(Circular) (Six-base) MAPSORT of: pFN21ASDA0210.seq Check: 7486 from: 1 to: 7114 pFN21ASDA0210 7114 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3446 3446 Size: 7114 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4924 278 Size: 53 83 186 936 3388 2468 Fragments arranged by size: 3388 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4457 1937 Size: 2520 4594 Acc65I G'GTAC_C Cuts at: 4435 4435 Size: 7114 AclI AA'CG_TT Cuts at: 1563 2531 5241 5614 1563 Size: 968 2710 373 3063 Fragments arranged by size: 3063 2710 968 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2187 2969 3795 4107 5177 6561 6829 1632 Size: 555 782 826 312 1070 1384 268 1917 Fragments arranged by size: 1917 1384 1070 826 782 555 312 268 AflII C'TTAA_G Cuts at: 820 1017 3239 820 Size: 197 2222 4695 Fragments arranged by size: 4695 2222 197 AflIII A'CryG_T Cuts at: 1203 6013 6990 1203 Size: 4810 977 1327 Fragments arranged by size: 4810 1327 977 AgeI A'CCGG_T Cuts at: 5945 5945 Size: 7114 AhdI GACnn_n'nnGTC Cuts at: 2092 5843 2092 Size: 3751 3363 AleI CACnn'nnGTG Cuts at: 1166 4388 1166 Size: 3222 3892 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3611 1184 Size: 2427 4687 AlwNI CAG_nnn'CTG Cuts at: 3571 4857 6429 3571 Size: 1286 1572 4256 Fragments arranged by size: 4256 1572 1286 ApaI G_GGCC'C Cuts at: 4316 4316 Size: 7114 ApaLI G'TGCA_C Cuts at: 4138 5171 6327 4138 Size: 1033 1156 4925 Fragments arranged by size: 4925 1156 1033 ApoI r'AATT_y Cuts at: 1493 2477 2574 3304 3671 3795 3904 3924 4153 Size: 984 97 730 367 124 109 20 229 Cuts at: 4153 4423 4649 4801 6910 1493 Size: 270 226 152 2109 1697 Fragments arranged by size: 2109 1697 984 730 367 270 229 226 152 124 109 97 20 AseI AT'TA_AT Cuts at: 160 1032 2253 3408 5668 160 Size: 872 1221 1155 2260 1606 Fragments arranged by size: 2260 1606 1221 1155 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7114 AvaI C'yCGr_G Cuts at: 1870 1943 2401 4439 1870 Size: 73 458 2038 4545 Fragments arranged by size: 4545 2038 458 73 BamHI G'GATC_C Cuts at: 4444 4811 4444 Size: 367 6747 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4435 5890 618 Size: 325 217 627 2648 1455 1842 Fragments arranged by size: 2648 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4211 4316 4433 1391 Size: 2820 105 117 4072 Fragments arranged by size: 4072 2820 117 105 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6143 BbvCI CC'TCA_GC Cuts at: 3101 3520 4321 3101 Size: 419 801 5894 Fragments arranged by size: 5894 801 419 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5305 5339 1903 Size: 34 3368 34 3678 Fragments arranged by size: 3678 3368 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5006 6216 5006 Size: 1210 5904 BclI T'GATC_A Cuts at: 1548 2433 1548 Size: 885 6229 BfrBI ATG'CAT Cuts at: 3202 3202 Size: 7114 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5725 136 Size: 107 122 71 631 605 4053 1525 Fragments arranged by size: 4053 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7109 7109 Size: 7114 BlpI GC'TnA_GC Cuts at: 4531 4531 Size: 7114 Bme1580I G_kGCm'C Cuts at: 1790 4142 4316 5175 6331 1790 Size: 2352 174 859 1156 2573 Fragments arranged by size: 2573 2352 1156 859 174 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7114 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2715 4268 4845 Size: 416 53 83 399 24 1298 1553 577 Cuts at: 4845 5803 442 Size: 958 1753 Fragments arranged by size: 1753 1553 1298 958 577 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7114 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3353 3353 Size: 7114 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2136 3429 3492 3681 4036 4315 4330 5774 Size: 897 1293 63 189 355 279 15 1444 Cuts at: 5774 1239 Size: 2579 Fragments arranged by size: 2579 1444 1293 897 355 279 189 63 15 Bpu10I CC'TnA_GC Cuts at: 3101 3520 4321 3101 Size: 419 801 5894 Fragments arranged by size: 5894 801 419 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2304 5239 6104 6402 2304 Size: 2935 865 298 3016 Fragments arranged by size: 3016 2935 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3397 5777 882 Size: 617 1898 2380 2219 Fragments arranged by size: 2380 2219 1898 617 BsaAI yAC'GTr Cuts at: 493 1204 4411 4905 493 Size: 711 3207 494 2702 Fragments arranged by size: 3207 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7108 7108 Size: 7114 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4921 5303 5938 275 Size: 53 83 186 936 3388 382 635 1451 Fragments arranged by size: 3388 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5547 5945 6219 6366 6777 5547 Size: 398 274 147 411 5884 Fragments arranged by size: 5884 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3667 3697 4034 4064 4328 Size: 30 165 30 2264 30 337 30 264 Cuts at: 4328 4358 1178 Size: 30 3934 Fragments arranged by size: 3934 2264 337 264 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2133 3342 3481 3484 1186 Size: 947 1209 139 3 4816 Fragments arranged by size: 4816 1209 947 139 3 BseYI C'CCAG_C Cuts at: 3433 4227 4315 4340 6317 6813 3433 Size: 794 88 25 1977 496 3734 Fragments arranged by size: 3734 1977 794 496 88 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2697 3474 1163 Size: 573 961 777 4803 Fragments arranged by size: 4803 961 777 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4564 5325 6353 1540 Size: 275 186 2563 761 1028 2301 Fragments arranged by size: 2563 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3373 4142 5175 5260 6331 3373 Size: 769 1033 85 1071 4156 Fragments arranged by size: 4156 1071 1033 769 85 BsmI GAATG_Cn' Cuts at: 4625 4718 4625 Size: 93 7021 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3373 4142 4211 4316 4433 5175 5260 Size: 399 1583 769 69 105 117 742 85 Cuts at: 5260 6331 1391 Size: 1071 2174 Fragments arranged by size: 2174 1583 1071 769 742 399 117 105 85 69 BspEI T'CCGG_A Cuts at: 6777 6777 Size: 7114 BspHI T'CATG_A Cuts at: 3339 5003 6733 3339 Size: 1664 1730 3720 Fragments arranged by size: 3720 1730 1664 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2265 3446 4469 844 Size: 1421 1181 1023 3489 Fragments arranged by size: 3489 1421 1181 1023 BsrBI CCG'CTC Cuts at: 1394 1823 5001 1394 Size: 429 3178 3507 Fragments arranged by size: 3507 3178 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3444 4029 5609 5783 65 Size: 1183 2196 585 1580 174 1396 Fragments arranged by size: 2196 1580 1396 1183 585 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5758 5945 1700 Size: 110 3948 187 2869 Fragments arranged by size: 3948 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3223 96 Size: 3127 3987 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7114 BssSI C'ACGA_G Cuts at: 3324 5174 6186 3324 Size: 1850 1012 4252 Fragments arranged by size: 4252 1850 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2289 794 Size: 1495 5619 BstBI TT'CG_AA Cuts at: 2007 5918 2007 Size: 3911 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7114 BstYI r'GATC_y Cuts at: 1972 2931 3259 4300 4444 4811 5196 5213 6665 Size: 959 328 1041 144 367 385 17 1452 Cuts at: 6665 6751 6763 7109 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1041 959 385 367 346 328 144 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6562 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6375 Fragments arranged by size: 6375 683 56 BtsI GCAGTG_nn' Cuts at: 2784 3457 4204 4630 5424 5444 2784 Size: 673 747 426 794 20 4454 Fragments arranged by size: 4454 794 747 673 426 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7114 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7079 DraI TTT'AAA Cuts at: 4418 4418 Size: 7114 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6121 809 Size: 507 4805 1802 Fragments arranged by size: 4805 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2416 4057 4219 Size: 54 1425 50 275 18 586 1641 162 Cuts at: 4219 4561 5450 7079 8 Size: 342 889 1629 43 Fragments arranged by size: 1641 1629 1425 889 586 342 275 162 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4561 1537 Size: 275 2749 4090 Fragments arranged by size: 4090 2749 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3661 4005 4126 4239 5044 1353 Size: 2308 344 121 113 805 3423 Fragments arranged by size: 3423 2308 805 344 121 113 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5701 6075 6221 387 Size: 1064 138 4112 374 146 1280 Fragments arranged by size: 4112 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2136 2187 2969 3429 3492 3681 3795 Size: 393 504 51 782 460 63 189 114 Cuts at: 3795 4036 4107 4315 4330 5177 5774 6561 6829 Size: 241 71 208 15 847 597 787 268 Cuts at: 6829 1239 Size: 1524 Fragments arranged by size: 1524 847 787 782 597 504 460 393 268 241 208 189 114 71 63 51 15 EcoO109I rG'GnC_Cy Cuts at: 3061 3516 3529 4270 3061 Size: 455 13 741 5905 Fragments arranged by size: 5905 741 455 13 EcoRI G'AATT_C Cuts at: 3671 4153 4423 4801 3671 Size: 482 270 378 5984 Fragments arranged by size: 5984 482 378 270 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2172 2483 2172 Size: 311 6803 FspI TGC'GCA Cuts at: 5620 5620 Size: 7114 HaeII r_GCGC'y Cuts at: 1678 6261 1678 Size: 4583 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2078 2110 2425 2457 2923 Size: 32 135 32 506 32 315 32 466 Cuts at: 2923 2955 3212 3244 3850 3882 5755 5787 5829 Size: 32 257 32 606 32 1873 32 42 Cuts at: 5829 5861 1373 Size: 32 2626 Fragments arranged by size: 2626 1873 606 506 466 315 257 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2926 4458 4704 669 Size: 988 281 988 1532 246 3079 Fragments arranged by size: 3079 1532 988 988 281 246 HindIII A'AGCT_T Cuts at: 748 2688 748 Size: 1940 5174 HpaI GTT'AAC Cuts at: 4704 4704 Size: 7114 KpnI G_GTAC'C Cuts at: 4439 4439 Size: 7114 MfeI C'AATT_G Cuts at: 2904 3599 4713 2904 Size: 695 1114 5305 Fragments arranged by size: 5305 1114 695 MluI A'CGCG_T Cuts at: 6990 6990 Size: 7114 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6228 6412 1305 Size: 4923 184 2007 Fragments arranged by size: 4923 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2418 10 Size: 54 1425 343 586 4706 Fragments arranged by size: 4706 1425 586 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2703 3593 4388 5072 5431 5590 518 Size: 648 1537 890 795 684 359 159 2042 Fragments arranged by size: 2042 1537 890 795 684 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2263 4056 4530 5207 6355 6600 1606 Size: 657 1793 474 677 1148 245 2120 Fragments arranged by size: 2120 1793 1148 677 657 474 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6562 NdeI CA'TA_TG Cuts at: 387 387 Size: 7114 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7114 NotI GC'GGCC_GC Cuts at: 4561 4561 Size: 7114 NsiI A_TGCA'T Cuts at: 3204 3204 Size: 7114 NspI r_CATG'y Cuts at: 1132 1229 2641 2702 2977 3592 4472 6017 1132 Size: 97 1412 61 275 615 880 1545 2229 Fragments arranged by size: 2229 1545 1412 880 615 275 97 61 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7114 PciI A'CATG_T Cuts at: 6013 6013 Size: 7114 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7114 PfoI T'CCnGG_A Cuts at: 2934 2934 Size: 7114 PmeI GTTT'AAAC Cuts at: 4418 4418 Size: 7114 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5183 5215 6696 6728 5183 Size: 32 1481 32 5569 Fragments arranged by size: 5569 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3061 3516 3061 Size: 455 6659 PshAI GACnn'nnGTC Cuts at: 1613 2328 1613 Size: 715 6399 PsiI TTA'TAA Cuts at: 2628 4684 2628 Size: 2056 5058 PspOMI G'GGCC_C Cuts at: 4312 4312 Size: 7114 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2137 2169 2137 Size: 32 7082 PstI C_TGCA'G Cuts at: 830 2061 3060 4466 830 Size: 1231 999 1406 3478 Fragments arranged by size: 3478 1406 1231 999 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7114 PvuII CAG'CTG Cuts at: 2263 2263 Size: 7114 SalI G'TCGA_C Cuts at: 1936 4456 1936 Size: 2520 4594 SbfI CC_TGCA'GG Cuts at: 3060 4466 3060 Size: 1406 5708 ScaI AGT'ACT Cuts at: 5362 7074 5362 Size: 1712 5402 SfcI C'TryA_G Cuts at: 826 1045 2057 2185 2658 3056 4462 5597 6278 Size: 219 1012 128 473 398 1406 1135 681 Cuts at: 6278 6469 826 Size: 191 1471 Fragments arranged by size: 1471 1406 1135 1012 681 473 398 219 191 128 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7114 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7114 SmaI CCC'GGG Cuts at: 1872 4441 1872 Size: 2569 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2319 3239 5218 6119 6381 820 Size: 197 926 376 920 1979 901 262 1553 Fragments arranged by size: 1979 1553 926 920 901 376 262 197 SnaBI TAC'GTA Cuts at: 493 4411 4905 493 Size: 3918 494 2702 Fragments arranged by size: 3918 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7114 SphI G_CATG'C Cuts at: 1132 2641 3592 4472 1132 Size: 1509 951 880 3774 Fragments arranged by size: 3774 1509 951 880 SspI AAT'ATT Cuts at: 5 52 3880 5038 5 Size: 47 3828 1158 2081 Fragments arranged by size: 3828 2081 1158 47 StuI AGG'CCT Cuts at: 1517 4118 1517 Size: 2601 4513 StyI C'CwwG_G Cuts at: 513 1065 2810 4078 4553 513 Size: 552 1745 1268 475 3074 Fragments arranged by size: 3074 1745 1268 552 475 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3013 5141 5158 5311 5496 5941 325 Size: 1255 1433 2128 17 153 185 445 1498 Fragments arranged by size: 2128 1498 1433 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3223 5360 7072 Size: 274 80 33 51 1443 1246 2137 1712 Cuts at: 7072 96 Size: 138 Fragments arranged by size: 2137 1712 1443 1246 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3667 3699 3821 3853 4034 4066 7074 7106 3667 Size: 32 122 32 181 32 3008 32 3675 Fragments arranged by size: 3675 3008 181 122 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7114 XbaI T'CTAG_A Cuts at: 4450 4450 Size: 7114 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7114 XmaI C'CCGG_G Cuts at: 1870 4439 1870 Size: 2569 4545 XmnI GAAnn'nnTTC Cuts at: 2149 2477 5243 2149 Size: 328 2766 4020 Fragments arranged by size: 4020 2766 328 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4922 276 Size: 53 83 186 936 3388 2468 Fragments arranged by size: 3388 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI