Restriction map for
pFN21ASDA0201
(Linear) (Six-base) MAPSORT of: KIAA0201.seq Check: 1499 from: 1 to: 2574
KIAA0201.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 1259 1550 2036 2147 2181 2574
Size: 394 865 291 486 111 34 393
Fragments arranged by size:
865 486 394 393 291 111 34
AflIII A'CryG_T
Cuts at: 0 1835 2383 2574
Size: 1835 548 191
Fragments arranged by size:
1835 548 191
AgeI A'CCGG_T
Cuts at: 0 95 2574
Size: 95 2479
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2192 2574
Size: 2192 382
AlwNI CAG_nnn'CTG
Cuts at: 0 451 1579 1708 2014 2166 2574
Size: 451 1128 129 306 152 408
Fragments arranged by size:
1128 451 408 306 152 129
ApaLI G'TGCA_C
Cuts at: 0 99 2574
Size: 99 2475
ApoI r'AATT_y
Cuts at: 0 545 739 1037 1066 1171 1963 1989 2550
Size: 545 194 298 29 105 792 26 561
Cuts at: 2550 2574
Size: 24
Fragments arranged by size:
792 561 545 298 194 105 29 26
24
AseI AT'TA_AT
Cuts at: 0 2083 2574
Size: 2083 491
AvaI C'yCGr_G
Cuts at: 0 53 2574
Size: 53 2521
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 781 814 2574
Size: 781 33 1760
Fragments arranged by size:
1760 781 33
BamHI G'GATC_C
Cuts at: 0 2328 2574
Size: 2328 246
BanII G_rGCy'C
Cuts at: 0 28 835 2574
Size: 28 807 1739
Fragments arranged by size:
1739 807 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 1517 2024 2492 2574
Size: 1517 507 468 82
Fragments arranged by size:
1517 507 468 82
BclI T'GATC_A
Cuts at: 0 2322 2574
Size: 2322 252
Bme1580I G_kGCm'C
Cuts at: 0 103 2574
Size: 103 2471
BmgBI CAC'GTC
Cuts at: 0 21 2574
Size: 21 2553
BmrI ACTGGGnnnn_n'
Cuts at: 0 676 2574
Size: 676 1898
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1727 2084 2574
Size: 1727 357 490
Fragments arranged by size:
1727 490 357
Bpu10I CC'TnA_GC
Cuts at: 0 2303 2345 2574
Size: 2303 42 229
Fragments arranged by size:
2303 229 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 382 2574
Size: 279 103 2192
Fragments arranged by size:
2192 279 103
BsaI GGTCTCn'nnnn_
Cuts at: 0 64 1551 2574
Size: 64 1487 1023
Fragments arranged by size:
1487 1023 64
BsaAI yAC'GTr
Cuts at: 0 2341 2574
Size: 2341 233
BsaBI GATnn'nnATC
Cuts at: 0 2327 2574
Size: 2327 247
BsaWI w'CCGG_w
Cuts at: 0 95 892 2574
Size: 95 797 1682
Fragments arranged by size:
1682 797 95
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 770 800 2574
Size: 770 30 1774
Fragments arranged by size:
1774 770 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1525 1993 2574
Size: 1525 468 581
Fragments arranged by size:
1525 581 468
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 461 755 1028 1388 2574
Size: 461 294 273 360 1186
Fragments arranged by size:
1186 461 360 294 273
BsiEI CG_ry'CG
Cuts at: 0 96 2574
Size: 96 2478
BsiHKAI G_wGCw'C
Cuts at: 0 103 786 835 2346 2574
Size: 103 683 49 1511 228
Fragments arranged by size:
1511 683 228 103 49
BsmI GAATG_Cn'
Cuts at: 0 220 871 2304 2574
Size: 220 651 1433 270
Fragments arranged by size:
1433 651 270 220
Bsp1286I G_dGCh'C
Cuts at: 0 28 103 786 835 2346 2574
Size: 28 75 683 49 1511 228
Fragments arranged by size:
1511 683 228 75 49 28
BspEI T'CCGG_A
Cuts at: 0 892 2574
Size: 892 1682
BspHI T'CATG_A
Cuts at: 0 1248 2294 2574
Size: 1248 1046 280
Fragments arranged by size:
1248 1046 280
BsrFI r'CCGG_y
Cuts at: 0 95 2574
Size: 95 2479
BsrGI T'GTAC_A
Cuts at: 0 311 2574
Size: 311 2263
BstYI r'GATC_y
Cuts at: 0 558 2328 2574
Size: 558 1770 246
Fragments arranged by size:
1770 558 246
BtgI C'CryG_G
Cuts at: 0 1458 2574
Size: 1458 1116
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 27 60 2574
Size: 27 33 2514
Fragments arranged by size:
2514 33 27
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1929 1964 2574
Size: 1929 35 610
Fragments arranged by size:
1929 610 35
DraI TTT'AAA
Cuts at: 0 744 1161 2574
Size: 744 417 1413
Fragments arranged by size:
1413 744 417
EaeI y'GGCC_r
Cuts at: 0 213 2134 2574
Size: 213 1921 440
Fragments arranged by size:
1921 440 213
EcoICRI GAG'CTC
Cuts at: 0 833 2574
Size: 833 1741
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 1259 1550 1727 2036 2084 2147 2181
Size: 394 865 291 177 309 48 63 34
Cuts at: 2181 2574
Size: 393
Fragments arranged by size:
865 394 393 309 291 177 63 48
34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 689 2574
Size: 689 1885
EcoO109I rG'GnC_Cy
Cuts at: 0 1312 2574
Size: 1312 1262
EcoRI G'AATT_C
Cuts at: 0 1037 2574
Size: 1037 1537
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 273 976 1331 1846 2574
Size: 273 703 355 515 728
Fragments arranged by size:
728 703 515 355 273
HaeII r_GCGC'y
Cuts at: 0 1031 2574
Size: 1031 1543
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 445 477 754 786 1421 1453
Size: 10 32 403 32 277 32 635 32
Cuts at: 1453 2307 2339 2574
Size: 854 32 235
Fragments arranged by size:
854 635 403 277 235 32 32 32
32 32 10
HincII GTy'rAC
Cuts at: 0 365 1452 1734 2067 2574
Size: 365 1087 282 333 507
Fragments arranged by size:
1087 507 365 333 282
HindIII A'AGCT_T
Cuts at: 0 1328 2574
Size: 1328 1246
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 276 740 953 1002 2054 2574
Size: 276 464 213 49 1052 520
Fragments arranged by size:
1052 520 464 276 213 49
MslI CAynn'nnrTG
Cuts at: 0 731 1134 2228 2522 2574
Size: 731 403 1094 294 52
Fragments arranged by size:
1094 731 403 294 52
MspA1I CmG'CkG
Cuts at: 0 526 1045 2191 2574
Size: 526 519 1146 383
Fragments arranged by size:
1146 526 519 383
NcoI C'CATG_G
Cuts at: 0 1458 2574
Size: 1458 1116
NdeI CA'TA_TG
Cuts at: 0 1956 2061 2574
Size: 1956 105 513
Fragments arranged by size:
1956 513 105
NspI r_CATG'y
Cuts at: 0 1839 2387 2574
Size: 1839 548 187
Fragments arranged by size:
1839 548 187
PciI A'CATG_T
Cuts at: 0 1835 2383 2574
Size: 1835 548 191
Fragments arranged by size:
1835 548 191
PsiI TTA'TAA
Cuts at: 0 551 2574
Size: 551 2023
PstI C_TGCA'G
Cuts at: 0 2171 2574
Size: 2171 403
PvuII CAG'CTG
Cuts at: 0 526 1045 2191 2574
Size: 526 519 1146 383
Fragments arranged by size:
1146 526 519 383
SacI G_AGCT'C
Cuts at: 0 835 2574
Size: 835 1739
SfcI C'TryA_G
Cuts at: 0 2167 2574
Size: 2167 407
SmaI CCC'GGG
Cuts at: 0 55 2574
Size: 55 2519
SmlI C'TyrA_G
Cuts at: 0 258 397 2574
Size: 258 139 2177
Fragments arranged by size:
2177 258 139
SrfI GCCC'GGGC
Cuts at: 0 55 2574
Size: 55 2519
SspI AAT'ATT
Cuts at: 0 2460 2574
Size: 2460 114
StyI C'CwwG_G
Cuts at: 0 1347 1458 2574
Size: 1347 111 1116
Fragments arranged by size:
1347 1116 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 307 1471 2442 2574
Size: 307 1164 971 132
Fragments arranged by size:
1164 971 307 132
TatI w'GTAC_w
Cuts at: 0 311 752 2574
Size: 311 441 1822
Fragments arranged by size:
1822 441 311
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 834 866 2574
Size: 834 32 1708
Fragments arranged by size:
1708 834 32
XbaI T'CTAG_A
Cuts at: 0 1426 2574
Size: 1426 1148
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1767 2574
Size: 1767 807
XmaI C'CCGG_G
Cuts at: 0 53 2574
Size: 53 2521
XmnI GAAnn'nnTTC
Cuts at: 0 1244 2574
Size: 1244 1330
Enzymes that do cut:
AcuI AflIII AgeI AlfI AlwNI ApaLI ApoI AseI
AvaI BaeI BamHI BanII BbsI BclI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI
BsaXI BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BsrFI BsrGI BstYI BtgI BtgZI CspCI DraI
EaeI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I HincII HindIII MmeI MslI MspA1I NcoI NdeI
NspI PciI PsiI PstI PvuII SacI SfcI SmaI
SmlI SrfI SspI StyI TaqII TatI TstI XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AhdI
AleI AloI ApaI AscI AsiSI AvrII BanI BbeI
BbvCI BcgI BciVI BfrBI BglI BglII BlpI BmtI
BplI BsaHI BseYI BsiWI BsmBI BspMI BsrBI BsrDI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtsI ClaI DraIII DrdI EagI EarI EciI EcoRV
FseI FspI FspAI HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI StuI SwaI Tth111I XhoI
ZraI