(Circular) (Six-base) MAPSORT of: pFN21ASDA0199.seq Check: 6173 from: 1 to: 8566 pFN21ASDA0199 8566 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4652 4652 Size: 8566 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3800 4140 6376 278 Size: 53 83 186 936 2264 340 2236 2468 Fragments arranged by size: 2468 2264 2236 936 340 186 83 53 AccI GT'mk_AC Cuts at: 1937 3141 3543 3618 5730 5909 1937 Size: 1204 402 75 2112 179 4594 Fragments arranged by size: 4594 2112 1204 402 179 75 Acc65I G'GTAC_C Cuts at: 5887 5887 Size: 8566 AclI AA'CG_TT Cuts at: 1563 6693 7066 1563 Size: 5130 373 3063 Fragments arranged by size: 5130 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2622 3299 3916 4741 5351 6629 8013 8281 Size: 990 677 617 825 610 1278 1384 268 Cuts at: 8281 1632 Size: 1917 Fragments arranged by size: 1917 1384 1278 990 825 677 617 610 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8369 AflIII A'CryG_T Cuts at: 1203 7465 8442 1203 Size: 6262 977 1327 Fragments arranged by size: 6262 1327 977 AgeI A'CCGG_T Cuts at: 7397 7397 Size: 8566 AhdI GACnn_n'nnGTC Cuts at: 7295 7295 Size: 8566 AleI CACnn'nnGTG Cuts at: 1166 2201 3251 5393 1166 Size: 1035 1050 2142 4339 Fragments arranged by size: 4339 2142 1050 1035 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2769 1184 Size: 1585 6981 AlwNI CAG_nnn'CTG Cuts at: 2183 3322 4056 5574 5620 5773 6309 7881 2183 Size: 1139 734 1518 46 153 536 1572 2868 Fragments arranged by size: 2868 1572 1518 1139 734 536 153 46 ApaI G_GGCC'C Cuts at: 4074 4112 4257 4604 4912 4074 Size: 38 145 347 308 7728 Fragments arranged by size: 7728 347 308 145 38 ApaLI G'TGCA_C Cuts at: 2843 5250 6623 7779 2843 Size: 2407 1373 1156 3630 Fragments arranged by size: 3630 2407 1373 1156 ApoI r'AATT_y Cuts at: 1493 2189 5875 6101 6253 8362 1493 Size: 696 3686 226 152 2109 1697 Fragments arranged by size: 3686 2109 1697 696 226 152 AseI AT'TA_AT Cuts at: 160 1032 7120 160 Size: 872 6088 1606 Fragments arranged by size: 6088 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8566 AvaI C'yCGr_G Cuts at: 1870 1943 2058 2382 4009 4256 4310 4596 4700 Size: 73 115 324 1627 247 54 286 104 Cuts at: 4700 4734 4880 5891 1870 Size: 34 146 1011 4545 Fragments arranged by size: 4545 1627 1011 324 286 247 146 115 104 73 54 34 BamHI G'GATC_C Cuts at: 5896 6263 5896 Size: 367 8199 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2282 3590 4032 4710 5887 Size: 325 217 627 495 1308 442 678 1177 Cuts at: 5887 7342 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1308 1177 678 627 495 442 325 217 BanII G_rGCy'C Cuts at: 1391 2082 2877 3677 3694 4074 4112 4257 4604 Size: 691 795 800 17 380 38 145 347 Cuts at: 4604 4707 4912 5820 5885 1391 Size: 103 205 908 65 4072 Fragments arranged by size: 4072 908 800 795 691 380 347 205 145 103 65 38 17 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2731 3492 928 Size: 971 832 761 6002 Fragments arranged by size: 6002 971 832 761 BbvCI CC'TCA_GC Cuts at: 3412 5141 3412 Size: 1729 6837 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3013 3047 3631 3665 4942 4976 6757 Size: 34 1076 34 584 34 1277 34 1781 Cuts at: 6757 6791 1903 Size: 34 3678 Fragments arranged by size: 3678 1781 1277 1076 584 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6458 7668 6458 Size: 1210 7356 BclI T'GATC_A Cuts at: 1548 1548 Size: 8566 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2291 4261 4407 Size: 107 122 71 631 605 619 1970 146 Cuts at: 4407 4599 7177 136 Size: 192 2578 1525 Fragments arranged by size: 2578 1970 1525 631 619 605 192 146 122 107 71 BglII A'GATC_T Cuts at: 8561 8561 Size: 8566 BlpI GC'TnA_GC Cuts at: 2871 2993 5819 5983 2871 Size: 122 2826 164 5454 Fragments arranged by size: 5454 2826 164 122 Bme1580I G_kGCm'C Cuts at: 1790 2287 2782 2847 3258 4074 4089 4112 4257 Size: 497 495 65 411 816 15 23 145 Cuts at: 4257 4604 4912 5247 5254 5838 6627 7783 1790 Size: 347 308 335 7 584 789 1156 2573 Fragments arranged by size: 2573 1156 816 789 584 497 495 411 347 335 308 145 65 23 15 7 BmgBI CAC'GTC Cuts at: 1318 4414 1318 Size: 3096 5470 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2575 3848 3983 Size: 416 53 83 399 24 1158 1273 135 Cuts at: 3983 4099 4908 5313 6297 7255 442 Size: 116 809 405 984 958 1753 Fragments arranged by size: 1753 1273 1158 984 958 809 416 405 399 135 116 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3371 1056 Size: 2315 6251 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4035 4891 4906 5056 5059 4035 Size: 856 15 150 3 7542 Fragments arranged by size: 7542 856 150 15 3 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3167 4043 4954 5009 5178 5360 5867 7226 Size: 1928 876 911 55 169 182 507 1359 Cuts at: 7226 1239 Size: 2579 Fragments arranged by size: 2579 1928 1359 911 876 507 182 169 55 Bpu10I CC'TnA_GC Cuts at: 2501 2517 3176 3412 3777 3816 4512 5141 2501 Size: 16 659 236 365 39 696 629 5926 Fragments arranged by size: 5926 696 659 629 365 236 39 16 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4526 6691 7556 7854 4526 Size: 2165 865 298 5238 Fragments arranged by size: 5238 2165 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2722 3133 5044 5120 7229 882 Size: 617 1223 411 1911 76 2109 2219 Fragments arranged by size: 2219 2109 1911 1223 617 411 76 BsaAI yAC'GTr Cuts at: 493 1204 2419 2842 5863 6357 493 Size: 711 1215 423 3021 494 2702 Fragments arranged by size: 3021 2702 1215 711 494 423 BsaBI GATnn'nnATC Cuts at: 3608 8560 3608 Size: 4952 3614 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3041 3797 4137 5001 Size: 53 83 186 936 1508 756 340 864 Cuts at: 5001 6373 6755 7390 275 Size: 1372 382 635 1451 Fragments arranged by size: 1508 1451 1372 936 864 756 635 382 340 186 83 53 BsaWI w'CCGG_w Cuts at: 2409 6999 7397 7671 7818 8229 2409 Size: 4590 398 274 147 411 2746 Fragments arranged by size: 4590 2746 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2444 2474 5333 5363 1178 Size: 30 165 30 1041 30 2859 30 4381 Fragments arranged by size: 4381 2859 1041 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2072 2417 2468 2870 4574 4802 5032 5048 Size: 886 345 51 402 1704 228 230 16 Cuts at: 5048 1186 Size: 4704 Fragments arranged by size: 4704 1704 886 402 345 230 228 51 16 BseYI C'CCAG_C Cuts at: 2801 3220 3628 3731 4082 4162 4243 4521 5288 Size: 419 408 103 351 80 81 278 767 Cuts at: 5288 5754 7769 8265 2801 Size: 466 2015 496 3102 Fragments arranged by size: 3102 2015 767 496 466 419 408 351 278 103 81 80 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2780 3007 4142 4790 4792 5073 1163 Size: 573 1044 227 1135 648 2 281 4656 Fragments arranged by size: 4656 1135 1044 648 573 281 227 2 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4048 4803 4984 5394 6016 6777 Size: 275 186 2047 755 181 410 622 761 Cuts at: 6777 7805 1540 Size: 1028 2301 Fragments arranged by size: 2301 2047 1028 761 755 622 410 275 186 181 BsiHKAI G_wGCw'C Cuts at: 2847 2877 2994 3114 5254 5820 6627 6712 7783 Size: 30 117 120 2140 566 807 85 1071 Cuts at: 7783 2847 Size: 3630 Fragments arranged by size: 3630 2140 1071 807 566 120 117 85 30 BsmI GAATG_Cn' Cuts at: 2387 4455 6077 6170 2387 Size: 2068 1622 93 4783 Fragments arranged by size: 4783 2068 1622 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4128 4307 4128 Size: 179 8387 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 2287 2782 2847 2877 2994 3114 Size: 399 292 205 495 65 30 117 120 Cuts at: 3114 3258 3677 3694 4074 4089 4112 4257 4604 Size: 144 419 17 380 15 23 145 347 Cuts at: 4604 4707 4912 5247 5254 5820 5838 5885 6627 Size: 103 205 335 7 566 18 47 742 Cuts at: 6627 6712 7783 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 566 495 419 399 380 347 335 292 205 205 145 144 120 117 103 85 65 47 30 23 18 17 15 7 BspEI T'CCGG_A Cuts at: 2409 8229 2409 Size: 5820 2746 BspHI T'CATG_A Cuts at: 3198 6455 8185 3198 Size: 3257 1730 3579 Fragments arranged by size: 3579 3257 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2487 3522 4087 4652 5667 5921 844 Size: 1643 1035 565 565 1015 254 3489 Fragments arranged by size: 3489 1643 1035 1015 565 565 254 BsrBI CCG'CTC Cuts at: 1394 1823 3448 4731 5057 6453 1394 Size: 429 1625 1283 326 1396 3507 Fragments arranged by size: 3507 1625 1396 1283 429 326 BsrDI GCAATG_nn' Cuts at: 65 1248 7061 7235 65 Size: 1183 5813 174 1396 Fragments arranged by size: 5813 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4029 4175 4840 4983 7210 7397 1700 Size: 110 2219 146 665 143 2227 187 2869 Fragments arranged by size: 2869 2227 2219 665 187 146 143 110 BsrGI T'GTAC_A Cuts at: 96 4146 5199 5397 96 Size: 4050 1053 198 3265 Fragments arranged by size: 4050 3265 1053 198 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8566 BssSI C'ACGA_G Cuts at: 2615 5347 6626 7638 2615 Size: 2732 1279 1012 3543 Fragments arranged by size: 3543 2732 1279 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8566 BstBI TT'CG_AA Cuts at: 2007 7370 2007 Size: 5363 3203 BstEII G'GTnAC_C Cuts at: 1184 3865 1184 Size: 2681 5885 BstXI CCAn_nnnn'nTGG Cuts at: 2734 2764 3124 3220 3424 5419 2734 Size: 30 360 96 204 1995 5881 Fragments arranged by size: 5881 1995 360 204 96 30 BstYI r'GATC_y Cuts at: 1972 2406 5769 5896 6263 6648 6665 8117 8203 Size: 434 3363 127 367 385 17 1452 86 Cuts at: 8203 8215 8561 1972 Size: 12 346 1977 Fragments arranged by size: 3363 1977 1452 434 385 367 346 127 86 17 12 BstZ17I GTA'TAC Cuts at: 3619 3619 Size: 8566 Bsu36I CC'TnA_GG Cuts at: 2492 3396 3879 4038 4860 5044 2492 Size: 904 483 159 822 184 6014 Fragments arranged by size: 6014 904 822 483 184 159 BtgI C'CryG_G Cuts at: 513 1065 2074 2979 3213 4129 5412 5500 513 Size: 552 1009 905 234 916 1283 88 3579 Fragments arranged by size: 3579 1283 1009 916 905 552 234 88 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7827 Fragments arranged by size: 7827 683 56 BtsI GCAGTG_nn' Cuts at: 2150 4057 5133 5554 5818 6082 6876 6896 2150 Size: 1907 1076 421 264 264 794 20 3820 Fragments arranged by size: 3820 1907 1076 794 421 264 264 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8566 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8531 DraI TTT'AAA Cuts at: 5870 5870 Size: 8566 DraIII CAC_nnn'GTG Cuts at: 5242 5242 Size: 8566 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3854 7573 809 Size: 507 2538 3719 1802 Fragments arranged by size: 3719 2538 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3210 3920 3954 Size: 54 1425 50 275 18 1380 710 34 Cuts at: 3954 4045 4170 4342 4368 4726 4800 4981 5421 Size: 91 125 172 26 358 74 181 440 Cuts at: 5421 5674 6013 6902 8531 8 Size: 253 339 889 1629 43 Fragments arranged by size: 1629 1425 1380 889 710 440 358 339 275 253 181 172 125 91 74 54 50 43 34 26 18 EagI C'GGCC_G Cuts at: 1537 1812 4045 4800 4981 6013 1537 Size: 275 2233 755 181 1032 4090 Fragments arranged by size: 4090 2233 1032 755 275 181 EarI CTCTTCn'nnn_ Cuts at: 1353 2120 2702 3035 3935 4625 5363 6496 1353 Size: 767 582 333 900 690 738 1133 3423 Fragments arranged by size: 3423 1133 900 767 738 690 582 333 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2840 3902 3989 4279 4596 4987 Size: 1064 138 1251 1062 87 290 317 391 Cuts at: 4987 7153 7527 7673 387 Size: 2166 374 146 1280 Fragments arranged by size: 2166 1280 1251 1064 1062 391 374 317 290 146 138 87 EcoICRI GAG'CTC Cuts at: 2875 5818 2875 Size: 2943 5623 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2622 3167 3299 3916 4043 4741 4954 Size: 393 990 545 132 617 127 698 213 Cuts at: 4954 5009 5178 5351 5360 5867 6629 7226 8013 Size: 55 169 173 9 507 762 597 787 Cuts at: 8013 8281 1239 Size: 268 1524 Fragments arranged by size: 1524 990 787 762 698 617 597 545 507 393 268 213 173 169 132 127 55 9 EcoO109I rG'GnC_Cy Cuts at: 2180 3875 4070 4100 4108 4600 4601 4855 4909 Size: 1695 195 30 8 492 1 254 54 Cuts at: 4909 5163 5617 5698 5713 2180 Size: 254 454 81 15 5033 Fragments arranged by size: 5033 1695 492 454 254 254 195 81 54 30 15 8 1 EcoRI G'AATT_C Cuts at: 2189 5875 6253 2189 Size: 3686 378 4502 Fragments arranged by size: 4502 3686 378 EcoRV GAT'ATC Cuts at: 2055 2055 Size: 8566 FspI TGC'GCA Cuts at: 3641 7072 3641 Size: 3431 5135 HaeII r_GCGC'y Cuts at: 1678 3577 4163 4480 5296 7713 1678 Size: 1899 586 317 816 2417 2531 Fragments arranged by size: 2531 2417 1899 816 586 317 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2039 2071 3176 3208 5102 Size: 32 135 32 467 32 1105 32 1894 Cuts at: 5102 5134 5498 5530 7207 7239 7281 7313 1373 Size: 32 364 32 1677 32 42 32 2626 Fragments arranged by size: 2626 1894 1677 1105 467 364 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5910 6156 669 Size: 988 281 3972 246 3079 Fragments arranged by size: 3972 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 5635 748 Size: 4887 3679 HpaI GTT'AAC Cuts at: 6156 6156 Size: 8566 KpnI G_GTAC'C Cuts at: 5891 5891 Size: 8566 MfeI C'AATT_G Cuts at: 6165 6165 Size: 8566 MluI A'CGCG_T Cuts at: 8442 8442 Size: 8566 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2416 7680 7864 1305 Size: 1111 5264 184 2007 Fragments arranged by size: 5264 2007 1111 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3212 3956 4172 4370 5423 Size: 54 1425 343 1380 744 216 198 1053 Cuts at: 5423 10 Size: 3153 Fragments arranged by size: 3153 1425 1380 1053 744 343 216 198 54 MslI CAynn'nnrTG Cuts at: 518 1166 2201 2751 3011 3251 3707 4964 5393 Size: 648 1035 550 260 240 456 1257 429 Cuts at: 5393 6524 6883 7042 518 Size: 1131 359 159 2042 Fragments arranged by size: 2042 1257 1131 1035 648 550 456 429 359 260 240 159 MspA1I CmG'CkG Cuts at: 1606 2685 3454 4243 4500 4761 4774 4980 5285 Size: 1079 769 789 257 261 13 206 305 Cuts at: 5285 5982 6659 7807 8052 1606 Size: 697 677 1148 245 2120 Fragments arranged by size: 2120 1148 1079 789 769 697 677 305 261 257 245 206 13 NaeI GCC'GGC Cuts at: 4031 4985 4031 Size: 954 7612 NcoI C'CATG_G Cuts at: 513 1065 2074 4129 5412 513 Size: 552 1009 2055 1283 3667 Fragments arranged by size: 3667 2055 1283 1009 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8566 NgoMIV G'CCGG_C Cuts at: 4029 4983 4029 Size: 954 7612 NheI G'CTAG_C Cuts at: 1052 3367 1052 Size: 2315 6251 NotI GC'GGCC_GC Cuts at: 6013 6013 Size: 8566 NspI r_CATG'y Cuts at: 1132 1229 3724 4384 5924 7469 1132 Size: 97 2495 660 1540 1545 2229 Fragments arranged by size: 2495 2229 1545 1540 660 97 PasI CC'CwG_GG Cuts at: 1348 2543 5168 1348 Size: 1195 2625 4746 Fragments arranged by size: 4746 2625 1195 PciI A'CATG_T Cuts at: 7465 7465 Size: 8566 PflMI CCAn_nnn'nTGG Cuts at: 1108 2398 4351 5413 5773 1108 Size: 1290 1953 1062 360 3901 Fragments arranged by size: 3901 1953 1290 1062 360 PfoI T'CCnGG_A Cuts at: 3264 3264 Size: 8566 PmeI GTTT'AAAC Cuts at: 5870 5870 Size: 8566 PmlI CAC'GTG Cuts at: 2419 2842 2419 Size: 423 8143 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6635 6667 8148 8180 6635 Size: 32 1481 32 7021 Fragments arranged by size: 7021 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2180 4100 5163 5617 5698 5713 2180 Size: 1920 1063 454 81 15 5033 Fragments arranged by size: 5033 1920 1063 454 81 15 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8566 PsiI TTA'TAA Cuts at: 6136 6136 Size: 8566 PspOMI G'GGCC_C Cuts at: 4070 4108 4253 4600 4908 4070 Size: 38 145 347 308 7728 Fragments arranged by size: 7728 347 308 145 38 PstI C_TGCA'G Cuts at: 830 2634 2816 4950 5027 5154 5918 830 Size: 1804 182 2134 77 127 764 3478 Fragments arranged by size: 3478 2134 1804 764 182 127 77 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8566 PvuII CAG'CTG Cuts at: 3454 4243 4761 3454 Size: 789 518 7259 Fragments arranged by size: 7259 789 518 SacI G_AGCT'C Cuts at: 2877 5820 2877 Size: 2943 5623 SalI G'TCGA_C Cuts at: 1936 5908 1936 Size: 3972 4594 SanDI GG'GwC_CC Cuts at: 5163 5163 Size: 8566 SapI GCTCTTCn'nnn_ Cuts at: 3935 3935 Size: 8566 SbfI CC_TGCA'GG Cuts at: 5918 5918 Size: 8566 ScaI AGT'ACT Cuts at: 6814 8526 6814 Size: 1712 6854 SfcI C'TryA_G Cuts at: 826 1045 2630 2812 4946 5023 5150 5914 7049 Size: 219 1585 182 2134 77 127 764 1135 Cuts at: 7049 7730 7921 826 Size: 681 191 1471 Fragments arranged by size: 2134 1585 1471 1135 764 681 219 191 182 127 77 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8566 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8566 SmaI CCC'GGG Cuts at: 1872 2384 4258 4598 5893 1872 Size: 512 1874 340 1295 4545 Fragments arranged by size: 4545 1874 1295 512 340 SmlI C'TyrA_G Cuts at: 820 1017 1943 4505 6670 7571 7833 820 Size: 197 926 2562 2165 901 262 1553 Fragments arranged by size: 2562 2165 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5863 6357 493 Size: 5370 494 2702 Fragments arranged by size: 5370 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8566 SphI G_CATG'C Cuts at: 1132 3724 4384 5924 1132 Size: 2592 660 1540 3774 Fragments arranged by size: 3774 2592 1540 660 SrfI GCCC'GGGC Cuts at: 4258 4258 Size: 8566 SspI AAT'ATT Cuts at: 5 52 6490 5 Size: 47 6438 2081 Fragments arranged by size: 6438 2081 47 StuI AGG'CCT Cuts at: 1517 2490 3174 3401 1517 Size: 973 684 227 6682 Fragments arranged by size: 6682 973 684 227 StyI C'CwwG_G Cuts at: 513 1065 2074 3169 4104 4129 5121 5412 6005 Size: 552 1009 1095 935 25 992 291 593 Cuts at: 6005 513 Size: 3074 Fragments arranged by size: 3074 1095 1009 992 935 593 552 291 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2270 2489 3671 4235 4668 5630 6593 Size: 1255 690 219 1182 564 433 962 963 Cuts at: 6593 6610 6763 6948 7393 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1182 963 962 690 564 445 433 219 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2679 4146 5199 Size: 274 80 33 51 1443 702 1467 1053 Cuts at: 5199 5397 5461 6812 8524 96 Size: 198 64 1351 1712 138 Fragments arranged by size: 1712 1467 1443 1351 1053 702 274 198 138 80 64 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2444 2476 4346 4378 5333 5365 5731 5763 8526 Size: 32 1870 32 955 32 366 32 2763 Cuts at: 8526 8558 2444 Size: 32 2452 Fragments arranged by size: 2763 2452 1870 955 366 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2945 1316 Size: 1629 6937 XbaI T'CTAG_A Cuts at: 5902 5902 Size: 8566 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2710 2962 3867 4936 2710 Size: 252 905 1069 6340 Fragments arranged by size: 6340 1069 905 252 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8566 XmaI C'CCGG_G Cuts at: 1870 2382 4256 4596 5891 1870 Size: 512 1874 340 1295 4545 Fragments arranged by size: 4545 1874 1295 512 340 XmnI GAAnn'nnTTC Cuts at: 6695 6695 Size: 8566 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3798 4138 6374 276 Size: 53 83 186 936 2264 340 2236 2468 Fragments arranged by size: 2468 2264 2236 936 340 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI EcoNI FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacII SexAI SfiI SfoI SwaI