Restriction map for
pFN21ASDA0202
(Circular) (Six-base) MAPSORT of: pFN21ASDA0202.seq Check: 7824 from: 1 to: 6016
pFN21ASDA0202 6016 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2803 2803
Size: 6016
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3826 278
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3359 1937
Size: 1422 4594
Acc65I G'GTAC_C
Cuts at: 3337 3337
Size: 6016
AclI AA'CG_TT
Cuts at: 1563 4143 4516 1563
Size: 2580 373 3063
Fragments arranged by size:
3063 2580 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2134 2450 2534 2965 3119 3161 4079 5463
Size: 502 316 84 431 154 42 918 1384
Cuts at: 5463 5731 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 918 502 431 316 268 154
84 42
AfeI AGC'GCT
Cuts at: 2045 2045
Size: 6016
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5819
AflIII A'CryG_T
Cuts at: 1203 4915 5892 1203
Size: 3712 977 1327
Fragments arranged by size:
3712 1327 977
AgeI A'CCGG_T
Cuts at: 4847 4847
Size: 6016
AhdI GACnn_n'nnGTC
Cuts at: 4745 4745
Size: 6016
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6016
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6016
AlwNI CAG_nnn'CTG
Cuts at: 3283 3759 5331 3283
Size: 476 1572 3968
Fragments arranged by size:
3968 1572 476
ApaLI G'TGCA_C
Cuts at: 4073 5229 4073
Size: 1156 4860
ApoI r'AATT_y
Cuts at: 1493 3325 3551 3703 5812 1493
Size: 1832 226 152 2109 1697
Fragments arranged by size:
2109 1832 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4570 160
Size: 872 3538 1606
Fragments arranged by size:
3538 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
AvaI C'yCGr_G
Cuts at: 1870 1943 3341 1870
Size: 73 1398 4545
Fragments arranged by size:
4545 1398 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2777 2810 2777
Size: 33 5983
BamHI G'GATC_C
Cuts at: 2469 3346 3713 2469
Size: 877 367 4772
Fragments arranged by size:
4772 877 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3337 4792 618
Size: 325 217 627 1550 1455 1842
Fragments arranged by size:
1842 1550 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2070 2610 2919 3120 3335 1391
Size: 679 540 309 201 215 4072
Fragments arranged by size:
4072 679 540 309 215 201
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2886 928
Size: 971 987 4058
Fragments arranged by size:
4058 987 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4207 4241 1903
Size: 34 2270 34 3678
Fragments arranged by size:
3678 2270 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3908 5118 3908
Size: 1210 4806
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6016
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2503 4627 136
Size: 107 122 71 631 605 831 2124 1525
Fragments arranged by size:
2124 1525 831 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2524 2661 6011 2524
Size: 137 3350 2529
Fragments arranged by size:
3350 2529 137
BlpI GC'TnA_GC
Cuts at: 3433 3433
Size: 6016
Bme1580I G_kGCm'C
Cuts at: 1790 2508 4077 5233 1790
Size: 718 1569 1156 2573
Fragments arranged by size:
2573 1569 1156 718
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6016
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3747 4705 442
Size: 416 53 83 399 24 2330 958 1753
Fragments arranged by size:
2330 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6016
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2065 2065
Size: 6016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 2276 3113 3215 4676 1239
Size: 821 216 837 102 1461 2579
Fragments arranged by size:
2579 1461 837 821 216 102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2297 4141 5006 5304 2297
Size: 1844 865 298 3009
Fragments arranged by size:
3009 1844 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2171 3197 4679 882
Size: 617 672 1026 1482 2219
Fragments arranged by size:
2219 1482 1026 672 617
BsaAI yAC'GTr
Cuts at: 493 1204 3313 3807 493
Size: 711 2109 494 2702
Fragments arranged by size:
2702 2109 711 494
BsaBI GATnn'nnATC
Cuts at: 2627 2861 6010 2627
Size: 234 3149 2633
Fragments arranged by size:
3149 2633 234
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3823 4205 4840 275
Size: 53 83 186 936 2290 382 635 1451
Fragments arranged by size:
2290 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4449 4847 5121 5268 5679 4449
Size: 398 274 147 411 4786
Fragments arranged by size:
4786 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2579 2609 1178
Size: 30 165 30 1176 30 4585
Fragments arranged by size:
4585 1176 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2438 2768 2954 3173 3212 3215 1186
Size: 1252 330 186 219 39 3 3987
Fragments arranged by size:
3987 1252 330 219 186 39 3
BseYI C'CCAG_C
Cuts at: 5219 5715 5219
Size: 496 5520
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2327 2831 1163
Size: 573 591 504 4348
Fragments arranged by size:
4348 591 573 504
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3466 4227 5255 1540
Size: 275 186 1465 761 1028 2301
Fragments arranged by size:
2301 1465 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2610 2788 2919 3120 3138 4077 4162 5233 2610
Size: 178 131 201 18 939 85 1071 3393
Fragments arranged by size:
3393 1071 939 201 178 131 85 18
BsmI GAATG_Cn'
Cuts at: 2059 2722 3527 3620 2059
Size: 663 805 93 4455
Fragments arranged by size:
4455 805 663 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2508 2610 2788 2919 3120 3138
Size: 399 280 438 102 178 131 201 18
Cuts at: 3138 3335 4077 4162 5233 1391
Size: 197 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 438 399 280 201 197
178 131 102 85 18
BspEI T'CCGG_A
Cuts at: 5679 5679
Size: 6016
BspHI T'CATG_A
Cuts at: 2712 3905 5635 2712
Size: 1193 1730 3093
Fragments arranged by size:
3093 1730 1193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2803 3371 844
Size: 1959 568 3489
Fragments arranged by size:
3489 1959 568
BsrBI CCG'CTC
Cuts at: 1394 1823 2442 3903 1394
Size: 429 619 1461 3507
Fragments arranged by size:
3507 1461 619 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4511 4685 65
Size: 1183 3263 174 1396
Fragments arranged by size:
3263 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2180 2904 2922 4660 4847 1700
Size: 110 370 724 18 1738 187 2869
Fragments arranged by size:
2869 1738 724 370 187 110 18
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6016
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6016
BssSI C'ACGA_G
Cuts at: 4076 5088 4076
Size: 1012 5004
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3259 794
Size: 2465 3551
BstBI TT'CG_AA
Cuts at: 2007 4820 2007
Size: 2813 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6016
BstXI CCAn_nnnn'nTGG
Cuts at: 2038 2038
Size: 6016
BstYI r'GATC_y
Cuts at: 1972 2433 2469 2524 2661 3346 3713 4098 4115
Size: 461 36 55 137 685 367 385 17
Cuts at: 4115 5567 5653 5665 6011 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 685 461 385 367 346 137
86 55 36 17 12
Bsu36I CC'TnA_GG
Cuts at: 3284 3284
Size: 6016
BtgI C'CryG_G
Cuts at: 513 1065 2022 2334 2676 2736 513
Size: 552 957 312 342 60 3793
Fragments arranged by size:
3793 957 552 342 312 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2560 2976 487
Size: 683 56 1334 416 3527
Fragments arranged by size:
3527 1334 683 416 56
BtsI GCAGTG_nn'
Cuts at: 2830 3532 4326 4346 2830
Size: 702 794 20 4500
Fragments arranged by size:
4500 794 702 20
ClaI AT'CG_AT
Cuts at: 1554 2394 1554
Size: 840 5176
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5981
DraI TTT'AAA
Cuts at: 3320 3320
Size: 6016
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2123 5023 809
Size: 507 807 2900 1802
Fragments arranged by size:
2900 1802 807 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2089 3463
Size: 54 1425 50 275 18 198 61 1374
Cuts at: 3463 4352 5981 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1374 889 275 198 61 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3463 1537
Size: 275 1651 4090
Fragments arranged by size:
4090 1651 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2219 2453 3042 3946 1353
Size: 866 234 589 904 3423
Fragments arranged by size:
3423 904 866 589 234
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2426 4603 4977 5123 387
Size: 1064 138 837 2177 374 146 1280
Fragments arranged by size:
2177 1280 1064 837 374 146 138
EcoICRI GAG'CTC
Cuts at: 2608 2917 3118 2608
Size: 309 201 5506
Fragments arranged by size:
5506 309 201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2134 2276 2450 2534 2965 3113
Size: 393 428 74 142 174 84 431 148
Cuts at: 3113 3119 3161 3215 4079 4676 5463 5731 1239
Size: 6 42 54 864 597 787 268 1524
Fragments arranged by size:
1524 864 787 597 431 428 393 268
174 148 142 84 74 54 42 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3287 3287
Size: 6016
EcoO109I rG'GnC_Cy
Cuts at: 3241 3241
Size: 6016
EcoRI G'AATT_C
Cuts at: 3325 3703 3325
Size: 378 5638
FspI TGC'GCA
Cuts at: 2400 4522 2400
Size: 2122 3894
HaeII r_GCGC'y
Cuts at: 1678 2047 2929 3187 5163 1678
Size: 369 882 258 1976 2531
Fragments arranged by size:
2531 1976 882 369 258
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2579 2611 4657 4689 4731
Size: 32 135 32 1007 32 2046 32 42
Cuts at: 4731 4763 1373
Size: 32 2626
Fragments arranged by size:
2626 2046 1007 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2386 3070 3360 3606 669
Size: 988 281 448 684 290 246 3079
Fragments arranged by size:
3079 988 684 448 290 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6016
HpaI GTT'AAC
Cuts at: 3606 3606
Size: 6016
KpnI G_GTAC'C
Cuts at: 3341 3341
Size: 6016
MfeI C'AATT_G
Cuts at: 3615 3615
Size: 6016
MluI A'CGCG_T
Cuts at: 5892 5892
Size: 6016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2916 5130 5314 1305
Size: 1611 2214 184 2007
Fragments arranged by size:
2214 2007 1611 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4194
Fragments arranged by size:
4194 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2201 3974 4333 4492 518
Size: 648 1035 1773 359 159 2042
Fragments arranged by size:
2042 1773 1035 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2122 3432 4109 5257 5502 1606
Size: 516 1310 677 1148 245 2120
Fragments arranged by size:
2120 1310 1148 677 516 245
NaeI GCC'GGC
Cuts at: 2906 2906
Size: 6016
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4507
Fragments arranged by size:
4507 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6016
NgoMIV G'CCGG_C
Cuts at: 2904 2904
Size: 6016
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6016
NotI GC'GGCC_GC
Cuts at: 3463 3463
Size: 6016
NspI r_CATG'y
Cuts at: 1132 1229 2262 3374 4919 1132
Size: 97 1033 1112 1545 2229
Fragments arranged by size:
2229 1545 1112 1033 97
PasI CC'CwG_GG
Cuts at: 1348 2083 2799 1348
Size: 735 716 4565
Fragments arranged by size:
4565 735 716
PciI A'CATG_T
Cuts at: 4915 4915
Size: 6016
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6016
PfoI T'CCnGG_A
Cuts at: 2292 2292
Size: 6016
PmeI GTTT'AAAC
Cuts at: 3320 3320
Size: 6016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4085 4117 5598 5630 4085
Size: 32 1481 32 4471
Fragments arranged by size:
4471 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6016
PsiI TTA'TAA
Cuts at: 3586 3586
Size: 6016
PstI C_TGCA'G
Cuts at: 830 2421 3036 3249 3368 830
Size: 1591 615 213 119 3478
Fragments arranged by size:
3478 1591 615 213 119
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6016
PvuII CAG'CTG
Cuts at: 2122 2122
Size: 6016
SacI G_AGCT'C
Cuts at: 2610 2919 3120 2610
Size: 309 201 5506
Fragments arranged by size:
5506 309 201
SalI G'TCGA_C
Cuts at: 1936 3358 1936
Size: 1422 4594
SapI GCTCTTCn'nnn_
Cuts at: 2453 2453
Size: 6016
SbfI CC_TGCA'GG
Cuts at: 3368 3368
Size: 6016
ScaI AGT'ACT
Cuts at: 4264 5976 4264
Size: 1712 4304
SfcI C'TryA_G
Cuts at: 826 1045 2417 3032 3245 3364 4499 5180 5371
Size: 219 1372 615 213 119 1135 681 191
Cuts at: 5371 826
Size: 1471
Fragments arranged by size:
1471 1372 1135 681 615 219 213 191
119
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6016
SmaI CCC'GGG
Cuts at: 1872 3343 1872
Size: 1471 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2312 4120 5021 5283 820
Size: 197 926 369 1808 901 262 1553
Fragments arranged by size:
1808 1553 926 901 369 262 197
SnaBI TAC'GTA
Cuts at: 493 3313 3807 493
Size: 2820 494 2702
Fragments arranged by size:
2820 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6016
SphI G_CATG'C
Cuts at: 1132 2262 3374 1132
Size: 1130 1112 3774
Fragments arranged by size:
3774 1130 1112
SspI AAT'ATT
Cuts at: 5 52 3940 5
Size: 47 3888 2081
Fragments arranged by size:
3888 2081 47
StuI AGG'CCT
Cuts at: 1517 3257 1517
Size: 1740 4276
StyI C'CwwG_G
Cuts at: 513 1065 2022 2580 3455 513
Size: 552 957 558 875 3074
Fragments arranged by size:
3074 957 875 558 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2125 4043 4060 4213 4398 4843 325
Size: 1255 545 1918 17 153 185 445 1498
Fragments arranged by size:
1918 1498 1255 545 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4262 5974 96
Size: 274 80 33 51 1443 2285 1712 138
Fragments arranged by size:
2285 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5976 6008 5976
Size: 32 5984
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6016
XbaI T'CTAG_A
Cuts at: 2521 3352 2521
Size: 831 5185
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6016
XmaI C'CCGG_G
Cuts at: 1870 3341 1870
Size: 1471 4545
XmnI GAAnn'nnTTC
Cuts at: 3017 4145 3017
Size: 1128 4888
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3824 276
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI