Restriction map for
pFN21ASDA0199
(Linear) (Six-base) MAPSORT of: KIAA0199.seq Check: 1718 from: 1 to: 3837
KIAA0199.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2629 3837
Size: 2629 1208
AatII G_ACGT'C
Cuts at: 0 1777 2117 3837
Size: 1777 340 1720
Fragments arranged by size:
1777 1720 340
AccI GT'mk_AC
Cuts at: 0 1118 1520 1595 3707 3837
Size: 1118 402 75 2112 130
Fragments arranged by size:
2112 1118 402 130 75
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 599 1276 1893 2718 3328 3837
Size: 599 677 617 825 610 509
Fragments arranged by size:
825 677 617 610 599 509
AleI CACnn'nnGTG
Cuts at: 0 178 1228 3370 3837
Size: 178 1050 2142 467
Fragments arranged by size:
2142 1050 467 178
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 746 3837
Size: 746 3091
AlwNI CAG_nnn'CTG
Cuts at: 0 160 1299 2033 3551 3597 3750 3837
Size: 160 1139 734 1518 46 153 87
Fragments arranged by size:
1518 1139 734 160 153 87 46
ApaI G_GGCC'C
Cuts at: 0 2051 2089 2234 2581 2889 3837
Size: 2051 38 145 347 308 948
Fragments arranged by size:
2051 948 347 308 145 38
ApaLI G'TGCA_C
Cuts at: 0 820 3227 3837
Size: 820 2407 610
Fragments arranged by size:
2407 820 610
ApoI r'AATT_y
Cuts at: 0 166 3837
Size: 166 3671
AvaI C'yCGr_G
Cuts at: 0 35 359 1986 2233 2287 2573 2677 2711
Size: 35 324 1627 247 54 286 104 34
Cuts at: 2711 2857 3837
Size: 146 980
Fragments arranged by size:
1627 980 324 286 247 146 104 54
35 34
BanI G'GyrC_C
Cuts at: 0 259 1567 2009 2687 3837
Size: 259 1308 442 678 1150
Fragments arranged by size:
1308 1150 678 442 259
BanII G_rGCy'C
Cuts at: 0 59 854 1654 1671 2051 2089 2234 2581
Size: 59 795 800 17 380 38 145 347
Cuts at: 2581 2684 2889 3797 3837
Size: 103 205 908 40
Fragments arranged by size:
908 800 795 380 347 205 145 103
59 40 38 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 708 1469 3837
Size: 708 761 2368
Fragments arranged by size:
2368 761 708
BbvCI CC'TCA_GC
Cuts at: 0 1389 3118 3837
Size: 1389 1729 719
Fragments arranged by size:
1729 1389 719
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 990 1024 1608 1642 2919 2953 3837
Size: 990 34 584 34 1277 34 884
Fragments arranged by size:
1277 990 884 584 34 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 268 2238 2384 2576 3837
Size: 268 1970 146 192 1261
Fragments arranged by size:
1970 1261 268 192 146
BlpI GC'TnA_GC
Cuts at: 0 848 970 3796 3837
Size: 848 122 2826 41
Fragments arranged by size:
2826 848 122 41
Bme1580I G_kGCm'C
Cuts at: 0 264 759 824 1235 2051 2066 2089 2234
Size: 264 495 65 411 816 15 23 145
Cuts at: 2234 2581 2889 3224 3231 3815 3837
Size: 347 308 335 7 584 22
Fragments arranged by size:
816 584 495 411 347 335 308 264
145 65 23 22 15 7
BmgBI CAC'GTC
Cuts at: 0 2391 3837
Size: 2391 1446
BmrI ACTGGGnnnn_n'
Cuts at: 0 552 1825 1960 2076 2885 3290 3837
Size: 552 1273 135 116 809 405 547
Fragments arranged by size:
1273 809 552 547 405 135 116
BmtI G_CTAG'C
Cuts at: 0 1348 3837
Size: 1348 2489
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2012 2868 2883 3033 3036 3837
Size: 2012 856 15 150 3 801
Fragments arranged by size:
2012 856 801 150 15 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1144 2020 2931 2986 3155 3337 3837
Size: 1144 876 911 55 169 182 500
Fragments arranged by size:
1144 911 876 500 182 169 55
Bpu10I CC'TnA_GC
Cuts at: 0 478 494 1153 1389 1754 1793 2489 3118
Size: 478 16 659 236 365 39 696 629
Cuts at: 3118 3837
Size: 719
Fragments arranged by size:
719 696 659 629 478 365 236 39
16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2503 3837
Size: 2503 1334
BsaI GGTCTCn'nnnn_
Cuts at: 0 699 1110 3021 3097 3837
Size: 699 411 1911 76 740
Fragments arranged by size:
1911 740 699 411 76
BsaAI yAC'GTr
Cuts at: 0 396 819 3837
Size: 396 423 3018
Fragments arranged by size:
3018 423 396
BsaBI GATnn'nnATC
Cuts at: 0 1585 3837
Size: 1585 2252
BsaHI Gr'CG_yC
Cuts at: 0 1018 1774 2114 2978 3837
Size: 1018 756 340 864 859
Fragments arranged by size:
1018 864 859 756 340
BsaWI w'CCGG_w
Cuts at: 0 386 3837
Size: 386 3451
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 421 451 3310 3340 3837
Size: 421 30 2859 30 497
Fragments arranged by size:
2859 497 421 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 394 445 847 2551 2779 3009 3025
Size: 49 345 51 402 1704 228 230 16
Cuts at: 3025 3837
Size: 812
Fragments arranged by size:
1704 812 402 345 230 228 51 49
16
BseYI C'CCAG_C
Cuts at: 0 778 1197 1605 1708 2059 2139 2220 2498
Size: 778 419 408 103 351 80 81 278
Cuts at: 2498 3265 3731 3837
Size: 767 466 106
Fragments arranged by size:
778 767 466 419 408 351 278 106
103 81 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 984 2119 2767 2769 3050 3837
Size: 757 227 1135 648 2 281 787
Fragments arranged by size:
1135 787 757 648 281 227 2
BsiEI CG_ry'CG
Cuts at: 0 2025 2780 2961 3371 3837
Size: 2025 755 181 410 466
Fragments arranged by size:
2025 755 466 410 181
BsiHKAI G_wGCw'C
Cuts at: 0 824 854 971 1091 3231 3797 3837
Size: 824 30 117 120 2140 566 40
Fragments arranged by size:
2140 824 566 120 117 40 30
BsmI GAATG_Cn'
Cuts at: 0 364 2432 3837
Size: 364 2068 1405
Fragments arranged by size:
2068 1405 364
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2105 2284 3837
Size: 2105 179 1553
Fragments arranged by size:
2105 1553 179
Bsp1286I G_dGCh'C
Cuts at: 0 59 264 759 824 854 971 1091 1235
Size: 59 205 495 65 30 117 120 144
Cuts at: 1235 1654 1671 2051 2066 2089 2234 2581 2684
Size: 419 17 380 15 23 145 347 103
Cuts at: 2684 2889 3224 3231 3797 3815 3837
Size: 205 335 7 566 18 22
Fragments arranged by size:
566 495 419 380 347 335 205 205
145 144 120 117 103 65 59 30
23 22 18 17 15 7
BspEI T'CCGG_A
Cuts at: 0 386 3837
Size: 386 3451
BspHI T'CATG_A
Cuts at: 0 1175 3837
Size: 1175 2662
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 464 1499 2064 2629 3644 3837
Size: 464 1035 565 565 1015 193
Fragments arranged by size:
1035 1015 565 565 464 193
BsrBI CCG'CTC
Cuts at: 0 1425 2708 3034 3837
Size: 1425 1283 326 803
Fragments arranged by size:
1425 1283 803 326
BsrFI r'CCGG_y
Cuts at: 0 2006 2152 2817 2960 3837
Size: 2006 146 665 143 877
Fragments arranged by size:
2006 877 665 146 143
BsrGI T'GTAC_A
Cuts at: 0 2123 3176 3374 3837
Size: 2123 1053 198 463
Fragments arranged by size:
2123 1053 463 198
BssSI C'ACGA_G
Cuts at: 0 592 3324 3837
Size: 592 2732 513
Fragments arranged by size:
2732 592 513
BstEII G'GTnAC_C
Cuts at: 0 1842 3837
Size: 1842 1995
BstXI CCAn_nnnn'nTGG
Cuts at: 0 711 741 1101 1197 1401 3396 3837
Size: 711 30 360 96 204 1995 441
Fragments arranged by size:
1995 711 441 360 204 96 30
BstYI r'GATC_y
Cuts at: 0 383 3746 3837
Size: 383 3363 91
Fragments arranged by size:
3363 383 91
BstZ17I GTA'TAC
Cuts at: 0 1596 3837
Size: 1596 2241
Bsu36I CC'TnA_GG
Cuts at: 0 469 1373 1856 2015 2837 3021 3837
Size: 469 904 483 159 822 184 816
Fragments arranged by size:
904 822 816 483 469 184 159
BtgI C'CryG_G
Cuts at: 0 51 956 1190 2106 3389 3477 3837
Size: 51 905 234 916 1283 88 360
Fragments arranged by size:
1283 916 905 360 234 88 51
BtsI GCAGTG_nn'
Cuts at: 0 127 2034 3110 3531 3795 3837
Size: 127 1907 1076 421 264 42
Fragments arranged by size:
1907 1076 421 264 127 42
DraIII CAC_nnn'GTG
Cuts at: 0 3219 3837
Size: 3219 618
DrdI GACnn_nn'nnGTC
Cuts at: 0 1831 3837
Size: 1831 2006
EaeI y'GGCC_r
Cuts at: 0 1187 1897 1931 2022 2147 2319 2345 2703
Size: 1187 710 34 91 125 172 26 358
Cuts at: 2703 2777 2958 3398 3651 3837
Size: 74 181 440 253 186
Fragments arranged by size:
1187 710 440 358 253 186 181 172
125 91 74 34 26
EagI C'GGCC_G
Cuts at: 0 2022 2777 2958 3837
Size: 2022 755 181 879
Fragments arranged by size:
2022 879 755 181
EarI CTCTTCn'nnn_
Cuts at: 0 97 679 1012 1912 2602 3340 3837
Size: 97 582 333 900 690 738 497
Fragments arranged by size:
900 738 690 582 497 333 97
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 817 1879 1966 2256 2573 2964 3837
Size: 817 1062 87 290 317 391 873
Fragments arranged by size:
1062 873 817 391 317 290 87
EcoICRI GAG'CTC
Cuts at: 0 852 3795 3837
Size: 852 2943 42
Fragments arranged by size:
2943 852 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 599 1144 1276 1893 2020 2718 2931 2986
Size: 599 545 132 617 127 698 213 55
Cuts at: 2986 3155 3328 3337 3837
Size: 169 173 9 500
Fragments arranged by size:
698 617 599 545 500 213 173 169
132 127 55 9
EcoO109I rG'GnC_Cy
Cuts at: 0 157 1852 2047 2077 2085 2577 2578 2832
Size: 157 1695 195 30 8 492 1 254
Cuts at: 2832 2886 3140 3594 3675 3690 3837
Size: 54 254 454 81 15 147
Fragments arranged by size:
1695 492 454 254 254 195 157 147
81 54 30 15 8 1
EcoRI G'AATT_C
Cuts at: 0 166 3837
Size: 166 3671
EcoRV GAT'ATC
Cuts at: 0 32 3837
Size: 32 3805
FspI TGC'GCA
Cuts at: 0 1618 3837
Size: 1618 2219
HaeII r_GCGC'y
Cuts at: 0 1554 2140 2457 3273 3837
Size: 1554 586 317 816 564
Fragments arranged by size:
1554 816 586 564 317
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 16 48 1153 1185 3079 3111 3475 3507
Size: 16 32 1105 32 1894 32 364 32
Cuts at: 3507 3837
Size: 330
Fragments arranged by size:
1894 1105 364 330 32 32 32 32
16
HindIII A'AGCT_T
Cuts at: 0 3612 3837
Size: 3612 225
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 393 3837
Size: 393 3444
MscI TGG'CCA
Cuts at: 0 1189 1933 2149 2347 3400 3837
Size: 1189 744 216 198 1053 437
Fragments arranged by size:
1189 1053 744 437 216 198
MslI CAynn'nnrTG
Cuts at: 0 178 728 988 1228 1684 2941 3370 3837
Size: 178 550 260 240 456 1257 429 467
Fragments arranged by size:
1257 550 467 456 429 260 240 178
MspA1I CmG'CkG
Cuts at: 0 662 1431 2220 2477 2738 2751 2957 3262
Size: 662 769 789 257 261 13 206 305
Cuts at: 3262 3837
Size: 575
Fragments arranged by size:
789 769 662 575 305 261 257 206
13
NaeI GCC'GGC
Cuts at: 0 2008 2962 3837
Size: 2008 954 875
Fragments arranged by size:
2008 954 875
NcoI C'CATG_G
Cuts at: 0 51 2106 3389 3837
Size: 51 2055 1283 448
Fragments arranged by size:
2055 1283 448 51
NgoMIV G'CCGG_C
Cuts at: 0 2006 2960 3837
Size: 2006 954 877
Fragments arranged by size:
2006 954 877
NheI G'CTAG_C
Cuts at: 0 1344 3837
Size: 1344 2493
NspI r_CATG'y
Cuts at: 0 1701 2361 3837
Size: 1701 660 1476
Fragments arranged by size:
1701 1476 660
PasI CC'CwG_GG
Cuts at: 0 520 3145 3837
Size: 520 2625 692
Fragments arranged by size:
2625 692 520
PflMI CCAn_nnn'nTGG
Cuts at: 0 375 2328 3390 3750 3837
Size: 375 1953 1062 360 87
Fragments arranged by size:
1953 1062 375 360 87
PfoI T'CCnGG_A
Cuts at: 0 1241 3837
Size: 1241 2596
PmlI CAC'GTG
Cuts at: 0 396 819 3837
Size: 396 423 3018
Fragments arranged by size:
3018 423 396
PpuMI rG'GwC_Cy
Cuts at: 0 157 2077 3140 3594 3675 3690 3837
Size: 157 1920 1063 454 81 15 147
Fragments arranged by size:
1920 1063 454 157 147 81 15
PspOMI G'GGCC_C
Cuts at: 0 2047 2085 2230 2577 2885 3837
Size: 2047 38 145 347 308 952
Fragments arranged by size:
2047 952 347 308 145 38
PstI C_TGCA'G
Cuts at: 0 611 793 2927 3004 3131 3837
Size: 611 182 2134 77 127 706
Fragments arranged by size:
2134 706 611 182 127 77
PvuII CAG'CTG
Cuts at: 0 1431 2220 2738 3837
Size: 1431 789 518 1099
Fragments arranged by size:
1431 1099 789 518
SacI G_AGCT'C
Cuts at: 0 854 3797 3837
Size: 854 2943 40
Fragments arranged by size:
2943 854 40
SanDI GG'GwC_CC
Cuts at: 0 3140 3837
Size: 3140 697
SapI GCTCTTCn'nnn_
Cuts at: 0 1912 3837
Size: 1912 1925
SfcI C'TryA_G
Cuts at: 0 607 789 2923 3000 3127 3837
Size: 607 182 2134 77 127 710
Fragments arranged by size:
2134 710 607 182 127 77
SmaI CCC'GGG
Cuts at: 0 361 2235 2575 3837
Size: 361 1874 340 1262
Fragments arranged by size:
1874 1262 361 340
SmlI C'TyrA_G
Cuts at: 0 2482 3837
Size: 2482 1355
SphI G_CATG'C
Cuts at: 0 1701 2361 3837
Size: 1701 660 1476
Fragments arranged by size:
1701 1476 660
SrfI GCCC'GGGC
Cuts at: 0 2235 3837
Size: 2235 1602
StuI AGG'CCT
Cuts at: 0 467 1151 1378 3837
Size: 467 684 227 2459
Fragments arranged by size:
2459 684 467 227
StyI C'CwwG_G
Cuts at: 0 51 1146 2081 2106 3098 3389 3837
Size: 51 1095 935 25 992 291 448
Fragments arranged by size:
1095 992 935 448 291 51 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 247 466 1648 2212 2645 3607 3837
Size: 247 219 1182 564 433 962 230
Fragments arranged by size:
1182 962 564 433 247 230 219
TatI w'GTAC_w
Cuts at: 0 656 2123 3176 3374 3438 3837
Size: 656 1467 1053 198 64 399
Fragments arranged by size:
1467 1053 656 399 198 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 421 453 2323 2355 3310 3342 3708 3740
Size: 421 32 1870 32 955 32 366 32
Cuts at: 3740 3837
Size: 97
Fragments arranged by size:
1870 955 421 366 97 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 922 3837
Size: 922 2915
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 687 939 1844 2913 3837
Size: 687 252 905 1069 924
Fragments arranged by size:
1069 924 905 687 252
XmaI C'CCGG_G
Cuts at: 0 359 2233 2573 3837
Size: 359 1874 340 1264
Fragments arranged by size:
1874 1264 359 340
ZraI GAC'GTC
Cuts at: 0 1775 2115 3837
Size: 1775 340 1722
Fragments arranged by size:
1775 1722 340
Enzymes that do cut:
AarI AatII AccI AcuI AleI AlfI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbsI BbvCI BcgI
BglI BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssSI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtsI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HindIII MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NheI NspI PasI PflMI
PfoI PmlI PpuMI PspOMI PstI PvuII SacI SanDI
SapI SfcI SmaI SmlI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI
BclI BfrBI BglII BsiWI BsrDI BssHII BstAPI BstBI
BtgZI ClaI CspCI DraI EcoNI FalI FseI FspAI
HincII HpaI KasI KpnI MfeI MluI NarI NdeI
NotI NruI NsiI PacI PciI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SspI SwaI XbaI XhoI XmnI