Restriction map for
pFN21ASDA0199
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# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AvrII BamHI
BbeI BciVI BclI BfrI BglII
BlnI BsiWI BspDI BspLU11I BsrDI
BssHII BstAPI BstBI ClaI CpoI
DraI Eam1105I Eco47III EcoNI EcoT22I
FbaI FseI HincII HindII HpaI
KasI KpnI KspI MfeI MluI
MunI NarI NdeI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
TfiI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AccIII 386 TCCGGA T^CCGGA
AcsI 166 GAATTC [GA]^AATT[CT]
ApoI 166 GAATTC [GA]^AATT[CT]
AspI 919 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1616 TGCGCA TGC^GCA
BsaBI 1581 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 386 TCCGGA [AT]^CCGG[AT]
BseAI 386 TCCGGA T^CCGGA
BspEI 386 TCCGGA T^CCGGA
BspHI 1175 TCATGA T^CATGA
Bst1107I 1594 GTATAC GTA^TAC
BstEII 1842 GGTAACC G^GT[ACGT]ACC
BstPI 1842 GGTAACC G^GT[ACGT]ACC
BstZ17I 1594 GTATAC GTA^TAC
DraIII 3214 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1825 GACAGCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1842 GGTAACC G^GT[ACGT]ACC
EcoRI 166 GAATTC G^AATTC
EcoRV 30 GATATC GAT^ATC
FspI 1616 TGCGCA TGC^GCA
HindIII 3612 AAGCTT A^AGCTT
MamI 1581 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 386 TCCGGA T^CCGGA
MseI 2626 TTAA T^TAA
NheI 1344 GCTAGC G^CTAGC
PflFI 919 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1175 TCATGA T^CATGA
SapI 1905 GCTCTTC GCTCTTC(1/4)
SmlI 2482 CTTGAG C^T[CT][GA]AG
Tru9I 2626 TTAA T^TAA
Tth111I 919 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AatII 1773 GACGTC GACGT^C
2113 GACGTC
Alw44I 820 GTGCAC G^TGCAC
3227 GTGCAC
ApaLI 820 GTGCAC G^TGCAC
3227 GTGCAC
BbrPI 394 CACGTG CAC^GTG
817 CACGTG
BbsI 715 GTCTTC GAAGAC(2/6)
1476 GTCTTC
BbvCI 1388 CCTCAGC CCTCAGC(-5/-2)
3117 CCTCAGC
BpuAI 715 GTCTTC GAAGAC(2/6)
1476 GTCTTC
BsaAI 394 CACGTG [CT]AC^GT[GA]
817 CACGTG
BsmBI 2111 GAGACG CGTCTC(1/5)
2290 GAGACG
BsmI 364 GCATTC GAATGC(1/-1)
2432 GCATTC
BssSI 592 CACGAG CACGAG(-5/-1)
3324 CTCGTG
BstYI 383 AGATCC [GA]^GATC[CT]
3746 AGATCC
MflI 383 AGATCC [GA]^GATC[CT]
3746 AGATCC
NaeI 2006 GCCGGC GCC^GGC
2960 GCCGGC
NgoMIV 2006 GCCGGC G^CCGGC
2960 GCCGGC
NspI 1697 GCATGC [GA]CATG^[CT]
2357 GCATGC
PmaCI 394 CACGTG CAC^GTG
817 CACGTG
PmlI 394 CACGTG CAC^GTG
817 CACGTG
SacI 850 GAGCTC GAGCT^C
3793 GAGCTC
SphI 1697 GCATGC GCATG^C
2357 GCATGC
TaqI 917 TCGA T^CGA
2336 TCGA
TthHB8I 917 TCGA T^CGA
2336 TCGA
XhoII 383 AGATCC [GA]^GATC[CT]
3746 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcgI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrBI BsrFI BsrGI BsrI BssKI
BstF5I BstNI BstUI BstXI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SspBI
StuI StyI TseI Tsp45I Tsp509I
TspRI Van91I XcmI XmaCI XmaI