Restriction map for
pFN21ASDA0211
(Linear) (Six-base) MAPSORT of: KIAA0211.seq Check: 1036 from: 1 to: 3801
KIAA0211.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2652 3139 3801
Size: 2652 487 662
Fragments arranged by size:
2652 662 487
AatII G_ACGT'C
Cuts at: 0 3238 3801
Size: 3238 563
Acc65I G'GTAC_C
Cuts at: 0 787 3035 3801
Size: 787 2248 766
Fragments arranged by size:
2248 787 766
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1553 2771 2918 3801
Size: 1553 1218 147 883
Fragments arranged by size:
1553 1218 883 147
AflIII A'CryG_T
Cuts at: 0 2727 2993 3801
Size: 2727 266 808
Fragments arranged by size:
2727 808 266
AhdI GACnn_n'nnGTC
Cuts at: 0 2769 3801
Size: 2769 1032
AleI CACnn'nnGTG
Cuts at: 0 317 1907 2411 2483 2848 3801
Size: 317 1590 504 72 365 953
Fragments arranged by size:
1590 953 504 365 317 72
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1477 2887 3801
Size: 1477 1410 914
Fragments arranged by size:
1477 1410 914
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1966 1998 3047 3079 3801
Size: 1966 32 1049 32 722
Fragments arranged by size:
1966 1049 722 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 445 1192 1953 2107 2241 2264 3801
Size: 445 747 761 154 134 23 1537
Fragments arranged by size:
1537 761 747 445 154 134 23
ApaI G_GGCC'C
Cuts at: 0 941 1400 3712 3801
Size: 941 459 2312 89
Fragments arranged by size:
2312 941 459 89
ApaLI G'TGCA_C
Cuts at: 0 1507 1848 3801
Size: 1507 341 1953
Fragments arranged by size:
1953 1507 341
ApoI r'AATT_y
Cuts at: 0 325 1311 3801
Size: 325 986 2490
Fragments arranged by size:
2490 986 325
AvaI C'yCGr_G
Cuts at: 0 776 1400 2819 3402 3409 3801
Size: 776 624 1419 583 7 392
Fragments arranged by size:
1419 776 624 583 392 7
AvrII C'CTAG_G
Cuts at: 0 808 3801
Size: 808 2993
BamHI G'GATC_C
Cuts at: 0 450 690 1178 1736 3801
Size: 450 240 488 558 2065
Fragments arranged by size:
2065 558 488 450 240
BanI G'GyrC_C
Cuts at: 0 787 1275 1611 2010 3035 3319 3624 3801
Size: 787 488 336 399 1025 284 305 177
Fragments arranged by size:
1025 787 488 399 336 305 284 177
BanII G_rGCy'C
Cuts at: 0 292 941 1003 1324 1400 1931 3410 3613
Size: 292 649 62 321 76 531 1479 203
Cuts at: 3613 3712 3801
Size: 99 89
Fragments arranged by size:
1479 649 531 321 292 203 99 89
76 62
BbsI GAAGACnn'nnnn_
Cuts at: 0 1559 1653 2598 2664 2777 3801
Size: 1559 94 945 66 113 1024
Fragments arranged by size:
1559 1024 945 113 94 66
BbvCI CC'TCA_GC
Cuts at: 0 2932 3431 3502 3801
Size: 2932 499 71 299
Fragments arranged by size:
2932 499 299 71
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1911 1945 3684 3718 3801
Size: 1911 34 1739 34 83
Fragments arranged by size:
1911 1739 83 34 34
BclI T'GATC_A
Cuts at: 0 591 3741 3801
Size: 591 3150 60
Fragments arranged by size:
3150 591 60
BfrBI ATG'CAT
Cuts at: 0 3204 3801
Size: 3204 597
BglI GCCn_nnn'nGGC
Cuts at: 0 82 865 874 2813 3801
Size: 82 783 9 1939 988
Fragments arranged by size:
1939 988 783 82 9
BglII A'GATC_T
Cuts at: 0 294 3801
Size: 294 3507
Bme1580I G_kGCm'C
Cuts at: 0 941 1400 1511 1616 1697 1852 2271 2683
Size: 941 459 111 105 81 155 419 412
Cuts at: 2683 2692 3629 3712 3801
Size: 9 937 83 89
Fragments arranged by size:
941 937 459 419 412 155 111 105
89 83 81 9
BmgBI CAC'GTC
Cuts at: 0 2730 3801
Size: 2730 1071
BmrI ACTGGGnnnn_n'
Cuts at: 0 92 257 535 3801
Size: 92 165 278 3266
Fragments arranged by size:
3266 278 165 92
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3627 3801
Size: 3627 174
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 287 400 417 568 1027 1789 2066
Size: 113 174 113 17 151 459 762 277
Cuts at: 2066 2764 3202 3299 3801
Size: 698 438 97 502
Fragments arranged by size:
762 698 502 459 438 277 174 151
113 113 97 17
Bpu10I CC'TnA_GC
Cuts at: 0 1804 2932 3431 3502 3801
Size: 1804 1128 499 71 299
Fragments arranged by size:
1804 1128 499 299 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2787 3801
Size: 2787 1014
BsaI GGTCTCn'nnnn_
Cuts at: 0 3530 3801
Size: 3530 271
BsaAI yAC'GTr
Cuts at: 0 1743 2994 3801
Size: 1743 1251 807
Fragments arranged by size:
1743 1251 807
BsaHI Gr'CG_yC
Cuts at: 0 2669 3235 3801
Size: 2669 566 566
Fragments arranged by size:
2669 566 566
BsaWI w'CCGG_w
Cuts at: 0 567 693 2120 3801
Size: 567 126 1427 1681
Fragments arranged by size:
1681 1427 567 126
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 527 557 2554 2584 3801
Size: 527 30 1997 30 1217
Fragments arranged by size:
1997 1217 527 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 118 2069 2218 3457 3559 3562 3565 3574
Size: 118 1951 149 1239 102 3 3 9
Cuts at: 3574 3619 3634 3711 3801
Size: 45 15 77 90
Fragments arranged by size:
1951 1239 149 118 102 90 77 45
15 9 3 3
BseYI C'CCAG_C
Cuts at: 0 1252 1381 1946 2022 2543 2555 2573 2885
Size: 1252 129 565 76 521 12 18 312
Cuts at: 2885 3157 3705 3801
Size: 272 548 96
Fragments arranged by size:
1252 565 548 521 312 272 129 96
76 18 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1908 2165 2277 2356 2725 3126 3801
Size: 1908 257 112 79 369 401 675
Fragments arranged by size:
1908 675 401 369 257 112 79
BsiEI CG_ry'CG
Cuts at: 0 194 3021 3801
Size: 194 2827 780
Fragments arranged by size:
2827 780 194
BsiHKAI G_wGCw'C
Cuts at: 0 1292 1456 1511 1852 1861 2593 2740 3801
Size: 1292 164 55 341 9 732 147 1061
Fragments arranged by size:
1292 1061 732 341 164 147 55 9
BsmI GAATG_Cn'
Cuts at: 0 2488 3801
Size: 2488 1313
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1772 3801
Size: 1772 2029
Bsp1286I G_dGCh'C
Cuts at: 0 292 941 1003 1292 1324 1400 1456 1511
Size: 292 649 62 289 32 76 56 55
Cuts at: 1511 1616 1697 1852 1861 1931 2271 2593 2683
Size: 105 81 155 9 70 340 322 90
Cuts at: 2683 2692 2740 3410 3613 3629 3712 3801
Size: 9 48 670 203 16 83 89
Fragments arranged by size:
670 649 340 322 292 289 203 155
105 90 89 83 81 76 70 62
56 55 48 32 16 9 9
BspEI T'CCGG_A
Cuts at: 0 693 3801
Size: 693 3108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 799 2230 2652 2959 3139 3801
Size: 799 1431 422 307 180 662
Fragments arranged by size:
1431 799 662 422 307 180
BsrBI CCG'CTC
Cuts at: 0 2333 3801
Size: 2333 1468
BsrDI GCAATG_nn'
Cuts at: 0 1238 3333 3801
Size: 1238 2095 468
Fragments arranged by size:
2095 1238 468
BsrFI r'CCGG_y
Cuts at: 0 189 1746 1820 3044 3664 3801
Size: 189 1557 74 1224 620 137
Fragments arranged by size:
1557 1224 620 189 137 74
BssSI C'ACGA_G
Cuts at: 0 652 3801
Size: 652 3149
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1856 3801
Size: 1856 1945
BstEII G'GTnAC_C
Cuts at: 0 1220 3801
Size: 1220 2581
BstXI CCAn_nnnn'nTGG
Cuts at: 0 319 885 1816 2850 3801
Size: 319 566 931 1034 951
Fragments arranged by size:
1034 951 931 566 319
BstYI r'GATC_y
Cuts at: 0 294 306 450 690 927 1178 1188 1736
Size: 294 12 144 240 237 251 10 548
Cuts at: 1736 3801
Size: 2065
Fragments arranged by size:
2065 548 294 251 240 237 144 12
10
Bsu36I CC'TnA_GG
Cuts at: 0 446 1298 3269 3719 3794 3801
Size: 446 852 1971 450 75 7
Fragments arranged by size:
1971 852 450 446 75 7
BtgI C'CryG_G
Cuts at: 0 2006 2498 2742 2926 3015 3629 3801
Size: 2006 492 244 184 89 614 172
Fragments arranged by size:
2006 614 492 244 184 172 89
BtsI GCAGTG_nn'
Cuts at: 0 1065 1459 1945 2070 2463 2522 3055 3590
Size: 1065 394 486 125 393 59 533 535
Cuts at: 3590 3801
Size: 211
Fragments arranged by size:
1065 535 533 486 394 393 211 125
59
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 51 86 3801
Size: 51 35 3715
Fragments arranged by size:
3715 51 35
DraIII CAC_nnn'GTG
Cuts at: 0 100 1629 1856 3441 3531 3801
Size: 100 1529 227 1585 90 270
Fragments arranged by size:
1585 1529 270 227 100 90
DrdI GACnn_nn'nnGTC
Cuts at: 0 1563 2170 3801
Size: 1563 607 1631
Fragments arranged by size:
1631 1563 607
EaeI y'GGCC_r
Cuts at: 0 191 915 1514 1748 1818 2880 3732 3801
Size: 191 724 599 234 70 1062 852 69
Fragments arranged by size:
1062 852 724 599 234 191 70 69
EagI C'GGCC_G
Cuts at: 0 191 3801
Size: 191 3610
EarI CTCTTCn'nnn_
Cuts at: 0 1280 1792 1879 2611 3348 3520 3551 3801
Size: 1280 512 87 732 737 172 31 250
Fragments arranged by size:
1280 737 732 512 250 172 87 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1839 3801
Size: 1839 1962
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 287 400 417 568 1027 1553 1789
Size: 113 174 113 17 151 459 526 236
Cuts at: 1789 2066 2764 2771 2918 3202 3299 3801
Size: 277 698 7 147 284 97 502
Fragments arranged by size:
698 526 502 459 284 277 236 174
151 147 113 113 97 17 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1301 3801
Size: 1301 2500
EcoO109I rG'GnC_Cy
Cuts at: 0 937 1248 1397 1404 1671 2322 3801
Size: 937 311 149 7 267 651 1479
Fragments arranged by size:
1479 937 651 311 267 149 7
FspI TGC'GCA
Cuts at: 0 3085 3801
Size: 3085 716
HaeII r_GCGC'y
Cuts at: 0 1238 3801
Size: 1238 2563
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 527 559 1360 1392 2755 2785 2787 2817
Size: 527 32 801 32 1363 30 2 30
Cuts at: 2817 3003 3035 3661 3693 3801
Size: 186 32 626 32 108
Fragments arranged by size:
1363 801 626 527 186 108 32 32
32 32 30 30 2
HincII GTy'rAC
Cuts at: 0 3751 3801
Size: 3751 50
HindIII A'AGCT_T
Cuts at: 0 719 3801
Size: 719 3082
KpnI G_GTAC'C
Cuts at: 0 791 3039 3801
Size: 791 2248 762
Fragments arranged by size:
2248 791 762
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1709 3001 3801
Size: 1709 1292 800
Fragments arranged by size:
1709 1292 800
MscI TGG'CCA
Cuts at: 0 917 1516 3734 3801
Size: 917 599 2218 67
Fragments arranged by size:
2218 917 599 67
MslI CAynn'nnrTG
Cuts at: 0 139 317 1907 2411 2483 2848 3801
Size: 139 178 1590 504 72 365 953
Fragments arranged by size:
1590 953 504 365 178 139 72
MspA1I CmG'CkG
Cuts at: 0 1385 1950 2277 2791 2885 2928 3574 3682
Size: 1385 565 327 514 94 43 646 108
Cuts at: 3682 3801
Size: 119
Fragments arranged by size:
1385 646 565 514 327 119 108 94
43
NaeI GCC'GGC
Cuts at: 0 191 1748 1822 3046 3801
Size: 191 1557 74 1224 755
Fragments arranged by size:
1557 1224 755 191 74
NcoI C'CATG_G
Cuts at: 0 2498 3629 3801
Size: 2498 1131 172
Fragments arranged by size:
2498 1131 172
NdeI CA'TA_TG
Cuts at: 0 136 584 3801
Size: 136 448 3217
Fragments arranged by size:
3217 448 136
NgoMIV G'CCGG_C
Cuts at: 0 189 1746 1820 3044 3801
Size: 189 1557 74 1224 757
Fragments arranged by size:
1557 1224 757 189 74
NsiI A_TGCA'T
Cuts at: 0 3206 3801
Size: 3206 595
PasI CC'CwG_GG
Cuts at: 0 1326 3801
Size: 1326 2475
PflMI CCAn_nnn'nTGG
Cuts at: 0 1953 3298 3801
Size: 1953 1345 503
Fragments arranged by size:
1953 1345 503
PfoI T'CCnGG_A
Cuts at: 0 545 2453 3801
Size: 545 1908 1348
Fragments arranged by size:
1908 1348 545
PmlI CAC'GTG
Cuts at: 0 1743 3801
Size: 1743 2058
PpuMI rG'GwC_Cy
Cuts at: 0 1404 1671 2322 3801
Size: 1404 267 651 1479
Fragments arranged by size:
1479 1404 651 267
PsiI TTA'TAA
Cuts at: 0 2585 3801
Size: 2585 1216
PspOMI G'GGCC_C
Cuts at: 0 937 1396 3708 3801
Size: 937 459 2312 93
Fragments arranged by size:
2312 937 459 93
PspXI vC'TCGA_Gb
Cuts at: 0 3409 3801
Size: 3409 392
PstI C_TGCA'G
Cuts at: 0 1060 1390 1466 2529 3801
Size: 1060 330 76 1063 1272
Fragments arranged by size:
1272 1063 1060 330 76
PvuII CAG'CTG
Cuts at: 0 1385 1950 3682 3801
Size: 1385 565 1732 119
Fragments arranged by size:
1732 1385 565 119
SacII CC_GC'GG
Cuts at: 0 2929 3801
Size: 2929 872
SanDI GG'GwC_CC
Cuts at: 0 1404 3801
Size: 1404 2397
SapI GCTCTTCn'nnn_
Cuts at: 0 1280 1792 1879 3801
Size: 1280 512 87 1922
Fragments arranged by size:
1922 1280 512 87
SexAI A'CCwGG_T
Cuts at: 0 1625 3437 3801
Size: 1625 1812 364
Fragments arranged by size:
1812 1625 364
SfcI C'TryA_G
Cuts at: 0 1056 1386 1462 2394 2525 3801
Size: 1056 330 76 932 131 1276
Fragments arranged by size:
1276 1056 932 330 131 76
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 874 3801
Size: 874 2927
SmaI CCC'GGG
Cuts at: 0 778 1402 3801
Size: 778 624 2399
Fragments arranged by size:
2399 778 624
SmlI C'TyrA_G
Cuts at: 0 2802 2819 3409 3801
Size: 2802 17 590 392
Fragments arranged by size:
2802 590 392 17
SpeI A'CTAG_T
Cuts at: 0 2845 3801
Size: 2845 956
StuI AGG'CCT
Cuts at: 0 2895 3760 3801
Size: 2895 865 41
Fragments arranged by size:
2895 865 41
StyI C'CwwG_G
Cuts at: 0 77 632 764 808 860 2429 2498 3629
Size: 77 555 132 44 52 1569 69 1131
Cuts at: 3629 3801
Size: 172
Fragments arranged by size:
1569 1131 555 172 132 77 69 52
44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 111 509 2557 2569 2755 3801
Size: 111 398 2048 12 186 1046
Fragments arranged by size:
2048 1046 398 186 111 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2554 2586 3772 3801
Size: 2554 32 1186 29
Fragments arranged by size:
2554 1186 32 29
XhoI C'TCGA_G
Cuts at: 0 2819 3409 3801
Size: 2819 590 392
Fragments arranged by size:
2819 590 392
XmaI C'CCGG_G
Cuts at: 0 776 1400 3801
Size: 776 624 2401
Fragments arranged by size:
2401 776 624
ZraI GAC'GTC
Cuts at: 0 3236 3801
Size: 3236 565
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI
BanI BanII BbsI BbvCI BcgI BclI BfrBI BglI
BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtsI CspCI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII
HindIII KpnI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NsiI PasI PflMI PfoI PmlI PpuMI
PsiI PspOMI PspXI PstI PvuII SacII SanDI SapI
SexAI SfcI SfiI SmaI SmlI SpeI StuI StyI
TaqII TstI XhoI XmaI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AgeI AscI AseI AsiSI
BaeI BbeI BciVI BlpI BmtI BsaBI BsiWI BspHI
BsrGI BssHII BstBI BstZ17I BtgZI ClaI DraI EcoICRI
EcoRI EcoRV FalI FseI FspAI HpaI KasI MfeI
MluI NarI NheI NotI NruI NspI PacI PciI
PmeI PpiI PshAI PsrI PvuI RsrII SacI SalI
SbfI ScaI SfoI SgfI SgrAI SnaBI SphI SrfI
SspI SwaI TatI Tth111I XbaI XcmI XmnI