Restriction map for
pFN21ASDA0210
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflIII AgeI AocI Aor51HI
AscI Asp718I AspI AviII AvrII
BamHI BanI BbeI BbrPI BbsI
BcgI BciVI BcnI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspLU11I BsrBI BsrFI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV FauI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI KpnI
KspI MamI MluI MroI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PvuI RsrII
SacI SacII SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AclI 507 AACGTT AA^CGTT
AflII 1216 CTTAAG C^TTAAG
AhdI 64 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2115 GTGCAC G^TGCAC
AlwNI 1543 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 2289 GGGCCC GGGCC^C
ApaLI 2115 GTGCAC G^TGCAC
AspEI 64 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 378 CCCGAG C^[CT]CG[GA]G
BalI 393 TGGCCA TGG^CCA
BclI 410 TGATCA T^GATCA
BfrI 1216 CTTAAG C^TTAAG
BsaI 1368 GGTCTC GGTCTC(1/5)
BsoBI 378 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1200 TGTACA T^GTACA
BspHI 1316 TCATGA T^CATGA
BsrGI 1200 TGTACA T^GTACA
BssSI 1301 CACGAG CACGAG(-5/-1)
BstAPI 260 GCAGCATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Eam1105I 64 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 1177 ATGCAT ATGCA^T
FbaI 410 TGATCA T^GATCA
HincII 901 GTCAAC GT[CT]^[GA]AC
HindII 901 GTCAAC GT[CT]^[GA]AC
HindIII 665 AAGCTT A^AGCTT
MluNI 393 TGGCCA TGG^CCA
MscI 393 TGGCCA TGG^CCA
NsiI 1177 ATGCAT ATGCA^T
PshAI 301 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 507 AACGTT AA^CGTT
PvuII 238 CAGCTG CAG^CTG
RcaI 1316 TCATGA T^CATGA
SbfI 1032 CCTGCAGG CCTGCA^GG
Sse8387I 1032 CCTGCAGG CCTGCA^GG
SspBI 1200 TGTACA T^GTACA
SspI 1855 AATATT AAT^ATT
StuI 2093 AGGCCT AGG^CCT
TaqI 477 TCGA T^CGA
TthHB8I 477 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AfaI 1201 GTAC GT^AC
1444 GTAC
AseI 229 ATTAAT AT^TAAT
1384 ATTAAT
AsnI 229 ATTAAT AT^TAAT
1384 ATTAAT
Asp700I 122 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
450 GAACAAATTC
AspHI 1346 GTGCTC G[AT]GC[AT]^C
2115 GTGCAC
BanII 2184 GGGCTC G[GA]GC[CT]^C
2289 GGGCCC
BsgI 689 CTGCAC GTGCAG(16/14)
1430 GTGCAG
BsiHKAI 1346 GTGCTC G[AT]GC[AT]^C
2115 GTGCAC
BsmAI 1163 GTCTC GTCTC(1/5)
1369 GTCTC
BsmFI 148 GGGAC GGGAC(10/14)
2372 GTCCC
BspMI 251 GCAGGT ACCTGC(4/8)
1432 GCAGGT
BsrDI 2007 CATTGC GCAATG(2/0)
1414 GCAATG
EcoRI 1648 GAATTC G^AATTC
2130 GAATTC
EcoT14I 787 CCTTGG C^C[AT][AT]GG
2055 CCAAGG
HapII 1329 CCGG C^CGG
2195 CCGG
HpaII 1329 CCGG C^CGG
2195 CCGG
MaeII 508 ACGT A^CGT
1710 ACGT
MfeI 881 CAATTG C^AATTG
1576 CAATTG
MspA1I 238 CAGCTG C[AC]G^C[GT]G
2031 CAGCGG
MspI 1329 CCGG C^CGG
2195 CCGG
MunI 881 CAATTG C^AATTG
1576 CAATTG
PpuMI 1037 AGGACCT [GA]G^G[AT]CC[CT]
1492 AGGACCT
PshBI 229 ATTAAT AT^TAAT
1384 ATTAAT
PstI 34 CTGCAG CTGCA^G
1033 CTGCAG
RsaI 1201 GTAC GT^AC
1444 GTAC
SmlI 296 CTCAAG C^T[CT][GA]AG
1216 CTTAAG
SphI 614 GCATGC GCATG^C
1565 GCATGC
StyI 787 CCTTGG C^C[AT][AT]GG
2055 CCAAGG
Tsp45I 656 GTGAC ^GT[GC]AC
1261 GTGAC
XmnI 122 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
450 GAACAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BbvCI BbvI BfaI BmyI
BpmI BsaJI BseRI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI Sau3AI Sau96I ScrFI
SfaNI SfcI TfiI Tru9I TseI
Tsp509I TspRI XhoII