Restriction map for
pFN21ASDA0210
(Linear) (Six-base) MAPSORT of: KIAA0210.seq Check: 2369 from: 1 to: 2385
KIAA0210.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1423 2385
Size: 1423 962
AclI AA'CG_TT
Cuts at: 0 508 2385
Size: 508 1877
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 946 1772 2084 2385
Size: 164 782 826 312 301
Fragments arranged by size:
826 782 312 301 164
AflII C'TTAA_G
Cuts at: 0 1216 2385
Size: 1216 1169
AhdI GACnn_n'nnGTC
Cuts at: 0 69 2385
Size: 69 2316
AleI CACnn'nnGTG
Cuts at: 0 2365 2385
Size: 2365 20
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1588 2385
Size: 1588 797
AlwNI CAG_nnn'CTG
Cuts at: 0 1548 2385
Size: 1548 837
ApaI G_GGCC'C
Cuts at: 0 2293 2385
Size: 2293 92
ApaLI G'TGCA_C
Cuts at: 0 2115 2385
Size: 2115 270
ApoI r'AATT_y
Cuts at: 0 454 551 1281 1648 1772 1881 1901 2130
Size: 454 97 730 367 124 109 20 229
Cuts at: 2130 2385
Size: 255
Fragments arranged by size:
730 454 367 255 229 124 109 97
20
AseI AT'TA_AT
Cuts at: 0 230 1385 2385
Size: 230 1155 1000
Fragments arranged by size:
1155 1000 230
AvaI C'yCGr_G
Cuts at: 0 378 2385
Size: 378 2007
BanII G_rGCy'C
Cuts at: 0 2188 2293 2385
Size: 2188 105 92
Fragments arranged by size:
2188 105 92
BbvCI CC'TCA_GC
Cuts at: 0 1078 1497 2298 2385
Size: 1078 419 801 87
Fragments arranged by size:
1078 801 419 87
BclI T'GATC_A
Cuts at: 0 410 2385
Size: 410 1975
BfrBI ATG'CAT
Cuts at: 0 1179 2385
Size: 1179 1206
Bme1580I G_kGCm'C
Cuts at: 0 2119 2293 2385
Size: 2119 174 92
Fragments arranged by size:
2119 174 92
BmrI ACTGGGnnnn_n'
Cuts at: 0 692 2245 2385
Size: 692 1553 140
Fragments arranged by size:
1553 692 140
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1330 2385
Size: 1330 1055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 1406 1469 1658 2013 2292 2307 2385
Size: 113 1293 63 189 355 279 15 78
Fragments arranged by size:
1293 355 279 189 113 78 63 15
Bpu10I CC'TnA_GC
Cuts at: 0 1078 1497 2298 2385
Size: 1078 419 801 87
Fragments arranged by size:
1078 801 419 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 2385
Size: 281 2104
BsaI GGTCTCn'nnnn_
Cuts at: 0 1374 2385
Size: 1374 1011
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1644 1674 2011 2041 2305 2335 2385
Size: 1644 30 337 30 264 30 50
Fragments arranged by size:
1644 337 264 50 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 110 1319 1458 1461 2385
Size: 110 1209 139 3 924
Fragments arranged by size:
1209 924 139 110 3
BseYI C'CCAG_C
Cuts at: 0 1410 2204 2292 2317 2385
Size: 1410 794 88 25 68
Fragments arranged by size:
1410 794 88 68 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 674 1451 2385
Size: 674 777 934
Fragments arranged by size:
934 777 674
BsiHKAI G_wGCw'C
Cuts at: 0 1350 2119 2385
Size: 1350 769 266
Fragments arranged by size:
1350 769 266
Bsp1286I G_dGCh'C
Cuts at: 0 1350 2119 2188 2293 2385
Size: 1350 769 69 105 92
Fragments arranged by size:
1350 769 105 92 69
BspHI T'CATG_A
Cuts at: 0 1316 2385
Size: 1316 1069
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 242 1423 2385
Size: 242 1181 962
Fragments arranged by size:
1181 962 242
BsrDI GCAATG_nn'
Cuts at: 0 1421 2006 2385
Size: 1421 585 379
Fragments arranged by size:
1421 585 379
BsrGI T'GTAC_A
Cuts at: 0 1200 2385
Size: 1200 1185
BssSI C'ACGA_G
Cuts at: 0 1301 2385
Size: 1301 1084
BstAPI GCAn_nnn'nTGC
Cuts at: 0 266 2385
Size: 266 2119
BstYI r'GATC_y
Cuts at: 0 908 1236 2277 2385
Size: 908 328 1041 108
Fragments arranged by size:
1041 908 328 108
BtsI GCAGTG_nn'
Cuts at: 0 761 1434 2181 2385
Size: 761 673 747 204
Fragments arranged by size:
761 747 673 204
EaeI y'GGCC_r
Cuts at: 0 393 2034 2196 2385
Size: 393 1641 162 189
Fragments arranged by size:
1641 393 189 162
EarI CTCTTCn'nnn_
Cuts at: 0 1638 1982 2103 2216 2385
Size: 1638 344 121 113 169
Fragments arranged by size:
1638 344 169 121 113
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 164 946 1406 1469 1658 1772 2013
Size: 113 51 782 460 63 189 114 241
Cuts at: 2013 2084 2292 2307 2385
Size: 71 208 15 78
Fragments arranged by size:
782 460 241 208 189 114 113 78
71 63 51 15
EcoO109I rG'GnC_Cy
Cuts at: 0 1038 1493 1506 2247 2385
Size: 1038 455 13 741 138
Fragments arranged by size:
1038 741 455 138 13
EcoRI G'AATT_C
Cuts at: 0 1648 2130 2385
Size: 1648 482 255
Fragments arranged by size:
1648 482 255
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 149 460 2385
Size: 149 311 1925
Fragments arranged by size:
1925 311 149
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 402 434 900 932 1189 1221
Size: 55 32 315 32 466 32 257 32
Cuts at: 1221 1827 1859 2385
Size: 606 32 526
Fragments arranged by size:
606 526 466 315 257 55 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 903 2385
Size: 903 1482
HindIII A'AGCT_T
Cuts at: 0 665 2385
Size: 665 1720
MfeI C'AATT_G
Cuts at: 0 881 1576 2385
Size: 881 695 809
Fragments arranged by size:
881 809 695
MscI TGG'CCA
Cuts at: 0 395 2385
Size: 395 1990
MslI CAynn'nnrTG
Cuts at: 0 680 1570 2365 2385
Size: 680 890 795 20
Fragments arranged by size:
890 795 680 20
MspA1I CmG'CkG
Cuts at: 0 240 2033 2385
Size: 240 1793 352
Fragments arranged by size:
1793 352 240
NsiI A_TGCA'T
Cuts at: 0 1181 2385
Size: 1181 1204
NspI r_CATG'y
Cuts at: 0 618 679 954 1569 2385
Size: 618 61 275 615 816
Fragments arranged by size:
816 618 615 275 61
PfoI T'CCnGG_A
Cuts at: 0 911 2385
Size: 911 1474
PpuMI rG'GwC_Cy
Cuts at: 0 1038 1493 2385
Size: 1038 455 892
Fragments arranged by size:
1038 892 455
PshAI GACnn'nnGTC
Cuts at: 0 305 2385
Size: 305 2080
PsiI TTA'TAA
Cuts at: 0 605 2385
Size: 605 1780
PspOMI G'GGCC_C
Cuts at: 0 2289 2385
Size: 2289 96
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 114 146 2385
Size: 114 32 2239
Fragments arranged by size:
2239 114 32
PstI C_TGCA'G
Cuts at: 0 38 1037 2385
Size: 38 999 1348
Fragments arranged by size:
1348 999 38
PvuII CAG'CTG
Cuts at: 0 240 2385
Size: 240 2145
SbfI CC_TGCA'GG
Cuts at: 0 1037 2385
Size: 1037 1348
SfcI C'TryA_G
Cuts at: 0 34 162 635 1033 2385
Size: 34 128 473 398 1352
Fragments arranged by size:
1352 473 398 128 34
SmlI C'TyrA_G
Cuts at: 0 296 1216 2385
Size: 296 920 1169
Fragments arranged by size:
1169 920 296
SphI G_CATG'C
Cuts at: 0 618 1569 2385
Size: 618 951 816
Fragments arranged by size:
951 816 618
SspI AAT'ATT
Cuts at: 0 1857 2385
Size: 1857 528
StuI AGG'CCT
Cuts at: 0 2095 2385
Size: 2095 290
StyI C'CwwG_G
Cuts at: 0 787 2055 2385
Size: 787 1268 330
Fragments arranged by size:
1268 787 330
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 990 2385
Size: 990 1395
TatI w'GTAC_w
Cuts at: 0 1200 2385
Size: 1200 1185
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1644 1676 1798 1830 2011 2043 2385
Size: 1644 32 122 32 181 32 342
Fragments arranged by size:
1644 342 181 122 32 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 126 454 2385
Size: 126 328 1931
Fragments arranged by size:
1931 328 126
Enzymes that do cut:
AarI AclI AcuI AflII AhdI AleI AlfI AlwNI
ApaI ApaLI ApoI AseI AvaI BanII BbvCI BclI
BfrBI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI
BsrDI BsrGI BssSI BstAPI BstYI BtsI EaeI EarI
Eco57MI EcoO109I EcoRI FalI Hin4I HincII HindIII MfeI
MscI MslI MspA1I NsiI NspI PfoI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuII SbfI SfcI SmlI
SphI SspI StuI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflIII AgeI AloI AscI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BcgI
BciVI BglI BglII BlpI BmgBI BmtI BsaAI BsaBI
BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BsrBI
BsrFI BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII HpaI
KasI KpnI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI PasI PciI PflMI
PmeI PmlI PpiI PspXI PvuI RsrII SacI SacII
SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SwaI Tth111I XbaI
XcmI XhoI XmaI ZraI