(Circular) (Six-base) MAPSORT of: pFN21ASDA0201.seq Check: 7347 from: 1 to: 7303 pFN21ASDA0201 7303 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5113 278 Size: 53 83 186 936 3577 2468 Fragments arranged by size: 3577 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4646 1937 Size: 2709 4594 Acc65I G'GTAC_C Cuts at: 4624 4624 Size: 7303 AclI AA'CG_TT Cuts at: 1563 5430 5803 1563 Size: 3867 373 3063 Fragments arranged by size: 3867 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2417 3282 3573 4059 4170 4204 5366 6750 Size: 785 865 291 486 111 34 1162 1384 Cuts at: 6750 7018 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1162 865 785 486 291 268 111 34 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7106 AflIII A'CryG_T Cuts at: 1203 3858 4406 6202 7179 1203 Size: 2655 548 1796 977 1327 Fragments arranged by size: 2655 1796 1327 977 548 AgeI A'CCGG_T Cuts at: 2118 6134 2118 Size: 4016 3287 AhdI GACnn_n'nnGTC Cuts at: 6032 6032 Size: 7303 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7303 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4215 1184 Size: 3031 4272 AlwNI CAG_nnn'CTG Cuts at: 2474 3602 3731 4037 4189 5046 6618 2474 Size: 1128 129 306 152 857 1572 3159 Fragments arranged by size: 3159 1572 1128 857 306 152 129 ApaLI G'TGCA_C Cuts at: 2122 5360 6516 2122 Size: 3238 1156 2909 Fragments arranged by size: 3238 2909 1156 ApoI r'AATT_y Cuts at: 1493 2568 2762 3060 3089 3194 3986 4012 4573 Size: 1075 194 298 29 105 792 26 561 Cuts at: 4573 4612 4838 4990 7099 1493 Size: 39 226 152 2109 1697 Fragments arranged by size: 2109 1697 1075 792 561 298 226 194 152 105 39 29 26 AseI AT'TA_AT Cuts at: 160 1032 4106 5857 160 Size: 872 3074 1751 1606 Fragments arranged by size: 3074 1751 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7303 AvaI C'yCGr_G Cuts at: 1870 1943 2076 4628 1870 Size: 73 133 2552 4545 Fragments arranged by size: 4545 2552 133 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2804 2837 2804 Size: 33 7270 BamHI G'GATC_C Cuts at: 4351 4633 5000 4351 Size: 282 367 6654 Fragments arranged by size: 6654 367 282 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4624 6079 618 Size: 325 217 627 2837 1455 1842 Fragments arranged by size: 2837 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2051 2858 4622 1391 Size: 660 807 1764 4072 Fragments arranged by size: 4072 1764 807 660 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3540 4047 4515 928 Size: 971 1641 507 468 3716 Fragments arranged by size: 3716 1641 971 507 468 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5494 5528 1903 Size: 34 3557 34 3678 Fragments arranged by size: 3678 3557 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5195 6405 5195 Size: 1210 6093 BclI T'GATC_A Cuts at: 1548 4345 1548 Size: 2797 4506 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5914 136 Size: 107 122 71 631 605 4242 1525 Fragments arranged by size: 4242 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7298 7298 Size: 7303 BlpI GC'TnA_GC Cuts at: 4720 4720 Size: 7303 Bme1580I G_kGCm'C Cuts at: 1790 2126 5364 6520 1790 Size: 336 3238 1156 2573 Fragments arranged by size: 3238 2573 1156 336 BmgBI CAC'GTC Cuts at: 1318 2044 1318 Size: 726 6577 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2699 5034 5992 Size: 416 53 83 399 24 1282 2335 958 Cuts at: 5992 442 Size: 1753 Fragments arranged by size: 2335 1753 1282 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7303 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3750 4107 5963 1239 Size: 2511 357 1856 2579 Fragments arranged by size: 2579 2511 1856 357 Bpu10I CC'TnA_GC Cuts at: 4326 4368 4326 Size: 42 7261 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2302 2405 5428 6293 6591 2302 Size: 103 3023 865 298 3014 Fragments arranged by size: 3023 3014 865 298 103 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2087 3574 5966 882 Size: 617 588 1487 2392 2219 Fragments arranged by size: 2392 2219 1487 617 588 BsaAI yAC'GTr Cuts at: 493 1204 4364 4600 5094 493 Size: 711 3160 236 494 2702 Fragments arranged by size: 3160 2702 711 494 236 BsaBI GATnn'nnATC Cuts at: 4350 7297 4350 Size: 2947 4356 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5110 5492 6127 275 Size: 53 83 186 936 3577 382 635 1451 Fragments arranged by size: 3577 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2118 2915 5736 6134 6408 6555 6966 2118 Size: 797 2821 398 274 147 411 2455 Fragments arranged by size: 2821 2455 797 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2793 2823 1178 Size: 30 165 30 1390 30 5658 Fragments arranged by size: 5658 1390 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3548 4016 1186 Size: 2362 468 4473 Fragments arranged by size: 4473 2362 468 BseYI C'CCAG_C Cuts at: 6506 7002 6506 Size: 496 6807 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2484 2778 3051 3411 1163 Size: 573 748 294 273 360 5055 Fragments arranged by size: 5055 748 573 360 294 273 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2119 4753 5514 6542 1540 Size: 275 186 118 2634 761 1028 2301 Fragments arranged by size: 2634 2301 1028 761 275 186 118 BsiHKAI G_wGCw'C Cuts at: 2126 2809 2858 4369 5364 5449 6520 2126 Size: 683 49 1511 995 85 1071 2909 Fragments arranged by size: 2909 1511 1071 995 683 85 49 BsmI GAATG_Cn' Cuts at: 2243 2894 4327 4814 4907 2243 Size: 651 1433 487 93 4639 Fragments arranged by size: 4639 1433 651 487 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 2126 2809 2858 4369 4622 5364 Size: 399 261 75 683 49 1511 253 742 Cuts at: 5364 5449 6520 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1511 1071 742 683 399 261 253 85 75 49 BspEI T'CCGG_A Cuts at: 2915 6966 2915 Size: 4051 3252 BspHI T'CATG_A Cuts at: 3271 4317 5192 6922 3271 Size: 1046 875 1730 3652 Fragments arranged by size: 3652 1730 1046 875 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4658 844 Size: 3814 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5190 1394 Size: 429 3367 3507 Fragments arranged by size: 3507 3367 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5798 5972 65 Size: 1183 4550 174 1396 Fragments arranged by size: 4550 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2118 5947 6134 1700 Size: 110 308 3829 187 2869 Fragments arranged by size: 3829 2869 308 187 110 BsrGI T'GTAC_A Cuts at: 96 2334 96 Size: 2238 5065 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7303 BssSI C'ACGA_G Cuts at: 5363 6375 5363 Size: 1012 6291 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7303 BstBI TT'CG_AA Cuts at: 2007 6107 2007 Size: 4100 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7303 BstXI CCAn_nnnn'nTGG Cuts at: 2029 2029 Size: 7303 BstYI r'GATC_y Cuts at: 1972 2581 4351 4633 5000 5385 5402 6854 6940 Size: 609 1770 282 367 385 17 1452 86 Cuts at: 6940 6952 7298 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1770 1452 609 385 367 346 282 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3481 513 Size: 552 2416 4335 Fragments arranged by size: 4335 2416 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2050 2083 487 Size: 683 56 824 33 5707 Fragments arranged by size: 5707 824 683 56 33 BtsI GCAGTG_nn' Cuts at: 4819 5613 5633 4819 Size: 794 20 6489 Fragments arranged by size: 6489 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7303 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3952 3987 529 Size: 35 3388 35 3845 Fragments arranged by size: 3845 3388 35 35 DraI TTT'AAA Cuts at: 2767 3184 4607 2767 Size: 417 1423 5463 Fragments arranged by size: 5463 1423 417 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6310 809 Size: 507 4994 1802 Fragments arranged by size: 4994 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2236 4157 4750 Size: 54 1425 50 275 18 406 1921 593 Cuts at: 4750 5639 7268 8 Size: 889 1629 43 Fragments arranged by size: 1921 1629 1425 889 593 406 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4750 1537 Size: 275 2938 4090 Fragments arranged by size: 4090 2938 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5233 1353 Size: 3880 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5890 6264 6410 387 Size: 1064 138 4301 374 146 1280 Fragments arranged by size: 4301 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2856 2856 Size: 7303 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2417 3282 3573 3750 4059 4107 4170 Size: 393 785 865 291 177 309 48 63 Cuts at: 4170 4204 5366 5963 6750 7018 1239 Size: 34 1162 597 787 268 1524 Fragments arranged by size: 1524 1162 865 787 785 597 393 309 291 268 177 63 48 34 EcoNI CCTnn'n_nnAGG Cuts at: 2712 2712 Size: 7303 EcoO109I rG'GnC_Cy Cuts at: 3335 3335 Size: 7303 EcoRI G'AATT_C Cuts at: 3060 4612 4990 3060 Size: 1552 378 5373 Fragments arranged by size: 5373 1552 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2296 2999 3354 3869 2296 Size: 703 355 515 5730 Fragments arranged by size: 5730 703 515 355 FspI TGC'GCA Cuts at: 5809 5809 Size: 7303 HaeII r_GCGC'y Cuts at: 1678 3054 6450 1678 Size: 1376 3396 2531 Fragments arranged by size: 3396 2531 1376 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2033 2065 2468 2500 2777 Size: 32 135 32 461 32 403 32 277 Cuts at: 2777 2809 3444 3476 4330 4362 5944 5976 6018 Size: 32 635 32 854 32 1582 32 42 Cuts at: 6018 6050 1373 Size: 32 2626 Fragments arranged by size: 2626 1582 854 635 461 403 277 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2388 3475 3757 4090 4647 4893 Size: 988 281 450 1087 282 333 557 246 Cuts at: 4893 669 Size: 3079 Fragments arranged by size: 3079 1087 988 557 450 333 282 281 246 HindIII A'AGCT_T Cuts at: 748 3351 748 Size: 2603 4700 HpaI GTT'AAC Cuts at: 4893 4893 Size: 7303 KpnI G_GTAC'C Cuts at: 4628 4628 Size: 7303 MfeI C'AATT_G Cuts at: 4902 4902 Size: 7303 MluI A'CGCG_T Cuts at: 7179 7179 Size: 7303 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2019 2299 2763 2976 3025 4077 6417 6601 Size: 714 280 464 213 49 1052 2340 184 Cuts at: 6601 1305 Size: 2007 Fragments arranged by size: 2340 2007 1052 714 464 280 213 184 49 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5481 Fragments arranged by size: 5481 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 2754 3157 4251 4545 5261 5620 Size: 648 861 727 403 1094 294 716 359 Cuts at: 5620 5779 518 Size: 159 2042 Fragments arranged by size: 2042 1094 861 727 716 648 403 359 294 159 MspA1I CmG'CkG Cuts at: 1606 2549 3068 4214 4719 5396 6544 6789 1606 Size: 943 519 1146 505 677 1148 245 2120 Fragments arranged by size: 2120 1148 1146 943 677 519 505 245 NcoI C'CATG_G Cuts at: 513 1065 3481 513 Size: 552 2416 4335 Fragments arranged by size: 4335 2416 552 NdeI CA'TA_TG Cuts at: 387 3979 4084 387 Size: 3592 105 3606 Fragments arranged by size: 3606 3592 105 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7303 NotI GC'GGCC_GC Cuts at: 4750 4750 Size: 7303 NspI r_CATG'y Cuts at: 1132 1229 3862 4410 4661 6206 1132 Size: 97 2633 548 251 1545 2229 Fragments arranged by size: 2633 2229 1545 548 251 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7303 PciI A'CATG_T Cuts at: 3858 4406 6202 3858 Size: 548 1796 4959 Fragments arranged by size: 4959 1796 548 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7303 PmeI GTTT'AAAC Cuts at: 4607 4607 Size: 7303 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5372 5404 6885 6917 5372 Size: 32 1481 32 5758 Fragments arranged by size: 5758 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7303 PsiI TTA'TAA Cuts at: 2574 4873 2574 Size: 2299 5004 PstI C_TGCA'G Cuts at: 830 4194 4655 830 Size: 3364 461 3478 Fragments arranged by size: 3478 3364 461 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7303 PvuII CAG'CTG Cuts at: 2549 3068 4214 2549 Size: 519 1146 5638 Fragments arranged by size: 5638 1146 519 SacI G_AGCT'C Cuts at: 2858 2858 Size: 7303 SalI G'TCGA_C Cuts at: 1936 4645 1936 Size: 2709 4594 SbfI CC_TGCA'GG Cuts at: 4655 4655 Size: 7303 ScaI AGT'ACT Cuts at: 5551 7263 5551 Size: 1712 5591 SfcI C'TryA_G Cuts at: 826 1045 4190 4651 5786 6467 6658 826 Size: 219 3145 461 1135 681 191 1471 Fragments arranged by size: 3145 1471 1135 681 461 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7303 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7303 SmaI CCC'GGG Cuts at: 1872 2078 4630 1872 Size: 206 2552 4545 Fragments arranged by size: 4545 2552 206 SmlI C'TyrA_G Cuts at: 820 1017 1943 2281 2420 5407 6308 6570 820 Size: 197 926 338 139 2987 901 262 1553 Fragments arranged by size: 2987 1553 926 901 338 262 197 139 SnaBI TAC'GTA Cuts at: 493 4600 5094 493 Size: 4107 494 2702 Fragments arranged by size: 4107 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7303 SphI G_CATG'C Cuts at: 1132 4661 1132 Size: 3529 3774 SrfI GCCC'GGGC Cuts at: 2078 2078 Size: 7303 SspI AAT'ATT Cuts at: 5 52 4483 5227 5 Size: 47 4431 744 2081 Fragments arranged by size: 4431 2081 744 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7303 StyI C'CwwG_G Cuts at: 513 1065 3370 3481 4742 513 Size: 552 2305 111 1261 3074 Fragments arranged by size: 3074 2305 1261 552 111 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2330 3494 4465 5330 5347 5500 5685 Size: 1255 750 1164 971 865 17 153 185 Cuts at: 5685 6130 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1164 971 865 750 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2334 2775 5549 Size: 274 80 33 51 1443 357 441 2774 Cuts at: 5549 7261 96 Size: 1712 138 Fragments arranged by size: 2774 1712 1443 441 357 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2857 2889 7263 7295 2857 Size: 32 4374 32 2865 Fragments arranged by size: 4374 2865 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7303 XbaI T'CTAG_A Cuts at: 3449 4639 3449 Size: 1190 6113 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2029 3790 2029 Size: 1761 5542 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7303 XmaI C'CCGG_G Cuts at: 1870 2076 4628 1870 Size: 206 2552 4545 Fragments arranged by size: 4545 2552 206 XmnI GAAnn'nnTTC Cuts at: 3267 5432 3267 Size: 2165 5138 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5111 276 Size: 53 83 186 936 3577 2468 Fragments arranged by size: 3577 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SwaI