Restriction map for
pFN21ASDA0207
(Linear) (Six-base) MAPSORT of: KIAA0207.seq Check: 3164 from: 1 to: 1764
KIAA0207.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 986 1764
Size: 986 778
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 334 1629 1764
Size: 242 92 1295 135
Fragments arranged by size:
1295 242 135 92
AfeI AGC'GCT
Cuts at: 0 313 1764
Size: 313 1451
AflIII A'CryG_T
Cuts at: 0 1308 1764
Size: 1308 456
AlwNI CAG_nnn'CTG
Cuts at: 0 987 1764
Size: 987 777
ApaLI G'TGCA_C
Cuts at: 0 325 1764
Size: 325 1439
ApoI r'AATT_y
Cuts at: 0 706 759 828 857 1602 1669 1764
Size: 706 53 69 29 745 67 95
Fragments arranged by size:
745 706 95 69 67 53 29
AvaI C'yCGr_G
Cuts at: 0 251 294 1764
Size: 251 43 1470
Fragments arranged by size:
1470 251 43
AvrII C'CTAG_G
Cuts at: 0 633 1395 1764
Size: 633 762 369
Fragments arranged by size:
762 633 369
BanI G'GyrC_C
Cuts at: 0 210 344 788 1764
Size: 210 134 444 976
Fragments arranged by size:
976 444 210 134
BanII G_rGCy'C
Cuts at: 0 433 1067 1517 1764
Size: 433 634 450 247
Fragments arranged by size:
634 450 433 247
BbeI G_GCGC'C
Cuts at: 0 348 1764
Size: 348 1416
BbsI GAAGACnn'nnnn_
Cuts at: 0 365 662 1764
Size: 365 297 1102
Fragments arranged by size:
1102 365 297
BbvCI CC'TCA_GC
Cuts at: 0 1108 1211 1764
Size: 1108 103 553
Fragments arranged by size:
1108 553 103
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 34 68 142 176 1233 1267 1764
Size: 34 34 74 34 1057 34 497
Fragments arranged by size:
1057 497 74 34 34 34 34
BfrBI ATG'CAT
Cuts at: 0 163 1764
Size: 163 1601
BglII A'GATC_T
Cuts at: 0 928 1610 1764
Size: 928 682 154
Fragments arranged by size:
928 682 154
Bme1580I G_kGCm'C
Cuts at: 0 215 329 1764
Size: 215 114 1435
Fragments arranged by size:
1435 215 114
BmgBI CAC'GTC
Cuts at: 0 1145 1764
Size: 1145 619
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 825 1018 1369 1386 1461 1764
Size: 190 635 193 351 17 75 303
Fragments arranged by size:
635 351 303 193 190 75 17
Bpu10I CC'TnA_GC
Cuts at: 0 1108 1211 1764
Size: 1108 103 553
Fragments arranged by size:
1108 553 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1156 1720 1764
Size: 1156 564 44
Fragments arranged by size:
1156 564 44
BsaI GGTCTCn'nnnn_
Cuts at: 0 548 1764
Size: 548 1216
BsaBI GATnn'nnATC
Cuts at: 0 1390 1764
Size: 1390 374
BsaHI Gr'CG_yC
Cuts at: 0 345 1246 1764
Size: 345 901 518
Fragments arranged by size:
901 518 345
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 68 98 163 193 1000 1030 1764
Size: 68 30 65 30 807 30 734
Fragments arranged by size:
807 734 68 65 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 257 1159 1366 1525 1764
Size: 257 902 207 159 239
Fragments arranged by size:
902 257 239 207 159
BseYI C'CCAG_C
Cuts at: 0 317 815 885 1764
Size: 317 498 70 879
Fragments arranged by size:
879 498 317 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 1142 1764
Size: 328 814 622
Fragments arranged by size:
814 622 328
BsiHKAI G_wGCw'C
Cuts at: 0 329 443 623 1764
Size: 329 114 180 1141
Fragments arranged by size:
1141 329 180 114
BsmI GAATG_Cn'
Cuts at: 0 165 1187 1764
Size: 165 1022 577
Fragments arranged by size:
1022 577 165
Bsp1286I G_dGCh'C
Cuts at: 0 215 329 433 443 623 1067 1517 1764
Size: 215 114 104 10 180 444 450 247
Fragments arranged by size:
450 444 247 215 180 114 104 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 986 1764
Size: 986 778
BsrDI GCAATG_nn'
Cuts at: 0 1289 1764
Size: 1289 475
BsrFI r'CCGG_y
Cuts at: 0 10 387 1764
Size: 10 377 1377
Fragments arranged by size:
1377 377 10
BssSI C'ACGA_G
Cuts at: 0 1276 1516 1764
Size: 1276 240 248
Fragments arranged by size:
1276 248 240
BstAPI GCAn_nnn'nTGC
Cuts at: 0 306 1764
Size: 306 1458
BstYI r'GATC_y
Cuts at: 0 928 1472 1610 1764
Size: 928 544 138 154
Fragments arranged by size:
928 544 154 138
BtgI C'CryG_G
Cuts at: 0 689 1059 1764
Size: 689 370 705
Fragments arranged by size:
705 689 370
BtsI GCAGTG_nn'
Cuts at: 0 1263 1743 1764
Size: 1263 480 21
Fragments arranged by size:
1263 480 21
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 503 538 1764
Size: 503 35 1226
Fragments arranged by size:
1226 503 35
DraI TTT'AAA
Cuts at: 0 747 1764
Size: 747 1017
DraIII CAC_nnn'GTG
Cuts at: 0 591 1580 1764
Size: 591 989 184
Fragments arranged by size:
989 591 184
EaeI y'GGCC_r
Cuts at: 0 228 389 692 989 1764
Size: 228 161 303 297 775
Fragments arranged by size:
775 303 297 228 161
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1342 1764
Size: 1342 422
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 242 334 825 1018 1369 1386 1461
Size: 190 52 92 491 193 351 17 75
Cuts at: 1461 1629 1764
Size: 168 135
Fragments arranged by size:
491 351 193 190 168 135 92 75
52 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 352 1351 1764
Size: 352 999 413
Fragments arranged by size:
999 413 352
EcoO109I rG'GnC_Cy
Cuts at: 0 262 1764
Size: 262 1502
FspI TGC'GCA
Cuts at: 0 1255 1764
Size: 1255 509
HaeII r_GCGC'y
Cuts at: 0 315 348 1764
Size: 315 33 1416
Fragments arranged by size:
1416 315 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 68 100 1305 1337 1764
Size: 68 32 1205 32 427
Fragments arranged by size:
1205 427 68 32 32
HincII GTy'rAC
Cuts at: 0 61 1695 1764
Size: 61 1634 69
Fragments arranged by size:
1634 69 61
KasI G'GCGC_C
Cuts at: 0 344 1764
Size: 344 1420
MfeI C'AATT_G
Cuts at: 0 565 1764
Size: 565 1199
MluI A'CGCG_T
Cuts at: 0 1308 1764
Size: 1308 456
MscI TGG'CCA
Cuts at: 0 230 694 1764
Size: 230 464 1070
Fragments arranged by size:
1070 464 230
MslI CAynn'nnrTG
Cuts at: 0 1384 1753 1764
Size: 1384 369 11
Fragments arranged by size:
1384 369 11
MspA1I CmG'CkG
Cuts at: 0 436 605 984 1689 1707 1764
Size: 436 169 379 705 18 57
Fragments arranged by size:
705 436 379 169 57 18
NaeI GCC'GGC
Cuts at: 0 12 389 1764
Size: 12 377 1375
Fragments arranged by size:
1375 377 12
NarI GG'CG_CC
Cuts at: 0 345 1764
Size: 345 1419
NcoI C'CATG_G
Cuts at: 0 689 1764
Size: 689 1075
NgoMIV G'CCGG_C
Cuts at: 0 10 387 1764
Size: 10 377 1377
Fragments arranged by size:
1377 377 10
NsiI A_TGCA'T
Cuts at: 0 165 1764
Size: 165 1599
NspI r_CATG'y
Cuts at: 0 198 238 877 1764
Size: 198 40 639 887
Fragments arranged by size:
887 639 198 40
PasI CC'CwG_GG
Cuts at: 0 424 1764
Size: 424 1340
PflMI CCAn_nnn'nTGG
Cuts at: 0 570 666 1764
Size: 570 96 1098
Fragments arranged by size:
1098 570 96
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 366 398 1764
Size: 366 32 1366
Fragments arranged by size:
1366 366 32
PstI C_TGCA'G
Cuts at: 0 193 224 983 1764
Size: 193 31 759 781
Fragments arranged by size:
781 759 193 31
PvuII CAG'CTG
Cuts at: 0 605 984 1689 1707 1764
Size: 605 379 705 18 57
Fragments arranged by size:
705 605 379 57 18
SfcI C'TryA_G
Cuts at: 0 189 220 979 1052 1764
Size: 189 31 759 73 712
Fragments arranged by size:
759 712 189 73 31
SfoI GGC'GCC
Cuts at: 0 346 1764
Size: 346 1418
SmlI C'TyrA_G
Cuts at: 0 1171 1735 1764
Size: 1171 564 29
Fragments arranged by size:
1171 564 29
SpeI A'CTAG_T
Cuts at: 0 612 1764
Size: 612 1152
SphI G_CATG'C
Cuts at: 0 198 238 1764
Size: 198 40 1526
Fragments arranged by size:
1526 198 40
StuI AGG'CCT
Cuts at: 0 1225 1764
Size: 1225 539
StyI C'CwwG_G
Cuts at: 0 633 689 950 1395 1764
Size: 633 56 261 445 369
Fragments arranged by size:
633 445 369 261 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 116 260 1764
Size: 116 144 1504
Fragments arranged by size:
1504 144 116
TatI w'GTAC_w
Cuts at: 0 179 1040 1432 1569 1764
Size: 179 861 392 137 195
Fragments arranged by size:
861 392 195 179 137
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 799 1764
Size: 799 965
XmnI GAAnn'nnTTC
Cuts at: 0 763 1764
Size: 763 1001
Enzymes that do cut:
AarI AcuI AfeI AflIII AlwNI ApaLI ApoI AvaI
AvrII BanI BanII BbeI BbsI BbvCI BcgI BfrBI
BglII Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrDI BsrFI BssSI BstAPI BstYI BtgI BtsI
CspCI DraI DraIII EaeI EciI Eco57MI EcoNI EcoO109I
FspI HaeII Hin4I HincII KasI MfeI MluI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PflMI PpiI PstI PvuII SfcI SfoI SmlI
SpeI SphI StuI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApaI AscI AseI AsiSI BaeI BamHI
BciVI BclI BglI BlpI BmrI BmtI BplI BsaAI
BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrGI
BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
DrdI EagI EarI EcoICRI EcoRI EcoRV FalI FseI
FspAI HindIII HpaI KpnI MmeI NdeI NheI NotI
NruI PacI PciI PfoI PmeI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SrfI SspI SwaI TstI Tth111I
XbaI XhoI XmaI ZraI