4873 2342 Size: 566 931 1034 5999 Fragments arranged by size: 5999 1034 931 566 BstYI r'GATC_y Cuts at: 1972 2317 2329 2473 2713 2950 3201 3211 3759 Size: 345 12 144 240 237 251 10 548 Cuts at: 3759 5860 6227 6612 6629 8081 8167 8179 8525 Size: 2101 367 385 17 1452 86 12 346 Cuts at: 8525 1972 Size: 1977 Fragments arranged by size: 2101 1977 1452 548 385 367 346 345 251 240 237 144 86 17 12 12 10 Bsu36I CC'TnA_GG Cuts at: 2469 3321 5292 5742 5817 2469 Size: 852 1971 450 75 5182 Fragments arranged by size: 5182 1971 852 450 75 BtgI C'CryG_G Cuts at: 513 1065 2022 4029 4521 4765 4949 5038 5652 Size: 552 957 2007 492 244 184 89 614 Cuts at: 5652 513 Size: 3391 Fragments arranged by size: 3391 2007 957 614 552 492 244 184 89 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7791 Fragments arranged by size: 7791 683 56 BtsI GCAGTG_nn' Cuts at: 3088 3482 3968 4093 4486 4545 5078 5613 6046 Size: 394 486 125 393 59 533 535 433 Cuts at: 6046 6840 6860 3088 Size: 794 20 4758 Fragments arranged by size: 4758 794 535 533 486 433 394 393 125 59 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8530 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2074 2109 529 Size: 35 1510 35 6950 Fragments arranged by size: 6950 1510 35 35 DraI TTT'AAA Cuts at: 5834 5834 Size: 8530 DraIII CAC_nnn'GTG Cuts at: 2123 3652 3879 5464 5554 2123 Size: 1529 227 1585 90 5099 Fragments arranged by size: 5099 1585 1529 227 90 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3586 4193 7537 809 Size: 507 2270 607 3344 1802 Fragments arranged by size: 3344 2270 1802 607 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2214 2938 3537 Size: 54 1425 50 275 18 384 724 599 Cuts at: 3537 3771 3841 4903 5755 5977 6866 8495 8 Size: 234 70 1062 852 222 889 1629 43 Fragments arranged by size: 1629 1425 1062 889 852 724 599 384 275 234 222 70 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2214 5977 1537 Size: 275 402 3763 4090 Fragments arranged by size: 4090 3763 402 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3303 3815 3902 4634 5371 5543 5574 6460 Size: 1950 512 87 732 737 172 31 886 Cuts at: 6460 1353 Size: 3423 Fragments arranged by size: 3423 1950 886 737 732 512 172 87 31 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3862 7117 7491 7637 387 Size: 1064 138 2273 3255 374 146 1280 Fragments arranged by size: 3255 2273 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2136 2310 2423 2440 2591 3050 3576 Size: 393 504 174 113 17 151 459 526 Cuts at: 3576 3812 4089 4787 4794 4941 5225 5322 6593 Size: 236 277 698 7 147 284 97 1271 Cuts at: 6593 7190 7977 8245 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1271 787 698 597 526 504 459 393 284 277 268 236 174 151 147 113 97 17 7 EcoNI CCTnn'n_nnAGG Cuts at: 3324 3324 Size: 8530 EcoO109I rG'GnC_Cy Cuts at: 2960 3271 3420 3427 3694 4345 2960 Size: 311 149 7 267 651 7145 Fragments arranged by size: 7145 651 311 267 149 7 EcoRI G'AATT_C Cuts at: 5839 6217 5839 Size: 378 8152 FspI TGC'GCA Cuts at: 5108 7036 5108 Size: 1928 6602 HaeII r_GCGC'y Cuts at: 1678 3261 7677 1678 Size: 1583 4416 2531 Fragments arranged by size: 4416 2531 1583 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2550 2582 3383 3415 4778 Size: 32 135 32 978 32 801 32 1363 Cuts at: 4778 4808 4810 4840 5026 5058 5684 5716 7171 Size: 30 2 30 186 32 626 32 1455 Cuts at: 7171 7203 7245 7277 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1455 1363 978 801 626 186 135 42 32 32 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 669 1657 1938 5774 5874 6120 669 Size: 988 281 3836 100 246 3079 Fragments arranged by size: 3836 3079 988 281 246 100 HindIII A'AGCT_T Cuts at: 748 2742 748 Size: 1994 6536 HpaI GTT'AAC Cuts at: 6120 6120 Size: 8530 KpnI G_GTAC'C Cuts at: 2814 5062 5855 2814 Size: 2248 793 5489 Fragments arranged by size: 5489 2248 793 MfeI C'AATT_G Cuts at: 6129 6129 Size: 8530 MluI A'CGCG_T Cuts at: 8406 8406 Size: 8530 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3732 5024 7644 7828 1305 Size: 2427 1292 2620 184 2007 Fragments arranged by size: 2620 2427 2007 1292 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2940 3539 5757 10 Size: 54 1425 343 1108 599 2218 2783 Fragments arranged by size: 2783 2218 1425 1108 599 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2162 2340 3930 4434 4506 4871 6488 Size: 648 996 178 1590 504 72 365 1617 Cuts at: 6488 6847 7006 518 Size: 359 159 2042 Fragments arranged by size: 2042 1617 1590 996 648 504 365 359 178 159 72 MspA1I CmG'CkG Cuts at: 1606 3408 3973 4300 4814 4908 4951 5597 5705 Size: 1802 565 327 514 94 43 646 108 Cuts at: 5705 5946 6623 7771 8016 1606 Size: 241 677 1148 245 2120 Fragments arranged by size: 2120 1802 1148 677 646 565 514 327 245 241 108 94 43 NaeI GCC'GGC Cuts at: 2214 3771 3845 5069 2214 Size: 1557 74 1224 5675 Fragments arranged by size: 5675 1557 1224 74 NcoI C'CATG_G Cuts at: 513 1065 2022 4521 5652 513 Size: 552 957 2499 1131 3391 Fragments arranged by size: 3391 2499 1131 957 552 NdeI CA'TA_TG Cuts at: 387 2159 2607 387 Size: 1772 448 6310 Fragments arranged by size: 6310 1772 448 NgoMIV G'CCGG_C Cuts at: 2212 3769 3843 5067 2212 Size: 1557 74 1224 5675 Fragments arranged by size: 5675 1557 1224 74 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8530 NotI GC'GGCC_GC Cuts at: 5977 5977 Size: 8530 NsiI A_TGCA'T Cuts at: 5229 5229 Size: 8530 NspI r_CATG'y Cuts at: 1132 1229 5888 7433 1132 Size: 97 4659 1545 2229 Fragments arranged by size: 4659 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3349 1348 Size: 2001 6529 PciI A'CATG_T Cuts at: 7429 7429 Size: 8530 PflMI CCAn_nnn'nTGG Cuts at: 1108 3976 5321 1108 Size: 2868 1345 4317 Fragments arranged by size: 4317 2868 1345 PfoI T'CCnGG_A Cuts at: 2568 4476 2568 Size: 1908 6622 PmeI GTTT'AAAC Cuts at: 5834 5834 Size: 8530 PmlI CAC'GTG Cuts at: 3766 3766 Size: 8530 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6599 6631 8112 8144 6599 Size: 32 1481 32 6985 Fragments arranged by size: 6985 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3427 3694 4345 3427 Size: 267 651 7612 Fragments arranged by size: 7612 651 267 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8530 PsiI TTA'TAA Cuts at: 4608 6100 4608 Size: 1492 7038 PspOMI G'GGCC_C Cuts at: 2960 3419 5731 2960 Size: 459 2312 5759 Fragments arranged by size: 5759 2312 459 PspXI vC'TCGA_Gb Cuts at: 5432 5432 Size: 8530 PstI C_TGCA'G Cuts at: 830 3083 3413 3489 4552 5882 830 Size: 2253 330 76 1063 1330 3478 Fragments arranged by size: 3478 2253 1330 1063 330 76 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8530 PvuII CAG'CTG Cuts at: 3408 3973 5705 3408 Size: 565 1732 6233 Fragments arranged by size: 6233 1732 565 SacII CC_GC'GG Cuts at: 4952 4952 Size: 8530 SalI G'TCGA_C Cuts at: 1936 5872 1936 Size: 3936 4594 SanDI GG'GwC_CC Cuts at: 3427 3427 Size: 8530 SapI GCTCTTCn'nnn_ Cuts at: 3303 3815 3902 3303 Size: 512 87 7931 Fragments arranged by size: 7931 512 87 SbfI CC_TGCA'GG Cuts at: 5882 5882 Size: 8530 ScaI AGT'ACT Cuts at: 6778 8490 6778 Size: 1712 6818 SexAI A'CCwGG_T Cuts at: 3648 5460 3648 Size: 1812 6718 SfcI C'TryA_G Cuts at: 826 1045 3079 3409 3485 4417 4548 5878 7013 Size: 219 2034 330 76 932 131 1330 1135 Cuts at: 7013 7694 7885 826 Size: 681 191 1471 Fragments arranged by size: 2034 1471 1330 1135 932 681 330 219 191 131 76 SfiI GGCCn_nnn'nGGCC Cuts at: 2897 2897 Size: 8530 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8530 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8530 SmaI CCC'GGG Cuts at: 1872 2801 3425 5857 1872 Size: 929 624 2432 4545 Fragments arranged by size: 4545 2432 929 624 SmlI C'TyrA_G Cuts at: 820 1017 1943 4825 4842 5432 6634 7535 7797 Size: 197 926 2882 17 590 1202 901 262 Cuts at: 7797 820 Size: 1553 Fragments arranged by size: 2882 1553 1202 926 901 590 262 197 17 SnaBI TAC'GTA Cuts at: 493 5827 6321 493 Size: 5334 494 2702 Fragments arranged by size: 5334 2702 494 SpeI A'CTAG_T Cuts at: 152 4868 152 Size: 4716 3814 SphI G_CATG'C Cuts at: 1132 5888 1132 Size: 4756 3774 SspI AAT'ATT Cuts at: 5 52 6454 5 Size: 47 6402 2081 Fragments arranged by size: 6402 2081 47 StuI AGG'CCT Cuts at: 1517 4918 5783 1517 Size: 3401 865 4264 Fragments arranged by size: 4264 3401 865 StyI C'CwwG_G Cuts at: 513 1065 2022 2100 2655 2787 2831 2883 4452 Size: 552 957 78 555 132 44 52 1569 Cuts at: 4452 4521 5652 5969 513 Size: 69 1131 317 3074 Fragments arranged by size: 3074 1569 1131 957 555 552 317 132 78 69 52 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2134 2532 4580 4592 4778 6557 6574 Size: 1255 554 398 2048 12 186 1779 17 Cuts at: 6574 6727 6912 7357 325 Size: 153 185 445 1498 Fragments arranged by size: 2048 1779 1498 1255 554 445 398 186 185 153 17 12 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6776 8488 96 Size: 274 80 33 51 1443 4799 1712 138 Fragments arranged by size: 4799 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4577 4609 5795 5827 8490 8522 4577 Size: 32 1186 32 2663 32 4585 Fragments arranged by size: 4585 2663 1186 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8530 XbaI T'CTAG_A Cuts at: 5866 5866 Size: 8530 XhoI C'TCGA_G Cuts at: 1943 4842 5432 1943 Size: 2899 590 5041 Fragments arranged by size: 5041 2899 590 XmaI C'CCGG_G Cuts at: 1870 2799 3423 5855 1870 Size: 929 624 2432 4545 Fragments arranged by size: 4545 2432 929 624 XmnI GAAnn'nnTTC Cuts at: 6659 6659 Size: 8530 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5259 6338 276 Size: 53 83 186 936 3725 1079 2468 Fragments arranged by size: 3725 2468 1079 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbeI BsiWI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI PacI PsrI RsrII SacI SfoI SrfI SwaI XcmI